Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,620,227 C→G 5.7% intergenic (+20/+11) marB → / ← eamA periplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,620,2270CG 5.7% 400.4 / 12.3 122intergenic (+20/+11)marB/eamAperiplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base C (41/74);  new base G (3/4);  total (44/78)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 125 total aligned reads displayed.

TTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620078‑1620375
                                                                                                                                                     |                                                                                                                                                    
 tcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtct                                                                                                                                                     >  1:703696/1‑150 (MQ=255)
    tgtgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCCCCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCgg                                                                                                                                                  >  1:705670/1‑150 (MQ=255)
     gtgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCGATTTAGTTTGTTCTGGCCCCGACATCTAggg                                                                                                                                                 >  2:502189/1‑150 (MQ=255)
     gtgCCAATGTCGGGGTTGTTCCCCCATCGCAGGAACACCCACCGGTTGATTTAAATCACATGGGTCCTGGCAGTGATCAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCggg                                                                                                                                                 <  1:31958/150‑1 (MQ=255)
      ggCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGTTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCgggg                                                                                                                                                <  1:1176413/149‑1 (MQ=255)
       gCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCggggg                                                                                                                                               >  1:1399721/1‑149 (MQ=255)
         cAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCATCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCGCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcca                                                                                                                                             <  1:332263/150‑3 (MQ=255)
         cAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcca                                                                                                                                             <  2:6001/150‑3 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCtta                                                                                                                                            >  1:469407/1‑150 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCtta                                                                                                                                            >  1:427617/1‑150 (MQ=255)
           aTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccaga                                                                                                                                           <  2:944828/150‑5 (MQ=255)
             gTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGGTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccagaac                                                                                                                                         <  1:456916/150‑7 (MQ=255)
                ttGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACt                                                                                                                                      <  1:1000113/149‑1 (MQ=255)
                gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACt                                                                                                                                      <  2:229546/150‑1 (MQ=255)
                  ggTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                                                                    <  1:1620201/150‑1 (MQ=255)
                   gttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                                                                   <  2:52864/150‑1 (MQ=255)
                   gttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                                                                   <  1:1074950/150‑1 (MQ=255)
                    ttgttCCCCCATAGACGGAACACCCACCGTTTGATGTAAATCACATGGGTACTGGCAGTGATAAGTCGGAAGCCCGCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                                                                  <  2:323773/150‑1 (MQ=255)
                      gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCTGATGCGCTCGGCGTGCCCTATTATAATCAACCCGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAc                                                                                                                                <  2:278519/150‑1 (MQ=255)
                      gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAc                                                                                                                                <  2:47073/150‑1 (MQ=255)
                      gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAc                                                                                                                                <  2:1730678/150‑1 (MQ=255)
                           cccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCCCCTTTa                                                                                                                           >  1:515260/1‑150 (MQ=255)
                            ccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                                                          <  2:461435/150‑1 (MQ=255)
                            ccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                                                          >  2:139659/1‑150 (MQ=255)
                             cATCGCAGGAACACCCACAGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAcc                                                                                                                         <  2:549396/150‑1 (MQ=255)
                               tCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGc                                                                                                                       <  2:769856/150‑1 (MQ=255)
                                 gCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCtt                                                                                                                     >  1:943995/1‑150 (MQ=255)
                                 gCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGAGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGCCATCTCGGCGCTTATTAACTTCCCACCTTTACCGCtt                                                                                                                     >  2:1516344/1‑150 (MQ=255)
                                   aGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTa                                                                                                                   <  2:1000767/150‑1 (MQ=255)
                                    ggAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAc                                                                                                                  <  1:1039065/150‑1 (MQ=255)
                                        cacccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCa                                                                                                              <  2:931839/150‑1 (MQ=255)
                                         acccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAc                                                                                                             <  2:1782008/150‑1 (MQ=255)
                                          cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAcc                                                                                                            >  1:1402489/1‑150 (MQ=255)
                                                     aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAAt                                                                                                 >  1:186979/1‑150 (MQ=255)
                                                     aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGAAGGTTGTTCAGGCCCCGACATCTCGGGGCTTATTTACTTCCCACCTTTACCGCTTTCCTCCACCGCAAGCCAAAt                                                                                                 >  2:264312/1‑150 (MQ=255)
                                                          aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATACTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAtt                                                                                            <  2:624681/150‑1 (MQ=255)
                                                          aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAtt                                                                                            >  1:1022899/1‑150 (MQ=255)
                                                          aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAtt                                                                                            <  1:1329524/150‑1 (MQ=255)
                                                           aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                           >  1:736493/1‑150 (MQ=255)
                                                             cacaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGat                                                                                         <  2:945841/150‑1 (MQ=255)
                                                             cacaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGat                                                                                         <  2:1535643/150‑1 (MQ=255)
                                                             cacaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGat                                                                                         <  2:661931/150‑1 (MQ=255)
                                                                  gggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACa                                                                                    >  2:1374853/1‑150 (MQ=255)
                                                                  gggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACa                                                                                    <  2:747454/150‑1 (MQ=255)
                                                                   ggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACac                                                                                   >  2:545050/1‑149 (MQ=255)
                                                                   ggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAg                                                                                   <  1:1776806/150‑1 (MQ=255)
                                                                      aCTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGccc                                                                                <  1:890966/150‑1 (MQ=255)
                                                                         ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGt                                                                             <  2:1676925/150‑1 (MQ=255)
                                                                         ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGAAAGCCAAATACATTGATATACAGCCCGGt                                                                             >  1:1567627/1‑150 (MQ=255)
                                                                          gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                                                            <  2:35637/150‑1 (MQ=255)
                                                                           cAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGTCACCGCCATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAATGATAAACAGCCCGGTCa                                                                           >  2:758330/1‑150 (MQ=255)
                                                                           cAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCa                                                                           >  2:650269/1‑150 (MQ=255)
                                                                             gTGATAAGTCGGAAGCGCTCGGCGTCCCGTATTTTAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTAATAACTACGCACCTTTCCCGCTTTACGCCAACGCAAGCCAAATACATTGATATACAGCCCGGACATa                                                                         >  2:1288044/1‑150 (MQ=255)
                                                                               gATAAGGCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                                                                       <  1:1806771/150‑1 (MQ=255)
                                                                                   aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgc                                                                   <  2:1669483/150‑1 (MQ=255)
                                                                                   aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgc                                                                   <  2:856554/150‑1 (MQ=255)
                                                                                    gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgca                                                                  <  2:705670/150‑1 (MQ=255)
                                                                                    gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgca                                                                  >  1:782910/1‑150 (MQ=255)
                                                                                       ggATGCGCTCGTCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGTTGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccg                                                               <  1:279411/150‑1 (MQ=255)
                                                                                          tGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcac                                                            >  2:1820797/1‑150 (MQ=255)
                                                                                           gcgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcacc                                                           <  2:810878/150‑1 (MQ=255)
                                                                                            cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCt                                                          >  2:972691/1‑150 (MQ=255)
                                                                                                cGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTaaaa                                                      <  2:943995/150‑1 (MQ=255)
                                                                                                 ggCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTTTTAACTTCCCTCCTTTACCGCTTTACGCCAGCGCAAGCCAAATACTTTGATATACAGCGCGGTCATAATGAGCACCGCACCTaaaaa                                                     <  2:611907/150‑1 (MQ=255)
                                                                                                   cGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAAtt                                                   >  1:1477129/1‑150 (MQ=255)
                                                                                                   cGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACATCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAAtt                                                   <  1:452903/150‑1 (MQ=255)
                                                                                                    gTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTg                                                  >  2:888644/1‑150 (MQ=255)
                                                                                                    gTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTg                                                  <  1:365653/150‑1 (MQ=255)
                                                                                                    gTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTg                                                  <  2:364167/150‑1 (MQ=255)
                                                                                                     tgaccTATTATAATCATCACGCTATGTAGTTTGTTCTGGCACCGACATCTCGGGGCTTATTATCTTCCCACCTATACCTCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGc                                                 <  1:115431/147‑1 (MQ=255)
                                                                                                     tGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGc                                                 <  1:2272/150‑1 (MQ=255)
                                                                                                     gtcccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGc                                                 <  2:265604/148‑1 (MQ=255)
                                                                                                      gCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa                                                <  2:1384059/150‑1 (MQ=255)
                                                                                                       cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg                                               <  2:427617/150‑1 (MQ=255)
                                                                                                           attatAATCAACACGTTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCAAAATACTATGATATACAGCCGGGGCATAATGAGCTCCGCTCCTAAAAATTGCAGAccc                                           >  1:745430/1‑150 (MQ=255)
                                                                                                                aaTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTaa                                      >  2:1620396/1‑150 (MQ=255)
                                                                                                                aaTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTaa                                      >  2:1759326/1‑150 (MQ=255)
                                                                                                                   cAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCg                                   <  1:1247480/150‑1 (MQ=255)
                                                                                                                     acacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGtt                                 <  2:1085496/150‑1 (MQ=255)
                                                                                                                        cGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGGTATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTTCAGACCCGTTAAGCGTTCAt                              >  2:1122402/1‑150 (MQ=255)
                                                                                                                         gCTATGTAGTTTGTTCTGGCCCCGACATCTCGTGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATc                             <  2:703696/150‑1 (MQ=255)
                                                                                                                          cTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATcc                            <  2:1177879/150‑1 (MQ=255)
                                                                                                                          cTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATcc                            <  1:139659/150‑1 (MQ=255)
                                                                                                                          atATGTAGTTTGTTCTTGCCCCGAAATCTCGTGGCTTATTAACTTCCCACATTTACCGCTTTACGCCAACGCAAGCAAAGTACATTGATATACAGCCCGGTCATAATGAGCAACGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATcc                            <  1:121147/149‑1 (MQ=255)
                                                                                                                           tATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCca                           >  2:468708/1‑150 (MQ=255)
                                                                                                                             tGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         <  2:853710/150‑1 (MQ=255)
                                                                                                                             tGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         <  1:732465/150‑1 (MQ=255)
                                                                                                                             tGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         >  1:910382/1‑150 (MQ=255)
                                                                                                                             tGTAGTTTGTGCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         <  1:362326/150‑1 (MQ=255)
                                                                                                                                 gTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCATATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATa                     >  1:230637/1‑150 (MQ=255)
                                                                                                                                   ttGTTCTGGAGCAGACATCTCGGGGCTTATTTACTTCCCACCTTTAGCGCTTTACGCCACCGCAAGCCAAATACATTGAATTACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCTGACCCGATAAGCGTTCATCCAACAATAGt                   <  2:1131183/150‑1 (MQ=255)
                                                                                                                                     gTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAAGAGCACCGCACCTAAAAATTGCCGCCCCGTTTAGCGTTCATCCAACAATAGTGc                 >  1:1312925/1‑150 (MQ=255)
                                                                                                                                      aataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGcc                >  2:1817096/6‑150 (MQ=255)
                                                                                                                                       ataagCCCCGAGATGTCGGGGCTTATTAACTTCCCTCCTTTACCGCTTTACGCCCCCGCAAGCCAAATACATTGATATACCGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCg               >  2:680914/5‑150 (MQ=255)
                                                                                                                                          agCCCCGAGATGTCGGGGCTTATTAACTTCCCACCAATACCGCTTTACGCCACCGCCAGCCGAATACATTGATATACAGCCCGGTCATAAAGAGCTCCGCACCTAAAAATTGCAGACCCGTTTTGCGTTGAGCCATCAAGAGTGCAGCAc            >  2:179788/2‑150 (MQ=255)
                                                                                                                                           gCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTGATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACt           <  2:186979/150‑1 (MQ=255)
                                                                                                                                              ccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGc        <  2:469407/150‑1 (MQ=255)
                                                                                                                                               cGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGcc       <  1:1374853/150‑1 (MQ=255)
                                                                                                                                                   aTCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTc   <  1:1271132/150‑1 (MQ=255)
                                                                                                                                                    tctcGGGGCTTATTCACTTCCCACCTTTACCGCTTTCCGCCACCGCAAGCCAAATACATTGATCTACAGCCCGGTCCTACGGCGCTCCGCACCTAAAACTTGCCGACCCGTTAAGCGTTCATCCAACAATCGTGCCGCACTTGCCAGTcc  <  1:60423/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620078‑1620375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: