Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,144 G→T 9.0% pseudogene (753/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,1440GT 9.0% 298.5 / 7.1 111pseudogene (753/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (32/68);  new base T (6/5);  total (38/73)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCA  >  NC_000913/1465999‑1466292
                                                                                                                                                 |                                                                                                                                                    
cAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTc                                                                                                                                                  >  2:1202228/1‑150 (MQ=255)
   aTGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAgg                                                                                                                                               <  2:941617/150‑1 (MQ=255)
    tGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGa                                                                                                                                              <  1:681268/150‑1 (MQ=255)
     ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGGAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCTACGGTAATAACGGGAAAGTGATTCAGGAc                                                                                                                                             <  2:1728313/150‑1 (MQ=255)
      gcgcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACg                                                                                                                                            >  1:1778363/1‑150 (MQ=255)
        gcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGc                                                                                                                                          <  1:1810573/150‑1 (MQ=255)
         aaaCGAATTGGCAATATAATCACAACTGTGAGCTTGTTATTACGGGTGATAATGCCCCAGTAAACAACAATGGCAAAACCACCGGTGACGGTAAAGATTCCACCTTTAAGGAAATAAACGTTAATATCGGGAAATTGATTCATGACGGCg                                                                                                                                         <  2:1459519/149‑1 (MQ=255)
            cGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATc                                                                                                                                      <  1:1527946/150‑1 (MQ=255)
               tggggCAATATAATCACAACTGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTgg                                                                                                                                   <  1:396250/147‑1 (MQ=255)
               aGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTgg                                                                                                                                   <  2:1406145/150‑1 (MQ=255)
                gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                                  <  2:988657/150‑1 (MQ=255)
                gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCAACGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGa                                                                                                                                  <  2:440431/150‑1 (MQ=255)
                 tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGAt                                                                                                                                 <  2:1595596/150‑1 (MQ=255)
                 tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGAt                                                                                                                                 <  2:1542385/150‑1 (MQ=255)
                  ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                                <  1:1393763/150‑1 (MQ=255)
                  ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAAAGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                                <  2:1307954/150‑1 (MQ=255)
                   gCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGt                                                                                                                               >  2:1731393/1‑150 (MQ=255)
                    cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTc                                                                                                                              >  2:1064270/1‑150 (MQ=255)
                    cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTc                                                                                                                              >  2:1407734/1‑150 (MQ=255)
                    cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTc                                                                                                                              >  2:423351/1‑150 (MQ=255)
                    cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg                                                                                                                                <  2:1181163/148‑1 (MQ=255)
                    cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg                                                                                                                                >  1:1181163/1‑148 (MQ=255)
                     aaTATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCa                                                                                                                             <  1:1698884/150‑1 (MQ=255)
                     aaTATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCa                                                                                                                             <  1:1705805/150‑1 (MQ=255)
                          aaTCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggc                                                                                                                        <  2:1443353/150‑1 (MQ=255)
                          aaTCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggc                                                                                                                        >  1:603726/1‑150 (MQ=255)
                            tCACAACGGTTAGCTTGTTATTACGGTTGATAATGCCACAGTAAACAACAATGGCAAAACCACGGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                      <  2:735500/150‑1 (MQ=255)
                            tCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTAGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                      >  1:904449/1‑150 (MQ=255)
                             cacaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                     <  2:760248/150‑1 (MQ=255)
                              acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                    <  2:145334/150‑1 (MQ=255)
                              acaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                    >  1:1085388/1‑150 (MQ=255)
                               ccaCGGGGAGCGTGTTATTACGGGTGATAATGCCACAGTAAACAACAATCGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                                                                                                                   <  1:1758978/148‑1 (MQ=255)
                               caACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                                                                                                                   >  1:1181095/1‑150 (MQ=255)
                                   ggTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                                                                                                               <  2:1163658/150‑1 (MQ=255)
                                   ggTGAGCTTGTTAATACGGGTGATAATGCCACAGTAAACAACAATGCCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGTACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                                                                                                               <  1:13529/150‑1 (MQ=255)
                                     tGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCATCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                                                                                                             <  1:431539/150‑1 (MQ=255)
                                       aGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTa                                                                                                           <  2:780772/150‑1 (MQ=255)
                                        gCTTGTTATTTCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCTTTGACGGTATAGATTCAACCAGAACAGATATCAACGAAAAAAACGGGAAAGTGATTCAGGCCGGCGATCTCGCTGTCAGCGGCGGCGGTCACGGTAt                                                                                                          >  1:281485/1‑150 (MQ=18)
                                        gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt                                                                                                          <  1:1675858/150‑1 (MQ=255)
                                        gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                     <  1:1522824/139‑1 (MQ=255)
                                        gCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                     >  2:1522824/1‑139 (MQ=255)
                                         cTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt                                                                                                         >  2:533381/1‑150 (MQ=255)
                                           tGTTATTACGGGTGAGACTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATGCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                       <  2:248386/150‑1 (MQ=255)
                                             ttattaCGGGTGATAATGCCACAGAAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                     <  2:801288/150‑1 (MQ=255)
                                                 tccGGGTGATAATGCCAAAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                                 <  2:1212500/148‑1 (MQ=255)
                                                   cGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                               >  1:1564701/1‑150 (MQ=255)
                                                      gTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGAGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTg                                                                                            <  1:124763/150‑1 (MQ=255)
                                                        gATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGAc                                                                                          <  2:1778363/150‑1 (MQ=255)
                                                         aaaaTGCCACAGTAAACAACAATGGCAAAACCACCGTTTACTGAAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACa                                                                                         <  1:775906/148‑1 (MQ=255)
                                                         aTAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                    <  1:1708588/123‑1 (MQ=255)
                                                         aTAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                    >  2:1708588/1‑123 (MQ=255)
                                                           aaTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc                                                                                       >  2:1076387/1‑150 (MQ=255)
                                                           aaTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc                                                                                       <  2:1022367/150‑1 (MQ=255)
                                                            aTGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgcg                                                                                      <  1:1578923/150‑1 (MQ=255)
                                                              gCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGcgcg                                                                                    <  2:1256398/150‑1 (MQ=255)
                                                               ccACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGa                                                                                   <  1:11072/150‑1 (MQ=255)
                                                                cacaGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGAc                                                                                  <  1:732627/150‑1 (MQ=255)
                                                                 acaGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACg                                                                                 <  2:576745/150‑1 (MQ=255)
                                                                    ctAAACAACAAAGGCAAAACCCCCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTg                                                                              <  1:1588865/149‑1 (MQ=255)
                                                                     tacacaccaCTGGCAAAACCACCGTTGACGGAAAAGATTCCACCGGAACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCATCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                             <  2:1641776/147‑1 (MQ=14)
                                                                     tAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                             <  1:1216700/150‑1 (MQ=255)
                                                                      aaaCAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                            <  2:1618225/150‑1 (MQ=255)
                                                                      aaaCAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGa                                                                            <  2:1181095/150‑1 (MQ=255)
                                                                        acaacaATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATa                                                                          >  1:1088044/1‑150 (MQ=255)
                                                                          aacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAAc                                                                        <  2:1767924/150‑1 (MQ=255)
                                                                           acaaTCGCAAAACCACCGTTGACGGTAAAGATTCGACCGGTACGGAAATCATCTGTAATCACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACa                                                                       <  1:1688146/150‑1 (MQ=14)
                                                                            caaTGGCAAAACCACCGTTGACGGTAAAGTTTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGAGACAGCGCGACGGTGGATAACaa                                                                      >  2:782560/1‑150 (MQ=255)
                                                                            caaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACaa                                                                      <  2:1548244/150‑1 (MQ=255)
                                                                             aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACTAg                                                                     <  1:613582/150‑1 (MQ=255)
                                                                             aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAg                                                                     <  1:1437520/150‑1 (MQ=255)
                                                                                  cAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                                                                                                              >  1:359785/1‑104 (MQ=25)
                                                                                  cAAAACCACCGATGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATACTGGACGGCGATCAGGATGTCAGCGGCGGCGGTCACGGTATTGATATAACAGGTGACAGCGCGACGCTCGATAACAAGGGCAc                                                                >  1:248689/1‑150 (MQ=14)
                                                                                   aaaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACTGCGCGACGGTGGATAACAAGGGCAcc                                                               <  2:1217608/150‑1 (MQ=255)
                                                                                   aaaaCCACCGGTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGCGAAAGCGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCAcc                                                               <  1:1168431/150‑1 (MQ=255)
                                                                                        caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCTACGGTAATAACGGGAAAGTGATTCAGGACGGCAATCTGTATGT‑‑‑CGGCGGCGTTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGt                                                       >  2:928794/1‑150 (MQ=14)
                                                                                          ccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACGGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                        <  1:1026172/150‑1 (MQ=255)
                                                                                          ccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                        <  1:518106/150‑1 (MQ=255)
                                                                                                  ggTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGAt                                                >  2:457193/1‑150 (MQ=255)
                                                                                                    tAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                                 >  1:1119651/1‑67 (MQ=255)
                                                                                                    tAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGCAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                                 <  2:1119651/67‑1 (MQ=255)
                                                                                                       aGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGAGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                           >  1:957/1‑150 (MQ=255)
                                                                                                          ttCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                        >  1:1424880/1‑150 (MQ=39)
                                                                                                          ttCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                        <  1:1202228/150‑1 (MQ=39)
                                                                                                           tCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTcc                                       <  2:427021/150‑1 (MQ=39)
                                                                                                            ccACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                      >  1:1464858/1‑150 (MQ=39)
                                                                                                            ccACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                      >  1:183292/1‑150 (MQ=39)
                                                                                                             cACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAt                                     >  2:76663/1‑150 (MQ=39)
                                                                                                               ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATgg                                   >  1:757301/1‑150 (MQ=40)
                                                                                                                 ggTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGt                                 <  1:531415/150‑1 (MQ=255)
                                                                                                                     cGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATcc                             >  1:74266/1‑150 (MQ=17)
                                                                                                                           tCAACGGTAATAACGGGTAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCg                       <  2:1065809/150‑1 (MQ=255)
                                                                                                                            cAACGGTAATAACGGGATAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGa                      <  1:567095/150‑1 (MQ=255)
                                                                                                                            cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGa                      <  1:574488/150‑1 (MQ=255)
                                                                                                                               cGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACgg                   >  1:1803591/1‑150 (MQ=255)
                                                                                                                                ggTAATAACGGGAACGTGATGCAGGCCGGCGATCTGGAGGGCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGGGCGACGGTGGATAACAAGGGCAGCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGt                  <  1:244095/150‑1 (MQ=14)
                                                                                                                                       aCGGGAAATTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAgg           <  2:927934/150‑1 (MQ=255)
                                                                                                                                         gggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGcc         >  2:218452/1‑150 (MQ=255)
                                                                                                                                           gAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAt       >  2:880630/1‑150 (MQ=255)
                                                                                                                                               gTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACAGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTc   <  2:1085388/150‑1 (MQ=255)
                                                                                                                                                tGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCa  <  2:757301/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCA  >  NC_000913/1465999‑1466292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: