Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,590,603 G→C 8.9% intergenic (‑67/+251) yneL ← / ← hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,590,6030GC 8.9% 305.8 / 18.2 101intergenic (‑67/+251)yneL/hipApseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
Reads supporting (aligned to +/- strand):  ref base G (45/45);  new base C (5/4);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 107 total aligned reads displayed.

GTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590454‑1590747
                                                                                                                                                     |                                                                                                                                                
gtggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCg                                                                                                                                                  <  2:1213232/150‑1 (MQ=255)
gtggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATACg                                                                                                                                                  <  2:1632557/150‑1 (MQ=255)
  ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                >  2:172229/1‑150 (MQ=255)
     gACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgacaga                                                                                                                                             >  2:1672485/1‑150 (MQ=255)
     gACATAGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTTATCCGTCCTTAAATAAGTAACAGCACCCTATGGTGTTTTAGTATTCATTGTCAATGCtacaga                                                                                                                                             >  2:336842/1‑150 (MQ=255)
           gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGATGTAGCCATTTATGATACCGGAGTGGACTCATTCCCTCTTGATCCGTCCTTAAATAAGTAACAGCACGCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgc                                                                                                                                       <  2:294173/150‑1 (MQ=255)
           gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGCCACTgc                                                                                                                                       >  1:1126333/1‑150 (MQ=255)
             aaTGTTCATTCAGCATCTGTCGCCTGCCGTCATAGAGACGTAGCCATTTCTGAGACCGGAGGGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTATCGTATGCAGTGGCTGTCGCATAGACACTgcat                                                                                                                                     <  1:310512/150‑3 (MQ=255)
              aTGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGGCCTTAAATAAGTAACAGCACCCGCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcata                                                                                                                                    <  1:777421/150‑4 (MQ=255)
               tGTTCATTCAGCATCTGTCGCCTGACTTCTTTGAGACGTAGCCATTTCTGATAGAGGAGTTGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcatac                                                                                                                                   <  1:1090145/150‑5 (MQ=255)
               tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                   >  1:329022/1‑150 (MQ=255)
               tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                   <  1:1092625/150‑1 (MQ=255)
                gTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctct                                                                                                                                  >  1:337015/1‑150 (MQ=255)
                  tcattcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTa                                                                                                                                <  1:1141714/150‑1 (MQ=255)
                   cattcaGCATCTGTCGCCTGACTTCATTGAGTCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACTGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:623758/150‑1 (MQ=255)
                      tcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                                                            >  1:1103539/1‑150 (MQ=255)
                        aGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGAGCTCTTAGCATgg                                                                                                                          >  1:363134/1‑150 (MQ=255)
                          cATCTGTCGCCTGACTTCATTGCGACGTAGCCTTTTCTGATCCCGGAATGTCCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTAAGCAGTGTCTATGCGACAGCCACTGGGCTCGTAGCATgggg                                                                                                                        >  2:1222840/1‑150 (MQ=255)
                             cTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAg                                                                                                                     >  2:1626094/1‑150 (MQ=255)
                              tGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAgg                                                                                                                    <  1:425902/150‑1 (MQ=255)
                               gTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGTCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAggg                                                                                                                   <  1:300380/150‑1 (MQ=255)
                                  gCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAAt                                                                                                                >  1:590887/1‑150 (MQ=255)
                                      gACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAAcc                                                                                                            >  1:451285/1‑150 (MQ=255)
                                          tCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGc                                                                                                        <  1:720111/150‑1 (MQ=255)
                                          tCATTGAGACGTAGACATTTCTGATACCGGAGTGGACTCATTCACGCTTGATCCGTACTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTTTGCAGTGTCTATGCGACAGACCCTGCGCTCTTAGCATGGGGCAGGGAATAACCACGc                                                                                                        >  2:79790/1‑150 (MQ=255)
                                                agaCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtc                                                                                                  <  1:1478686/150‑1 (MQ=255)
                                                   cGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTcc                                                                                               <  2:337015/150‑1 (MQ=255)
                                                      aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:1748558/150‑1 (MQ=255)
                                                      aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGAAACTGAGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            >  2:136059/1‑150 (MQ=255)
                                                      aGCAATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                            <  1:940077/150‑1 (MQ=255)
                                                       gCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGACTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTACCCAGGCTATTTCTCCAttc                                                                                           >  2:1092749/1‑148 (MQ=255)
                                                             tCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGTCAGACACTGCGCTCTTAGGATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGc                                                                                     >  2:345742/1‑150 (MQ=255)
                                                              cTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGcc                                                                                    <  1:1013376/150‑1 (MQ=255)
                                                                    ccGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTAt                                                                              >  1:847811/1‑150 (MQ=255)
                                                                       gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  2:1523591/150‑1 (MQ=255)
                                                                       gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                           <  1:1688275/150‑1 (MQ=255)
                                                                         gTGGACTCGTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         >  1:1272231/1‑150 (MQ=255)
                                                                         gTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                         <  2:130727/150‑1 (MQ=255)
                                                                          tGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAaga                                                                        <  2:706296/150‑1 (MQ=255)
                                                                            gACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagagg                                                                      >  2:1678712/1‑150 (MQ=255)
                                                                                cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCCTGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1280515/1‑150 (MQ=255)
                                                                                cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  1:1081238/1‑150 (MQ=255)
                                                                                cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                  >  2:1353234/1‑150 (MQ=255)
                                                                                 aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                 >  1:144299/1‑150 (MQ=255)
                                                                                 aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                 >  1:1714814/1‑150 (MQ=255)
                                                                                 aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAagaggagagg                                                                 >  2:1220714/1‑150 (MQ=255)
                                                                                   tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGCATAACCAGGCTATTTCTCCCTGCACAGGCCAAGTATGAAAGAGGAGAGGCt                                                               >  1:774450/1‑150 (MQ=255)
                                                                                   tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCt                                                               <  1:1553888/150‑1 (MQ=255)
                                                                                    cccGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCtt                                                              >  2:1590090/1‑150 (MQ=255)
                                                                                       gCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAg                                                           <  1:539994/150‑1 (MQ=255)
                                                                                          tGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTc                                                        >  1:624113/1‑150 (MQ=255)
                                                                                           gATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGCGGAGAGGCTTAAGTTCt                                                       >  1:224930/1‑150 (MQ=255)
                                                                                            aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               >  2:480511/1‑77 (MQ=255)
                                                                                            aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                               <  1:480511/77‑1 (MQ=255)
                                                                                            aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCtt                                                      <  2:1356277/150‑1 (MQ=255)
                                                                                             tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:1072329/150‑1 (MQ=255)
                                                                                             tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     >  1:189430/1‑150 (MQ=255)
                                                                                             tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGAAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                     <  1:314894/150‑1 (MQ=255)
                                                                                             tCCGTCATTAAATAAGTAACAGCACCCGCTGGTGTTTTCGTATGCACTGTCTATGCGAGAGACACTGCGATCTTAGCATGGGGCAGGGAATAAGCAGGCTTTTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCttt                                                     <  1:124347/150‑2 (MQ=255)
                                                                                              gcGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCTGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGGTCTTGa                                                    <  1:672587/149‑1 (MQ=255)
                                                                                              ccGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAAGAGGAGAGGCTTAAGTTCTTGa                                                    >  1:70139/1‑150 (MQ=255)
                                                                                                     tAAATAAGTAACCGCACCCTCGGGTGTTTTCGTATGCAGTGTCAATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             <  2:1393885/150‑1 (MQ=255)
                                                                                                     tAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                             >  2:423879/1‑150 (MQ=255)
                                                                                                      aaaTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGaa                                            <  1:235942/150‑1 (MQ=255)
                                                                                                       aaTAAGTAACAGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGTGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAAc                                           <  1:264983/150‑1 (MQ=255)
                                                                                                        aaaactAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:410757/145‑1 (MQ=255)
                                                                                                        aTACGTAACAGCACCCACTTGTGTATTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:1682442/150‑1 (MQ=255)
                                                                                                        aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTTTGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          >  1:750208/1‑150 (MQ=255)
                                                                                                        aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  2:954859/150‑1 (MQ=255)
                                                                                                        aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                          <  1:1672485/150‑1 (MQ=255)
                                                                                                              aaCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAc                                    >  1:665218/1‑150 (MQ=255)
                                                                                                               aCAGCACCCTCTTGTGTTTTCGTATGAAGGGTCGATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   <  2:1342212/150‑1 (MQ=255)
                                                                                                               aCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCTCAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                   >  1:1591162/1‑150 (MQ=255)
                                                                                                                cAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAATATGCAAGAGGAGAGGCTTAAGTACTTGAACCCTGAACGAATACCGa                                  >  1:1657929/1‑150 (MQ=255)
                                                                                                                  gCACCCTCTGGTGTTTTCGTATGCCGTGTCTATGCGACATACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACt                                <  2:334733/150‑1 (MQ=255)
                                                                                                                        tctGGTGTTTTCGGATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGa                          <  2:137113/150‑1 (MQ=255)
                                                                                                                         ctGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaa                         >  1:1322599/1‑150 (MQ=255)
                                                                                                                           ggTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATAAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaaaa                       >  1:561254/1‑150 (MQ=255)
                                                                                                                             tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     >  1:844312/1‑150 (MQ=255)
                                                                                                                             tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                     <  1:1337490/150‑1 (MQ=255)
                                                                                                                              gtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCa                    >  2:1040655/1‑150 (MQ=255)
                                                                                                                               ttttCGTATTCATTGTCTAAGCTACAGACACTGAGCTCTTATAATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGCAGAGGCATAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:170682/150‑1 (MQ=255)
                                                                                                                               ttttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                   <  1:1074706/150‑1 (MQ=255)
                                                                                                                                 ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGCCTACCAGAAAAGCACtt                 >  2:1689758/1‑150 (MQ=255)
                                                                                                                                 ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACtt                 >  2:1480947/1‑150 (MQ=255)
                                                                                                                                      gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATTACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGCACAGCACTTCGCAt            >  2:213233/3‑150 (MQ=255)
                                                                                                                                      gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCAt            >  2:724260/3‑150 (MQ=255)
                                                                                                                                       ttcAGTGTCTAAGCTACAGACACTGCGCTCATAGCATGGGGCAGGGAATAACCAGGCTATTTCTCGATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCATAAAAGCAATTCGCATc           <  2:1062649/148‑1 (MQ=255)
                                                                                                                                       tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:688263/1‑150 (MQ=255)
                                                                                                                                       tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:1813510/1‑150 (MQ=255)
                                                                                                                                       tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           <  1:1040655/150‑1 (MQ=255)
                                                                                                                                       cgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc           >  1:1112068/2‑150 (MQ=255)
                                                                                                                                        gCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCa          >  2:1795838/1‑150 (MQ=255)
                                                                                                                                         cAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAc         <  1:470947/150‑1 (MQ=255)
                                                                                                                                           gtgtCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTc       >  1:1214814/1‑150 (MQ=255)
                                                                                                                                             gtCTATGCGACAGACACTGCGCTCTTCCCATGGGGCAGTGAAAAACCAGGCTATAGCTCAATCCACAGCCCAATAATGAAAGAGGAGAGGCTCAAGTTCTAGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAg     <  1:284863/150‑1 (MQ=255)
                                                                                                                                              tCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  2:329022/150‑1 (MQ=255)
                                                                                                                                              gcTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa    <  2:1212050/149‑1 (MQ=255)
                                                                                                                                                tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAGTCAGACa  >  2:1438356/1‑150 (MQ=255)
                                                                                                                                                tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa  >  1:1061855/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACA  >  NC_000913/1590454‑1590747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: