Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,807 C→G 7.8% G515R (GGT→CGT)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8070CG 7.8% 300.4 / 14.0 100G515R (GGT→CGT) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (41/51);  new base G (2/6);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 103 total aligned reads displayed.

CAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC  >  NC_000913/1449658‑1449955
                                                                                                                                                     |                                                                                                                                                    
cAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGAGTGTCATCTTTCGCAGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTAACCGCTTCGATGCCCGTAGCAcc                                                                                                                                                      >  1:1188855/1‑150 (MQ=255)
 aGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg                                                                                                                                                     <  2:938984/150‑1 (MQ=255)
 aGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg                                                                                                                                                     >  2:1735220/1‑150 (MQ=255)
   aTCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGc                                                                                                                                                   >  1:1598297/1‑150 (MQ=255)
    tCGAGTCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                                  <  2:734184/150‑1 (MQ=255)
    tCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                                  <  1:1075903/150‑1 (MQ=255)
      gaggagGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTGCACCGCTTCCATGCCCGTAGCCCCGGCATc                                                                                                                                                >  2:227299/1‑150 (MQ=255)
           gtaaaTTATAAATATGTTCGTGTGTAGTCCCCACGATATTGTGATGTTTAAGCGTTCCGTAGCGCGTGTCATCTTTCGACGTCTCATCGCGCATGGTTTACGCGTTAACACGTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGc                                                                                                                                           <  2:165293/148‑1 (MQ=255)
           ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGAAGc                                                                                                                                           <  1:561563/150‑1 (MQ=255)
           ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGAAGc                                                                                                                                           <  1:1324659/150‑1 (MQ=255)
           ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTAAACACCTTTAAACGCTTCGATGCCCGTAGCACCGGCATCGATGc                                                                                                                                           >  2:335955/1‑150 (MQ=255)
           ggAAATAATAAATATGTTTGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTTATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTGCGATGCCGGTGCTACGGGCATCGAAGc                                                                                                                                           <  2:1172531/150‑1 (MQ=255)
              aaTTATAAATATGTTGGTGTTTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCATCGATGCCGGTGCTACGGGCATCGAAGcggt                                                                                                                                        <  1:146007/150‑4 (MQ=255)
              aaTTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGAAGcggt                                                                                                                                        <  2:526944/150‑4 (MQ=255)
                 tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                     >  2:256128/1‑150 (MQ=255)
                   taAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTg                                                                                                                                   >  1:478146/1‑150 (MQ=255)
                   taAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTg                                                                                                                                   >  1:1801283/1‑150 (MQ=255)
                    aaaTATGTTGGTGTTTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGc                                                                                                                                  <  1:907289/150‑1 (MQ=255)
                    aaaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGc                                                                                                                                  <  2:653645/150‑1 (MQ=255)
                     aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                                 <  1:1678781/150‑1 (MQ=255)
                       tatGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGt                                                                                                                               <  1:1646230/150‑1 (MQ=255)
                             ggggtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCAt                                                                                                                         <  1:994096/147‑1 (MQ=255)
                             ggTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGGCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCAt                                                                                                                         <  2:300056/150‑1 (MQ=255)
                              gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                        <  1:767028/150‑1 (MQ=255)
                               tgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                       >  2:1800244/1‑150 (MQ=255)
                                       aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                               >  2:840486/1‑150 (MQ=255)
                                       aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTGTCGCCGTCTCATCGTGCATGGTTTTCGCTTTACCACCTTTCCCCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATccc                                                                                                               >  2:1188382/1‑149 (MQ=255)
                                        cccACGATATTGTGATCGATAAGCGTGCCGAATCGCGTGTCATCTTTCGCCGTCTCATCGTTCATGGTATTCGCTTTAACTCCTTTCACCGCTTCGATGCCAGTCGCACCGGGCGCGATGCCAATAGTGCCGTTTTAATGGAAGATCCAg                                                                                                              >  2:278909/1‑150 (MQ=255)
                                          cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTTTCATCTTTCTCCGTCTCATCGTGCATTGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                            <  1:1447424/150‑1 (MQ=255)
                                          cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                            >  1:487668/1‑150 (MQ=255)
                                           aCGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCa                                                                                                           <  2:247021/150‑1 (MQ=255)
                                              atatTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCaaaa                                                                                                        >  1:172571/1‑150 (MQ=255)
                                               tatTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTTCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCaataa                                                                                                       <  2:277624/150‑1 (MQ=255)
                                                 ttGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATg                                                                                                     >  1:780830/1‑150 (MQ=255)
                                                 ttGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATg                                                                                                     <  2:1021137/150‑1 (MQ=255)
                                                   gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGTTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                                   <  2:1598594/150‑1 (MQ=255)
                                                   gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                                   >  2:936013/1‑150 (MQ=255)
                                                    tgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCGCATAGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAg                                                                                                  <  1:1159321/150‑1 (MQ=255)
                                                    tgATCGATAAGCGTGCCGTAGCGCGGGTCAGCTTTCGCCGGCTCATCGTGCATGGTTTTCGCTTTATCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAACTGTAg                                                                                                  <  1:1185245/150‑1 (MQ=255)
                                                     gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCGTCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                 >  1:678638/1‑150 (MQ=255)
                                                     gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                 >  2:1244814/1‑150 (MQ=255)
                                                     gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                 <  2:1238042/150‑1 (MQ=255)
                                                        cGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCAt                                                                                              >  2:777091/1‑150 (MQ=255)
                                                         gATTAGCGTGCCGTAGCGCGTGTCTTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTAATGGAAGATCCAGTCAAAAATGTAGTCATa                                                                                             <  2:1155313/150‑1 (MQ=255)
                                                         gATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATa                                                                                             <  2:750727/150‑1 (MQ=255)
                                                             aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                         <  1:826327/150‑1 (MQ=255)
                                                              gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                        <  2:1251073/150‑1 (MQ=255)
                                                              gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                        <  2:1276936/150‑1 (MQ=255)
                                                              gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                        <  1:1735220/150‑1 (MQ=255)
                                                               ggTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAcc                                                                                       <  1:1280293/149‑1 (MQ=255)
                                                                gagCCGTATCGCGTGTCATCTTTCGCCGTCTCATCTTGCATGGTTTTCGCTTTAACACCTTTCACCTCTTCTATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTAccc                                                                                      <  1:33516/148‑1 (MQ=255)
                                                                gTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAccc                                                                                      >  2:126347/1‑150 (MQ=255)
                                                                    cGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTg                                                                                  >  1:1732139/1‑150 (MQ=255)
                                                                     gTATCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGt                                                                                 <  1:1147978/150‑1 (MQ=255)
                                                                     gTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGTAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGt                                                                                 <  1:1412845/150‑1 (MQ=255)
                                                                      tAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTa                                                                                >  2:921474/1‑150 (MQ=255)
                                                                       atcgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCATCGATGCCCTTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                               <  1:378565/148‑1 (MQ=255)
                                                                       aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                               <  1:1773873/150‑1 (MQ=255)
                                                                       aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                               >  1:99096/1‑150 (MQ=255)
                                                                            gtgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATc                                                                          >  2:1635646/1‑150 (MQ=255)
                                                                              gtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCa                                                                        <  2:613427/150‑1 (MQ=255)
                                                                              gtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCa                                                                        >  1:1788177/1‑150 (MQ=255)
                                                                                  tCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc                                                                    >  2:153342/1‑150 (MQ=255)
                                                                                  tCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc                                                                    >  2:794001/1‑150 (MQ=255)
                                                                                   cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGca                                                                   <  1:1645957/150‑1 (MQ=255)
                                                                                     ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                                 <  1:1544615/150‑1 (MQ=255)
                                                                                        gCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCCCt                                                              >  1:1114666/1‑150 (MQ=255)
                                                                                         ccGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTa                                                             >  1:1721835/1‑150 (MQ=255)
                                                                                          cGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCACGTAGCACCGGCATCTATGCCAAAAGTCAATTTATCATGGAAGATCCAGAAAAAAATGTAGTCATAGTTACCCACTGTACTGATCCATCGCACCACTaa                                                            >  1:1373082/1‑150 (MQ=255)
                                                                                               cATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCACTAACTccc                                                       >  1:1224691/1‑150 (MQ=255)
                                                                                                  cGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGc                                                    <  2:636690/150‑1 (MQ=255)
                                                                                                    tGCATGGTTTTCGCTTTAACACCTTTCCCCGCTTCGATGCCCGTAGCACCTGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTGATAGTTACCCACTGTACTGATACAGCGCACCACTAACTCCCGGCGt                                                  >  1:925339/1‑150 (MQ=255)
                                                                                                      cATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTc                                                >  2:83393/1‑150 (MQ=255)
                                                                                                         ggTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGt                                             <  1:1019842/150‑1 (MQ=255)
                                                                                                         ggTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGt                                             >  2:52671/1‑150 (MQ=255)
                                                                                                         ggTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGt                                             <  1:256128/150‑1 (MQ=255)
                                                                                                         ggTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGt                                             <  2:1801669/150‑1 (MQ=255)
                                                                                                                gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCACTAACTCCCGGCGTTCGGTACTGACg                                      >  2:1099471/1‑150 (MQ=255)
                                                                                                                        acCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTg                              >  2:1799467/1‑150 (MQ=255)
                                                                                                                         ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATACAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                             >  2:1124625/1‑150 (MQ=255)
                                                                                                                          cTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGc                            <  1:1799467/150‑1 (MQ=255)
                                                                                                                           tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCATTAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCCGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                           >  1:1195712/1‑150 (MQ=255)
                                                                                                                           tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                           <  1:82051/150‑1 (MQ=255)
                                                                                                                             tCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcccc                         <  1:1721469/150‑2 (MQ=255)
                                                                                                                             tCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCa                         <  2:1211166/150‑1 (MQ=255)
                                                                                                                                 cGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTc                     <  1:1419488/150‑1 (MQ=255)
                                                                                                                                 cGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCCAGCCCaacgt                     <  1:936013/150‑5 (MQ=255)
                                                                                                                                  gcatCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAATATGTAGTCAAAGTTACCCAATGTACTTAGGCAGCGCACCACTACCTCCCGGCGTTCGGTACTGACGTTGGGCAGGCCCATTTca                    >  2:697205/4‑149 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   >  1:1191319/3‑150 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt                <  2:487668/150‑1 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt                <  1:1440911/150‑1 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTAATCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTCCCCACTGTACTGATCCAGCGGACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCACATTTCCttct                >  2:79720/1‑147 (MQ=255)
                                                                                                                                       gATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg               <  2:1735388/150‑1 (MQ=255)
                                                                                                                                         tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt             <  2:780830/150‑1 (MQ=255)
                                                                                                                                           cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCCGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta           >  1:903692/1‑150 (MQ=255)
                                                                                                                                           cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta           <  2:1011511/150‑1 (MQ=255)
                                                                                                                                           cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta           >  1:1357624/1‑150 (MQ=255)
                                                                                                                                                 gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACAACTAAATCCCGGCTTTCGGTACTGAAGTTGGGCTGGCCCATTTACTGATGCTTATACtaa     >  1:1737551/1‑148 (MQ=255)
                                                                                                                                                    ccGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTccggc  <  1:512702/150‑1 (MQ=255)
                                                                                                                                                    ccGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGAGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGCTGCTTATACTccggc  >  1:317347/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC  >  NC_000913/1449658‑1449955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: