Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,504,889 C→A 5.1% intergenic (+24/+16) insQ → / ← ydcO IS609 transposase B/BenE family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,504,8890CA 5.1% 353.8 / 7.5 119intergenic (+24/+16)insQ/ydcOIS609 transposase B/BenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base C (59/53);  new base A (2/4);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

Only 100 of 120 total aligned reads displayed.

GCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGC  >  NC_000913/1504740‑1505036
                                                                                                                                                     |                                                                                                                                                   
cccGATGTAAATGTCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTc                                                                                                                                                     <  1:1553336/149‑1 (MQ=255)
 ccGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGTCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCCCCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCa                                                                                                                                                    >  2:1213216/1‑150 (MQ=255)
 ccGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCa                                                                                                                                                    >  2:1419243/1‑150 (MQ=255)
         aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGTCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTgg                                                                                                                                            >  1:81642/1‑150 (MQ=255)
         aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGAAGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTgg                                                                                                                                            >  2:614725/1‑150 (MQ=255)
           tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggag                                                                                                                                          <  2:1036307/150‑1 (MQ=255)
           tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggag                                                                                                                                          <  2:1707622/150‑1 (MQ=255)
           tGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTCTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggat                                                                                                                                          <  1:140036/150‑2 (MQ=255)
            ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCCGGGCGTggagg                                                                                                                                         >  1:1279414/1‑150 (MQ=255)
            ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatc                                                                                                                                         <  2:711077/150‑3 (MQ=255)
            ggCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatc                                                                                                                                         <  2:1123968/150‑3 (MQ=255)
               gcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATg                                                                                                                                      <  2:1042052/150‑1 (MQ=255)
               gcTCGCAAAATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGCTGCAGTCAGGCCGCCCGTTGCAGCAGGCACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGACCTTCAGGGCGTGGAGGATg                                                                                                                                      >  1:1700646/1‑150 (MQ=255)
                cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatccctg                                                                                                                                     <  2:1246375/150‑7 (MQ=255)
                  cGCAACATTTTAGCGGCGGGGCCCGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCAGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCa                                                                                                                                   <  2:1729518/150‑1 (MQ=255)
                   gCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCaa                                                                                                                                  >  1:1523398/1‑150 (MQ=255)
                    cAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAat                                                                                                                                 >  2:206332/1‑150 (MQ=255)
                    cAACATTTTAGCGGCGGGGCACGCCGTTATTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAat                                                                                                                                 >  1:1578036/1‑150 (MQ=255)
                         ttttAGCGGCGGGGCAGGCCGTTCTTGCCTGTGGAGCGATGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGTTCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCgg                                                                                                                            <  2:135189/150‑1 (MQ=255)
                         ttttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGATATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCgg                                                                                                                            <  2:1218850/150‑1 (MQ=255)
                         ttttAGCGGCGGGGCACGCCGTTCTTGCCGTTGGAGAGCTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGACATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGGGGAGGATGTCAATATCgg                                                                                                                            <  2:148302/150‑1 (MQ=255)
                         tattAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGTAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATggg                                                                                                                            <  2:800433/148‑1 (MQ=255)
                          tttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCAACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGt                                                                                                                           >  2:1759380/1‑150 (MQ=255)
                           ttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGtt                                                                                                                          <  1:390522/150‑1 (MQ=255)
                            tAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGttt                                                                                                                         >  1:163474/1‑150 (MQ=255)
                             aGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTc                                                                                                                        <  2:1194234/150‑1 (MQ=255)
                               cggcggGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTg                                                                                                                      <  1:1758628/150‑1 (MQ=255)
                               cggcggGGCACGCCGTTCTTGCCTGGGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCTCCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTg                                                                                                                      <  2:348072/150‑1 (MQ=255)
                                 tcgtggCACTCCTTTCTTGGCTTTGGAGAGATGGTGCAGTCAGGCCGCACGTGTTCGCAGGAACCCACCGAAATGATTCAGGCGACTGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTc                                                                                                                    <  2:335462/146‑1 (MQ=255)
                                   ggggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAg                                                                                                                  <  2:1521267/150‑1 (MQ=255)
                                    gggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGc                                                                                                                 >  1:971910/1‑150 (MQ=255)
                                     ggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCg                                                                                                                >  2:621508/1‑150 (MQ=255)
                                     ggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCg                                                                                                                >  1:504916/1‑150 (MQ=255)
                                       cACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAt                                                                                                              <  1:1387171/150‑1 (MQ=255)
                                         cGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATTGGTTTCTGTCAGCGATTa                                                                                                            <  2:160733/150‑1 (MQ=255)
                                         cGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTa                                                                                                            <  2:791491/150‑1 (MQ=255)
                                         cGCCGTTCTTGCCTGGGGAGAGAGGGGGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTa                                                                                                            <  2:1612633/150‑1 (MQ=255)
                                            cGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAAt                                                                                                         <  2:1099808/150‑1 (MQ=255)
                                            cGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAAt                                                                                                         <  2:1206164/150‑1 (MQ=255)
                                              ttCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                       <  2:713646/150‑1 (MQ=255)
                                               tCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCa                                                                                                      <  1:14616/150‑1 (MQ=255)
                                                 ttGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGCACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAac                                                                                                    <  1:1111756/150‑1 (MQ=255)
                                                      tgtgGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAAcaccac                                                                                               <  1:176365/150‑1 (MQ=255)
                                                           agagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCATCGATTAAATTCAACACCACTTAAc                                                                                          >  2:393581/1‑150 (MQ=255)
                                                           agagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATACAACACCACGTAAc                                                                                          >  2:386895/1‑150 (MQ=255)
                                                             agaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACaa                                                                                        <  2:1564888/150‑1 (MQ=255)
                                                                 ggTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                                    >  2:1131555/1‑150 (MQ=255)
                                                                 ggTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                                    >  2:194679/1‑150 (MQ=255)
                                                                   tGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCt                                                                                  >  2:1718938/1‑150 (MQ=255)
                                                                       gTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCgg                                                                              <  1:356000/150‑1 (MQ=255)
                                                                          aGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCaa                                                                           <  1:399987/150‑1 (MQ=255)
                                                                           ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAGCGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                          >  2:1150263/1‑150 (MQ=255)
                                                                           ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                          <  2:1518897/150‑1 (MQ=255)
                                                                           ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                          <  1:1710800/150‑1 (MQ=255)
                                                                              cgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTaa                                                                       >  2:1226297/1‑150 (MQ=255)
                                                                              cgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTaa                                                                       >  2:1421974/1‑150 (MQ=255)
                                                                                 ccGTTGAAGCATGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAATAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                                    <  2:881624/150‑1 (MQ=255)
                                                                                 ccGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                                    <  2:81642/150‑1 (MQ=255)
                                                                                 ccGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                                    <  2:40402/150‑1 (MQ=255)
                                                                                  cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATccc                                                                   <  1:1588562/150‑1 (MQ=255)
                                                                                  cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATccc                                                                   <  2:185255/150‑1 (MQ=255)
                                                                                    ttGAAGCAGGAACCCACCGATATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCa                                                                 <  1:281432/150‑1 (MQ=255)
                                                                                      gAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCaaa                                                               >  1:203952/1‑150 (MQ=255)
                                                                                        aGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCTATTAATCCCCAAAAc                                                             >  1:162719/1‑150 (MQ=255)
                                                                                          cAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGc                                                           >  1:73742/1‑150 (MQ=255)
                                                                                            ggAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAg                                                         <  2:1543124/150‑1 (MQ=255)
                                                                                              aaCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGaa                                                       >  1:1580209/1‑150 (MQ=255)
                                                                                               aCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAc                                                      <  2:293614/150‑1 (MQ=255)
                                                                                                cccACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACAACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAcc                                                     >  2:1243687/1‑150 (MQ=255)
                                                                                                        aaTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCgc                                             >  1:1222809/1‑149 (MQ=255)
                                                                                                         aTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACGCCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAc                                            >  2:1004171/1‑150 (MQ=255)
                                                                                                          tGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCACCACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAcc                                           >  1:888217/1‑150 (MQ=255)
                                                                                                          tGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAcc                                           >  1:1178608/1‑150 (MQ=255)
                                                                                                            aTTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAg                                         >  2:652040/1‑150 (MQ=255)
                                                                                                            aTTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAg                                         >  1:1583170/1‑150 (MQ=255)
                                                                                                              tCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCg                                       >  2:1531130/1‑150 (MQ=255)
                                                                                                               cAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGATGTTGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGt                                      <  1:1337311/150‑1 (MQ=255)
                                                                                                                aGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTc                                     <  1:1630729/150‑1 (MQ=255)
                                                                                                                     aCAGCCTGAACGAATCAGGCATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGAAACTCTCAGCGATAAAATTCAACACCACCTAACAAACGCCTCCTGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAAAcc                                <  1:1091020/150‑1 (MQ=255)
                                                                                                                        gCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACt                             <  1:1473144/150‑1 (MQ=255)
                                                                                                                                gTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTa                     <  1:206332/150‑1 (MQ=255)
                                                                                                                                gTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTACTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGCCCAGCGTCAATCCACTTGCCGTTa                     >  1:812924/1‑150 (MQ=255)
                                                                                                                                   gCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCa                  <  1:1718938/150‑1 (MQ=255)
                                                                                                                                    cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCACCACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGCCCAGCGTCAATCCACTTGCCGTTACCAg                 >  1:1609367/1‑150 (MQ=255)
                                                                                                                                    cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAg                 >  1:1602353/1‑150 (MQ=255)
                                                                                                                                    cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAg                 <  2:1725793/150‑1 (MQ=255)
                                                                                                                                     aGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGa                <  2:341702/150‑1 (MQ=255)
                                                                                                                                        gATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGACCCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAAt             >  1:992848/1‑150 (MQ=255)
                                                                                                                                        gATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCAAGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAAt             >  1:1528969/1‑150 (MQ=255)
                                                                                                                                          tCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAAACAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc           >  1:1423792/1‑150 (MQ=255)
                                                                                                                                          tCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTATGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc           <  2:1461274/150‑1 (MQ=255)
                                                                                                                                          tCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc           >  1:1515351/1‑150 (MQ=255)
                                                                                                                                           ccACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGCACCAATCCCGACCAGCGTCAATCCACGTGCCGTTCCCAGAAATGcc          >  2:1611861/1‑150 (MQ=255)
                                                                                                                                            cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca         <  2:1523398/150‑1 (MQ=255)
                                                                                                                                            cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca         >  2:1447968/1‑150 (MQ=255)
                                                                                                                                            aaCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGTGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca         <  1:54523/149‑1 (MQ=255)
                                                                                                                                             aCGTCCTTGAGGGCGTGGAGGATGTCAATATCGGTTTGTGTCAGCTATTAAATTCAACAACAAGTAACATACGCCTCCGGCAATTTATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGccac        <  2:852901/150‑1 (MQ=255)
                                                                                                                                              cGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCATAACCAATCCCTACCAGAGTCAATCCACTTGCCGTTACCAGAAAAGccacc       >  1:1097012/1‑150 (MQ=255)
                                                                                                                                                 ccTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCaccacc    >  1:346458/1‑150 (MQ=255)
                                                                                                                                                   ttCAGGGCGTGCAGGATGTCAATATCGGTTTCTGTCATCGATTAAATTCAAACCCACGTACCAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAAGACCGACCCCCGACAATCCACTTGCCGTTCCCAGAAATGCCACCAccgc  >  1:863134/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGC  >  NC_000913/1504740‑1505036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: