Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,173 C→T 5.3% L185L (CTG→TTG)  accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,1730CT 5.3% 403.0 / 6.8 131L185L (CTG→TTG) accAacetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (67/56);  new base T (4/3);  total (71/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 136 total aligned reads displayed.

CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGCG  >  NC_000913/209024‑209322
                                                                                                                                                     |                                                                                                                                                     
cAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGCACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAccc                                                                                                                                                       >  1:227280/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                     >  1:965456/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTATAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                     >  2:1088898/1‑150 (MQ=255)
   cgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGc                                                                                                                                                    <  2:830375/150‑1 (MQ=255)
    gcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCATATCCTGGAGTTGGCGCAGAAGCGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg                                                                                                                                                   >  2:1007206/1‑150 (MQ=255)
    gcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg                                                                                                                                                   >  1:1277391/1‑150 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACCCCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaa                                                                                                                                               >  1:1288027/1‑150 (MQ=255)
           ggTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAGGTTGCGTGCAATg                                                                                                                                            <  1:818674/150‑1 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                           <  1:631809/150‑2 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                           <  1:1254893/150‑2 (MQ=255)
             ttACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtc                                                                                                                                          >  1:1345569/1‑150 (MQ=255)
               aCCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACCCCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtctc                                                                                                                                        >  1:1572748/1‑150 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                       >  1:805266/1‑150 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                       >  2:664128/1‑150 (MQ=255)
                 cGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTAACGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGc                                                                                                                                      >  2:826921/1‑150 (MQ=255)
                            gCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTAcc                                                                                                                           >  1:1278710/1‑150 (MQ=255)
                               tCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCACCCTGCGTGAAATGTCACGCCTCGGCGTCCCGGt                                                                                                                        >  1:1635119/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                       >  2:1479993/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCCACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                       >  2:955803/1‑150 (MQ=255)
                                  gATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGt                                                                                                                     >  2:1478684/1‑150 (MQ=255)
                                    tGCTAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGttt                                                                                                                   >  1:942114/1‑150 (MQ=255)
                                    tGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGttt                                                                                                                   >  2:1665533/1‑150 (MQ=255)
                                     gCAAATGGCTGAAGGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTg                                                                                                                  <  2:1431594/150‑1 (MQ=255)
                                     gCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTg                                                                                                                  <  2:1345569/150‑1 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGt                                                                                                                 >  1:1019936/1‑150 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCAATCATCACATTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGAGGTCTGTCTGAAGCCATTGCACGCAACCTGCGAGAAATGTCTCGCCTCGGCGTACCGGGAGTTAGt                                                                                                                 >  1:871580/1‑150 (MQ=255)
                                               gAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCC‑GGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                       >  1:1773431/1‑150 (MQ=255)
                                                aaCGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                       <  1:72748/150‑1 (MQ=255)
                                                      ttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAg                                                                                                 <  2:1800742/150‑1 (MQ=255)
                                                       tAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAgg                                                                                                <  1:1665533/150‑1 (MQ=255)
                                                             gCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTc                                                                                          >  2:1362992/1‑150 (MQ=255)
                                                              ccTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTATGTACGGTTAGCGGTGAAGGTGGTTCt                                                                                         >  2:1344860/1‑150 (MQ=255)
                                                               cTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTg                                                                                        >  2:171920/1‑150 (MQ=255)
                                                                  tcatcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGTGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCg                                                                                     <  1:1592955/150‑1 (MQ=255)
                                                                  tcatcaCCTTTAGCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTGCTGGCg                                                                                     <  2:1394576/150‑1 (MQ=255)
                                                                   catcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCgg                                                                                    <  1:1774277/150‑1 (MQ=255)
                                                                    atcaCCTATATCGACAGCCCCGGGGCTT‑TCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCTACCTGCGTGATATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                  <  2:685008/150‑1 (MQ=255)
                                                                     tcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                  >  1:1039723/1‑150 (MQ=255)
                                                                     tcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                  <  1:664128/150‑1 (MQ=255)
                                                                      caCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTTTCGGTGAAGGTGGTTCTGGCGGTgc                                                                                 >  1:224819/1‑150 (MQ=255)
                                                                       aCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGGCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcg                                                                                <  2:1447773/150‑1 (MQ=255)
                                                                       aCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGGGGGCAGTCTGAAGCCATTGCACGCACCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcg                                                                                <  2:1198962/150‑1 (MQ=255)
                                                                        ccTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcgc                                                                               >  2:855102/1‑150 (MQ=255)
                                                                          tttATCGTCACCCCGGGGGCTTATGCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGTCGGTGCGCTg                                                                             <  1:505655/150‑1 (MQ=255)
                                                                             aTCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACTAAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTCGTTCTGGCGGTGCGCTGGCg                                                                          <  2:1764973/150‑1 (MQ=255)
                                                                               cGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGAt                                                                        >  1:127396/1‑150 (MQ=255)
                                                                                gACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                       >  1:378302/1‑150 (MQ=255)
                                                                                gACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                       >  1:885115/1‑150 (MQ=255)
                                                                                  cacCCCGGGTGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                     <  2:849020/150‑1 (MQ=255)
                                                                                  cacCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                     >  2:22344/1‑150 (MQ=255)
                                                                                  cacCCCGGGGGCGTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                     <  1:1102531/150‑1 (MQ=255)
                                                                                   cccccGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGc                                                                    <  1:321424/149‑1 (MQ=255)
                                                                                    ccccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                   <  2:1621444/150‑1 (MQ=255)
                                                                                      ccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTg                                                                 <  1:1171809/150‑1 (MQ=255)
                                                                                         ggggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGc                                                              <  2:652057/150‑1 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTTAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                             <  2:955940/150‑1 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                             >  2:372884/1‑150 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                             <  1:1780161/150‑1 (MQ=255)
                                                                                           ggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGa                                                            >  1:1001762/1‑150 (MQ=255)
                                                                                               tATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaaa                                                        >  2:585465/1‑150 (MQ=255)
                                                                                                aTCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAg                                                       >  1:777956/1‑150 (MQ=255)
                                                                                                 tCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                                                      <  1:826921/150‑1 (MQ=255)
                                                                                                 tCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                                                      <  2:1454476/150‑1 (MQ=255)
                                                                                                  ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTg                                                     >  2:1720216/1‑150 (MQ=255)
                                                                                                   cTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGa                                                    >  2:1015095/1‑150 (MQ=255)
                                                                                                   cTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGa                                                    >  1:1464314/1‑150 (MQ=255)
                                                                                                          gggCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgct                                             <  1:1448549/150‑1 (MQ=255)
                                                                                                           ggCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctg                                            >  1:519679/1‑150 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCGACCTGCGTGAATTGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGGGATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGGGATTGGCGTGGGCGAGAAAGTGAATAtgctgc                                           >  1:76220/1‑150 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctgc                                           <  2:745284/150‑1 (MQ=255)
                                                                                                             cgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCa                                          >  2:1201484/1‑150 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                         <  1:733731/150‑1 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                         >  2:424580/1‑150 (MQ=255)
                                                                                                               cAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAATTGAATATGCTGCAAt                                        >  2:1814589/1‑150 (MQ=255)
                                                                                                                 gaagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATAc                                      >  1:303261/1‑150 (MQ=255)
                                                                                                                  aagaGCTTGCTCAGTCTGAAGCCATTGCATTCAAGATGCGTGAAATGTCTCTCATAGTCGTACCGGTAGTTTGTAAGGTTATCGGTGAAGGTTTTTCTGTCGGTGCTCTTGCGATTGGCGTGGGCGATAAAGTTAATATGCTGCAATACa                                     <  1:286377/150‑1 (MQ=255)
                                                                                                                   agagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAg                                    <  2:1759218/150‑1 (MQ=255)
                                                                                                                   agagCGGGGGCAGGCGGTAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGGTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAg                                    <  1:1119017/150‑1 (MQ=255)
                                                                                                                      gCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCAc                                 >  1:231610/1‑150 (MQ=255)
                                                                                                                        gTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCt                               >  1:1049009/1‑150 (MQ=255)
                                                                                                                          ggTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAGAAAGTGAATATGCTGCAATACAGCAACTAt                             >  1:1473275/1‑150 (MQ=255)
                                                                                                                             gaGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCTGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCTCCTATTcc                          <  2:883013/149‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                        >  2:652606/1‑150 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                        >  1:288030/1‑150 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                        <  1:1478684/150‑1 (MQ=255)
                                                                                                                                 cTGAAGCCAGTGCACGCAACCTGCGGGAAATGTCCCGACTCCGCGTACCGGTAGTTTTTTCTGTTATCTGTGAAGGTGGTTCTGGCGTTGCGCTGGCGATTGGCGTGGGCGATTAAGTGAATATGCTGGAATACAGCACCTATTCCGTTa                      <  2:273728/150‑1 (MQ=255)
                                                                                                                                  tGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                     >  1:1213308/1‑150 (MQ=255)
                                                                                                                                  gcgagacATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTTGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                     >  2:68024/7‑150 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtc                    >  1:393501/1‑150 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAta                    >  2:386198/1‑149 (MQ=255)
                                                                                                                                     aGCCATTGGACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtctc                  <  1:11960/150‑1 (MQ=255)
                                                                                                                                      gacATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCAGTTATCTCg                 >  1:1568317/3‑150 (MQ=255)
                                                                                                                                      gCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCg                 >  2:673162/1‑150 (MQ=255)
                                                                                                                                        cATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc               >  1:695450/1‑150 (MQ=255)
                                                                                                                                        cATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc               <  2:1288027/150‑1 (MQ=255)
                                                                                                                                             cACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACCGCACCTATTCCGTTATCTCGCCGGAAg          >  1:1001353/1‑150 (MQ=255)
                                                                                                                                                   agcTGCGTGAAATGTCTCGCCTCGTCGTACCGTTAGTTTGTTCGGTGTTCGGTGAATGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTtgtg    <  2:82354/148‑1 (MQ=255)
                                                                                                                                                   aCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTTGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTtgtg    <  2:1196014/150‑1 (MQ=255)
                                                                                                                                                   aCCTGCGGGAAATGTCTCGCCGCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCATCTATTCCGTTATCTCGCCGGAAGGTtgtg    <  2:1533393/150‑1 (MQ=255)
                                                                                                                                                    ccTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGc   <  2:1608300/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGCG  >  NC_000913/209024‑209322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑