Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,590,605 C→T 10.0% intergenic (‑69/+249) yneL ← / ← hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,590,6050CT10.0% 285.7 / 14.7 101intergenic (‑69/+249)yneL/hipApseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base T (5/5);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 109 total aligned reads displayed.

GGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTA  >  NC_000913/1590456‑1590754
                                                                                                                                                     |                                                                                                                                                     
ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                       <  2:1370810/150‑1 (MQ=255)
ggtgACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgac                                                                                                                                                       >  2:172229/1‑150 (MQ=255)
   gACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgacaga                                                                                                                                                    >  2:1672485/1‑150 (MQ=255)
   gACATAGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTTATCCGTCCTTAAATAAGTAACAGCACCCTATGGTGTTTTAGTATTCATTGTCAATGCtacaga                                                                                                                                                    >  2:336842/1‑150 (MQ=255)
         gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGATGTAGCCATTTATGATACCGGAGTGGACTCATTCCCTCTTGATCCGTCCTTAAATAAGTAACAGCACGCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgc                                                                                                                                              <  2:294173/150‑1 (MQ=255)
         gTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGCCACTgc                                                                                                                                              >  1:1126333/1‑150 (MQ=255)
           aaTGTTCATTCAGCATCTGTCGCCTGCCGTCATAGAGACGTAGCCATTTCTGAGACCGGAGGGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTATCGTATGCAGTGGCTGTCGCATAGACACTgcat                                                                                                                                            <  1:310512/150‑3 (MQ=255)
             tGTTCATTCAGCATCTGTCGCCTGACTTCTTTGAGACGTAGCCATTTCTGATAGAGGAGTTGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTGTCGCATAGACACTgcatac                                                                                                                                          <  1:1090145/150‑5 (MQ=255)
             tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                          >  1:329022/1‑150 (MQ=255)
             tGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctc                                                                                                                                          <  1:1092625/150‑1 (MQ=255)
              gTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctct                                                                                                                                         >  1:337015/1‑150 (MQ=255)
                tcattcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTa                                                                                                                                       <  1:1141714/150‑1 (MQ=255)
                 cattcaGCATCTGTCGCCTGACTTCATTGAGTCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACTGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                                      <  1:623758/150‑1 (MQ=255)
                    tcaGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                                                                   >  1:1103539/1‑150 (MQ=255)
                      aGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGAGCTCTTAGCATgg                                                                                                                                 >  1:363134/1‑150 (MQ=255)
                        cATCTGTCGCCTGACTTCATTGCGACGTAGCCTTTTCTGATCCCGGAATGTCCTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTAAGCAGTGTCTATGCGACAGCCACTGGGCTCGTAGCATgggg                                                                                                                               >  2:1222840/1‑150 (MQ=255)
                           cTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAg                                                                                                                            >  2:1626094/1‑150 (MQ=255)
                            tGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAgg                                                                                                                           <  1:425902/150‑1 (MQ=255)
                                gCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAAt                                                                                                                       >  1:590887/1‑150 (MQ=255)
                                    gACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAAcc                                                                                                                   >  1:451285/1‑150 (MQ=255)
                                      cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTATCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                                 <  2:199036/150‑1 (MQ=255)
                                      cTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAg                                                                                                                 <  1:89893/150‑1 (MQ=255)
                                        tCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGc                                                                                                               <  1:720111/150‑1 (MQ=255)
                                        tCATTGAGACGTAGACATTTCTGATACCGGAGTGGACTCATTCACGCTTGATCCGTACTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTTTGCAGTGTCTATGCGACAGACCCTGCGCTCTTAGCATGGGGCAGGGAATAACCACGc                                                                                                               >  2:79790/1‑150 (MQ=255)
                                              agaCGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtc                                                                                                         <  1:1478686/150‑1 (MQ=255)
                                                 cGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTcc                                                                                                      <  2:337015/150‑1 (MQ=255)
                                                    aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                                   <  1:1748558/150‑1 (MQ=255)
                                                    aGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGAAACTGAGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                                   >  2:136059/1‑150 (MQ=255)
                                                    aGCAATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGGGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATg                                                                                                   <  1:940077/150‑1 (MQ=255)
                                                     gCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGACTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTACCCAGGCTATTTCTCCAttc                                                                                                  >  2:1092749/1‑148 (MQ=255)
                                                           tCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTATATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGTCAGACACTGCGCTCTTAGGATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGc                                                                                            >  2:345742/1‑150 (MQ=255)
                                                            cTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGcc                                                                                           <  1:1013376/150‑1 (MQ=255)
                                                                  ccGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTAt                                                                                     >  1:847811/1‑150 (MQ=255)
                                                                     gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                                  <  1:1688275/150‑1 (MQ=255)
                                                                     gAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                                                                  <  2:1523591/150‑1 (MQ=255)
                                                                       gTGGACTCGTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                                >  1:1272231/1‑150 (MQ=255)
                                                                       gTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                                                                <  2:130727/150‑1 (MQ=255)
                                                                        tGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAaga                                                                               <  2:706296/150‑1 (MQ=255)
                                                                          gACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagagg                                                                             >  2:1678712/1‑150 (MQ=255)
                                                                              cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                         >  1:1081238/1‑150 (MQ=255)
                                                                              cATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagag                                                                         >  2:1353234/1‑150 (MQ=255)
                                                                               aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                        >  1:1714814/1‑150 (MQ=255)
                                                                               aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAagaggagagg                                                                        >  1:144299/1‑150 (MQ=255)
                                                                               aTTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAagaggagagg                                                                        >  2:1220714/1‑150 (MQ=255)
                                                                                 tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGCATAACCAGGCTATTTCTCCCTGCACAGGCCAAGTATGAAAGAGGAGAGGCt                                                                      >  1:774450/1‑150 (MQ=255)
                                                                                 tCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCt                                                                      <  1:1553888/150‑1 (MQ=255)
                                                                                        tGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTc                                                               >  1:624113/1‑150 (MQ=255)
                                                                                         gATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGCGGAGAGGCTTAAGTTCt                                                              >  1:224930/1‑150 (MQ=255)
                                                                                          aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                                      <  1:480511/77‑1 (MQ=255)
                                                                                          aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAg                                                                                                                                      >  2:480511/1‑77 (MQ=255)
                                                                                          aTCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCtt                                                             <  2:1356277/150‑1 (MQ=255)
                                                                                           tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                            <  1:1072329/150‑1 (MQ=255)
                                                                                           tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                            >  1:189430/1‑150 (MQ=255)
                                                                                           tCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGAAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTg                                                            <  1:314894/150‑1 (MQ=255)
                                                                                           tCCGTCATTAAATAAGTAACAGCACCCGCTGGTGTTTTCGTATGCACTGTCTATGCGAGAGACACTGCGATCTTAGCATGGGGCAGGGAATAAGCAGGCTTTTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCttt                                                            <  1:124347/150‑2 (MQ=255)
                                                                                            gcGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCTGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGGTCTTGa                                                           <  1:672587/149‑1 (MQ=255)
                                                                                            ccGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAGTATGAAAGAGGAGAGGCTTAAGTTCTTGa                                                           >  1:70139/1‑150 (MQ=255)
                                                                                               tCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAcc                                                        <  1:1313461/150‑1 (MQ=255)
                                                                                                   tAAATAAGTAACCGCACCCTCGGGTGTTTTCGTATGCAGTGTCAATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                                    <  2:1393885/150‑1 (MQ=255)
                                                                                                   tAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGa                                                    >  2:423879/1‑150 (MQ=255)
                                                                                                    aaaTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGaa                                                   <  1:235942/150‑1 (MQ=255)
                                                                                                     aaTAAGTAACAGCACCCTCTTGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGTGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAAc                                                  <  1:264983/150‑1 (MQ=255)
                                                                                                      aaaactAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                                 <  2:410757/145‑1 (MQ=255)
                                                                                                      aTACGTAACAGCACCCACTTGTGTATTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                                 <  2:1682442/150‑1 (MQ=255)
                                                                                                      aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTTTGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                                 >  1:750208/1‑150 (MQ=255)
                                                                                                      aTAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACg                                                 <  2:954859/150‑1 (MQ=255)
                                                                                                            aaCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAc                                           >  1:665218/1‑150 (MQ=255)
                                                                                                             aCAGCACCCTCTTGTGTTTTCGTATGAAGGGTCGATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                          <  2:1342212/150‑1 (MQ=255)
                                                                                                             aCAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCTCAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACg                                          >  1:1591162/1‑150 (MQ=255)
                                                                                                              cAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAATATGCAAGAGGAGAGGCTTAAGTACTTGAACCCTGAACGAATACCGa                                         >  1:1657929/1‑150 (MQ=255)
                                                                                                                      tctGGTGTTTTCGGATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGa                                 <  2:137113/150‑1 (MQ=255)
                                                                                                                       ctGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaa                                >  1:1322599/1‑150 (MQ=255)
                                                                                                                         ggTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATAAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGaaaa                              >  1:561254/1‑150 (MQ=255)
                                                                                                                           tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                            >  1:844312/1‑150 (MQ=255)
                                                                                                                           tgtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGc                            <  1:1337490/150‑1 (MQ=255)
                                                                                                                            gtTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCa                           >  2:1040655/1‑150 (MQ=255)
                                                                                                                             ttttCGTATTCATTGTCTAAGCTACAGACACTGAGCTCTTATAATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGCAGAGGCATAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                          <  1:170682/150‑1 (MQ=255)
                                                                                                                             ttttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCAc                          <  1:1074706/150‑1 (MQ=255)
                                                                                                                               ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGCCTACCAGAAAAGCACtt                        >  2:1689758/1‑150 (MQ=255)
                                                                                                                               ttCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACtt                        >  2:1480947/1‑150 (MQ=255)
                                                                                                                                    gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATTACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGCACAGCACTTCGCAt                   >  2:213233/3‑150 (MQ=255)
                                                                                                                                    gcgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCAt                   >  2:724260/3‑150 (MQ=255)
                                                                                                                                     ttcAGTGTCTAAGCTACAGACACTGCGCTCATAGCATGGGGCAGGGAATAACCAGGCTATTTCTCGATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCATAAAAGCAATTCGCATc                  <  2:1062649/148‑1 (MQ=255)
                                                                                                                                     tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc                  <  1:1040655/150‑1 (MQ=255)
                                                                                                                                     tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc                  >  1:1813510/1‑150 (MQ=255)
                                                                                                                                     tGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc                  >  1:688263/1‑150 (MQ=255)
                                                                                                                                     cgCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATc                  >  1:1112068/2‑150 (MQ=255)
                                                                                                                                      gCAGTGTCTGTCGCATAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCa                 >  2:1795838/1‑150 (MQ=255)
                                                                                                                                       cAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAc                <  1:470947/150‑1 (MQ=255)
                                                                                                                                           gtCTATGCGACAGACACTGCGCTCTTCCCATGGGGCAGTGAAAAACCAGGCTATAGCTCAATCCACAGCCCAATAATGAAAGAGGAGAGGCTCAAGTTCTAGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAg            <  1:284863/150‑1 (MQ=255)
                                                                                                                                            tCTATGTGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCAAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa           <  1:498442/150‑1 (MQ=255)
                                                                                                                                            tCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa           <  2:329022/150‑1 (MQ=255)
                                                                                                                                            gcTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCACCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGa           <  2:1212050/149‑1 (MQ=255)
                                                                                                                                              tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCAGTCAGACa         >  2:1438356/1‑150 (MQ=255)
                                                                                                                                              tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa         >  1:1061855/1‑150 (MQ=255)
                                                                                                                                              tATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACa         <  1:208074/150‑1 (MQ=255)
                                                                                                                                                    acagacaCTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGAttt   <  1:533103/150‑1 (MQ=255)
                                                                                                                                                    acagacaCTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGAttt   <  2:847811/150‑1 (MQ=255)
                                                                                                                                                     cagacaCTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTa  <  1:1353234/150‑1 (MQ=255)
                                                                                                                                                     cagacaCTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTa  <  2:1126333/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTA  >  NC_000913/1590456‑1590754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: