Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,194 A→C 6.5% K709T (AAG→ACG)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,1940AC 6.5% 328.5 / 10.4 118K709T (AAG→ACG) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (57/53);  new base C (5/3);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 120 total aligned reads displayed.

AAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGC  >  NC_000913/1660046‑1660342
                                                                                                                                                    |                                                                                                                                                    
aaaGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAg                                                                                                                                                     <  1:1261295/150‑1 (MQ=255)
 aaGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAgg                                                                                                                                                    <  2:1413160/150‑1 (MQ=255)
         gTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcctgc                                                                                                                                            <  2:62306/150‑1 (MQ=255)
         gTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcctgc                                                                                                                                            >  1:1798033/1‑150 (MQ=255)
           gATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTTGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCg                                                                                                                                          <  1:1219778/150‑1 (MQ=255)
             tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATAGTGCACTCCTGCAGGCTGCCTGCCGTc                                                                                                                                        >  2:898505/1‑150 (MQ=255)
             tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTc                                                                                                                                        >  2:1145720/1‑150 (MQ=255)
             tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTc                                                                                                                                        <  1:1532860/150‑1 (MQ=255)
              aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCa                                                                                                                                       <  1:1323293/150‑1 (MQ=255)
               aGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCag                                                                                                                                      <  1:1425348/150‑1 (MQ=255)
                 cccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCTCTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagga                                                                                                                                    >  2:385323/1‑150 (MQ=255)
                  ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                   <  1:1787836/150‑1 (MQ=255)
                    aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGATCCTTCCCGCTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCTACCTTCGGCTATTTTGATATACTGAAGGCTGCCGGCCTGCAGGAGgt                                                                                                                                 >  1:71116/1‑150 (MQ=255)
                    aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggagat                                                                                                                                 <  2:1523994/150‑3 (MQ=255)
                    aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgt                                                                                                                                 >  1:1677131/1‑150 (MQ=255)
                     ttGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtg                                                                                                                                <  1:1515471/150‑1 (MQ=255)
                       gCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGtgtg                                                                                                                              <  2:1697924/150‑1 (MQ=255)
                       gCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGATGCCTGCCGTCAGGAGGtgtg                                                                                                                              >  2:611198/1‑150 (MQ=255)
                        ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACAGCAACTGTTTGGTTTCCATTACAAATCCCGTAGTCACTCGACCTACGGCAATATTGATCTCCTGAAGGATGCATGCCGTAAGGAGGTGTgg                                                                                                                             >  2:1471863/1‑150 (MQ=255)
                          cgTTTATGCCTCAACCTTTGCAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACTAATCCCGTACGCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                           <  1:362276/149‑1 (MQ=255)
                          aGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                           <  2:580732/150‑1 (MQ=255)
                           gTTTATGCCTCAACCTTTGAAGGCTGGGACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                          <  2:838297/150‑1 (MQ=255)
                           gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTTACTCGACCTACGGAAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                          <  2:615322/150‑1 (MQ=255)
                           gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                          <  2:1388407/150‑1 (MQ=255)
                             ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                        >  2:646068/1‑150 (MQ=255)
                             ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                        <  1:1790196/150‑1 (MQ=255)
                               aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAcc                                                                                                                      <  1:387368/150‑1 (MQ=255)
                                tGCCTTAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAATACCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAccc                                                                                                                     <  1:334618/150‑1 (MQ=255)
                                  ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                   >  2:1465422/1‑150 (MQ=255)
                                  ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                   <  1:118826/150‑1 (MQ=255)
                                  ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                   >  1:568078/1‑150 (MQ=255)
                                    tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGACCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                                 <  2:963552/150‑1 (MQ=255)
                                       aCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGAt                                                                                                              >  2:246852/1‑150 (MQ=255)
                                        ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCACCTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTTCGGCAATATTTATCTCCTGAAGGCTGCCTGCAGTCAGGAGGTGTGGATCAACCCTTTAGATg                                                                                                             >  2:1329068/1‑150 (MQ=255)
                                             gAAGGCTGGACCTACCATGAACGTAGAACCTTCCCACTGCAACTGGTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                        <  1:164527/150‑1 (MQ=255)
                                                ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCTATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                     <  1:1615358/150‑1 (MQ=255)
                                                ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGCTGCGCAGaaa                                                                                                     >  1:583118/1‑150 (MQ=255)
                                                ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATAACAAAGCCCGTACTCCCTCGACCTACGGCAATATAGATCTCCGGCAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                     >  2:118066/1‑150 (MQ=255)
                                                 gCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAAc                                                                                                    >  2:1133250/1‑150 (MQ=255)
                                                  cTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACg                                                                                                   >  1:686911/1‑150 (MQ=255)
                                                  cTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACg                                                                                                   >  2:1219354/1‑150 (MQ=255)
                                                       aCTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGa                                                                                              >  2:1056434/1‑150 (MQ=255)
                                                          ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                           >  1:1427906/1‑150 (MQ=255)
                                                           cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                          >  2:516263/1‑150 (MQ=255)
                                                            ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTTCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                         <  1:986581/150‑1 (MQ=255)
                                                             cTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcaa                                                                                        <  1:778544/150‑3 (MQ=255)
                                                               gAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAAc                                                                                      >  1:817645/1‑150 (MQ=255)
                                                                  cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                   <  1:519863/150‑1 (MQ=255)
                                                                   gTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCg                                                                                  <  2:694202/150‑1 (MQ=255)
                                                                         ccTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATgg                                                                            <  1:405129/150‑1 (MQ=255)
                                                                           ttCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAGCCCTATAGATGAGCAGAAACGTGGGATTGCCCACGGCGATATgggg                                                                          >  2:261556/1‑148 (MQ=255)
                                                                            tCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCAATCGACCTACGGAAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGc                                                                         <  2:371072/150‑1 (MQ=255)
                                                                             cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                        >  2:1295475/1‑150 (MQ=255)
                                                                                   gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                  <  2:68167/150‑1 (MQ=255)
                                                                                   gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCAATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                  >  2:1627202/1‑150 (MQ=255)
                                                                                   gCAACTGTTTGGCTTCCATTAGATCTCGCGTTCTCACTCGACCTCCGGCAATTTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCGCTATAGATTCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                  <  1:274676/150‑1 (MQ=255)
                                                                                              gTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTg                                                       <  2:1339715/150‑1 (MQ=255)
                                                                                                 tCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATGAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTTACCATCGTggag                                                    >  2:776632/1‑148 (MQ=255)
                                                                                                 tCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCg                                                    <  2:323832/150‑1 (MQ=255)
                                                                                                  ccATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGa                                                   <  2:20971/150‑1 (MQ=255)
                                                                                                    aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                 >  2:436322/1‑150 (MQ=255)
                                                                                                    aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                 >  1:527250/1‑150 (MQ=255)
                                                                                                        cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                             >  2:649328/1‑150 (MQ=255)
                                                                                                        cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATAAACCCTATAGATGCGCAGAAACGTCGGATTGCCAACGGCGATATCGTGCGGGTGTTTAACCATAGTGGCGTAGTTCg                                             >  1:147903/1‑150 (MQ=255)
                                                                                                                  actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATATATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                   <  2:1548010/150‑1 (MQ=255)
                                                                                                                  actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                   >  2:1078411/1‑150 (MQ=255)
                                                                                                                  actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                   >  1:801989/1‑150 (MQ=255)
                                                                                                                  actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                   >  2:650646/1‑150 (MQ=255)
                                                                                                                    tcactcGACCTACGGCAATCTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGACACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGGTCGGCTACCAGCCaa                                 <  1:1140240/150‑1 (MQ=255)
                                                                                                                     cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                                >  2:1263398/1‑150 (MQ=255)
                                                                                                                       ctcGACCTACGGCAATATTGATCTCCTGCAGGCTGCCTGCCGTCAGGAGGTGGGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGt                              <  2:1661072/150‑1 (MQ=255)
                                                                                                                          gACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                           <  1:804373/150‑1 (MQ=255)
                                                                                                                            ccTCCGGCAATATTGATCTCCTGAAGGCTGCCGGCCGTCAGGAGGTGTGGATCAACCCTAAAGATGCGCAGAAACGCGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                         <  1:132227/150‑1 (MQ=255)
                                                                                                                            ccTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                         >  1:540092/1‑150 (MQ=255)
                                                                                                                              tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                       <  1:1465422/150‑1 (MQ=255)
                                                                                                                                cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                     >  2:701507/1‑150 (MQ=255)
                                                                                                                                  gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                   <  1:742324/150‑1 (MQ=255)
                                                                                                                                  gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGGTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                   <  2:3470/150‑1 (MQ=255)
                                                                                                                                  gCAATATTGATCTCCTGAAGGCTGCCTGCCGACAGGAGGTGTGGATCCACCCTATAGATGCGAAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCAAAAGTAACACCACGTa                   >  1:395046/1‑150 (MQ=255)
                                                                                                                                         tGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAGCAACCCTATAGAAGCGCATAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTccc            <  1:898505/150‑1 (MQ=255)
                                                                                                                                         acacctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGAGGTATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACAACGTATtctcac            >  2:462775/5‑148 (MQ=255)
                                                                                                                                           aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg          <  2:580615/150‑1 (MQ=255)
                                                                                                                                           aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg          >  1:963913/1‑150 (MQ=255)
                                                                                                                                           aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg          >  2:117413/1‑150 (MQ=255)
                                                                                                                                            cctcCTGACGGCAGGCAGCCTTCAGGAGATGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg         >  1:485597/2‑150 (MQ=255)
                                                                                                                                              tcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg       >  2:910970/1‑150 (MQ=255)
                                                                                                                                              tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg       <  1:1750528/150‑1 (MQ=255)
                                                                                                                                              tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg       <  1:1133250/150‑1 (MQ=255)
                                                                                                                                              tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg       <  1:646068/150‑1 (MQ=255)
                                                                                                                                              tcCTGAAGGCTGCCTGCCGTCAGGAGGTGCGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg       <  1:1242708/150‑1 (MQ=255)
                                                                                                                                               ccTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACACTATAGATGAGAAGAAACGTGGAATTGCAAAAGTCGATATTGTGCGTATATTTAAAAATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt      >  2:280584/1‑150 (MQ=255)
                                                                                                                                               ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGTGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt      >  2:393929/1‑150 (MQ=255)
                                                                                                                                               ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt      >  1:154050/1‑150 (MQ=255)
                                                                                                                                               ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt      >  2:616863/1‑150 (MQ=255)
                                                                                                                                               ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt      >  2:1670512/1‑150 (MQ=255)
                                                                                                                                                cTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGtt     >  1:1124481/1‑150 (MQ=255)
                                                                                                                                                cTGAACGCTGCCTGACGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGATACGGGGGATTGCGAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCTAAGTAACACCACGTATTCTCCGTGGAGtt     <  2:1815306/150‑1 (MQ=255)
                                                                                                                                                 tGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTa    >  2:1151108/1‑150 (MQ=255)
                                                                                                                                                   aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc  <  1:516263/150‑1 (MQ=255)
                                                                                                                                                   aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc  <  1:1145720/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGC  >  NC_000913/1660046‑1660342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: