Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,590,601 G→C 8.5% intergenic (‑65/+253) yneL ← / ← hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,590,6010GC 8.5% 325.9 / 19.5 108intergenic (‑65/+253)yneL/hipApseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
Reads supporting (aligned to +/- strand):  ref base G (49/49);  new base C (5/4);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

Only 100 of 111 total aligned reads displayed.

CTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590452‑1590747
                                                                                                                                                     |                                                                                                                                                  
ctgTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATg                                                                                                                                                    <  2:1256627/150‑1 (MQ=255)
ctgTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATg                                                                                                                                                    >  1:1523591/1‑150 (MQ=255)
ctgTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGACATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAATGTCTATg                                                                                                                                                    >  1:1233252/1‑150 (MQ=255)
 tgtgGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGc                                                                                                                                                   >  1:970088/1‑150 (MQ=255)
  gtggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATACg                                                                                                                                                  <  2:1632557/150‑1 (MQ=255)
    ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                >  2:172229/1‑150 (MQ=255)
       gACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgacaga                                                                                                                                             >  2:1672485/1‑150 (MQ=255)
             gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGATGTAGCCATTTATGATACCGGAGTGGACTCATTCCCTCTTGATCCGTCCTTAAATAAGTAACAGCACGCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgc                                                                                                                                       <  2:294173/150‑1 (MQ=255)
               aaTGTTCATTCAGCATCTGTCGCCTGCCGTCATAGAGACGTAGCCATTTCTGAGACCGGAGGGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTATCGTATGCAGTGGCTGTCGCATAGACACTgcat                                                                                                                                     <  1:310512/150‑3 (MQ=255)
                 tGTTCATTCAGCATCTGTCGCCTGACTTCTTTGAGACGTAGCCATTTCTGATAGAGGAGTTGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcatac                                                                                                                                   <  1:1090145/150‑5 (MQ=255)
                 tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                   <  1:1092625/150‑1 (MQ=255)
                  gTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctct                                                                                                                                  >  1:337015/1‑150 (MQ=255)
                    tcattcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTa                                                                                                                                <  1:1141714/150‑1 (MQ=255)
                     cattcaGCATCTGTCGCCTGACTTCATTGAGTCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACTGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:623758/150‑1 (MQ=255)
                        tcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                                                            >  1:1103539/1‑150 (MQ=255)
                          aGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGAGCTCTTAGCATgg                                                                                                                          >  1:363134/1‑150 (MQ=255)
                            cATCTGTCGCCTGACTTCATTGCGACGTAGCCTTTTCTGATCCCGGAATGTCCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTAAGCAGTGTCTATGCGACAGCCACTGGGCTCGTAGCATgggg                                                                                                                        >  2:1222840/1‑150 (MQ=255)
                               cTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAg                                                                                                                     >  2:1626094/1‑150 (MQ=255)
                                tGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAgg                                                                                                                    <  1:425902/150‑1 (MQ=255)
                                 gTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGTCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAggg                                                                                                                   <  1:300380/150‑1 (MQ=255)
                                        gACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAAcc                                                                                                            >  1:451285/1‑150 (MQ=255)
                                          cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTATCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                          <  2:199036/150‑1 (MQ=255)
                                          cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                          <  1:89893/150‑1 (MQ=255)
                                            tCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGc                                                                                                        <  1:720111/150‑1 (MQ=255)
                                            tCATTGAGACGTAGACATTTCTGATACCGGAGTGGACTCATTCACGCTTGATCCGTACTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTTTGCAGTGTCTATGCGACAGACCCTGCGCTCTTAGCATGGGGCAGGGAATAACCACGc                                                                                                        >  2:79790/1‑150 (MQ=255)
                                                     cGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTcc                                                                                               <  2:337015/150‑1 (MQ=255)
                                                        aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:1748558/150‑1 (MQ=255)
                                                        aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGAAACTGAGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            >  2:136059/1‑150 (MQ=255)
                                                        aGCAATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:940077/150‑1 (MQ=255)
                                                         gCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGACTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTACCCAGGCTATTTCTCCAttc                                                                                           >  2:1092749/1‑148 (MQ=255)
                                                               tCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGTCAGACACTGCGCTCTTAGGATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGc                                                                                     >  2:345742/1‑150 (MQ=255)
                                                                cTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGcc                                                                                    <  1:1013376/150‑1 (MQ=255)
                                                                      ccGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTAt                                                                              >  1:847811/1‑150 (MQ=255)
                                                                         gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  2:1523591/150‑1 (MQ=255)
                                                                         gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  1:1688275/150‑1 (MQ=255)
                                                                           gTGGACTCGTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         >  1:1272231/1‑150 (MQ=255)
                                                                           gTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         <  2:130727/150‑1 (MQ=255)
                                                                            tGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAaga                                                                        <  2:706296/150‑1 (MQ=255)
                                                                                  cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCCTGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1280515/1‑150 (MQ=255)
                                                                                  cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  1:1081238/1‑150 (MQ=255)
                                                                                  cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1353234/1‑150 (MQ=255)
                                                                                   aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                 >  1:144299/1‑150 (MQ=255)
                                                                                   aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                 >  1:1714814/1‑150 (MQ=255)
                                                                                   aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAagaggagagg                                                                 >  2:1220714/1‑150 (MQ=255)
                                                                                     tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGCATAACCAGGCTATTTCTCCCTGCACAGGCCAAGTATGAAAGAGGAGAGGCt                                                               >  1:774450/1‑150 (MQ=255)
                                                                                     tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCt                                                               <  1:1553888/150‑1 (MQ=255)
                                                                                      cccGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCtt                                                              >  2:1590090/1‑150 (MQ=255)
                                                                                         gCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAg                                                           <  1:539994/150‑1 (MQ=255)
                                                                                            tGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTc                                                        >  1:624113/1‑150 (MQ=255)
                                                                                             gATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGCGGAGAGGCTTAAGTTCt                                                       >  1:224930/1‑150 (MQ=255)
                                                                                              aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:480511/77‑1 (MQ=255)
                                                                                              aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               >  2:480511/1‑77 (MQ=255)
                                                                                              aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCtt                                                      <  2:1356277/150‑1 (MQ=255)
                                                                                               tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:1072329/150‑1 (MQ=255)
                                                                                               tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     >  1:189430/1‑150 (MQ=255)
                                                                                               tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGAAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:314894/150‑1 (MQ=255)
                                                                                               tCCGTCATTAAATAAGTAACAGCACCCGCTGGTGTTTTCGTATGCACTGTCTATGCGAGAGACACTGCGATCTTAGCATGGGGCAGGGAATAAGCAGGCTTTTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCttt                                                     <  1:124347/150‑2 (MQ=255)
                                                                                                gcGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCTGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGGTCTTGa                                                    <  1:672587/149‑1 (MQ=255)
                                                                                                ccGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAAGAGGAGAGGCTTAAGTTCTTGa                                                    >  1:70139/1‑150 (MQ=255)
                                                                                                   tCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAcc                                                 <  1:1313461/150‑1 (MQ=255)
                                                                                                       tAAATAAGTAACCGCACCCTCGGGTGTTTTCGTATGCAGTGTCAATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             <  2:1393885/150‑1 (MQ=255)
                                                                                                       tAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             >  2:423879/1‑150 (MQ=255)
                                                                                                        aaaTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGaa                                            <  1:235942/150‑1 (MQ=255)
                                                                                                         aaTAAGTAACAGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGTGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAAc                                           <  1:264983/150‑1 (MQ=255)
                                                                                                          aaaactAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:410757/145‑1 (MQ=255)
                                                                                                          aTACGTAACAGCACCCACTTGTGTATTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:1682442/150‑1 (MQ=255)
                                                                                                          aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTTTGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          >  1:750208/1‑150 (MQ=255)
                                                                                                          aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:954859/150‑1 (MQ=255)
                                                                                                          aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  1:1672485/150‑1 (MQ=255)
                                                                                                                aaCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAc                                    >  1:665218/1‑150 (MQ=255)
                                                                                                                 aCAGCACCCTCTTGTGTTTTCGTATGAAGGGTCGATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   <  2:1342212/150‑1 (MQ=255)
                                                                                                                 aCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCTCAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   >  1:1591162/1‑150 (MQ=255)
                                                                                                                  cAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAATATGCAAGAGGAGAGGCTTAAGTACTTGAACCCTGAACGAATACCGa                                  >  1:1657929/1‑150 (MQ=255)
                                                                                                                    gCACCCTCTGGTGTTTTCGTATGCCGTGTCTATGCGACATACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACt                                <  2:334733/150‑1 (MQ=255)
                                                                                                                          tctGGTGTTTTCGGATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGa                          <  2:137113/150‑1 (MQ=255)
                                                                                                                           ctGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaa                         >  1:1322599/1‑150 (MQ=255)
                                                                                                                             ggTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATAAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaaaa                       >  1:561254/1‑150 (MQ=255)
                                                                                                                               tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     >  1:844312/1‑150 (MQ=255)
                                                                                                                               tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     <  1:1337490/150‑1 (MQ=255)
                                                                                                                                gtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCa                    >  2:1040655/1‑150 (MQ=255)
                                                                                                                                 ttttCGTATTCATTGTCTAAGCTACAGACACTGAGCTCTTATAATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGCAGAGGCATAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:170682/150‑1 (MQ=255)
                                                                                                                                 ttttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:1074706/150‑1 (MQ=255)
                                                                                                                                   ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGCCTACCAGAAAAGCACtt                 >  2:1689758/1‑150 (MQ=255)
                                                                                                                                   ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACtt                 >  2:1480947/1‑150 (MQ=255)
                                                                                                                                        gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATTACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGCACAGCACTTCGCAt            >  2:213233/3‑150 (MQ=255)
                                                                                                                                        gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCAt            >  2:724260/3‑150 (MQ=255)
                                                                                                                                         ttcAGTGTCTAAGCTACAGACACTGCGCTCATAGCATGGGGCAGGGAATAACCAGGCTATTTCTCGATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCATAAAAGCAATTCGCATc           <  2:1062649/148‑1 (MQ=255)
                                                                                                                                         tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:688263/1‑150 (MQ=255)
                                                                                                                                         tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           <  1:1040655/150‑1 (MQ=255)
                                                                                                                                         cgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:1112068/2‑150 (MQ=255)
                                                                                                                                          gCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCa          >  2:1795838/1‑150 (MQ=255)
                                                                                                                                           cAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAc         <  1:470947/150‑1 (MQ=255)
                                                                                                                                             gtgtCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTc       >  1:1214814/1‑150 (MQ=255)
                                                                                                                                               gtCTATGCGACAGACACTGCGCTCTTCCCATGGGGCAGTGAAAAACCAGGCTATAGCTCAATCCACAGCCCAATAATGAAAGAGGAGAGGCTCAAGTTCTAGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAg     <  1:284863/150‑1 (MQ=255)
                                                                                                                                                tCTATGTGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCAAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  1:498442/150‑1 (MQ=255)
                                                                                                                                                tCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  2:329022/150‑1 (MQ=255)
                                                                                                                                                gcTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  2:1212050/149‑1 (MQ=255)
                                                                                                                                                  tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAGTCAGACa  >  2:1438356/1‑150 (MQ=255)
                                                                                                                                                  tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa  <  1:208074/150‑1 (MQ=255)
                                                                                                                                                  tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa  >  1:1061855/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590452‑1590747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: