Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,200,918 T→A 5.7% M39L (ATG→TTG)  xisE ← e14 prophage; putative excisionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,200,9180TA 5.7% 432.8 / 10.7 150M39L (ATG→TTG) xisEe14 prophage; putative excisionase
Reads supporting (aligned to +/- strand):  ref base T (70/71);  new base A (5/4);  total (75/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 152 total aligned reads displayed.

GGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAG  >  NC_000913/1200769‑1201066
                                                                                                                                                     |                                                                                                                                                     
gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                                                                       <  1:1673004/150‑1 (MQ=255)
 ggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATg                                                                                                                                                      <  1:1044597/150‑1 (MQ=255)
    cTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCCCGCACTCGCCATAACCGGCCTTGTTTCATGGcc                                                                                                                                                   >  2:460393/1‑150 (MQ=255)
       aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGt                                                                                                                                                <  2:323632/150‑1 (MQ=255)
       aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGAAACAAGGCCGGt                                                                                                                                                <  2:1248138/150‑1 (MQ=255)
         aCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                                                                              <  2:97958/150‑1 (MQ=255)
         aCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                                                                              >  1:570234/1‑150 (MQ=255)
          cATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATCGCCGGTggc                                                                                                                                             >  1:296975/1‑149 (MQ=255)
          cATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTAGCAAAAGAAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGa                                                                                                                                             <  1:1610162/150‑1 (MQ=255)
           attattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                                                                            <  1:1685413/150‑1 (MQ=255)
            ttattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACa                                                                                                                                           >  2:230507/1‑150 (MQ=255)
              attCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                                                                         >  1:57567/1‑150 (MQ=255)
                tCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                       >  1:1707058/1‑150 (MQ=255)
                tCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTATCTTGATTACAGGTGTGACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                       <  1:1341723/150‑1 (MQ=255)
                tCTTACGCGGTCTGGCGGCCATTACTCAACATCCGGTGCAAAAGTAATGAGTCAGTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCTCGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                       <  1:1349230/150‑1 (MQ=255)
                 cTTACTCGTTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaata                                                                                                                                      <  2:238718/150‑1 (MQ=255)
                 cTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACACCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaata                                                                                                                                      >  2:68662/1‑150 (MQ=255)
                  ttACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataa                                                                                                                                     >  2:1022022/1‑150 (MQ=255)
                     cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                                                                  <  1:141546/150‑1 (MQ=255)
                      gcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTc                                                                                                                                 >  2:203244/1‑150 (MQ=255)
                          tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCCCGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                                                                             >  2:945801/1‑150 (MQ=255)
                           cTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCtt                                                                                                                            >  1:751946/1‑150 (MQ=255)
                             ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                          <  2:1369811/150‑1 (MQ=255)
                             ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                          >  1:1514196/1‑150 (MQ=255)
                              gCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGc                                                                                                                         <  2:608542/150‑1 (MQ=255)
                               cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCa                                                                                                                        <  1:1040431/150‑1 (MQ=255)
                                  ccATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATaa                                                                                                                     >  2:249019/1‑150 (MQ=255)
                                   cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                                    >  2:1078645/1‑150 (MQ=255)
                                      tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                                                                                 <  1:1177660/150‑1 (MQ=255)
                                      tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                                                                                 >  1:519567/1‑150 (MQ=255)
                                       aCTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGCACAAAATAAATTCTGCTTATCATAacgacg                                                                                                                >  2:311952/1‑150 (MQ=255)
                                        cTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCGCAACTACCTCGGCGTCCTCACGCACTCGCCATAACCTGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                                                                               <  2:732477/150‑1 (MQ=255)
                                        cTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                                                                               >  1:856637/1‑150 (MQ=255)
                                        cTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                                                                               >  2:30224/1‑150 (MQ=255)
                                          cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCTTAACGACGCaa                                                                                                             >  2:1306572/1‑150 (MQ=255)
                                          cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACTACGCaa                                                                                                             >  1:1097624/1‑150 (MQ=255)
                                          cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                                                                             >  2:718750/1‑150 (MQ=255)
                                          cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                                                                             >  2:1152140/1‑150 (MQ=255)
                                           aaaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAt                                                                                                            >  1:1125734/1‑150 (MQ=255)
                                            aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAtg                                                                                                           >  2:949700/1‑150 (MQ=255)
                                              aTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCCGCCTTGTTTCATGGCCGGAGGACAAAATAAAATCTGCTTAGCATAACGACGCAAtgtg                                                                                                         >  2:1344928/1‑150 (MQ=255)
                                               tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                        <  2:675350/150‑1 (MQ=255)
                                               tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                        <  2:184538/150‑1 (MQ=255)
                                               tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                        <  1:1390927/150‑1 (MQ=255)
                                                    tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGacaca                                                                                                   <  2:1780679/150‑1 (MQ=255)
                                                     gCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCAGAACGCCGCAATGTGGacaccc                                                                                                  >  1:931883/1‑148 (MQ=255)
                                                       aaaaGTAATGAGTCATTTTTCTTGATTATAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGGCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACtt                                                                                                >  1:64631/1‑150 (MQ=255)
                                                         aaGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                                                                              <  1:392746/150‑1 (MQ=255)
                                                                      tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTg                                                                                 <  2:383606/150‑1 (MQ=255)
                                                                      tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTAGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTg                                                                                 <  1:1331881/150‑1 (MQ=255)
                                                                        tttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTa                                                                               <  1:1745799/150‑1 (MQ=255)
                                                                              gATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                        <  2:1750467/150‑1 (MQ=255)
                                                                               aTTACAGGTGTTACCAACTCCCCAACTACCTCGGCGTCCTCACGCACTCGCCATAACCGGCATTGGTTCATGGCAGTTGGCGAAAATAAATTCTGCTTAGAAGACCTCCGCATTGTGGCCCCAGGTGGAGGATGACTTCTGTATTTTTcc                                                                       >  1:299480/1‑149 (MQ=255)
                                                                                ttACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                                                                      <  1:412768/150‑1 (MQ=255)
                                                                                 tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                     <  2:1392131/150‑1 (MQ=255)
                                                                                 tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTGTCATGGCCGGTGGACATAATAAATGCTGCTTAGCATAACGCCGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                     >  1:328378/1‑150 (MQ=255)
                                                                                   cAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTATCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGAAGCACTGGGAGGATTACTTCTCTATTTTTcagcat                                                                   >  1:316600/1‑149 (MQ=255)
                                                                                           aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttct                                                           >  2:554928/1‑150 (MQ=255)
                                                                                            ccAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttctt                                                          >  1:1259933/1‑150 (MQ=255)
                                                                                             cAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttcttc                                                         >  2:1527675/1‑150 (MQ=255)
                                                                                               aCTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCaa                                                       <  1:982894/150‑1 (MQ=255)
                                                                                                cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                      <  2:803109/150‑1 (MQ=255)
                                                                                                  ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCTCATTCTTCAagag                                                    >  2:911839/1‑150 (MQ=255)
                                                                                                  ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAagag                                                    >  1:1530213/1‑150 (MQ=255)
                                                                                                  ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGTGTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTTTTCAagag                                                    >  1:1277766/1‑150 (MQ=255)
                                                                                                    ccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTCGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGTGtt                                                  >  1:159872/1‑150 (MQ=255)
                                                                                                     cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTa                                                 >  1:979175/1‑150 (MQ=255)
                                                                                                      aactaactCGGCGTCCTCACGCTCTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                        <  2:766673/126‑1 (MQ=255)
                                                                                                      aactaactCGGCGTCCTCACGCGCTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                        >  1:766673/1‑126 (MQ=255)
                                                                                                       actaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAAc                                               <  1:949700/150‑1 (MQ=255)
                                                                                                          aactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATATCGACGCAATGTGGCGACACTTGGAGGAGTACTTCTGTATTTTTCAGCAGCCCGTTCTTCAAGAGTTAACAtt                                            >  2:901004/1‑150 (MQ=255)
                                                                                                          aactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACAtt                                            >  2:197092/1‑150 (MQ=255)
                                                                                                            ctCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTg                                          <  1:1176142/150‑1 (MQ=255)
                                                                                                            ctCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTg                                          <  1:1782932/150‑1 (MQ=255)
                                                                                                             tCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGa                                         >  2:332963/1‑150 (MQ=255)
                                                                                                             tCGGAGTCCTCACGAACTAGCCATAACCGGCCTTGTTTCATGTCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGa                                         <  1:633139/150‑1 (MQ=255)
                                                                                                               ggCGTCCTCACGCACTCGACATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAg                                       <  2:1185337/150‑1 (MQ=255)
                                                                                                                gCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACCCTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGc                                      >  2:1445725/1‑150 (MQ=255)
                                                                                                                     cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                                 >  2:1069453/1‑150 (MQ=255)
                                                                                                                          ggtcTCGCCAAAACCGGCCTTGTTTTATGGCCGGTGTACAAAATAAATTCTGTTTAGCATAACGACGCAATGTGGACACACTTGGTTGAATACTTCTTTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATc                            <  2:182770/147‑1 (MQ=255)
                                                                                                                          gCACTCGCCATAACCCGCCTTGTTTCATGGCCTGTGGACAAAATAAATTCTGCTTAGCATAACGACTCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCAATTCTTCAAGAGTTAACATTTGAAGCATATGCGATc                            <  2:79324/150‑1 (MQ=255)
                                                                                                                             cTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                         <  2:1467457/150‑1 (MQ=255)
                                                                                                                             cTCGCCATAACATGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                         <  1:1101943/150‑1 (MQ=255)
                                                                                                                              tCGCCATAACCGGACTTGTTTCATGGCCGGTGGACAAAAAAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCt                        <  1:311952/150‑1 (MQ=255)
                                                                                                                                  gtccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                    >  2:1680933/5‑150 (MQ=255)
                                                                                                                                     caCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtact                 >  2:495888/2‑150 (MQ=255)
                                                                                                                                     caCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAGTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAAGATTTGAAGCATATGCGATCACCTTATtact                 >  2:302141/2‑150 (MQ=255)
                                                                                                                                      aCCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTATCATAACGAAGCAATGTGGACACACTTGGAGGATTACTTCTGTATATTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                <  2:1561022/150‑1 (MQ=255)
                                                                                                                                       ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac               <  2:57567/150‑1 (MQ=255)
                                                                                                                                        cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTaca              >  1:1446627/1‑150 (MQ=255)
                                                                                                                                         ggCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTTTTTTTTTAATGATATGGGTTCAACTTATTTCTacac             <  2:856637/150‑1 (MQ=255)
                                                                                                                                         ggCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGAGATCACCTTATTACTacaa             >  1:1146676/1‑149 (MQ=255)
                                                                                                                                         ggCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACATCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTacac             <  1:852778/150‑1 (MQ=255)
                                                                                                                                          gCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACt            >  1:506665/1‑150 (MQ=255)
                                                                                                                                            cTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCATAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTaa          <  1:203244/150‑1 (MQ=255)
                                                                                                                                               gTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTg       >  2:1619181/1‑150 (MQ=255)
                                                                                                                                                 ttCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCt     <  1:1692615/150‑1 (MQ=255)
                                                                                                                                                    aTGGCCGGTGGACAAAATATATTATGCTTCGCATAACGACGCAATGTGGATATACTTGGAGGATTACTTCTTTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAATGATATGCGATCACCTTATTACTACACTAACTGCTTAg  <  2:316600/150‑1 (MQ=255)
                                                                                                                                                    aTGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAg  >  1:407165/1‑150 (MQ=255)
                                                                                                                                                     tGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAg  >  2:134463/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAG  >  NC_000913/1200769‑1201066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: