Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,443,803 G→A 6.0% P251P (CCG→CCA ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,443,8030GA 6.0% 371.8 / 8.1 116P251P (CCG→CCAydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (59/50);  new base A (3/4);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 116 total aligned reads displayed.

TGGACTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTG  >  NC_000913/1443656‑1443951
                                                                                                                                                    |                                                                                                                                                    
tGGACTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTg                                                                                                                                                    <  1:1799204/150‑1 (MQ=255)
 ggACTTCCTGTAAGTGGGCATTCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAATTAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTgg                                                                                                                                                   <  1:1341153/150‑1 (MQ=255)
 ggACTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGGGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTgg                                                                                                                                                   <  2:763795/150‑1 (MQ=255)
  gacgtacTGTAATTGGGCATTCTACTGCGACGGGAAACTTGCCGCAGGAACCGTCCCTGTTGGATGACTAGCTGGACTGGAAGGAAAATAGCGGGCAATTGATAGTGCTGGCACGGGATAACGGCTATCCGTTGCTCGATTTGCCGTGGc                                                                                                                                                  <  2:1298956/144‑1 (MQ=255)
    cTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTTGCACGGGATAACGCCGATCCGTTGCTCGATTTGCCGTGGCaa                                                                                                                                                >  2:303011/1‑150 (MQ=255)
    cTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCaa                                                                                                                                                >  1:459116/1‑150 (MQ=255)
     ttCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCaaa                                                                                                                                               >  2:1745008/1‑150 (MQ=255)
      tCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATTGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAAt                                                                                                                                              >  1:1420224/1‑150 (MQ=255)
      tCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAAt                                                                                                                                              <  2:1461686/150‑1 (MQ=255)
      tCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAAt                                                                                                                                              >  2:1272302/1‑150 (MQ=255)
          gtcaGTGGGCATGCTAGCGCGACGTTAAACTTGCCGCTGGTACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCACGGCAAAtcgag                                                                                                                                          <  1:1159330/147‑5 (MQ=255)
          gTAAGGGGGCACGCTACTGCGACGTTAAACTTGCCGCAGGAACCGGCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCACGGCAAAtcgag                                                                                                                                          <  1:864995/150‑5 (MQ=255)
           tAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCACGGCAAAtcgagc                                                                                                                                         <  1:1169129/150‑6 (MQ=255)
            aaGTGGGCATGCTACTGCGACGTTAAACTCGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCg                                                                                                                                        <  1:1810641/150‑1 (MQ=255)
             aGTGGGCATGCTACTGAGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGt                                                                                                                                       <  2:664119/150‑1 (MQ=255)
              gTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTc                                                                                                                                      <  1:587870/150‑1 (MQ=255)
               tGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTca                                                                                                                                     >  1:1541412/1‑150 (MQ=255)
                gggCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTcaa                                                                                                                                    >  1:235314/1‑150 (MQ=255)
                  gCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGTCTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCATATTACTCGTcaaca                                                                                                                                  >  1:1143907/1‑150 (MQ=255)
                   cATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTcaacaa                                                                                                                                 >  1:1222641/1‑150 (MQ=255)
                     tGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATACCGGCGATCCGTTGCTCGATTTGCCGTGGCATATTACTCGTCACCAAtt                                                                                                                               >  1:891629/1‑150 (MQ=255)
                     tGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGGCCACAAtt                                                                                                                               >  1:897593/1‑150 (MQ=255)
                      gCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTg                                                                                                                              <  2:91623/150‑1 (MQ=255)
                       gtACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGa                                                                                                                             <  1:1651769/149‑1 (MQ=255)
                       cTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGa                                                                                                                             >  2:207033/1‑150 (MQ=255)
                            gCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTa                                                                                                                        <  2:848920/150‑1 (MQ=255)
                             cGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTaa                                                                                                                       >  1:945411/1‑150 (MQ=255)
                               aCGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGc                                                                                                                     >  1:162615/1‑150 (MQ=255)
                                    aaaCTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATgg                                                                                                                >  2:20615/1‑150 (MQ=255)
                                    aaaCTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATgg                                                                                                                >  2:562482/1‑150 (MQ=255)
                                    aaaCTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATgg                                                                                                                <  2:593280/150‑1 (MQ=255)
                                     aaCTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGt                                                                                                               <  1:395382/150‑1 (MQ=255)
                                         tGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCt                                                                                                           >  2:1741278/1‑150 (MQ=255)
                                          gCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTg                                                                                                          >  2:140689/1‑150 (MQ=255)
                                          gCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTg                                                                                                          >  2:186337/1‑150 (MQ=255)
                                           ccGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTgg                                                                                                         <  1:1582670/150‑1 (MQ=255)
                                           ccGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTgg                                                                                                         <  1:1199524/150‑1 (MQ=255)
                                           ccGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTgg                                                                                                         >  2:769283/1‑150 (MQ=255)
                                            cGCCGGAACCGTCACTGGTGGATTCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGAAAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGa                                                                                                        <  1:1091111/150‑1 (MQ=255)
                                             gCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAg                                                                                                       >  2:1461080/1‑150 (MQ=255)
                                             gCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAg                                                                                                       <  2:1776031/150‑1 (MQ=255)
                                             gCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAg                                                                                                       >  2:502210/1‑150 (MQ=255)
                                              cAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGc                                                                                                      <  2:234431/150‑1 (MQ=255)
                                                ggAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGGTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTg                                                                                                    <  2:100050/150‑1 (MQ=255)
                                                    ccGTCACTTGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCg                                                                                                <  1:1326763/150‑1 (MQ=255)
                                                      gTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTa                                                                                              <  2:1740682/150‑1 (MQ=255)
                                                      gTCACTGGTGGATGCCGAGCGGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTa                                                                                              <  1:1653483/150‑1 (MQ=255)
                                                          cTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCa                                                                                          <  1:479257/150‑1 (MQ=255)
                                                          cTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACAGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCa                                                                                          >  2:468987/1‑150 (MQ=255)
                                                            ggtggATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACTGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAgg                                                                                        <  1:827892/150‑1 (MQ=255)
                                                            ggtggATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAgg                                                                                        >  2:16260/1‑150 (MQ=255)
                                                                  tGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTcc                                                                                  <  2:162615/150‑1 (MQ=255)
                                                                   gCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCt                                                                                 >  1:1324997/1‑150 (MQ=255)
                                                                   gCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTCGCACCGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGACAACAATTGACCGTAAGCCATCTTAGCTGCAGCTGGCCGTATGCAGCATTTCCt                                                                                 >  1:1589552/1‑150 (MQ=255)
                                                                    ccGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCtt                                                                                >  1:966133/1‑150 (MQ=255)
                                                                    ccGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCtt                                                                                >  1:1272459/1‑150 (MQ=255)
                                                                           ggACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTgg                                                                         >  1:1780320/1‑150 (MQ=255)
                                                                           ggACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTgg                                                                         >  1:1767958/1‑150 (MQ=255)
                                                                           ggACTGGCAGGAAAATAGCGGGCAATTGAGCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTgg                                                                         <  1:1391634/150‑1 (MQ=255)
                                                                                ggCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTCCTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGAc                                                                    >  1:1264091/1‑150 (MQ=255)
                                                                                ggCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGACGTATCCAGGTTTTCCTTAGAGTGGCCGAc                                                                    >  1:1452870/1‑150 (MQ=255)
                                                                                ggCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGACCTGGCCGTATGCAGGTATTCCTTTGATTGGCCGaa                                                                    >  2:749072/1‑149 (MQ=255)
                                                                                  cAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTg                                                                  >  2:1261751/1‑150 (MQ=255)
                                                                                     gAAAATACAGGGCAAGTGATCGTGCTGGCCCGGGAACACGGCGCTCCGGTGCGCGCGTGGCCGTGGCAAATTACTCGTCCACAAGAGACCGTAAGCGAGGGGCGCTGGAGCTGGCCGAATGCAGGTTTTCCTTTGAGTGGCCGACTGGgt                                                               <  2:1102947/150‑1 (MQ=255)
                                                                                      aaaaTAGCGGGCAATGGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGgtg                                                              <  1:1623269/150‑1 (MQ=255)
                                                                                      aaaaTAGCCGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTTGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGgtg                                                              <  1:1230603/150‑1 (MQ=255)
                                                                                         aTAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCa                                                           >  1:58853/1‑150 (MQ=255)
                                                                                           aGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCaaa                                                         <  1:1413927/150‑1 (MQ=255)
                                                                                            gCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAg                                                        >  2:746434/1‑150 (MQ=255)
                                                                                              gggCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTc                                                      <  1:1417142/150‑1 (MQ=255)
                                                                                               ggCAATTGATTGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAGTTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCg                                                     <  2:1420224/150‑1 (MQ=255)
                                                                                                 cAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGAc                                                   >  1:1601832/1‑150 (MQ=255)
                                                                                                  aaTTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAACTTACTCGTCACCAATTTACCGTAAGCGATGGTCGCTGGAGCTGTGCGTATGCAGGTGTTCCTTTGCGTGGCCGACTCGGTGTAAAAGTCGACa                                                  >  2:79687/1‑150 (MQ=255)
                                                                                                   aTTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCACTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACaa                                                 >  2:846938/1‑150 (MQ=255)
                                                                                                       aTCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGCCCGTACGCGCTGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGAGAACTgg                                             >  1:1136176/1‑150 (MQ=255)
                                                                                                         cGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCATATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggca                                           <  1:1155822/150‑1 (MQ=255)
                                                                                                         cGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGGCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggca                                           <  2:1332893/150‑1 (MQ=255)
                                                                                                         cGTGCTGGCACGGGATAACGGCGAGCCGTTGCTCGATGTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggca                                           <  1:216995/150‑1 (MQ=255)
                                                                                                             cTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggcaggca                                       >  1:1228768/1‑150 (MQ=255)
                                                                                                             cTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggcaggca                                       <  1:746434/150‑1 (MQ=255)
                                                                                                               ggCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGcaggcagg                                     >  2:636040/1‑150 (MQ=255)
                                                                                                                    gggATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTg                                <  2:235314/150‑1 (MQ=255)
                                                                                                                     ggATAACGGCGATCCGTTGCTCGATTTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTg                                >  2:1542616/1‑150 (MQ=255)
                                                                                                                      gATCACGGCGATCCGGTGCTCGATTTGCCGTGGCAAATTTCTCGTCAACAATAGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTgag                              <  1:303011/150‑1 (MQ=255)
                                                                                                                        tAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGaa                            >  1:1308757/1‑150 (MQ=255)
                                                                                                                         aaCGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAAc                           >  1:1492721/1‑150 (MQ=255)
                                                                                                                          aCGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACg                          >  2:531720/1‑150 (MQ=255)
                                                                                                                             gCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGctc                       >  1:1106395/1‑150 (MQ=255)
                                                                                                                                ttCCGTTGCTGGAGTTGCCGTGGCAATTTACTCGTAAACAATTGACCGTAAGCGATGGTCGCTGGAGCAGCCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTgg                    <  1:946557/149‑1 (MQ=255)
                                                                                                                                aTCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTgg                    <  1:562482/150‑1 (MQ=255)
                                                                                                                                      cgagtaaTTTGCCACGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCTGCg              >  2:889799/7‑150 (MQ=255)
                                                                                                                                       gagtaaTTTGCCACGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCgg             >  1:1160942/6‑150 (MQ=255)
                                                                                                                                       gCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCTGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCgg             >  1:1304232/1‑150 (MQ=255)
                                                                                                                                        agtaaTTTGCCACGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggcagg                                         >  2:754488/5‑121 (MQ=255)
                                                                                                                                        agtaaTTTGCCACGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTggcagg                                         <  1:754488/117‑1 (MQ=255)
                                                                                                                                         tCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGgac           <  2:1643915/150‑1 (MQ=255)
                                                                                                                                                 gCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAg    >  1:1171688/1‑150 (MQ=255)
                                                                                                                                                 gCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAg    <  1:649944/150‑1 (MQ=255)
                                                                                                                                                   cGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAgtg  <  2:1295312/150‑1 (MQ=255)
                                                                                                                                                   cGTGGCAAATTACTCGTCAACAATTGAACGTAAGCGATGGTCGCTGGAGCTGGCCGGATGCAGGGTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACACCTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAgtg  >  2:1526370/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGACTTCCTGTAAGTGGGCATGCTACTGCGACGTTAAACTTGCCGCAGGAACCGTCACTGGTGGATGCCGAGCTGGACTGGCAGGAAAATAGCGGGCAATTGATCGTGCTGGCACGGGATAACGGCGATCCGTTGCTCGATTTGCCGTGGCAAATTACTCGTCAACAATTGACCGTAAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTG  >  NC_000913/1443656‑1443951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: