Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,354,227 T→G 5.2% T98P (ACC→CCC)  sapC ← antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,354,2270TG 5.2% 362.6 / 4.7 133T98P (ACC→CCC) sapCantimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (62/62);  new base G (2/6);  total (65/68)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAC  >  NC_000913/1354078‑1354375
                                                                                                                                                     |                                                                                                                                                    
tGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGt                                                                                                                                                      >  1:1635286/1‑150 (MQ=255)
 gCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGtt                                                                                                                                                     >  2:1499564/1‑150 (MQ=255)
   cAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTgg                                                                                                                                                   >  2:92283/1‑150 (MQ=255)
    aaCCGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgc                                                                                                                                                  <  1:1588841/150‑1 (MQ=255)
     aCAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgcg                                                                                                                                                 <  2:1632733/150‑1 (MQ=255)
      cagcagCGAAGGGATCGCCAGTTAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGAGCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgcgc                                                                                                                                                <  2:455186/150‑1 (MQ=255)
        gcagcGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAg                                                                                                                                              >  2:1575912/1‑150 (MQ=255)
           gcGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGGGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCAACCGTGGGTGGCGCatttg                                                                                                                                           <  1:733412/150‑5 (MQ=255)
            cGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTcc                                                                                                                                          <  2:1679106/150‑1 (MQ=255)
                gggATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTc                                                                                                                                      >  1:1115659/1‑150 (MQ=255)
                 ggATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCa                                                                                                                                     >  1:380559/1‑150 (MQ=255)
                 ggATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGGGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCTGCTCa                                                                                                                                     <  1:544384/150‑1 (MQ=255)
                  gctCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGACCAGTCCCAGGCCACAGATCGTCGCGGCCAGCGTCCCCACAAATTCGCCACCCACGGTTGGCGCAGCTCCGCGCAg                                                                                                                                    >  1:142487/3‑150 (MQ=255)
                  gATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCCACACAAATTCGCCACCCACGGTTGGCGCAGCTCCGCGCAg                                                                                                                                    >  2:632038/1‑150 (MQ=255)
                  gATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCCCGGTTGGCGCAGCTCCGCTCAg                                                                                                                                    >  1:545175/1‑150 (MQ=255)
                  gATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAg                                                                                                                                    <  2:1220216/150‑1 (MQ=255)
                  gATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAg                                                                                                                                    <  2:604257/150‑1 (MQ=255)
                  caTCGACAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCACAGGCGAAGGTGACCAGTGCCAGGACACAGATCGACGCGGCAAGCGTCACCACACATGCGCCACCCCCGGTTGGCGCAGCTCCGATCAg                                                                                                                                    >  1:97099/2‑150 (MQ=255)
                   aTCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGt                                                                                                                                   >  1:455949/1‑150 (MQ=255)
                    tCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTa                                                                                                                                  <  2:674870/150‑1 (MQ=255)
                        cAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCTAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACg                                                                                                                              <  2:776661/150‑1 (MQ=255)
                        cAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACg                                                                                                                              <  1:1621795/150‑1 (MQ=255)
                        cAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACg                                                                                                                              >  1:1175344/1‑150 (MQ=255)
                           tAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCt                                                                                                                           <  2:1401102/150‑1 (MQ=255)
                            aaGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCtt                                                                                                                          <  1:871173/150‑1 (MQ=255)
                             aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTa                                                                                                                         <  2:1477836/150‑1 (MQ=255)
                             aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCACCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCATCCCCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTa                                                                                                                         >  1:1425997/1‑150 (MQ=255)
                               gTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTaaa                                                                                                                       <  2:540094/150‑1 (MQ=255)
                                  tCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa                                                                                                                    >  1:1789794/1‑150 (MQ=255)
                                   ccAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCAGAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACAt                                                                                                                   <  2:120801/150‑1 (MQ=255)
                                   ccAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCTCTCAGTAAACGGCTTAAAACAt                                                                                                                   <  2:216352/150‑1 (MQ=255)
                                     aGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATcg                                                                                                                 <  2:797477/150‑1 (MQ=255)
                                       aaTATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTTAGTAAACGGCTTAAAACATcgcg                                                                                                               <  1:1271176/150‑1 (MQ=255)
                                          atGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTcc                                                                                                            <  1:571554/150‑1 (MQ=255)
                                           tGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCg                                                                                                           >  2:1662268/1‑150 (MQ=255)
                                             gTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg                                                                                                         <  2:251957/150‑1 (MQ=255)
                                             gTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg                                                                                                         >  1:1380103/1‑150 (MQ=255)
                                               tAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAGCGGCTTAAACCATCGCGTCCGAGgt                                                                                                       >  1:1705541/1‑150 (MQ=255)
                                               tAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgt                                                                                                       >  1:587852/1‑150 (MQ=255)
                                               tAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgt                                                                                                       <  1:1583463/150‑1 (MQ=255)
                                               tAAGCAACGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACGAGGCCACAGATCGTCGCGGCATGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGTCTTATAACATCGCGTCCGAGgt                                                                                                       <  2:750393/150‑1 (MQ=255)
                                                 aGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcg                                                                                                     >  1:1100713/1‑150 (MQ=255)
                                                  gCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgt                                                                                                    >  2:1073454/1‑150 (MQ=255)
                                                   cACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgtc                                                                                                   <  2:1652364/150‑1 (MQ=255)
                                                   cACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGggcgtc                                                                                                   <  2:237377/150‑1 (MQ=255)
                                                    aCCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCa                                                                                                  >  2:592794/1‑150 (MQ=255)
                                                     ccGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATCCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAg                                                                                                 <  2:1305999/150‑1 (MQ=255)
                                                       gCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTc                                                                                               <  2:1625700/150‑1 (MQ=255)
                                                       gCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTc                                                                                               >  2:434103/1‑150 (MQ=255)
                                                        cTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTcc                                                                                              <  1:256994/150‑1 (MQ=255)
                                                         tGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTccc                                                                                             <  1:1022833/150‑1 (MQ=255)
                                                              ggaggaCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga                                                                                        <  2:1173735/150‑1 (MQ=255)
                                                              ggAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga                                                                                        >  1:1267014/1‑150 (MQ=255)
                                                                    cgggtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACACATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGCGGGCGTCAGTCCCCAGGaagaaag                                                                                  <  2:189719/147‑1 (MQ=255)
                                                                     gtgtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGaagaaaga                                                                                 <  1:1787871/150‑1 (MQ=255)
                                                                      tgtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAagaaagaa                                                                                <  2:539205/150‑1 (MQ=255)
                                                                       gtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCGCTGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAgaaagaaa                                                                               >  2:867095/1‑150 (MQ=255)
                                                                          tCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCCCGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACtt                                                                            >  1:1755146/1‑150 (MQ=255)
                                                                            gCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCCCGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCg                                                                          >  1:94921/1‑150 (MQ=255)
                                                                             ccccGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACAACAAATGCGCCACCCACGGATGGCGCAGCTCCGCTCCGTAAACGGCGTAAAACATCGCGTACGAGGTAGTCAGTCCCCAGGAAGAAAGACGCTTCGc                                                                         >  2:1354862/1‑150 (MQ=255)
                                                                              cccGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCCCCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACAGCGCGTCCGAGGGCGTCAGTCCCCAGGAAGAAAGAAACTTCGcc                                                                        >  2:1359029/1‑150 (MQ=255)
                                                                              cccGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGcc                                                                        <  2:881308/150‑1 (MQ=255)
                                                                                   cGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCAACACAAATGCGCCACCCACGGTTGGCGCCGCTCCGCTCAGTAAACGGCTTAAAACCACGCGACCGAGGTCGTCAGACCCCAGGAAGAAAGAAACTTCGCAAAAgc                                                                   >  1:349966/1‑150 (MQ=255)
                                                                                    caaGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAgcg                                                                  >  1:664762/2‑150 (MQ=255)
                                                                                     aaGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAACACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAgcgc                                                                 >  1:107707/1‑150 (MQ=255)
                                                                                     aaGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTAGTCAGTCCCCAGGATGAAAGAAACTTCGCCATTgcgc                                                                 >  2:1123393/1‑150 (MQ=255)
                                                                                      aGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGcgcg                                                                <  1:524925/150‑1 (MQ=255)
                                                                                       ggTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACCTCGCGTCCGAGGTAGTCAGTCCCCAGCAAGAAACAAACTTCGCCATAgcgacc                                                               >  2:158804/1‑147 (MQ=255)
                                                                                       ggTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGa                                                               <  1:1150375/150‑1 (MQ=255)
                                                                                        gTGCCCCGTACCAGGCCACAGAGCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGTAAGAAAGAAACTTCGCCATAGCGCGAc                                                              <  1:1265199/150‑1 (MQ=255)
                                                                                        gTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCCTGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGcgcgcc                                                              >  1:1711662/1‑148 (MQ=255)
                                                                                                  ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggcg                                                    >  2:885820/1‑150 (MQ=255)
                                                                                                   cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGCGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGActgcgg                                                   >  2:1288679/1‑150 (MQ=255)
                                                                                                   cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggcgg                                                   >  1:47047/1‑150 (MQ=255)
                                                                                                    aGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACggcggc                                                  >  1:507347/1‑150 (MQ=255)
                                                                                                    aGGCCACAGAGCGTCGCGGAAAGCGTCACCACAAATGCGCCACCAACGGTTGGCGAAGCTCCGCTCAGTAAACTGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACggcggc                                                  <  1:597841/150‑1 (MQ=255)
                                                                                                       ccACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAACCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGCCCATGACGGCGGCaat                                               >  2:1238261/1‑150 (MQ=255)
                                                                                                        cacaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaata                                              >  2:705569/1‑150 (MQ=255)
                                                                                                           aGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAAtt                                           <  2:904733/150‑1 (MQ=255)
                                                                                                           aGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAAtt                                           <  2:1115659/150‑1 (MQ=255)
                                                                                                            gATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTg                                          >  1:679973/1‑150 (MQ=255)
                                                                                                              tcgtcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCACTAAACGGCTTAAAACATCGCGTCCGAGGACGTCAGTCCCCAGGAAGAAAGAAACTTCGCAATAGCGCGACCATGACGGCGGCAATAAGTGAt                                        >  1:907395/1‑150 (MQ=255)
                                                                                                                  cgcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAAcc                                    <  1:1575912/150‑1 (MQ=255)
                                                                                                                   gcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCg                                   <  1:1108265/150‑1 (MQ=255)
                                                                                                                          gCGTCACCACAAATGCGCCACCCACGGGTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAAtt                            <  2:1545513/150‑1 (MQ=255)
                                                                                                                              caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                        <  2:576096/150‑1 (MQ=255)
                                                                                                                              caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                        >  2:1527513/1‑150 (MQ=255)
                                                                                                                               accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                       <  2:403220/150‑1 (MQ=255)
                                                                                                                               accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                       <  1:9085/150‑1 (MQ=255)
                                                                                                                                ccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                      >  2:337463/1‑150 (MQ=255)
                                                                                                                                 cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGCCGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc                     >  2:1598962/1‑150 (MQ=255)
                                                                                                                                 cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc                     <  2:1789794/150‑1 (MQ=255)
                                                                                                                                      ctGCGCCAACCGTGGGTGGCGCAGCTCCGCTCAGTAAACCGCTTAAAACAACGCGTCCGAGCTCCTCAGTCCCCAGCAAGAAAGAAACAACGCCATAGCGCGACCAAGACGGCTGCAAGAAATGATAACCGAGAAAAACCTGGTCGATGc                >  1:1225795/2‑150 (MQ=255)
                                                                                                                                       tGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGcc               <  1:705569/150‑1 (MQ=255)
                                                                                                                                       tGCGCCAACCGTGGGTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGcc               >  2:1002120/1‑150 (MQ=255)
                                                                                                                                           ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTaa           >  1:801041/1‑150 (MQ=255)
                                                                                                                                             acccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg         <  2:1425997/150‑1 (MQ=255)
                                                                                                                                                    gTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc  <  2:1635286/150‑1 (MQ=255)
                                                                                                                                                    gTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc  <  1:1073454/150‑1 (MQ=255)
                                                                                                                                                    gTTGGCGCAGCTCCGCTCAGTAAACGGCGTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGTAATTGCTGGTCGCAGCCGTAAGGCGCAAAc  >  1:318336/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAC  >  NC_000913/1354078‑1354375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: