Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,346,957 G→T 5.4% intergenic (+57/+21) ymiB → / ← rnb uncharacterized protein/ribonuclease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,346,9570GT 5.4% 337.1 / 5.7 113intergenic (+57/+21)ymiB/rnbuncharacterized protein/ribonuclease II
Reads supporting (aligned to +/- strand):  ref base G (44/62);  new base T (4/3);  total (48/65)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 116 total aligned reads displayed.

AAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCA  >  NC_000913/1346808‑1347106
                                                                                                                                                     |                                                                                                                                                     
aaaTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAg                                                                                                                                                       <  1:722745/150‑1 (MQ=255)
 aaTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAgg                                                                                                                                                      <  1:1081345/150‑1 (MQ=255)
     aaCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATg                                                                                                                                                  >  2:1572350/1‑150 (MQ=255)
      aCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgg                                                                                                                                                 >  2:1746287/1‑150 (MQ=255)
      aCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgg                                                                                                                                                 <  1:1156506/150‑1 (MQ=255)
      aCGGCGGCTAAACGCATTGTATTTTTTATATAATTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAAGGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgt                                                                                                                                                 >  2:263356/1‑149 (MQ=255)
        ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGc                                                                                                                                               >  2:1708260/1‑150 (MQ=255)
        ggcggcTAAACGCATTGTATTTTTTATATATTTAATTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGTGAAATAAACGGCCCATTCCTCATGGATGGGc                                                                                                                                               <  1:347804/150‑1 (MQ=255)
         gcggaTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGcc                                                                                                                                              <  2:1635355/150‑1 (MQ=255)
          cggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCg                                                                                                                                             <  1:1537157/150‑1 (MQ=255)
             cTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGttt                                                                                                                                          <  2:1564047/150‑3 (MQ=255)
             cTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGa                                                                                                                                          >  2:402132/1‑150 (MQ=255)
                aaCTCATGGTTATTATTATATATTTCTTAGAAATCCATTTTTGCAAACACGCCAGTTTTCAAACGAATCTCAGATAAAAAATCTGCCTGGATATAACGAAGGTAGAGCTGGCAACTAAACTGCCCATCCATTAGTAATGGGCCGTGAAAg                                                                                                                                       <  1:302995/150‑1 (MQ=255)
                aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAg                                                                                                                                       <  1:832811/150‑1 (MQ=255)
                 aCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGACCATCCATGAGGAATGGGCCGTGAAAgg                                                                                                                                      >  1:820893/1‑150 (MQ=255)
                  cGCATTGTTTTTTTTTTATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGga                                                                                                                                     <  2:369667/150‑1 (MQ=255)
                  cGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGAAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATCGGCCGTGAAAGga                                                                                                                                     >  2:728302/1‑150 (MQ=255)
                   gctttGTTTTTTTTATATATTTATTTGTAATACAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                    <  1:169796/147‑1 (MQ=255)
                     aTTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGAt                                                                                                                                  <  2:1693547/150‑1 (MQ=255)
                             ttttATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGAc                                                                                                                          <  1:1454321/150‑1 (MQ=255)
                              tttATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTCAAAAAATCTGCCTGGACATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGAcc                                                                                                                         >  1:1663898/1‑150 (MQ=255)
                                  tatatTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgc                                                                                                                     >  1:762616/1‑150 (MQ=255)
                                    tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCATGGATATAACGAAGGTAGAGCGGGGAAATAAACGGACCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                   >  1:106411/1‑150 (MQ=255)
                                     atttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgcg                                                                                                                  >  2:1696711/1‑150 (MQ=255)
                                     atttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgcg                                                                                                                  >  2:207792/1‑150 (MQ=255)
                                        tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGATATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaa                                                                                                               >  2:602662/1‑150 (MQ=255)
                                         atttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaat                                                                                                              >  2:1180786/1‑150 (MQ=255)
                                           ttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataa                                                                                                            >  1:935740/1‑150 (MQ=255)
                                              tAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgc                                                                                                         <  2:238072/150‑1 (MQ=255)
                                               aaTCCAGTTTTGGAAAAACGGCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGACGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgct                                                                                                        <  2:107569/150‑1 (MQ=255)
                                               aaTCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGTTATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgct                                                                                                        <  2:102882/150‑1 (MQ=255)
                                               aaTCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGATGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgct                                                                                                        >  2:1143245/1‑150 (MQ=255)
                                               aaTACAGTTTTGGAAAAAGTCCAGTTTTAAAACTAAAGTCAGTTAAAATATCTGCCTGGATATAACGATGGTAGAGCGGGTAAATAAACGGCCCATCCATTAGTAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgct                                                                                                        <  2:1358018/150‑1 (MQ=255)
                                                   cAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCgg                                                                                                    <  2:9848/150‑1 (MQ=255)
                                                   cAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCgg                                                                                                    >  1:598528/1‑150 (MQ=255)
                                                    tgTTTTGTAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAACATCTGCCTGTATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGACTGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCggg                                                                                                   <  1:157547/149‑1 (MQ=255)
                                                    aGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCggg                                                                                                   <  2:1050414/150‑1 (MQ=255)
                                                       tttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGttt                                                                                                >  1:705976/1‑150 (MQ=255)
                                                        ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                               <  1:818524/150‑1 (MQ=255)
                                                           gAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCAGTGAAAGGAGATTAAGCGACCGTGCGCGCAATAATGCTGCGTGTTTCCAt                                                                                            >  1:238721/1‑150 (MQ=255)
                                                             aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGATCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                          <  2:1016629/150‑1 (MQ=255)
                                                             aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                          <  1:470087/150‑1 (MQ=255)
                                                             aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGGAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                          <  2:238628/150‑1 (MQ=255)
                                                              aaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCg                                                                                         <  2:1032284/150‑1 (MQ=255)
                                                                 cGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGAc                                                                                      <  2:505422/150‑1 (MQ=255)
                                                                  gCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACt                                                                                     >  2:1072019/1‑150 (MQ=255)
                                                                   ccAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACtt                                                                                    >  2:951228/1‑150 (MQ=255)
                                                                    cAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTc                                                                                   <  1:978755/150‑1 (MQ=255)
                                                                     aGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCg                                                                                  <  1:989290/150‑1 (MQ=255)
                                                                       ttttCAAACTAATGTCAGTTAAAAAATCTTCCTGGATATAAACAAGGTAGAGCGGGTAAATAAACGGCCCATCCATGAGTATTGGGCCGTTAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                <  2:1523912/150‑1 (MQ=255)
                                                                       ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                <  1:527058/150‑1 (MQ=255)
                                                                           cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                            >  2:228346/1‑150 (MQ=255)
                                                                           aaaaCGAAAGTGAGTTAAAAAATCTGCGTGGATTTAACGAAGGTAGAGCGGGGAAAAAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAGGCTACCTGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                            <  1:318814/149‑1 (MQ=255)
                                                                            aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGGAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                           >  2:1304962/1‑150 (MQ=255)
                                                                            aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                           >  2:714868/1‑150 (MQ=255)
                                                                            aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCACCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                           >  2:226470/1‑150 (MQ=255)
                                                                                   aGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTc                                                                    <  1:622535/150‑1 (MQ=255)
                                                                                   aGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTc                                                                    <  1:17819/150‑1 (MQ=255)
                                                                                          tAAAAAATCTGCCTTGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                             <  2:26585/150‑1 (MQ=255)
                                                                                          tAAAAAATCTGCCTGGATATACCGAAGGTAGAGCGGGGAAATAAACGGCCCCACCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                             <  2:1143387/150‑1 (MQ=255)
                                                                                          tAAAAAATCTGCCTGGATATAACGAAGGTTGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                             >  2:1416582/1‑150 (MQ=255)
                                                                                          tAAAAAATATGCCTGGATATAACGAAGGTAGAGCTGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCTGGCGCGCAATAATGCTGCGGGTTTCAATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                             >  1:1720110/1‑150 (MQ=255)
                                                                                           aaaaaaTCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgt                                                            >  1:364037/1‑150 (MQ=255)
                                                                                            aaaaaTCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgtc                                                           >  1:1007278/1‑150 (MQ=255)
                                                                                                     ccTGGATATAACGAAGGTAGAGCGGGGAAATAAACGTCCCATCCATGAGGAATGGGCCGTGAAAGGATATTACGCGACCGGGCGCGCAATAATGCTGCGTGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACttt                                                  <  1:992031/150‑1 (MQ=255)
                                                                                                     ccTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACttt                                                  >  1:1433126/1‑150 (MQ=255)
                                                                                                       tGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAATGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGt                                                <  2:890946/150‑1 (MQ=255)
                                                                                                       tGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGt                                                <  2:847587/150‑1 (MQ=255)
                                                                                                            ataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGATATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAcc                                           <  1:1572350/150‑1 (MQ=255)
                                                                                                            ataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAcc                                           <  2:676826/150‑1 (MQ=255)
                                                                                                             taACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCg                                          >  2:1737994/1‑150 (MQ=255)
                                                                                                                cGAAGGTAGAGCGGGGAAATAACCGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGttt                                       <  1:635591/150‑1 (MQ=255)
                                                                                                                 gAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                      >  1:1007990/1‑150 (MQ=255)
                                                                                                                 caaGATAGTGCGGGTAAAAAAACGGCCCATCCATGATGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                      <  2:650824/149‑1 (MQ=255)
                                                                                                                   aGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAc                                    <  1:1746287/150‑1 (MQ=255)
                                                                                                                    ggTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   <  2:881909/150‑1 (MQ=255)
                                                                                                                    ggTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   >  2:59801/1‑150 (MQ=255)
                                                                                                                    ggTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   <  1:468124/150‑1 (MQ=255)
                                                                                                                    ggTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   >  2:1479529/1‑150 (MQ=255)
                                                                                                                    ggTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGATGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   >  2:1022072/1‑150 (MQ=255)
                                                                                                                       agagCGGGGAAATAAACGGCCCACCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGTCGTCATTAACGTCAGTCACTTTGTAAACCGTTTCACCttt                                <  1:226470/150‑1 (MQ=255)
                                                                                                                        gagCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTa                               <  1:668677/150‑1 (MQ=255)
                                                                                                                         agCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTaa                              >  1:150971/1‑150 (MQ=255)
                                                                                                                             gggAAATAAACGGCCCATCCATGAGGATTGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTg                          >  2:1314057/1‑150 (MQ=255)
                                                                                                                             gggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTg                          <  1:1122567/150‑1 (MQ=255)
                                                                                                                              ggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGt                         <  1:209285/150‑1 (MQ=255)
                                                                                                                               gAAATAAACGGCCCAACCATGAGGAATGGGACGTGAAAGGAGATAACGCGACCTGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCTGCAATAGTGACGTGAAAAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTa                        <  1:263356/150‑1 (MQ=255)
                                                                                                                                aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGAGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCATTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                       >  2:1241346/1‑150 (MQ=255)
                                                                                                                                 ctttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACa                      >  1:1295087/6‑150 (MQ=255)
                                                                                                                                     caCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAGCCGTTTCACCTTTAATTTGTACAGTGc                  >  2:431046/2‑150 (MQ=255)
                                                                                                                                     caCGGCCCATTCCTCATGGAAGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTACATGCGGACTTCGGCAATAGTGACGTCAAAAACTTCAGACACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGc                  >  2:911129/2‑150 (MQ=255)
                                                                                                                                     aaCGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGc                  <  1:59801/150‑1 (MQ=255)
                                                                                                                                          cccATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGttt             <  2:869728/150‑1 (MQ=255)
                                                                                                                                          cccATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGttt             >  1:1474137/1‑150 (MQ=255)
                                                                                                                                             atccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTcc          <  2:762616/150‑1 (MQ=255)
                                                                                                                                             atccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTcc          <  1:1574903/150‑1 (MQ=255)
                                                                                                                                                 atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGGCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc      <  2:1405831/150‑1 (MQ=255)
                                                                                                                                                  tGAGCATTGGGCCGTGAATGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCt     <  1:1696711/150‑1 (MQ=255)
                                                                                                                                                   gAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTg    >  1:1176194/1‑150 (MQ=255)
                                                                                                                                                     ggAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCa  >  2:1244943/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCA  >  NC_000913/1346808‑1347106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑