Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,457,530 G→T 5.2% V12L (GTA→TTA)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,457,5300GT 5.2% 355.3 / 6.6 114V12L (GTA→TTA) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base G (53/55);  new base T (3/3);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 114 total aligned reads displayed.

TGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGCCG  >  NC_000913/1457382‑1457677
                                                                                                                                                    |                                                                                                                                                   
tGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGt                                                                                                                                                    >  1:54679/1‑150 (MQ=255)
 ggAAACAAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGAAGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTa                                                                                                                                                   >  1:262537/1‑150 (MQ=255)
      ccAATTACAGTGTGGAACCGGATGATCTGGCGGCTGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgtt                                                                                                                                              <  1:519231/150‑1 (MQ=255)
      ccAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGCCGCGAAGGGGATGGCATGAGCGAACTGCTCGTCAGCCGTCAGCAACGAGTAttgtt                                                                                                                                              >  2:96492/1‑150 (MQ=255)
       cAATTACAGTCTGGAACCGGATGAACTGGCCGCTGGTTTTGTCTTGAGCTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGATTTttgttg                                                                                                                                             <  1:864159/150‑1 (MQ=255)
        aaTTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGc                                                                                                                                            <  2:782092/150‑1 (MQ=255)
            aCAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAc                                                                                                                                        <  2:89937/150‑1 (MQ=255)
            aCAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAc                                                                                                                                        <  2:78475/150‑1 (MQ=255)
                 cTGGCACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGTCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTa                                                                                                                                   <  2:462571/150‑1 (MQ=255)
                    gAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAcc                                                                                                                                >  1:1598755/1‑150 (MQ=255)
                      aCCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGt                                                                                                                              <  2:647676/150‑1 (MQ=255)
                       ccGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTc                                                                                                                             <  1:1821918/150‑1 (MQ=255)
                       ccGGAGGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTc                                                                                                                             <  1:938956/150‑1 (MQ=255)
                        cGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTcc                                                                                                                            <  1:1250451/150‑1 (MQ=255)
                           aTGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCccgc                                                                                                                         <  2:301156/150‑1 (MQ=255)
                               aCTGGCCGCAGGTTATGTGTGGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCa                                                                                                                     <  2:1167850/150‑1 (MQ=255)
                               aCTGGCCGCAGGTTATGTGTGGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGGGGTTGACTTTGACGAGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCa                                                                                                                     <  2:1204225/150‑1 (MQ=255)
                                cTGGCCGCAGGGTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGAGGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCAc                                                                                                                    <  1:19988/150‑1 (MQ=255)
                                  ggCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGt                                                                                                                  >  2:38846/1‑150 (MQ=255)
                                    ccGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGCCCCTTAACCGTCCCGCCGCACGGaa                                                                                                                >  1:60119/1‑150 (MQ=255)
                                    ccGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTaa                                                                                                                <  2:226395/150‑1 (MQ=255)
                                    cagCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAAAGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTaa                                                                                                                >  1:1373215/3‑150 (MQ=255)
                                        aGGTTAGGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGGGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATgcg                                                                                                            <  1:583870/150‑1 (MQ=255)
                                               gtgtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaata                                                                                                     <  1:908281/150‑1 (MQ=255)
                                                tgtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataa                                                                                                    >  1:507748/1‑150 (MQ=255)
                                                 gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataat                                                                                                   >  2:442908/1‑150 (MQ=255)
                                                 gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataat                                                                                                   >  2:436149/1‑150 (MQ=255)
                                                 gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataat                                                                                                   >  2:208631/1‑150 (MQ=255)
                                                  ttGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATg                                                                                                  <  2:946649/150‑1 (MQ=255)
                                                   tGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGc                                                                                                 <  1:1661120/150‑1 (MQ=255)
                                                    gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCTGCAACGAGGATTGTTGCTGACCCTTAGCCGTCCCGCCGCACGTAATGCGCTAAATAATGcc                                                                                                >  2:111758/1‑150 (MQ=255)
                                                    gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGTAACGAGTATTGTTGCTGCCCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGcc                                                                                                >  2:1156703/1‑150 (MQ=255)
                                                      gTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCct                                                                                              >  2:898174/1‑150 (MQ=255)
                                                         gCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCctgct                                                                                           >  1:243060/1‑150 (MQ=255)
                                                         gCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCctgct                                                                                           >  1:405320/1‑150 (MQ=255)
                                                           cAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGa                                                                                         >  1:1335363/1‑150 (MQ=255)
                                                            atgCACTGACGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCATTAAACGTCCCGCCGCCCGTAATGCGCTAAAGAATGACCTGCTGAt                                                                                        >  1:1239402/3‑150 (MQ=255)
                                                            aGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGAt                                                                                        <  1:533256/150‑1 (MQ=255)
                                                            aGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGAt                                                                                        <  2:54679/150‑1 (MQ=255)
                                                             ggCACTGCCGCTGACCAGTGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCAGCACGTAATGCGCTAAATAATGCCCTGCTGATg                                                                                       >  2:768447/1‑150 (MQ=255)
                                                              gCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGc                                                                                      <  1:38846/150‑1 (MQ=255)
                                                               cACTGCCGCTGACCAGCGATGTGGTGGGTGACTTTGAAGCGAAGGGGTTGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCa                                                                                     <  1:523291/150‑1 (MQ=255)
                                                                aCTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCaa                                                                                    <  1:541166/150‑1 (MQ=255)
                                                                  tGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACt                                                                                  <  1:1539411/150‑1 (MQ=255)
                                                                    ccGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTgg                                                                                >  2:1583630/1‑150 (MQ=255)
                                                                     cGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGt                                                                               <  2:843322/150‑1 (MQ=255)
                                                                     cGCTGAACATCGATGTGGTGGTTGACATTGATGCGAAGGGGATGGCATGAGAGAACTGATCGTCAGCCGTCAGCATCGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGGTAAATAATGCCCTGCTGATGCAACTGGt                                                                               <  1:1147268/150‑1 (MQ=255)
                                                                        tGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTaaa                                                                            <  1:1752325/150‑1 (MQ=255)
                                                                         gACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGTCATGATCGAACTGATCGTCAGCCGTCAGCAATGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACTAAATGCGCTAAATAATGCCCTGCTGATTCAACTGGTAAAt                                                                           <  2:1573348/150‑1 (MQ=255)
                                                                           ccAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGa                                                                         >  1:379775/1‑150 (MQ=255)
                                                                           ccAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGa                                                                         >  1:1346/1‑150 (MQ=255)
                                                                            cAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGaa                                                                        >  1:1403656/1‑150 (MQ=255)
                                                                            cAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGaa                                                                        >  1:852292/1‑150 (MQ=255)
                                                                                  tgtgGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGaa                                                                  <  1:596172/150‑1 (MQ=255)
                                                                                      gtggtgGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTGCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTg                                                              <  1:176835/150‑1 (MQ=255)
                                                                                        ggtTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCg                                                            >  2:499848/1‑150 (MQ=255)
                                                                                         gtTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCgg                                                           <  1:955332/150‑1 (MQ=255)
                                                                                          ttGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTATATGAACTGGAAGCTGCGGc                                                          <  1:1114305/150‑1 (MQ=255)
                                                                                          ttGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGc                                                          <  2:129356/150‑1 (MQ=255)
                                                                                           tGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGGCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCt                                                         <  2:229789/150‑1 (MQ=255)
                                                                                            gACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTa                                                        >  1:1064312/1‑150 (MQ=255)
                                                                                              cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      <  2:1598755/150‑1 (MQ=255)
                                                                                              cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      >  2:448263/1‑150 (MQ=255)
                                                                                              cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      >  1:1725671/1‑150 (MQ=255)
                                                                                               ttagACGCGAAGGGGATGGCATGAGCCAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAACCTTAACCGTCCCGCCGCACGTAATCCGCTAAATAAAGCCCTGCTGATGCAACTCGTAAATGAACTGGAATCTGCGGCTACCg                                                     >  1:182455/4‑150 (MQ=255)
                                                                                                   aCGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATAc                                                 <  2:1775350/150‑1 (MQ=255)
                                                                                                    cgcgAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATAcc                                                <  1:1398870/150‑1 (MQ=255)
                                                                                                      cgAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAg                                              >  1:609308/1‑150 (MQ=255)
                                                                                                       gAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAAATGGAAGCTGCGGCTACCGATACCAGc                                             >  2:1799021/1‑150 (MQ=255)
                                                                                                            ggATGGCATGAGCGAACTGATCGTCAGCCGGCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTc                                        <  2:1271216/150‑1 (MQ=255)
                                                                                                                ggCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTc                                    <  2:956950/150‑1 (MQ=255)
                                                                                                                 gCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGAAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCt                                   >  1:925972/1‑150 (MQ=255)
                                                                                                                  cATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtg                                  >  2:1330317/1‑150 (MQ=255)
                                                                                                                  cATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtg                                  >  1:395450/1‑150 (MQ=255)
                                                                                                                  cATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtg                                  <  1:208631/150‑1 (MQ=255)
                                                                                                                  cATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACAGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtg                                  >  2:1209998/1‑150 (MQ=255)
                                                                                                                   aTGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgt                                 <  1:1689300/150‑1 (MQ=255)
                                                                                                                     gAGCGAACTGATCGTCAGCCGTCATCAACGAGTTTTGATGCTGACCGATAACCGTCCCGCCGCACGTAATGAGCTTAATAATGCCCTGCTGATGCTACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgt                               <  1:327136/150‑1 (MQ=255)
                                                                                                                     gAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgt                               <  1:407140/150‑1 (MQ=255)
                                                                                                                      aGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgtg                              <  1:1268595/150‑1 (MQ=255)
                                                                                                                        cGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGAt                            <  1:1629303/150‑1 (MQ=255)
                                                                                                                         gAACTGATCGTCAGCCGTCAGCAAGGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTAGGTCTGTGTGAtt                           >  1:212721/1‑150 (MQ=255)
                                                                                                                              gATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCgg                      <  1:391180/150‑1 (MQ=255)
                                                                                                                              gATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCgg                      >  1:1412869/1‑150 (MQ=255)
                                                                                                                               aTCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAAAAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGt                     <  2:173216/150‑1 (MQ=255)
                                                                                                                                 cgtcagccgtcagcAACGAGTATTGTTGCTGCCCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGCTACCAGCATTTCGGTCTGTGTGATTACCGGTaa                   >  1:754325/1‑150 (MQ=255)
                                                                                                                                  gtcagccgtcagcAACGAGTATTGTTGCTTACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAAt                  <  2:1648222/150‑1 (MQ=255)
                                                                                                                                  gtcagccgtcagcAACGAGTATTGTTGCTGACCGTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAAt                  <  2:400637/150‑1 (MQ=255)
                                                                                                                                  gtcagccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAAt                  >  1:1190387/1‑150 (MQ=255)
                                                                                                                                   tcagccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATg                 <  1:159602/150‑1 (MQ=255)
                                                                                                                                   tcagacttcagcAACGTGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATg                 <  2:1373215/150‑1 (MQ=255)
                                                                                                                                    cagccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAAAAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGAGGCTACAGATACCAGCATTTCGGTCTGTGTGATTACCGGGAATGc                >  2:910401/1‑150 (MQ=255)
                                                                                                                                        ggtcagcAACAATACTCGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCACTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACAGGTAATGCACGc            >  1:677295/2‑150 (MQ=255)
                                                                                                                                         gtcagcAACAATACTCGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTATTGCACGcg           >  1:706362/1‑149 (MQ=255)
                                                                                                                                         gtcagcAACAATACTCGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCt           >  1:1243694/1‑150 (MQ=255)
                                                                                                                                            agcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATAAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCtttt        >  1:661236/1‑150 (MQ=255)
                                                                                                                                             gcAACGAGTATTGTTGCTGACCCTTAACCGCCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGAAGCAACTGGTAAAACAACTGTAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCttttt       >  1:1483498/1‑150 (MQ=255)
                                                                                                                                               aaCGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCCATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTg     >  2:1226273/1‑150 (MQ=255)
                                                                                                                                               aaCGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATCCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTg     <  2:1138696/150‑1 (MQ=255)
                                                                                                                                                 cGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGcc   <  2:507748/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGCCG  >  NC_000913/1457382‑1457677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: