Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,157 T→A 5.1% intergenic (‑127/+19) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1570TA 5.1% 291.5 / 4.6 98intergenic (‑127/+19)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (45/48);  new base A (3/2);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCAT  >  NC_000913/1233009‑1233306
                                                                                                                                                    |                                                                                                                                                     
aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTtata                                                                                                                                                      <  1:1331574/150‑1 (MQ=255)
  tGGTTAAAAAATCGCAACCTACTCATTACCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATg                                                                                                                                                    <  2:1130926/150‑1 (MQ=255)
    gTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGcc                                                                                                                                                  >  1:963595/1‑150 (MQ=255)
      tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCg                                                                                                                                                <  2:26791/150‑1 (MQ=255)
      tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCg                                                                                                                                                >  2:1614820/1‑150 (MQ=255)
       cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCgg                                                                                                                                               <  1:182407/150‑1 (MQ=255)
       cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCACCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACTGGGAATTTATATGCACgg                                                                                                                                               >  2:94626/1‑150 (MQ=255)
           aaTCGCAACATAATCATTTCCCTGCAATATGCTTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAg                                                                                                                                           <  2:259210/150‑1 (MQ=255)
           aaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttg                                                                                                                                           <  2:1722681/150‑4 (MQ=255)
            aTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgt                                                                                                                                          <  1:70060/150‑1 (MQ=255)
              cGCAACATACTCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                                                        <  1:1122119/150‑1 (MQ=255)
              cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                                                        >  2:410450/1‑150 (MQ=255)
                 aaCATAATCATTTCCCTGCAATATTCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa                                                                                                                                     <  1:903694/150‑1 (MQ=255)
                  aCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTAt                                                                                                                                    <  2:1426472/150‑1 (MQ=255)
                     tAATCATTTCCCTGCAATATGCATAGAGTGCGCAGGTTAAACCAATTCATTCGCTGCGCCATCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCCGTAAGTTGTCATCaa                                                                                                                                 >  1:1128416/1‑150 (MQ=255)
                      aaTCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCaaa                                                                                                                                >  2:1066145/1‑150 (MQ=255)
                        tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                              >  2:808521/1‑150 (MQ=255)
                         cATTTCCCTGCAATATGCATAGAGTGCTCAGTTTAAACCAATTCAATCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAATTATATGCCCGGTAAGTTGTTATCAAAGCg                                                                                                                             <  1:1400343/150‑1 (MQ=255)
                         cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGTGCGAAATGGCAAACCGGGCATTTATATGCGCGGTAAGTTGTTATCAAAGct                                                                                                                             >  2:275616/1‑149 (MQ=255)
                         cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCg                                                                                                                             <  2:1261058/150‑1 (MQ=255)
                            ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg                                                                                                                          >  1:1190717/1‑150 (MQ=255)
                            ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCAACCAGGAGGCAGCAAAAATAGGACTCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg                                                                                                                          <  1:1659413/150‑1 (MQ=255)
                             tCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGc                                                                                                                         <  2:1673367/150‑1 (MQ=255)
                               ccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTa                                                                                                                       >  1:802199/1‑150 (MQ=255)
                                cTGCAATATGCATAGAGTGCGCAGTTTAAAACAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGATGTTATCAAAGCGTTGCTAt                                                                                                                      >  1:283613/1‑150 (MQ=255)
                                cTGCAATATGCATAGAGTGCGCAGGTTAAACCAATTCATTCGCTGCGCCACCAGGCGGCAGCAAAAATAGGACGCAATTGGACAATTAGCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGGTGCTAt                                                                                                                      <  1:1140085/150‑1 (MQ=255)
                                   cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATccc                                                                                                                   >  2:1293233/1‑149 (MQ=255)
                                     atatGCATAGAGTGCTCATTTTAAACAAATTCATTCGCTGCTCCAACAGGAGGCATCAAAAATAGGACGCAATTGGACAATTATCGTGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGc                                                                                                                 <  2:907737/150‑1 (MQ=255)
                                            tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                          <  2:776287/150‑1 (MQ=255)
                                            tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                          <  1:1174621/150‑1 (MQ=255)
                                            tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                          <  2:479954/150‑1 (MQ=255)
                                             cgagTGAGCAGTTTACACCAATTCATTCGCGGCGCCACCAGGCGGCAGCAAAAATAGGCCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTGGCTATCCAGCCCATTTGc                                                                                                         <  2:864947/149‑1 (MQ=255)
                                             agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                                         >  2:397429/1‑150 (MQ=255)
                                               agTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAt                                                                                                       <  1:357496/150‑1 (MQ=255)
                                                gTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATa                                                                                                      >  2:694170/1‑150 (MQ=255)
                                                 tgtgaattttAAACCAATTCATTCGCTGCGCCACCATGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATACAGCCCATTTGCATaa                                                                                                     <  2:1155465/143‑1 (MQ=255)
                                                 tGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa                                                                                                     >  1:214422/1‑150 (MQ=255)
                                                  gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCCTTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                    >  1:1391917/1‑150 (MQ=255)
                                                  gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                    <  1:708631/150‑1 (MQ=255)
                                                  gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                    >  1:1519337/1‑150 (MQ=255)
                                                   cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                                                   >  2:1131772/1‑150 (MQ=255)
                                                           aaaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTAATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTCTCCAGCCCATTTGCATAAACCATTCGGt                                                                                           >  2:676958/1‑150 (MQ=255)
                                                            aaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                                          >  1:1239799/1‑150 (MQ=255)
                                                            aaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                                          <  2:1519475/150‑1 (MQ=255)
                                                                 attcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgg                                                                                     >  2:1185181/1‑150 (MQ=255)
                                                                 attcattcGCAGCTCCACGAGCAGGCAGCAAAAATAGTACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgg                                                                                     <  1:95249/150‑1 (MQ=255)
                                                                   tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGTACAATTATCGTGCGAAAAGGCCAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                                                   <  1:892714/150‑1 (MQ=255)
                                                                     attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                                                 <  2:1754547/150‑1 (MQ=255)
                                                                         gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                             <  2:62522/150‑1 (MQ=255)
                                                                         ccTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTAACGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                             <  2:181951/149‑1 (MQ=255)
                                                                             cgcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaaa                                                                         >  1:501064/1‑150 (MQ=255)
                                                                               ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                                                       <  2:354755/150‑1 (MQ=255)
                                                                               ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGAGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                                                       >  1:603461/1‑150 (MQ=255)
                                                                                caccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                                                      >  1:1711464/1‑150 (MQ=255)
                                                                                 accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCg                                                                     <  1:1686646/150‑1 (MQ=255)
                                                                                  ccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa                                                                    >  1:1354528/1‑150 (MQ=255)
                                                                                        ggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATTTGCCCGGTAAGTTGTTATCTAAGCGTTGCTATCCAGCCAATTTGCATAAACCATTCGGTTTCAGGGGCAAGCGTAAACTCGACGCaga                                                              >  1:299650/1‑150 (MQ=255)
                                                                                        ggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTATACGCaga                                                              >  1:1141968/1‑150 (MQ=255)
                                                                                            gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCTTTCGCTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                          >  2:1668405/1‑150 (MQ=255)
                                                                                            gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCACGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                          >  1:233394/1‑150 (MQ=255)
                                                                                             caAAAATAGGTCGCAATTGGACAATTATCGGGCGAATAGGGATACCGGGGATTAATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTTCATAAACCATTCGGATACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                         <  2:1734994/150‑1 (MQ=255)
                                                                                             aaaaaaTAGGACGCAATTGGACAATTATAGGGCGAAAAGGCAAACCGGGCATTTTTTTGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCGAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                         <  1:709790/149‑1 (MQ=255)
                                                                                               aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGACAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                                                       >  2:1577139/1‑150 (MQ=255)
                                                                                                       aCGCAATTGGACAATTATCGGGCGAATAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGtgtg                                               <  2:1687477/150‑1 (MQ=255)
                                                                                                          aaaTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCa                                            <  1:625884/149‑1 (MQ=255)
                                                                                                             ttGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGa                                         <  1:1552637/150‑1 (MQ=255)
                                                                                                               ggACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACg                                       >  1:1803522/1‑150 (MQ=255)
                                                                                                                gACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGa                                      >  2:1350413/1‑150 (MQ=255)
                                                                                                                 aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                                     <  1:260404/150‑1 (MQ=255)
                                                                                                                   aaTTATCGGGCGAAAAGGCAAACCTGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCATGCGTTAACTCGACGCAGAGCAAGCCGACGAGTGTCAGTACGAggg                                   <  2:3516/150‑1 (MQ=255)
                                                                                                                   aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAggg                                   <  1:397837/150‑1 (MQ=255)
                                                                                                                   aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAggg                                   <  2:963595/150‑1 (MQ=255)
                                                                                                                      tATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAATCGTTGCTATCCAGCACATTTGCATAAACCAATCTGTTAAAGGGGCACGCGTAAACAAGACGCAGAGCAAGCGTACGAGTGACTGTACGAGGgtgt                                >  2:294453/1‑150 (MQ=255)
                                                                                                                      tATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAACCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgtgt                                >  1:1126385/1‑150 (MQ=255)
                                                                                                                       aTCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTa                               <  1:812052/150‑1 (MQ=255)
                                                                                                                           ggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGc                           >  1:932896/1‑150 (MQ=255)
                                                                                                                            gCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTATAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCa                          <  1:488989/150‑1 (MQ=255)
                                                                                                                             cGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                         <  2:214422/150‑1 (MQ=255)
                                                                                                                             cGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                         >  1:1429728/1‑150 (MQ=255)
                                                                                                                                aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc                      <  1:410450/150‑1 (MQ=255)
                                                                                                                                aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc                      <  2:1190717/150‑1 (MQ=255)
                                                                                                                                  aGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCa                    >  1:1756303/1‑150 (MQ=255)
                                                                                                                                    gCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTAGACGCAGAGCAAGACGACTAGAGACACGAAGATGGTGTAAGGCAGGGCCATc                  >  1:1452319/1‑150 (MQ=255)
                                                                                                                                     cttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                 >  2:1630314/4‑150 (MQ=255)
                                                                                                                                     cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                 <  1:1378287/150‑1 (MQ=255)
                                                                                                                                     cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                 <  2:802199/150‑1 (MQ=255)
                                                                                                                                     cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                 >  2:1254707/1‑150 (MQ=255)
                                                                                                                                      aaaCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCca                >  2:1089354/1‑150 (MQ=255)
                                                                                                                                       taCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGCCGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcac               >  2:1726409/2‑150 (MQ=255)
                                                                                                                                       aaCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcac               >  1:550783/1‑150 (MQ=255)
                                                                                                                                        aCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcaca              >  2:1359020/1‑150 (MQ=255)
                                                                                                                                         ccGGGCATTTATATGCCCGGTAAGTTGTTCTCAAAGCGTCGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCacac             <  1:1136095/150‑1 (MQ=255)
                                                                                                                                         ccGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCacac             >  1:786792/1‑150 (MQ=255)
                                                                                                                                         ccGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCacac             >  1:1074304/1‑150 (MQ=255)
                                                                                                                                         ccGGGAATTTATATGCCCGGTAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATTTGCAGAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCacac             <  2:1163057/150‑1 (MQ=255)
                                                                                                                                            ggCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACCCCAt          >  2:1145806/1‑150 (MQ=255)
                                                                                                                                                tttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCgg      >  1:792171/1‑150 (MQ=255)
                                                                                                                                                 ttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGc     <  2:1803745/150‑1 (MQ=255)
                                                                                                                                                 ttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGc     <  2:917433/150‑1 (MQ=255)
                                                                                                                                                    tatGACCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCAt  >  1:426618/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCAT  >  NC_000913/1233009‑1233306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: