Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,504,884 T→C 5.0% intergenic (+19/+21) insQ → / ← ydcO IS609 transposase B/BenE family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,504,8840TC 5.0% 357.3 / 6.8 120intergenic (+19/+21)insQ/ydcOIS609 transposase B/BenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (57/56);  new base C (2/4);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

Only 100 of 120 total aligned reads displayed.

GAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACC  >  NC_000913/1504736‑1505031
                                                                                                                                                    |                                                                                                                                                   
gAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGTGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTc                                                                                                                                                    <  2:17633/150‑1 (MQ=255)
   cGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCtt                                                                                                                                                 >  1:609452/1‑150 (MQ=255)
    cccGATGTAAATGTCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTc                                                                                                                                                <  1:1553336/149‑1 (MQ=255)
     ccGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGTCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCCCCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCa                                                                                                                                               >  2:1213216/1‑150 (MQ=255)
             aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGTCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTgg                                                                                                                                       >  1:81642/1‑150 (MQ=255)
             aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGAAGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTgg                                                                                                                                       >  2:614725/1‑150 (MQ=255)
               tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggag                                                                                                                                     <  2:1036307/150‑1 (MQ=255)
               tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggag                                                                                                                                     <  2:1707622/150‑1 (MQ=255)
               tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTCTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggat                                                                                                                                     <  1:140036/150‑2 (MQ=255)
                ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCCGGGCGTggagg                                                                                                                                    >  1:1279414/1‑150 (MQ=255)
                ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatc                                                                                                                                    <  2:711077/150‑3 (MQ=255)
                ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatc                                                                                                                                    <  2:1123968/150‑3 (MQ=255)
                   gcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATg                                                                                                                                 <  2:1042052/150‑1 (MQ=255)
                   gcTCGCAAAATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGCTGCAGTCAGGCCGCCCGTTGCAGCAGGCACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGACCTTCAGGGCGTGGAGGATg                                                                                                                                 >  1:1700646/1‑150 (MQ=255)
                    cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGt                                                                                                                                >  2:1667874/1‑150 (MQ=255)
                    cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatccctg                                                                                                                                <  2:1246375/150‑7 (MQ=255)
                      cGCAACATTTTAGCGGCGGGGCCCGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCAGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCa                                                                                                                              <  2:1729518/150‑1 (MQ=255)
                       gCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCaa                                                                                                                             >  1:1523398/1‑150 (MQ=255)
                        cAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAat                                                                                                                            >  1:375645/1‑150 (MQ=255)
                        cAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAat                                                                                                                            >  2:206332/1‑150 (MQ=255)
                        cAACATTTTAGCGGCGGGGCACGCCGTTATTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAat                                                                                                                            >  1:1578036/1‑150 (MQ=255)
                             ttttAGCGGCGGGGCAGGCCGTTCTTGCCTGTGGAGCGATGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGTTCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCgg                                                                                                                       <  2:135189/150‑1 (MQ=255)
                             ttttAGCGGCGGGGCACGCCGTTCTTGCCGTTGGAGAGCTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGACATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGGGGAGGATGTCAATATCgg                                                                                                                       <  2:148302/150‑1 (MQ=255)
                             tattAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGTAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATggg                                                                                                                       <  2:800433/148‑1 (MQ=255)
                              tttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCAACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGt                                                                                                                      >  2:1759380/1‑150 (MQ=255)
                                tAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGttt                                                                                                                    >  1:163474/1‑150 (MQ=255)
                                 aGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTc                                                                                                                   <  2:1194234/150‑1 (MQ=255)
                                   cggcggGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTg                                                                                                                 <  1:1758628/150‑1 (MQ=255)
                                   cggcggGGCACGCCGTTCTTGCCTGGGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCTCCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTg                                                                                                                 <  2:348072/150‑1 (MQ=255)
                                     tcgtggCACTCCTTTCTTGGCTTTGGAGAGATGGTGCAGTCAGGCCGCACGTGTTCGCAGGAACCCACCGAAATGATTCAGGCGACTGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTc                                                                                                               <  2:335462/146‑1 (MQ=255)
                                     gcggGGCACGCCGTGCTTGCCTCTGGCGTGATGGGGCAGTCCGGCCGCCCGGTGCAGCAGGAACCCACCGAAATGAATCAGGCGACAGCATGAACGTAGCAGGGATCCCCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTc                                                                                                               <  2:340521/150‑1 (MQ=255)
                                       ggggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAg                                                                                                             <  2:1521267/150‑1 (MQ=255)
                                        gggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGc                                                                                                            >  1:971910/1‑150 (MQ=255)
                                         ggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCg                                                                                                           >  2:621508/1‑150 (MQ=255)
                                         ggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCg                                                                                                           >  1:504916/1‑150 (MQ=255)
                                           cACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAt                                                                                                         <  1:1387171/150‑1 (MQ=255)
                                             cGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATTGGTTTCTGTCAGCGATTa                                                                                                       <  2:160733/150‑1 (MQ=255)
                                             cGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTa                                                                                                       <  2:791491/150‑1 (MQ=255)
                                             cGCCGTTCTTGCCTGGGGAGAGAGGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTa                                                                                                       <  2:1612633/150‑1 (MQ=255)
                                                cGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAAt                                                                                                    <  2:1206164/150‑1 (MQ=255)
                                                cGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAAt                                                                                                    <  2:1099808/150‑1 (MQ=255)
                                                  ttCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                  <  2:713646/150‑1 (MQ=255)
                                                   tCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCa                                                                                                 <  1:14616/150‑1 (MQ=255)
                                                     ttGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGCACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAac                                                                                               <  1:1111756/150‑1 (MQ=255)
                                                          tgtgGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAAcaccac                                                                                          <  1:176365/150‑1 (MQ=255)
                                                               agagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCATCGATTAAATTCAACACCACTTAAc                                                                                     >  2:393581/1‑150 (MQ=255)
                                                               agagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATACAACACCACGTAAc                                                                                     >  2:386895/1‑150 (MQ=255)
                                                                gagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAGTAAATTCAACACCACGTAACa                                                                                    >  2:1293950/1‑150 (MQ=255)
                                                                     ggTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                               >  2:194679/1‑150 (MQ=255)
                                                                        gCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGCCAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTc                                                                            >  2:1535530/1‑150 (MQ=255)
                                                                           gTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCgg                                                                         <  1:356000/150‑1 (MQ=255)
                                                                            tCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGc                                                                        <  2:1467014/150‑1 (MQ=255)
                                                                              aGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCaa                                                                      <  1:399987/150‑1 (MQ=255)
                                                                               ggCCGCCCGTTGAAGCAGGACCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACGCCACGTAACAAACGCCTCCGGCAAt                                                                     >  2:531880/1‑150 (MQ=255)
                                                                               ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAGCGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                     >  2:1150263/1‑150 (MQ=255)
                                                                               ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                     <  1:1710800/150‑1 (MQ=255)
                                                                                gccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACATCCTGAACGTACCAGGGATCCACGTCCATCAGAGAGTGGAGGAGGTCAATATCGTATTCTTAAAGAGAATAAATTAAAAACCACGTAACAAACGCCTCCCGCAAtt                                                                    >  2:1211609/1‑150 (MQ=255)
                                                                                  cgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTaa                                                                  >  2:1226297/1‑150 (MQ=255)
                                                                                  cgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTaa                                                                  >  2:1421974/1‑150 (MQ=255)
                                                                                     ccGTTGAAGCATGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAATAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                               <  2:881624/150‑1 (MQ=255)
                                                                                     ccGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                               <  2:81642/150‑1 (MQ=255)
                                                                                     ccGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                               <  2:40402/150‑1 (MQ=255)
                                                                                      cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATccc                                                              <  1:1588562/150‑1 (MQ=255)
                                                                                        ttGAAGCAGGAACCCACCGATATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCa                                                            <  1:281432/150‑1 (MQ=255)
                                                                                          gAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCaaa                                                          >  1:203952/1‑150 (MQ=255)
                                                                                            aGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCTATTAATCCCCAAAAc                                                        >  1:162719/1‑150 (MQ=255)
                                                                                              cAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGc                                                      >  1:73742/1‑150 (MQ=255)
                                                                                                ggAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAg                                                    <  2:1543124/150‑1 (MQ=255)
                                                                                                  aaCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGaa                                                  >  1:1580209/1‑150 (MQ=255)
                                                                                                   aCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAc                                                 <  2:293614/150‑1 (MQ=255)
                                                                                                    cccACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACAACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAcc                                                >  2:1243687/1‑150 (MQ=255)
                                                                                                            aaTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCgc                                        >  1:1222809/1‑149 (MQ=255)
                                                                                                             aTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACGCCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAc                                       >  2:1004171/1‑150 (MQ=255)
                                                                                                              tGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCACCACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAcc                                      >  1:888217/1‑150 (MQ=255)
                                                                                                              tGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAcc                                      >  1:1178608/1‑150 (MQ=255)
                                                                                                                aTTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAg                                    >  2:652040/1‑150 (MQ=255)
                                                                                                                aTTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAg                                    >  1:1583170/1‑150 (MQ=255)
                                                                                                                  tCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCg                                  >  2:1531130/1‑150 (MQ=255)
                                                                                                                   cAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGATGTTGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGt                                 <  1:1337311/150‑1 (MQ=255)
                                                                                                                    aGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTc                                <  1:1630729/150‑1 (MQ=255)
                                                                                                                         aCAGCCTGAACGAATCAGGCATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGAAACTCTCAGCGATAAAATTCAACACCACCTAACAAACGCCTCCTGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAAAcc                           <  1:1091020/150‑1 (MQ=255)
                                                                                                                            gCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACATACGCCTCCGGCAATTAATCCCCAAACCGCAGAACCAATCCCGCCCAGCGTCAATCCACt                        >  2:1821289/1‑150 (MQ=255)
                                                                                                                            gCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACt                        <  1:1473144/150‑1 (MQ=255)
                                                                                                                                    gTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTa                <  1:206332/150‑1 (MQ=255)
                                                                                                                                    gTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTACTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGCCCAGCGTCAATCCACTTGCCGTTa                >  1:812924/1‑150 (MQ=255)
                                                                                                                                       gCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCa             <  1:1718938/150‑1 (MQ=255)
                                                                                                                                        cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCACCACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGCCCAGCGTCAATCCACTTGCCGTTACCAg            >  1:1609367/1‑150 (MQ=255)
                                                                                                                                        cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAg            <  2:1725793/150‑1 (MQ=255)
                                                                                                                                        cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAg            >  1:1602353/1‑150 (MQ=255)
                                                                                                                                          gggATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGaa          >  1:985264/1‑150 (MQ=255)
                                                                                                                                            gATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCAAGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAAt        >  1:1528969/1‑150 (MQ=255)
                                                                                                                                              tCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAACAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc      >  1:1423792/1‑150 (MQ=255)
                                                                                                                                              tCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTATGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc      <  2:1461274/150‑1 (MQ=255)
                                                                                                                                              tCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc      >  1:1515351/1‑150 (MQ=255)
                                                                                                                                               ccACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGCACCAATCCCGACCAGCGTCAATCCACGTGCCGTTCCCAGAAATGcc     >  2:1611861/1‑150 (MQ=255)
                                                                                                                                                cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca    <  2:1523398/150‑1 (MQ=255)
                                                                                                                                                cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca    >  2:1447968/1‑150 (MQ=255)
                                                                                                                                                aaCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGTGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca    <  1:54523/149‑1 (MQ=255)
                                                                                                                                                 aCGTCCTTGAGGGCGTGGAGGATGTCAATATCGGTTTGTGTCAGCTATTAAATTCAACAACAAGTAACATACGCCTCCGGCAATTTATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGccac   <  2:852901/150‑1 (MQ=255)
                                                                                                                                                  cGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCATAACCAATCCCTACCAGAGTCAATCCACTTGCCGTTACCAGAAAAGccacc  >  1:1097012/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACC  >  NC_000913/1504736‑1505031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: