Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,138 G→A 6.1% pseudogene (747/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,1380GA 6.1% 320.8 / 2.9 114pseudogene (747/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (37/68);  new base A (4/3);  total (41/73)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAT  >  NC_000913/1465989‑1466287
                                                                                                                                                     |                                                                                                                                                     
ggggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACggg                                                                                                                                                       <  1:1223235/150‑1 (MQ=255)
  ggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCGCCGGTACGGACATCAACGGTAAGAACGGGaa                                                                                                                                                     >  2:463532/1‑150 (MQ=255)
    tGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAg                                                                                                                                                   <  1:1093325/150‑1 (MQ=255)
     gAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGt                                                                                                                                                  >  1:427021/1‑150 (MQ=255)
     gAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGt                                                                                                                                                  >  2:574488/1‑150 (MQ=255)
     gAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGt                                                                                                                                                  >  2:567095/1‑150 (MQ=255)
     gAGCGCAGATGGCGCAACGAAGTGGAAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGt                                                                                                                                                  <  2:1603968/150‑1 (MQ=255)
          cAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTc                                                                                                                                             <  1:1780424/150‑1 (MQ=255)
             aTGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAgg                                                                                                                                          <  2:941617/150‑1 (MQ=255)
              tGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGa                                                                                                                                         <  1:681268/150‑1 (MQ=255)
               ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGGAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCTACGGTAATAACGGGAAAGTGATTCAGGAc                                                                                                                                        <  2:1728313/150‑1 (MQ=255)
                gcgcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACg                                                                                                                                       >  1:1778363/1‑150 (MQ=255)
                  gcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGc                                                                                                                                     <  1:1810573/150‑1 (MQ=255)
                   aaaCGAATTGGCAATATAATCACAACTGTGAGCTTGTTATTACGGGTGATAATGCCCCAGTAAACAACAATGGCAAAACCACCGGTGACGGTAAAGATTCCACCTTTAAGGAAATAAACGTTAATATCGGGAAATTGATTCATGACGGCg                                                                                                                                    <  2:1459519/149‑1 (MQ=255)
                         aGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTgg                                                                                                                              <  2:1406145/150‑1 (MQ=255)
                          gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                             <  2:988657/150‑1 (MQ=255)
                          gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCAACGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                             <  2:440431/150‑1 (MQ=255)
                           tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGAt                                                                                                                            <  2:1542385/150‑1 (MQ=255)
                            ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                           <  1:1393763/150‑1 (MQ=255)
                            ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                           <  2:1092466/150‑1 (MQ=255)
                            ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAAAGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                           <  2:1307954/150‑1 (MQ=255)
                              cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTc                                                                                                                         >  2:1064270/1‑150 (MQ=255)
                              cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTc                                                                                                                         >  2:423351/1‑150 (MQ=255)
                              cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg                                                                                                                           >  1:1181163/1‑148 (MQ=255)
                              cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg                                                                                                                           <  2:1181163/148‑1 (MQ=255)
                               aaTATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCa                                                                                                                        <  1:1705805/150‑1 (MQ=255)
                               aaTATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCa                                                                                                                        <  1:1698884/150‑1 (MQ=255)
                                    aaTCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggc                                                                                                                   <  2:1443353/150‑1 (MQ=255)
                                    aaTCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggc                                                                                                                   >  1:603726/1‑150 (MQ=255)
                                      tCACAACGGTTAGCTTGTTATTACGGTTGATAATGCCACAGTAAACAACAATGGCAAAACCACGGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                 <  2:735500/150‑1 (MQ=255)
                                      tCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                 >  1:1239077/1‑150 (MQ=255)
                                      tCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTAGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                 >  1:904449/1‑150 (MQ=255)
                                       cacaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                <  2:760248/150‑1 (MQ=255)
                                        acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                               <  2:145334/150‑1 (MQ=255)
                                        acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                               >  1:1085388/1‑150 (MQ=255)
                                         ccaCGGGGAGCGTGTTATTACGGGTGATAATGCCACAGTAAACAACAATCGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                                                                                                              <  1:1758978/148‑1 (MQ=255)
                                         caACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                                                                                                              >  1:1181095/1‑150 (MQ=255)
                                             ggTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                                                                                                          <  2:1163658/150‑1 (MQ=255)
                                             ggTGAGCTTGTTAATACGGGTGATAATGCCACAGTAAACAACAATGCCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGTACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                                                                                                          <  1:13529/150‑1 (MQ=255)
                                               tGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCATCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                                                                                                        <  1:431539/150‑1 (MQ=255)
                                                 aGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTa                                                                                                      <  2:780772/150‑1 (MQ=255)
                                                  gCTTGTTATTTCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCTTTGACGGTATAGATTCAACCAGAACAGATATCAACGAAAAAAACGGGAAAGTGATTCAGGCCGGCGATCTCGCTGTCAGCGGCGGCGGTCACGGTAt                                                                                                     >  1:281485/1‑150 (MQ=18)
                                                  gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt                                                                                                     <  1:1675858/150‑1 (MQ=255)
                                                  gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                <  1:1522824/139‑1 (MQ=255)
                                                  gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                >  2:1522824/1‑139 (MQ=255)
                                                   cTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt                                                                                                    >  2:533381/1‑150 (MQ=255)
                                                    ttGTTAAAACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTCACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAAAAACAGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg                                                                                                   <  2:1309681/150‑1 (MQ=17)
                                                     tGTTATTACGGGTGAGACTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATGCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                  <  2:248386/150‑1 (MQ=255)
                                                           tccGGGTGATAATGCCAAAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                            <  2:1212500/148‑1 (MQ=255)
                                                             cGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                          >  1:1564701/1‑150 (MQ=255)
                                                                  gATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGAc                                                                                     <  2:1778363/150‑1 (MQ=255)
                                                                   aaaaTGCCACAGTAAACAACAATGGCAAAACCACCGTTTACTGAAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACa                                                                                    <  1:775906/148‑1 (MQ=255)
                                                                   aTAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                               >  2:1708588/1‑123 (MQ=255)
                                                                   aTAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                               <  1:1708588/123‑1 (MQ=255)
                                                                     aaTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc                                                                                  <  2:1022367/150‑1 (MQ=255)
                                                                     aaTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc                                                                                  >  2:1076387/1‑150 (MQ=255)
                                                                      aTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgcg                                                                                 <  1:1578923/150‑1 (MQ=255)
                                                                        gCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGcgcg                                                                               <  2:1256398/150‑1 (MQ=255)
                                                                         ccACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGa                                                                              <  1:11072/150‑1 (MQ=255)
                                                                          cacaGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGAc                                                                             <  1:732627/150‑1 (MQ=255)
                                                                              ctAAACAACAAAGGCAAAACCCCCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTg                                                                         <  1:1588865/149‑1 (MQ=255)
                                                                               tacacaccaCTGGCAAAACCACCGTTGACGGAAAAGATTCCACCGGAACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCATCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                        <  2:1641776/147‑1 (MQ=14)
                                                                               tAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                        <  1:1216700/150‑1 (MQ=255)
                                                                                aaaCAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                       <  2:1181095/150‑1 (MQ=255)
                                                                                aaaCAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                       <  2:1618225/150‑1 (MQ=255)
                                                                                  acaacaATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATa                                                                     >  1:1088044/1‑150 (MQ=255)
                                                                                   caacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTgg                                                                        >  2:1614032/1‑146 (MQ=12)
                                                                                    aacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAAc                                                                   <  2:1767924/150‑1 (MQ=255)
                                                                                    aacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAAc                                                                             >  1:481927/1‑140 (MQ=255)
                                                                                      caaTGGCAAAACCACCGTTGACGGTAAAGTTTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGAGACAGCGCGACGGTGGATAACaa                                                                 >  2:782560/1‑150 (MQ=255)
                                                                                      caaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACaa                                                                 <  2:1548244/150‑1 (MQ=255)
                                                                                       aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACTAg                                                                <  1:613582/150‑1 (MQ=255)
                                                                                       aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAg                                                                <  1:1437520/150‑1 (MQ=255)
                                                                                            cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                                                                                                         <  2:359785/104‑1 (MQ=12)
                                                                                            cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                                                                                                         >  1:359785/1‑104 (MQ=25)
                                                                                            cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAg                                                                                                                                                   >  1:166778/1‑62 (MQ=25)
                                                                                            cAAAACCACCGATGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATACTGGACGGCGATCAGGATGTCAGCGGCGGCGGTCACGGTATTGATATAACAGGTGACAGCGCGACGCTCGATAACAAGGGCAc                                                           >  1:248689/1‑150 (MQ=14)
                                                                                             aaaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACTGCGCGACGGTGGATAACAAGGGCAcc                                                          <  2:1217608/150‑1 (MQ=255)
                                                                                                  caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCTACGGTAATAACGGGAAAGTGATTCAGGACGGCAATCTGTATGT‑‑‑CGGCGGCGTTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGt                                                  >  2:928794/1‑150 (MQ=14)
                                                                                                    ccGTTGACGGTAAAGTTTCCACCGGTACGTAAATCAACTGTAAAAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                   <  2:762142/150‑1 (MQ=255)
                                                                                                    ccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACGGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                   <  1:1026172/150‑1 (MQ=255)
                                                                                                    ccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                   <  1:518106/150‑1 (MQ=255)
                                                                                                            ggTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGAt                                           >  2:457193/1‑150 (MQ=255)
                                                                                                              tAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                            >  1:1119651/1‑67 (MQ=255)
                                                                                                              tAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGCAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                            <  2:1119651/67‑1 (MQ=255)
                                                                                                                 aGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGAGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                      >  1:957/1‑150 (MQ=255)
                                                                                                                    ttCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                   >  1:1424880/1‑150 (MQ=39)
                                                                                                                    ttCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                   <  1:1202228/150‑1 (MQ=39)
                                                                                                                      ccACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                 >  1:1464858/1‑150 (MQ=39)
                                                                                                                      ccACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                 >  1:183292/1‑150 (MQ=39)
                                                                                                                       cACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAt                                >  2:76663/1‑150 (MQ=39)
                                                                                                                           ggTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGt                            <  1:531415/150‑1 (MQ=255)
                                                                                                                               cGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATcc                        >  1:74266/1‑150 (MQ=17)
                                                                                                                               cGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATCTCACCGGCGGCGGTCACGGTATTGAAATCACCGGAGACAGCGAGACGGAGGATAACAAGGGCCCCAAGACCGTCACCGATCCGGAGTCCAGCGGTATcc                        >  1:74269/1‑150 (MQ=11)
                                                                                                                                 gAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAg                      <  1:1570411/150‑1 (MQ=255)
                                                                                                                                      cAACGGTAATAACGGGATAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGa                 <  1:567095/150‑1 (MQ=255)
                                                                                                                                          ggTAATAACGGGAACGTGATGCAGGCCGGCGATCTGGAGGGCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGGGCGACGGTGGATAACAAGGGCAGCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGt             <  1:244095/150‑1 (MQ=14)
                                                                                                                                                 aCGGGAAATTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAgg      <  2:927934/150‑1 (MQ=255)
                                                                                                                                                   gggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGcc    >  2:218452/1‑150 (MQ=255)
                                                                                                                                                     gAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAt  >  2:880630/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAT  >  NC_000913/1465989‑1466287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: