Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,643,111 T→G 5.7% K44T (AAA→ACA) ‡ quuQ ← Qin prophage; putative antitermination protein Q

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,643,1110TG 5.7% 306.4 / 6.3 99K44T (AAA→ACA) ‡quuQQin prophage; putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base G (4/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 102 total aligned reads displayed.

AAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGC  >  NC_000913/1642962‑1643260
                                                                                                                                                     |                                                                                                                                                     
aaGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTTGCAGGCCTTCAATCGCAAACTGAGGGTCCTGACCCCCCACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCtt                                                                                                                                                       >  2:910225/1‑150 (MQ=255)
 aGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCttt                                                                                                                                                      <  2:543467/150‑1 (MQ=255)
   gAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCg                                                                                                                                                    <  1:1012789/150‑1 (MQ=255)
   gAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCg                                                                                                                                                    <  1:1097026/150‑1 (MQ=255)
     ataatCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGAc                                                                                                                                                  <  2:1420946/150‑1 (MQ=255)
     ataatCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGAc                                                                                                                                                  <  1:185594/150‑1 (MQ=255)
      taatCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACt                                                                                                                                                 <  1:769390/150‑1 (MQ=255)
           tGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCAcc                                                                                                                                            >  2:539715/1‑150 (MQ=255)
                 cGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCAGTCGAAAGCACCacttgg                                                                                                                                      <  2:827206/150‑6 (MQ=255)
                  ggATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCt                                                                                                                                     >  2:27350/1‑150 (MQ=255)
                  ggATTTCTTCACTCAATTTGTTCAATGACGGGTTATCCAGTGCAATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCAGTCGAAAGCACCacttggg                                                                                                                                     <  1:491307/150‑7 (MQ=255)
                   gATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTg                                                                                                                                    <  2:1470227/150‑1 (MQ=255)
                    aTTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgc                                                                                                                                   <  2:878048/150‑1 (MQ=255)
                     tttCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgcg                                                                                                                                  <  1:1487854/150‑1 (MQ=255)
                     tttCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgcg                                                                                                                                  <  1:740348/150‑1 (MQ=255)
                        cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                               <  2:1395001/150‑1 (MQ=255)
                        cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                               <  2:1107836/150‑1 (MQ=255)
                        cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                               >  2:427340/1‑150 (MQ=255)
                          tcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGACCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGcc                                                                                                                             <  2:210156/150‑1 (MQ=255)
                           cACTCAATTTGTTCATTGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCTATCGCAAACTGAGGGTCGTGATAACCAACTTTTGCCAGTAATAAGGCTAACCCAAGTGGTGCTTTCGACTGCAGCCTCCCCTGCGCTGCCa                                                                                                                            >  2:831364/1‑150 (MQ=255)
                            aCTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAt                                                                                                                           >  1:979951/1‑150 (MQ=255)
                             cTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAtt                                                                                                                          <  2:1791562/150‑1 (MQ=255)
                             cTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAtt                                                                                                                          >  1:1654399/1‑150 (MQ=255)
                              tCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTa                                                                                                                         <  2:1727219/150‑1 (MQ=255)
                              tCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTa                                                                                                                         <  2:64696/150‑1 (MQ=255)
                               cAATTTGTTCAATGTCGGGTTATCCAGTGCCATCTCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTAc                                                                                                                        <  1:1235533/150‑1 (MQ=255)
                                   ttGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCGTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATcc                                                                                                                    <  2:1581398/150‑1 (MQ=255)
                                    tGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCg                                                                                                                   <  1:796240/150‑1 (MQ=255)
                                     gTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGt                                                                                                                  <  1:1279491/150‑1 (MQ=255)
                                        cAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGACAACCCAAGTGGTGCTTTCGCCTGCACCATCCCCTGCGCTGCCATTACATCCGTAAt                                                                                                               >  1:981140/1‑150 (MQ=255)
                                            gTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGtt                                                                                                           <  2:205543/150‑1 (MQ=255)
                                             acGGGTTATCCAGTGCAATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTACTGAACACCAACTTTTGCCAGAAATAGGGCCAACCCAAGAGCTACTTACGACTGCACCATCCCCAGCGCGGCCATTACATCCGTAATAGTTa                                                                                                          >  2:286058/2‑150 (MQ=255)
                                                ggTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCCCCCTCCCCTGCGCTGCCATTACATCCGTAATTGTTAaac                                                                                                       >  2:1810066/1‑150 (MQ=255)
                                                     tCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCg                                                                                                  <  2:1494681/150‑1 (MQ=255)
                                                      ccAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGTCCAACCCAAGTGGTGCGTTCGACTGCAAAATCCCCTGCGCTGCCAATACATCCGTAATTGTTAAACAACCgg                                                                                                 >  1:286411/1‑150 (MQ=255)
                                                       cAGTGCCATCGAGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCCTCCCCTGCGCTGCCATTACATCCGGAATTGTTAAACACCCGGt                                                                                                >  1:162683/1‑150 (MQ=255)
                                                          tGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGcc                                                                                             <  1:258216/150‑1 (MQ=255)
                                                           gCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGCGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCACCCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTACACAACCGGTGCCt                                                                                            >  1:1048813/1‑150 (MQ=255)
                                                           gCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGATAAACAACCGGTGCCt                                                                                            >  2:628146/1‑150 (MQ=255)
                                                            ccATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTg                                                                                           >  2:845032/1‑150 (MQ=255)
                                                            ccATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTg                                                                                           <  2:1721221/150‑1 (MQ=255)
                                                              aTCGCGTAATTTAGCAGGCCTTCAATAGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCGTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTc                                                                                         >  1:653024/1‑150 (MQ=255)
                                                               tCGCGTATTTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTTAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCg                                                                                        <  1:823920/150‑1 (MQ=255)
                                                                cgcgTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCAGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGCCTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGACGc                                                                                       >  2:693409/1‑150 (MQ=255)
                                                                 gcgTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAACCAACCGGTGCCTGTCGCt                                                                                      >  2:1311444/1‑150 (MQ=255)
                                                                    tAATTTAGCAGGCCTTCAATCGCAAACTGCGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGa                                                                                   >  1:1221250/1‑150 (MQ=255)
                                                                     aaTTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAg                                                                                  >  1:32247/1‑150 (MQ=255)
                                                                       tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                <  2:1654399/150‑1 (MQ=255)
                                                                       tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                >  2:963446/1‑150 (MQ=255)
                                                                       tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                <  1:1131219/150‑1 (MQ=255)
                                                                          aGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGTAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCCCCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGTCGCTGGAGCGTCa                                                                             >  2:877723/1‑150 (MQ=255)
                                                                              ggCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGc                                                                         >  2:1698155/1‑150 (MQ=255)
                                                                                ccTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGTCGCTGGAGCGTCATCGCTc                                                                       >  2:561880/1‑150 (MQ=255)
                                                                                ccTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTc                                                                       >  2:618336/1‑150 (MQ=255)
                                                                                     aaTCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAAttt                                                                  >  2:634946/1‑150 (MQ=255)
                                                                                      aTCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAAtttt                                                                 >  2:1038432/1‑150 (MQ=255)
                                                                                       tcggtaaCTGAGGGTGCTTAACACCAACTTTTGCCAGAAATTAGGCCAACCGAAGTGGTGCTTTCGACTGCTCCATCCCCTGCGCTGCGATTAGATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGATCAATTTTg                                                                <  2:1310161/145‑1 (MQ=255)
                                                                                         gCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGga                                                              <  1:1166951/150‑1 (MQ=255)
                                                                                          cAACCTGAGCGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGgag                                                             <  2:365842/150‑1 (MQ=255)
                                                                                            aaCTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGAt                                                           >  2:275341/1‑150 (MQ=255)
                                                                                                  gggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGATTTCGACTGCACCATCCCCTGCGCTGCCATTAAATCCGTAAATGTTAAACAACCTGTGCCTGTCGCTGGAGCGTCAGCGATAAAGTTTGGAGATGTTggg                                                     >  2:1221455/1‑150 (MQ=255)
                                                                                                   ggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTgggg                                                    <  1:1775323/150‑1 (MQ=255)
                                                                                                   ggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTgggg                                                    >  2:1085183/1‑150 (MQ=255)
                                                                                                     tCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAg                                                  <  1:1595247/150‑1 (MQ=255)
                                                                                                      ccTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGt                                                 <  1:539715/150‑1 (MQ=255)
                                                                                                       cTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTa                                                >  1:184170/1‑150 (MQ=255)
                                                                                                       cTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTa                                                >  1:1676478/1‑150 (MQ=255)
                                                                                                        tGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTaa                                               >  2:1256453/1‑150 (MQ=255)
                                                                                                         taaCACCAACTTTTGCCAGAAATAAGGCCAACACAAGAGGTGCTTTCGACTGCACCATCACCTGCGCTGCCATTACCTCGGTAATTTTTAAACACACCGTGCCTGTCGCTGGAGCGTCATCGCTCAATTATCGAGATTTTGGGCAGTaaa                                              >  2:556050/2‑149 (MQ=255)
                                                                                                           acacCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGGTTTCGACTGCTCCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAatat                                            <  2:449193/150‑1 (MQ=255)
                                                                                                           acacCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATATTGGAGATTTTGGGGAGTAatat                                            >  2:535094/1‑150 (MQ=255)
                                                                                                               cAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTg                                        <  2:1819343/150‑1 (MQ=255)
                                                                                                                aaCTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                       <  2:1309881/150‑1 (MQ=255)
                                                                                                                aaCTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                       <  2:32247/150‑1 (MQ=255)
                                                                                                                aaCTGTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCGATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCGGGAGCATCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                       <  2:1649460/150‑1 (MQ=255)
                                                                                                                  cTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTa                                     <  1:1038432/150‑1 (MQ=255)
                                                                                                                       gCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATATTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCt                                >  2:544760/1‑150 (MQ=255)
                                                                                                                        ccAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCtt                               >  1:582596/1‑150 (MQ=255)
                                                                                                                         cAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTc                              >  2:1080104/1‑150 (MQ=255)
                                                                                                                          aGAACTACGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGGCATCGCTCAATTATGGAGATTTAGGGGAGTAATATTTTGGTAAGGCTTCa                             <  2:302048/150‑1 (MQ=255)
                                                                                                                          aGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAACCAACCGGTGCCTGTCGCTGGAGCGTCTTCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCa                             >  1:1603595/1‑150 (MQ=255)
                                                                                                                             aaTAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAgg                          <  1:217714/150‑1 (MQ=255)
                                                                                                                                  tgCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCGGGCATTACATCCGTAATTGTTAAACAACCGGTTCCTGTCGCTGGAGAGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                     <  1:111622/149‑1 (MQ=255)
                                                                                                                                  ggCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                     >  2:1720894/1‑150 (MQ=255)
                                                                                                                                  ggCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                     <  1:6404/150‑1 (MQ=255)
                                                                                                                                    ccAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCTCTGCCATTACATCCGTAATTGTTAAACAACCGGTGAGTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTTATATTTTGGTAAGGCTTCAAGGTTCATGc                   <  1:5165/150‑1 (MQ=255)
                                                                                                                                    ccAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGc                   >  2:477948/1‑150 (MQ=255)
                                                                                                                                     cAACCCAAGTGGTGCTATCGACTGCACCATCCCCTGCGCTGCCATTACCTCCGTAATTGATAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGAATTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCt                  >  2:819947/1‑150 (MQ=255)
                                                                                                                                       aCCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCg                <  1:1070165/150‑1 (MQ=255)
                                                                                                                                        cccAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTTCATCCGTAATTGTTAAACAACCGGTGCCTCTCGCTGGAGCGTAATCAATCAAATTTGTAGATTTTGTGTAGTAATATTTTGTTAAGGCTTCAAGGTTCATGCTAGt               >  2:1204565/1‑150 (MQ=255)
                                                                                                                                          cAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtc             <  1:1222785/150‑1 (MQ=255)
                                                                                                                                           ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtct            >  2:818756/4‑150 (MQ=255)
                                                                                                                                           ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtct            >  1:1258742/4‑150 (MQ=255)
                                                                                                                                           ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc           >  1:747233/4‑150 (MQ=255)
                                                                                                                                            gatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc           >  2:395168/3‑150 (MQ=255)
                                                                                                                                            aGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc           <  1:27350/150‑1 (MQ=255)
                                                                                                                                               ggtgCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTTATAGTTTGGGAAGGCGTCATGGTTCATGCTCGTTCTCCAc        >  1:274185/1‑150 (MQ=255)
                                                                                                                                                gtgCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACt       >  1:340053/1‑150 (MQ=255)
                                                                                                                                                  gCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCGCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTa     <  1:555557/150‑1 (MQ=255)
                                                                                                                                                     ttCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGc  >  2:1745429/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGC  >  NC_000913/1642962‑1643260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: