Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,530,496 G→T 5.9% intergenic (+92/‑90) ydcD → / → yncI putative immunity protein for RhsE/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,4960GT 5.9% 294.4 / 3.2 101intergenic (+92/‑90)ydcD/yncIputative immunity protein for RhsE/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (47/46);  new base T (3/5);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGG  >  NC_000913/1530347‑1530644
                                                                                                                                                     |                                                                                                                                                    
ttattaTGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGCACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGCCTGGGCAGGGCAAGATCATGAAAATTGTGATg                                                                                                                                                      >  2:288208/1‑150 (MQ=255)
   ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGCCGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                                                                                                                   >  2:1680479/1‑150 (MQ=255)
     atGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAAt                                                                                                                                                 >  2:1132004/1‑150 (MQ=255)
      tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAAta                                                                                                                                                >  1:606517/1‑149 (MQ=255)
       gATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCa                                                                                                                                               >  1:1787914/1‑150 (MQ=255)
        aTAACAATTTTAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGATATAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCAc                                                                                                                                              <  2:126806/150‑1 (MQ=255)
          aaCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGa                                                                                                                                            >  2:100138/1‑150 (MQ=255)
           aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAt                                                                                                                                           >  1:1719550/1‑150 (MQ=255)
             aaTATGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCATGGCAAGATCATGAAAATTGTGATGTAAATCACGAttt                                                                                                                                         >  2:880932/1‑150 (MQ=255)
                ttGAAAATTTAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACCCCAGAAAGAGAAAAATATAAAATACATACAGAAAGCCATCAGAGCACGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCa                                                                                                                                      <  1:1371322/150‑1 (MQ=255)
                ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGACAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCa                                                                                                                                      <  2:616816/150‑1 (MQ=255)
                 tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGAAAATCACGATTTTCAt                                                                                                                                     >  1:1228086/1‑150 (MQ=255)
                  taaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCTGGGCAAGATCTTGAAAATTGTGATGTAAATCACGATTTTCATc                                                                                                                                    <  1:1507234/149‑1 (MQ=255)
                  gAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtg                                                                                                                                    <  1:1061233/150‑2 (MQ=255)
                     aaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCttt                                                                                                                                 >  2:608274/1‑150 (MQ=255)
                     aaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCttt                                                                                                                                 <  1:1740702/150‑1 (MQ=255)
                      aaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGCACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTg                                                                                                                                >  2:778841/1‑150 (MQ=255)
                       aTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAACTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                               <  2:1566057/150‑1 (MQ=255)
                         gAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAAGCACGATTTTCATCTTTGCtt                                                                                                                             <  2:301237/150‑1 (MQ=255)
                          aattttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATCAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                            <  2:281869/147‑1 (MQ=255)
                           aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                           <  2:236833/150‑1 (MQ=255)
                              ttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACg                                                                                                                        >  2:1018762/1‑150 (MQ=255)
                              ttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACg                                                                                                                        <  1:1526633/150‑1 (MQ=255)
                                   aCTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTAc                                                                                                                   >  2:1150936/1‑150 (MQ=255)
                                    cTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACa                                                                                                                  >  1:1382217/1‑150 (MQ=255)
                                    cTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACa                                                                                                                  >  1:1761244/1‑150 (MQ=255)
                                      ggATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAgg                                                                                                                <  2:565383/150‑1 (MQ=255)
                                         ttGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGa                                                                                                             <  2:584247/150‑1 (MQ=255)
                                          tGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGAt                                                                                                            <  2:1058546/150‑1 (MQ=255)
                                          tGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGAt                                                                                                            <  2:606517/150‑1 (MQ=255)
                                           ggTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATc                                                                                                           >  1:836526/1‑150 (MQ=255)
                                            tttaaTTGTCATATTGAACAACACCATAAAGAGAAAAATATAAAATAAATACTTAAAGACATTATATCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCa                                                                                                          <  1:608077/147‑1 (MQ=255)
                                                  ggTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAttt                                                                                                    >  1:568115/1‑150 (MQ=255)
                                                  ggTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAttt                                                                                                    >  2:844471/1‑150 (MQ=255)
                                                   gtaattaTGAACAACACCTGAAAGATAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGTGCAGTGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTa                                                                                                   <  1:288208/144‑1 (MQ=255)
                                                   gTCATAATTAACATCAGCAGATAGAGATAAATATATAATAAATACATAAAGACTTTAGTGCAAGGGACGGGTCAGGGCAATATCATGAAAATTGTGATGTAAATCAGGATTTTCATCTTTGCTTTAACGCCTACAGGTTATCAAAATTTa                                                                                                   <  1:390873/150‑1 (MQ=255)
                                                   gTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCATGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTa                                                                                                   >  2:303547/1‑150 (MQ=255)
                                                     cATAATGAACAACACCAGAATGAGAAAAATATAATATACATAAAGAAAGACATGAGAGGAAGGGACGGGGCAGGGCAAGATCATGAAATTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                                 <  2:1193131/150‑1 (MQ=255)
                                                      aTAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACATAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                <  1:846800/150‑1 (MQ=255)
                                                      aTAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                <  2:1233509/150‑1 (MQ=255)
                                                      aTAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                <  2:654294/150‑1 (MQ=255)
                                                       tAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACTGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATATTAATCTTTGCTTTAACCCCTACATGATATCAAAATTTAAcca                                                                                               >  1:271296/1‑149 (MQ=255)
                                                       tAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCt                                                                                               >  1:1540852/1‑150 (MQ=255)
                                                           taacaacACCATAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGAGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTgg                                                                                           <  1:301468/149‑1 (MQ=255)
                                                            aacaacACCAGAAAGAGAAAAATAAAAAATACATACCGACAGACATGAGAGGAAGGGACTGGGCAGGGCAAGATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGa                                                                                          <  2:728426/150‑1 (MQ=255)
                                                                 cacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                     >  1:13609/1‑150 (MQ=255)
                                                                    cAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaa                                                                                  <  1:319147/150‑1 (MQ=255)
                                                                     aGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaa                                                                                 >  2:292593/1‑150 (MQ=255)
                                                                      taaaGAGAAAAATATAAAATACATACAGAAAGACATGAGATCAAGGGATGGGGCAGGGCAAGTTCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAATATTTAACCTATGGAATAACTaaaa                                                                                <  1:1499132/149‑1 (MQ=255)
                                                                      gAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaaa                                                                                >  1:538438/1‑150 (MQ=255)
                                                                         agagAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTaa                                                                                                  <  1:649402/129‑1 (MQ=255)
                                                                         agagAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTaa                                                                                                  >  2:649402/1‑129 (MQ=255)
                                                                         agagAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGa                                                                             >  2:283624/1‑150 (MQ=255)
                                                                         agagAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGa                                                                             <  2:445307/150‑1 (MQ=255)
                                                                          gagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                            >  2:1492053/1‑150 (MQ=255)
                                                                          gagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                            <  1:608274/150‑1 (MQ=255)
                                                                            gaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaa                                                                          >  1:1448879/1‑150 (MQ=255)
                                                                                 atatAAAATACATAAATAAAGACATGAGAGCAAGTGACGGGGCAGTGCAAGATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                     <  1:350424/150‑1 (MQ=255)
                                                                                    taAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGAc                                                                  <  2:465467/150‑1 (MQ=255)
                                                                                          acatacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAgg                                                            <  1:692771/150‑1 (MQ=255)
                                                                                          acatacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAgg                                                            <  1:686395/150‑1 (MQ=255)
                                                                                           catacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGt                                                           >  1:848239/1‑150 (MQ=255)
                                                                                            atacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTg                                                          <  1:150622/150‑1 (MQ=255)
                                                                                            ataaaGAAATACATGAGATCAAGGGACGTGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTg                                                          <  1:1119316/150‑1 (MQ=255)
                                                                                             tacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGa                                                         >  1:1805134/1‑150 (MQ=255)
                                                                                              acaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAg                                                        >  2:56337/1‑150 (MQ=255)
                                                                                                     gACATGAGAGCATGGGACGGGGCAGTGCAATATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTATCATTTGTAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCaa                                                 <  2:1612196/150‑1 (MQ=255)
                                                                                                       cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAg                                               <  1:283624/150‑1 (MQ=255)
                                                                                                           agagCAAGGTACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTg                                           <  1:1643308/150‑1 (MQ=255)
                                                                                                            gagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                          >  2:1264163/1‑150 (MQ=255)
                                                                                                            gagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                          <  1:100138/150‑1 (MQ=255)
                                                                                                             agCAAGGGACGGGGCAGGGAAATATAATTAAAATTGTTATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTTATAAAAATTTAACCTTTGTAATAACTAAAAATATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                         <  1:1506574/150‑1 (MQ=255)
                                                                                                               cAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                                       <  1:821458/150‑1 (MQ=255)
                                                                                                               cAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                                       >  2:1590998/1‑150 (MQ=255)
                                                                                                                 aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                     >  2:1343482/1‑150 (MQ=255)
                                                                                                                 aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                     >  1:1115229/1‑150 (MQ=255)
                                                                                                                 aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                     >  1:562586/1‑150 (MQ=255)
                                                                                                                   ggACGGGGCAGGGCAAGATAATTAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTTATCAAAATTTAACCTTTGGAATAAATAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATta                                   <  2:1723462/150‑1 (MQ=255)
                                                                                                                    gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                  >  2:1769498/1‑150 (MQ=255)
                                                                                                                      cGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                <  1:1011345/150‑1 (MQ=255)
                                                                                                                       ggggCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATAtt                               >  1:790764/1‑150 (MQ=255)
                                                                                                                       ggggCAGGGCAAGATCATGAAAATTGTGATGAAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGGGATCAAAATTAACCCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATAtt                               <  2:140260/150‑1 (MQ=255)
                                                                                                                            agggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATACCTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATATCAGt                          <  2:1198358/150‑1 (MQ=255)
                                                                                                                             gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                         >  1:556173/1‑150 (MQ=255)
                                                                                                                                caAGATCTTGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCTTTAACGCCTACATGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                      <  2:271296/150‑1 (MQ=255)
                                                                                                                                agcaaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATGAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATACAAAGTTTGCTTGATTATATTTCAGTTAcc                      >  1:627257/8‑150 (MQ=38)
                                                                                                                                agcaaagaTGAAAATCGTGATTTACAGCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGAATGATTATATTTCAGTGAcc                      >  1:633688/8‑150 (MQ=38)
                                                                                                                                 aaGATCATGAAAATTGTGATGTAAATCATGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc                     <  1:909883/150‑1 (MQ=255)
                                                                                                                                     tCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACACCTGa                 >  1:628318/1‑150 (MQ=255)
                                                                                                                                     agaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGa                 >  1:686143/3‑150 (MQ=255)
                                                                                                                                      cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACATTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTTACCCATGat                >  2:1018159/1‑150 (MQ=255)
                                                                                                                                      cATGAAAATTGTGATGTAAATCACGATTTTCATCATTGCTTTAACGCCTACAGGTGATCAAAATTTACCCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCGTGat                >  1:500913/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAATTGTGATGTAAATCACGATTTTAATCTTTGATTTAACGCCTACAGGTTATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGat                <  1:1533289/149‑1 (MQ=255)
                                                                                                                                           aaaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACg           >  2:1249990/1‑150 (MQ=255)
                                                                                                                                           aaaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACg           <  1:1291841/150‑1 (MQ=255)
                                                                                                                                            aaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGa          >  1:855175/1‑150 (MQ=255)
                                                                                                                                             gcaaagATGAAAATCGTGATTTACATCCTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGac         >  1:1527857/6‑150 (MQ=255)
                                                                                                                                             aTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGac         <  1:1132004/150‑1 (MQ=255)
                                                                                                                                                gtgATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaac      >  1:1530532/1‑150 (MQ=255)
                                                                                                                                                    tGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAgg  >  1:11315/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGG  >  NC_000913/1530347‑1530644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: