Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,643,117 T→A 6.1% Q42L (CAG→CTG) ‡ quuQ ← Qin prophage; putative antitermination protein Q

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,643,1170TA 6.1% 290.1 / 7.0 98Q42L (CAG→CTG) ‡quuQQin prophage; putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base T (45/47);  new base A (4/2);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGCC  >  NC_000913/1642968‑1643261
                                                                                                                                                     |                                                                                                                                                
taatCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACt                                                                                                                                                  <  1:769390/150‑1 (MQ=255)
     tGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCAcc                                                                                                                                             >  2:539715/1‑150 (MQ=255)
           cGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCAGTCGAAAGCACCacttgg                                                                                                                                       <  2:827206/150‑6 (MQ=255)
            ggATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCt                                                                                                                                      >  2:27350/1‑150 (MQ=255)
            ggATTTCTTCACTCAATTTGTTCAATGACGGGTTATCCAGTGCAATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCAGTCGAAAGCACCacttggg                                                                                                                                      <  1:491307/150‑7 (MQ=255)
             gATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTg                                                                                                                                     <  2:1470227/150‑1 (MQ=255)
              aTTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgc                                                                                                                                    <  2:878048/150‑1 (MQ=255)
               tttCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgcg                                                                                                                                   <  1:740348/150‑1 (MQ=255)
               tttCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTgcg                                                                                                                                   <  1:1487854/150‑1 (MQ=255)
                  cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                                <  2:1107836/150‑1 (MQ=255)
                  cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                                <  2:1395001/150‑1 (MQ=255)
                  cttcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTg                                                                                                                                >  2:427340/1‑150 (MQ=255)
                    tcACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGACCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGcc                                                                                                                              <  2:210156/150‑1 (MQ=255)
                     cACTCAATTTGTTCATTGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCTATCGCAAACTGAGGGTCGTGATAACCAACTTTTGCCAGTAATAAGGCTAACCCAAGTGGTGCTTTCGACTGCAGCCTCCCCTGCGCTGCCa                                                                                                                             >  2:831364/1‑150 (MQ=255)
                      aCTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAt                                                                                                                            >  1:979951/1‑150 (MQ=255)
                       cTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAtt                                                                                                                           >  1:1654399/1‑150 (MQ=255)
                       cTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCAtt                                                                                                                           <  2:1791562/150‑1 (MQ=255)
                        tCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTa                                                                                                                          <  2:64696/150‑1 (MQ=255)
                        tCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTa                                                                                                                          <  2:1727219/150‑1 (MQ=255)
                         cAATTTGTTCAATGTCGGGTTATCCAGTGCCATCTCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTAc                                                                                                                         <  1:1235533/150‑1 (MQ=255)
                             ttGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCGTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATcc                                                                                                                     <  2:1581398/150‑1 (MQ=255)
                              tGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCg                                                                                                                    <  1:796240/150‑1 (MQ=255)
                               gTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGt                                                                                                                   <  1:1279491/150‑1 (MQ=255)
                                ttCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTa                                                                                                                  <  2:1266620/150‑1 (MQ=255)
                                  cAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGACAACCCAAGTGGTGCTTTCGCCTGCACCATCCCCTGCGCTGCCATTACATCCGTAAt                                                                                                                >  1:981140/1‑150 (MQ=255)
                                      gTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGtt                                                                                                            <  2:205543/150‑1 (MQ=255)
                                       acGGGTTATCCAGTGCAATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTACTGAACACCAACTTTTGCCAGAAATAGGGCCAACCCAAGAGCTACTTACGACTGCACCATCCCCAGCGCGGCCATTACATCCGTAATAGTTa                                                                                                           >  2:286058/2‑150 (MQ=255)
                                          ggTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCCCCCTCCCCTGCGCTGCCATTACATCCGTAATTGTTAaac                                                                                                        >  2:1810066/1‑150 (MQ=255)
                                               tCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCg                                                                                                   <  2:1494681/150‑1 (MQ=255)
                                                ccAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGTCCAACCCAAGTGGTGCGTTCGACTGCAAAATCCCCTGCGCTGCCAATACATCCGTAATTGTTAAACAACCgg                                                                                                  >  1:286411/1‑150 (MQ=255)
                                                 cAGTGCCATCGAGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCCTCCCCTGCGCTGCCATTACATCCGGAATTGTTAAACACCCGGt                                                                                                 >  1:162683/1‑150 (MQ=255)
                                                    tGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGcc                                                                                              <  1:258216/150‑1 (MQ=255)
                                                     gCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGCGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCACCCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTACACAACCGGTGCCt                                                                                             >  1:1048813/1‑150 (MQ=255)
                                                     gCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGATAAACAACCGGTGCCt                                                                                             >  2:628146/1‑150 (MQ=255)
                                                      ccATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTg                                                                                            <  2:1721221/150‑1 (MQ=255)
                                                      ccATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTg                                                                                            >  2:845032/1‑150 (MQ=255)
                                                        aTCGCGTAATTTAGCAGGCCTTCAATAGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCGTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTc                                                                                          >  1:653024/1‑150 (MQ=255)
                                                         tCGCGTATTTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTTAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCg                                                                                         <  1:823920/150‑1 (MQ=255)
                                                          cgcgTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCAGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGCCTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGACGc                                                                                        >  2:693409/1‑150 (MQ=255)
                                                           gcgTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAACCAACCGGTGCCTGTCGCt                                                                                       >  2:1311444/1‑150 (MQ=255)
                                                              tAATTTAGCAGGCCTTCAATCGCAAACTGCGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGa                                                                                    >  1:1221250/1‑150 (MQ=255)
                                                               aaTTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAg                                                                                   >  1:32247/1‑150 (MQ=255)
                                                                 tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                 <  2:1654399/150‑1 (MQ=255)
                                                                 tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                 <  1:1131219/150‑1 (MQ=255)
                                                                 tttAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCg                                                                                 >  2:963446/1‑150 (MQ=255)
                                                                    aGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGTAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCCCCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGTCGCTGGAGCGTCa                                                                              >  2:877723/1‑150 (MQ=255)
                                                                        ggCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGc                                                                          >  2:1698155/1‑150 (MQ=255)
                                                                          ccTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACACCCGGTGCCTGTCGCTGGAGCGTCATCGCTc                                                                        >  2:561880/1‑150 (MQ=255)
                                                                          ccTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTc                                                                        >  2:618336/1‑150 (MQ=255)
                                                                               aaTCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAAttt                                                                   >  2:634946/1‑150 (MQ=255)
                                                                                aTCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAAtttt                                                                  >  2:1038432/1‑150 (MQ=255)
                                                                                 tcggtaaCTGAGGGTGCTTAACACCAACTTTTGCCAGAAATTAGGCCAACCGAAGTGGTGCTTTCGACTGCTCCATCCCCTGCGCTGCGATTAGATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGATCAATTTTg                                                                 <  2:1310161/145‑1 (MQ=255)
                                                                                   gCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGga                                                               <  1:1166951/150‑1 (MQ=255)
                                                                                    cAACCTGAGCGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGgag                                                              <  2:365842/150‑1 (MQ=255)
                                                                                      aaCTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGAt                                                            >  2:275341/1‑150 (MQ=255)
                                                                                            gggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGATTTCGACTGCACCATCCCCTGCGCTGCCATTAAATCCGTAAATGTTAAACAACCTGTGCCTGTCGCTGGAGCGTCAGCGATAAAGTTTGGAGATGTTggg                                                      >  2:1221455/1‑150 (MQ=255)
                                                                                             ggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTgggg                                                     <  1:1775323/150‑1 (MQ=255)
                                                                                             ggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTgggg                                                     <  2:979951/150‑1 (MQ=255)
                                                                                             ggTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTgggg                                                     >  2:1085183/1‑150 (MQ=255)
                                                                                               tCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAg                                                   <  1:1595247/150‑1 (MQ=255)
                                                                                                ccTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGt                                                  <  1:539715/150‑1 (MQ=255)
                                                                                                 cTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTa                                                 >  1:184170/1‑150 (MQ=255)
                                                                                                 cTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTa                                                 >  1:1676478/1‑150 (MQ=255)
                                                                                                  tGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTaa                                                >  2:1256453/1‑150 (MQ=255)
                                                                                                   taaCACCAACTTTTGCCAGAAATAAGGCCAACACAAGAGGTGCTTTCGACTGCACCATCACCTGCGCTGCCATTACCTCGGTAATTTTTAAACACACCGTGCCTGTCGCTGGAGCGTCATCGCTCAATTATCGAGATTTTGGGCAGTaaa                                               >  2:556050/2‑149 (MQ=255)
                                                                                                     acacCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGGTTTCGACTGCTCCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAatat                                             <  2:449193/150‑1 (MQ=255)
                                                                                                     acacCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATATTGGAGATTTTGGGGAGTAatat                                             >  2:535094/1‑150 (MQ=255)
                                                                                                         cAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTg                                         <  2:1819343/150‑1 (MQ=255)
                                                                                                          aaCTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                        <  2:1309881/150‑1 (MQ=255)
                                                                                                          aaCTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                        <  2:32247/150‑1 (MQ=255)
                                                                                                          aaCTGTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCGATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCGGGAGCATCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTgg                                        <  2:1649460/150‑1 (MQ=255)
                                                                                                            cTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTa                                      <  1:1038432/150‑1 (MQ=255)
                                                                                                                 gCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATATTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCt                                 >  2:544760/1‑150 (MQ=255)
                                                                                                                  ccAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCtt                                >  1:582596/1‑150 (MQ=255)
                                                                                                                   cAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTc                               >  2:1080104/1‑150 (MQ=255)
                                                                                                                    aGAACTACGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGGCATCGCTCAATTATGGAGATTTAGGGGAGTAATATTTTGGTAAGGCTTCa                              <  2:302048/150‑1 (MQ=255)
                                                                                                                    aGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAACCAACCGGTGCCTGTCGCTGGAGCGTCTTCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCa                              >  1:1603595/1‑150 (MQ=255)
                                                                                                                       aaTAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAgg                           <  1:217714/150‑1 (MQ=255)
                                                                                                                            tgCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCGGGCATTACATCCGTAATTGTTAAACAACCGGTTCCTGTCGCTGGAGAGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                      <  1:111622/149‑1 (MQ=255)
                                                                                                                            ggCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                      <  1:6404/150‑1 (MQ=255)
                                                                                                                            ggCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCAt                      >  2:1720894/1‑150 (MQ=255)
                                                                                                                              ccAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCTCTGCCATTACATCCGTAATTGTTAAACAACCGGTGAGTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTTATATTTTGGTAAGGCTTCAAGGTTCATGc                    <  1:5165/150‑1 (MQ=255)
                                                                                                                              ccAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGc                    >  2:477948/1‑150 (MQ=255)
                                                                                                                               cAACCCAAGTGGTGCTATCGACTGCACCATCCCCTGCGCTGCCATTACCTCCGTAATTGATAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGAATTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCt                   >  2:819947/1‑150 (MQ=255)
                                                                                                                                 aCCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCg                 <  1:1070165/150‑1 (MQ=255)
                                                                                                                                  cccAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTTCATCCGTAATTGTTAAACAACCGGTGCCTCTCGCTGGAGCGTAATCAATCAAATTTGTAGATTTTGTGTAGTAATATTTTGTTAAGGCTTCAAGGTTCATGCTAGt                >  2:1204565/1‑150 (MQ=255)
                                                                                                                                    cAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtc              <  1:1222785/150‑1 (MQ=255)
                                                                                                                                     ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtct             >  1:1258742/4‑150 (MQ=255)
                                                                                                                                     ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtct             >  2:818756/4‑150 (MQ=255)
                                                                                                                                     ggatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc            >  1:747233/4‑150 (MQ=255)
                                                                                                                                      gatggtgCAGTCGAAAGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc            >  2:395168/3‑150 (MQ=255)
                                                                                                                                      aGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTtctc            <  1:27350/150‑1 (MQ=255)
                                                                                                                                         ggtgCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTTATAGTTTGGGAAGGCGTCATGGTTCATGCTCGTTCTCCAc         >  1:274185/1‑150 (MQ=255)
                                                                                                                                          gtgCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACt        >  1:340053/1‑150 (MQ=255)
                                                                                                                                            gCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCGCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTa      <  1:555557/150‑1 (MQ=255)
                                                                                                                                               ttCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGc   >  2:1745429/1‑150 (MQ=255)
                                                                                                                                                tCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAACCAACCGGTGCCTGTCGCTGGAGCGGCGGCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGcc  <  1:1311444/150‑1 (MQ=255)
                                                                                                                                                tCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGcc  <  1:477948/150‑1 (MQ=255)
                                                                                                                                                tCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGCGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGcc  <  2:890099/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGCC  >  NC_000913/1642968‑1643261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: