Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,198 T→A 6.6% A710A (GCT→GCA)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,1980TA 6.6% 323.5 / 9.3 116A710A (GCT→GCA) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (56/52);  new base A (5/3);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 121 total aligned reads displayed.

GTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAT  >  NC_000913/1660055‑1660347
                                                                                                                                               |                                                                                                                                                     
gTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcctgc                                                                                                                                                 >  1:1798033/1‑150 (MQ=255)
gTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcctgc                                                                                                                                                 <  2:62306/150‑1 (MQ=255)
 tGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCCACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCtgcctgcc                                                                                                                                                >  2:1208795/1‑150 (MQ=255)
  gATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTTGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCg                                                                                                                                               <  1:1219778/150‑1 (MQ=255)
    tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATAGTGCACTCCTGCAGGCTGCCTGCCGTc                                                                                                                                             >  2:898505/1‑150 (MQ=255)
    tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTc                                                                                                                                             >  2:1145720/1‑150 (MQ=255)
     aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCa                                                                                                                                            <  1:1323293/150‑1 (MQ=255)
        cccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCTCTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagga                                                                                                                                         >  2:385323/1‑150 (MQ=255)
         ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                        <  1:1787836/150‑1 (MQ=255)
           aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGATCCTTCCCGCTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCTACCTTCGGCTATTTTGATATACTGAAGGCTGCCGGCCTGCAGGAGgt                                                                                                                                      >  1:71116/1‑150 (MQ=255)
           aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggagat                                                                                                                                      <  2:1523994/150‑3 (MQ=255)
           aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgt                                                                                                                                      >  1:1677131/1‑150 (MQ=255)
            ttGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtg                                                                                                                                     <  1:1515471/150‑1 (MQ=255)
              gCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGtgtg                                                                                                                                   <  2:1697924/150‑1 (MQ=255)
                 cgTTTATGCCTCAACCTTTGCAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACTAATCCCGTACGCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                                <  1:362276/149‑1 (MQ=255)
                 aGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                                <  2:580732/150‑1 (MQ=255)
                  gTTTATGCCTCAACCTTTGAAGGCTGGGACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                               <  2:838297/150‑1 (MQ=255)
                  gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTTACTCGACCTACGGAAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                               <  2:615322/150‑1 (MQ=255)
                  gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                               <  2:1388407/150‑1 (MQ=255)
                    ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                             >  2:646068/1‑150 (MQ=255)
                    ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                             <  1:1790196/150‑1 (MQ=255)
                      aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAcc                                                                                                                           <  1:387368/150‑1 (MQ=255)
                       tGCCTTAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAATACCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAccc                                                                                                                          <  1:334618/150‑1 (MQ=255)
                        gCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCAATACAAATACCGTACTCACTCGACCTACGGCAATATAGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAccca                                                                                                                         >  2:646820/1‑149 (MQ=255)
                         ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                        <  1:118826/150‑1 (MQ=255)
                         ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                        >  2:1465422/1‑150 (MQ=255)
                         ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                        >  1:568078/1‑150 (MQ=255)
                           tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGACCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                                      <  2:963552/150‑1 (MQ=255)
                           tCAACCTTTGAAGGCTGGAACTCCCATGAACGTAGAACCTTCACACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAAGATTGATCTCATGAAGGCTGACTGCCTTCAGGAGGTGTGGATCAACCCtata                                                                                                                      >  1:1395437/1‑150 (MQ=255)
                                    gAAGGCTGGACCTACCATGAACGTAGAACCTTCCCACTGCAACTGGTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                             <  1:164527/150‑1 (MQ=255)
                                    gAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                             >  1:323832/1‑150 (MQ=255)
                                       ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCTATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                          <  1:1615358/150‑1 (MQ=255)
                                       ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGCTGCGCAGaaa                                                                                                          >  1:583118/1‑150 (MQ=255)
                                       ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATAACAAAGCCCGTACTCCCTCGACCTACGGCAATATAGATCTCCGGCAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                          >  2:118066/1‑150 (MQ=255)
                                        gCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAAc                                                                                                         >  2:1133250/1‑150 (MQ=255)
                                         cTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACg                                                                                                        >  2:1219354/1‑150 (MQ=255)
                                                tCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtt                                                                                                 >  1:1527102/1‑150 (MQ=255)
                                                 ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                >  1:1427906/1‑150 (MQ=255)
                                                 ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAAAATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtag                                                                                                >  2:1132593/1‑148 (MQ=255)
                                                  cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                               >  2:516263/1‑150 (MQ=255)
                                                   ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTTCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                              <  1:986581/150‑1 (MQ=255)
                                                    cTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcaa                                                                                             <  1:778544/150‑3 (MQ=255)
                                                      gAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAAc                                                                                           >  1:817645/1‑150 (MQ=255)
                                                         cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                        <  1:519863/150‑1 (MQ=255)
                                                          gTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCg                                                                                       <  2:694202/150‑1 (MQ=255)
                                                             gAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                                    >  2:742324/1‑150 (MQ=255)
                                                                ccTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATgg                                                                                 <  1:405129/150‑1 (MQ=255)
                                                                  ttCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAGCCCTATAGATGAGCAGAAACGTGGGATTGCCCACGGCGATATgggg                                                                               >  2:261556/1‑148 (MQ=255)
                                                                    cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                             >  2:1295475/1‑150 (MQ=255)
                                                                          gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                       >  1:467511/1‑150 (MQ=255)
                                                                          gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                       <  2:68167/150‑1 (MQ=255)
                                                                          gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCAATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                       >  2:1627202/1‑150 (MQ=255)
                                                                          gCAACTGTTTGGCTTCCATTAGATCTCGCGTTCTCACTCGACCTCCGGCAATTTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCGCTATAGATTCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                       <  1:274676/150‑1 (MQ=255)
                                                                                     gTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTg                                                            <  2:1339715/150‑1 (MQ=255)
                                                                                       ttCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGGCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGc                                                          <  2:608960/150‑1 (MQ=255)
                                                                                        tCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCg                                                         <  2:323832/150‑1 (MQ=255)
                                                                                         ccATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGa                                                        <  2:20971/150‑1 (MQ=255)
                                                                                          cATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGaa                                                       <  1:729125/150‑1 (MQ=255)
                                                                                               cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                                  >  2:401147/1‑150 (MQ=255)
                                                                                               cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATAAACCCTATAGATGCGCAGAAACGTCGGATTGCCAACGGCGATATCGTGCGGGTGTTTAACCATAGTGGCGTAGTTCg                                                  >  1:147903/1‑150 (MQ=255)
                                                                                               cAAATCCCGTACTCACTCGACCTACGGCAAAATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATAGTGGCGAAGTTCg                                                  >  2:323178/1‑150 (MQ=255)
                                                                                                aaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCgg                                                 >  2:1350450/1‑150 (MQ=255)
                                                                                                aaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCgg                                                 <  2:1814541/150‑1 (MQ=255)
                                                                                                    cccGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTAc                                             >  1:555055/1‑150 (MQ=255)
                                                                                                         actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATATATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                        <  2:1548010/150‑1 (MQ=255)
                                                                                                         actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                        >  2:1078411/1‑150 (MQ=255)
                                                                                                         actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                        >  2:650646/1‑150 (MQ=255)
                                                                                                           tcactcGACCTACGGCAATCTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGACACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGGTCGGCTACCAGCCaa                                      <  1:1140240/150‑1 (MQ=255)
                                                                                                            cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                                     >  2:1263398/1‑150 (MQ=255)
                                                                                                              ctcGACCTACGGCAATATTGATCTCCTGCAGGCTGCCTGCCGTCAGGAGGTGGGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGt                                   <  2:1661072/150‑1 (MQ=255)
                                                                                                                 gACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                <  1:804373/150‑1 (MQ=255)
                                                                                                                 gACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                <  2:1527102/150‑1 (MQ=255)
                                                                                                                   ccTCCGGCAATATTGATCTCCTGAAGGCTGCCGGCCGTCAGGAGGTGTGGATCAACCCTAAAGATGCGCAGAAACGCGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                              <  1:132227/150‑1 (MQ=255)
                                                                                                                   ccTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                              >  1:540092/1‑150 (MQ=255)
                                                                                                                     tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                            <  1:1465422/150‑1 (MQ=255)
                                                                                                                       cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                          >  2:701507/1‑150 (MQ=255)
                                                                                                                         gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                        <  1:742324/150‑1 (MQ=255)
                                                                                                                         gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGGTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                        <  2:3470/150‑1 (MQ=255)
                                                                                                                                tGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAGCAACCCTATAGAAGCGCATAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTccc                 <  1:898505/150‑1 (MQ=255)
                                                                                                                                acacctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGAGGTATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACAACGTATtctcac                 >  2:462775/5‑148 (MQ=255)
                                                                                                                                  aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg               >  1:963913/1‑150 (MQ=255)
                                                                                                                                  aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg               <  2:580615/150‑1 (MQ=255)
                                                                                                                                   cctcCTGACGGCAGGCAGCCTTCAGGAGATGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg              >  1:485597/2‑150 (MQ=255)
                                                                                                                                     tcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg            >  2:910970/1‑150 (MQ=255)
                                                                                                                                     tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg            <  1:1750528/150‑1 (MQ=255)
                                                                                                                                     tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg            <  1:1133250/150‑1 (MQ=255)
                                                                                                                                     tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg            <  1:646068/150‑1 (MQ=255)
                                                                                                                                     tcCTGAAGGCTGCCTGCCGTCAGGAGGTGCGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg            <  1:1242708/150‑1 (MQ=255)
                                                                                                                                      ccTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACACTATAGATGAGAAGAAACGTGGAATTGCAAAAGTCGATATTGTGCGTATATTTAAAAATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt           >  2:280584/1‑150 (MQ=255)
                                                                                                                                      ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGTGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt           >  2:393929/1‑150 (MQ=255)
                                                                                                                                      ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt           >  1:154050/1‑150 (MQ=255)
                                                                                                                                      ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt           >  2:616863/1‑150 (MQ=255)
                                                                                                                                       cTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGtt          >  1:1124481/1‑150 (MQ=255)
                                                                                                                                       cTGAACGCTGCCTGACGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGATACGGGGGATTGCGAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCTAAGTAACACCACGTATTCTCCGTGGAGtt          <  2:1815306/150‑1 (MQ=255)
                                                                                                                                        tGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTa         >  2:1151108/1‑150 (MQ=255)
                                                                                                                                          atggCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTTTAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTGGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc       <  2:408435/148‑1 (MQ=255)
                                                                                                                                          aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc       <  1:1145720/150‑1 (MQ=255)
                                                                                                                                          aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc       <  1:516263/150‑1 (MQ=255)
                                                                                                                                               tgcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAt  >  2:226902/1‑150 (MQ=255)
                                                                                                                                               tgcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACAAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAt  >  1:1573536/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAT  >  NC_000913/1660055‑1660347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑