Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,590,602 C→G 8.5% intergenic (‑66/+252) yneL ← / ← hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,590,6020CG 8.5% 329.0 / 18.0 107intergenic (‑66/+252)yneL/hipApseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
Reads supporting (aligned to +/- strand):  ref base C (48/49);  new base G (5/4);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 108 total aligned reads displayed.

TGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590453‑1590747
                                                                                                                                                     |                                                                                                                                                 
tgtgGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGc                                                                                                                                                   >  1:970088/1‑150 (MQ=255)
 gtggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCg                                                                                                                                                  <  2:1213232/150‑1 (MQ=255)
 gtggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATACg                                                                                                                                                  <  2:1632557/150‑1 (MQ=255)
   ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                >  2:172229/1‑150 (MQ=255)
   ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                <  2:1370810/150‑1 (MQ=255)
      gACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgacaga                                                                                                                                             >  2:1672485/1‑150 (MQ=255)
      gACATAGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTTATCCGTCCTTAAATAAGTAACAGCACCCTATGGTGTTTTAGTATTCATTGTCAATGCtacaga                                                                                                                                             >  2:336842/1‑150 (MQ=255)
            gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGCCACTgc                                                                                                                                       >  1:1126333/1‑150 (MQ=255)
              aaTGTTCATTCAGCATCTGTCGCCTGCCGTCATAGAGACGTAGCCATTTCTGAGACCGGAGGGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTATCGTATGCAGTGGCTGTCGCATAGACACTgcat                                                                                                                                     <  1:310512/150‑3 (MQ=255)
               aTGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGGCCTTAAATAAGTAACAGCACCCGCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcata                                                                                                                                    <  1:777421/150‑4 (MQ=255)
                tGTTCATTCAGCATCTGTCGCCTGACTTCTTTGAGACGTAGCCATTTCTGATAGAGGAGTTGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcatac                                                                                                                                   <  1:1090145/150‑5 (MQ=255)
                tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                   >  1:329022/1‑150 (MQ=255)
                tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                   <  1:1092625/150‑1 (MQ=255)
                 gTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctct                                                                                                                                  >  1:337015/1‑150 (MQ=255)
                   tcattcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTa                                                                                                                                <  1:1141714/150‑1 (MQ=255)
                    cattcaGCATCTGTCGCCTGACTTCATTGAGTCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACTGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:623758/150‑1 (MQ=255)
                       tcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                                                            >  1:1103539/1‑150 (MQ=255)
                           cATCTGTCGCCTGACTTCATTGCGACGTAGCCTTTTCTGATCCCGGAATGTCCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTAAGCAGTGTCTATGCGACAGCCACTGGGCTCGTAGCATgggg                                                                                                                        >  2:1222840/1‑150 (MQ=255)
                              cTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAg                                                                                                                     >  2:1626094/1‑150 (MQ=255)
                               tGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAgg                                                                                                                    <  1:425902/150‑1 (MQ=255)
                                gTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGTCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAggg                                                                                                                   <  1:300380/150‑1 (MQ=255)
                                   gCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAAt                                                                                                                >  1:590887/1‑150 (MQ=255)
                                       gACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAAcc                                                                                                            >  1:451285/1‑150 (MQ=255)
                                         cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTATCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                          <  2:199036/150‑1 (MQ=255)
                                         cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                          <  1:89893/150‑1 (MQ=255)
                                           tCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGc                                                                                                        <  1:720111/150‑1 (MQ=255)
                                           tCATTGAGACGTAGACATTTCTGATACCGGAGTGGACTCATTCACGCTTGATCCGTACTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTTTGCAGTGTCTATGCGACAGACCCTGCGCTCTTAGCATGGGGCAGGGAATAACCACGc                                                                                                        >  2:79790/1‑150 (MQ=255)
                                                 agaCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtc                                                                                                  <  1:1478686/150‑1 (MQ=255)
                                                    cGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTcc                                                                                               <  2:337015/150‑1 (MQ=255)
                                                       aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:1748558/150‑1 (MQ=255)
                                                       aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGAAACTGAGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            >  2:136059/1‑150 (MQ=255)
                                                       aGCAATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:940077/150‑1 (MQ=255)
                                                        gCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGACTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTACCCAGGCTATTTCTCCAttc                                                                                           >  2:1092749/1‑148 (MQ=255)
                                                              tCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGTCAGACACTGCGCTCTTAGGATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGc                                                                                     >  2:345742/1‑150 (MQ=255)
                                                               cTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGcc                                                                                    <  1:1013376/150‑1 (MQ=255)
                                                                     ccGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTAt                                                                              >  1:847811/1‑150 (MQ=255)
                                                                        gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  1:1688275/150‑1 (MQ=255)
                                                                        gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  2:1523591/150‑1 (MQ=255)
                                                                          gTGGACTCGTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         >  1:1272231/1‑150 (MQ=255)
                                                                          gTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         <  2:130727/150‑1 (MQ=255)
                                                                           tGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAaga                                                                        <  2:706296/150‑1 (MQ=255)
                                                                             gACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagagg                                                                      >  2:1678712/1‑150 (MQ=255)
                                                                                 cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCCTGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1280515/1‑150 (MQ=255)
                                                                                 cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  1:1081238/1‑150 (MQ=255)
                                                                                 cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1353234/1‑150 (MQ=255)
                                                                                  aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                 >  1:144299/1‑150 (MQ=255)
                                                                                  aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAagaggagagg                                                                 >  2:1220714/1‑150 (MQ=255)
                                                                                    tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGCATAACCAGGCTATTTCTCCCTGCACAGGCCAAGTATGAAAGAGGAGAGGCt                                                               >  1:774450/1‑150 (MQ=255)
                                                                                    tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCt                                                               <  1:1553888/150‑1 (MQ=255)
                                                                                     cccGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCtt                                                              >  2:1590090/1‑150 (MQ=255)
                                                                                        gCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAg                                                           <  1:539994/150‑1 (MQ=255)
                                                                                           tGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTc                                                        >  1:624113/1‑150 (MQ=255)
                                                                                            gATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGCGGAGAGGCTTAAGTTCt                                                       >  1:224930/1‑150 (MQ=255)
                                                                                             aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               >  2:480511/1‑77 (MQ=255)
                                                                                             aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:480511/77‑1 (MQ=255)
                                                                                             aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCtt                                                      <  2:1356277/150‑1 (MQ=255)
                                                                                              tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:1072329/150‑1 (MQ=255)
                                                                                              tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     >  1:189430/1‑150 (MQ=255)
                                                                                              tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGAAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:314894/150‑1 (MQ=255)
                                                                                               gcGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCTGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGGTCTTGa                                                    <  1:672587/149‑1 (MQ=255)
                                                                                               ccGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAAGAGGAGAGGCTTAAGTTCTTGa                                                    >  1:70139/1‑150 (MQ=255)
                                                                                                  tCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAcc                                                 <  1:1313461/150‑1 (MQ=255)
                                                                                                      tAAATAAGTAACCGCACCCTCGGGTGTTTTCGTATGCAGTGTCAATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             <  2:1393885/150‑1 (MQ=255)
                                                                                                      tAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             >  2:423879/1‑150 (MQ=255)
                                                                                                       aaaTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGaa                                            <  1:235942/150‑1 (MQ=255)
                                                                                                        aaTAAGTAACAGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGTGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAAc                                           <  1:264983/150‑1 (MQ=255)
                                                                                                         aaaactAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:410757/145‑1 (MQ=255)
                                                                                                         aTACGTAACAGCACCCACTTGTGTATTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:1682442/150‑1 (MQ=255)
                                                                                                         aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTTTGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          >  1:750208/1‑150 (MQ=255)
                                                                                                         aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:954859/150‑1 (MQ=255)
                                                                                                         aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  1:1672485/150‑1 (MQ=255)
                                                                                                               aaCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAc                                    >  1:665218/1‑150 (MQ=255)
                                                                                                                aCAGCACCCTCTTGTGTTTTCGTATGAAGGGTCGATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   <  2:1342212/150‑1 (MQ=255)
                                                                                                                aCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCTCAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   >  1:1591162/1‑150 (MQ=255)
                                                                                                                 cAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAATATGCAAGAGGAGAGGCTTAAGTACTTGAACCCTGAACGAATACCGa                                  >  1:1657929/1‑150 (MQ=255)
                                                                                                                   gCACCCTCTGGTGTTTTCGTATGCCGTGTCTATGCGACATACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACt                                <  2:334733/150‑1 (MQ=255)
                                                                                                                         tctGGTGTTTTCGGATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGa                          <  2:137113/150‑1 (MQ=255)
                                                                                                                          ctGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaa                         >  1:1322599/1‑150 (MQ=255)
                                                                                                                            ggTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATAAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaaaa                       >  1:561254/1‑150 (MQ=255)
                                                                                                                              tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     >  1:844312/1‑150 (MQ=255)
                                                                                                                              tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     <  1:1337490/150‑1 (MQ=255)
                                                                                                                               gtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCa                    >  2:1040655/1‑150 (MQ=255)
                                                                                                                                ttttCGTATTCATTGTCTAAGCTACAGACACTGAGCTCTTATAATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGCAGAGGCATAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:170682/150‑1 (MQ=255)
                                                                                                                                ttttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:1074706/150‑1 (MQ=255)
                                                                                                                                  ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGCCTACCAGAAAAGCACtt                 >  2:1689758/1‑150 (MQ=255)
                                                                                                                                  ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACtt                 >  2:1480947/1‑150 (MQ=255)
                                                                                                                                       gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATTACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGCACAGCACTTCGCAt            >  2:213233/3‑150 (MQ=255)
                                                                                                                                       gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCAt            >  2:724260/3‑150 (MQ=255)
                                                                                                                                        ttcAGTGTCTAAGCTACAGACACTGCGCTCATAGCATGGGGCAGGGAATAACCAGGCTATTTCTCGATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCATAAAAGCAATTCGCATc           <  2:1062649/148‑1 (MQ=255)
                                                                                                                                        tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           <  1:1040655/150‑1 (MQ=255)
                                                                                                                                        tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:1813510/1‑150 (MQ=255)
                                                                                                                                        cgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:1112068/2‑150 (MQ=255)
                                                                                                                                         gCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCa          >  2:1795838/1‑150 (MQ=255)
                                                                                                                                          cAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAc         <  1:470947/150‑1 (MQ=255)
                                                                                                                                            gtgtCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTc       >  1:1214814/1‑150 (MQ=255)
                                                                                                                                              gtCTATGCGACAGACACTGCGCTCTTCCCATGGGGCAGTGAAAAACCAGGCTATAGCTCAATCCACAGCCCAATAATGAAAGAGGAGAGGCTCAAGTTCTAGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAg     <  1:284863/150‑1 (MQ=255)
                                                                                                                                               tCTATGTGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCAAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  1:498442/150‑1 (MQ=255)
                                                                                                                                               tCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  2:329022/150‑1 (MQ=255)
                                                                                                                                                 tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa  <  1:208074/150‑1 (MQ=255)
                                                                                                                                                 tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa  >  1:1061855/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590453‑1590747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: