Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,206 G→T 8.1% R713L (CGT→CTT)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,2060GT 8.1% 347.4 / 12.4 120R713L (CGT→CTT) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (59/51);  new base T (7/3);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGG  >  NC_000913/1660057‑1660350
                                                                                                                                                     |                                                                                                                                                
gATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTTGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCg                                                                                                                                                  <  1:1219778/150‑1 (MQ=255)
  tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATAGTGCACTCCTGCAGGCTGCCTGCCGTc                                                                                                                                                >  2:898505/1‑150 (MQ=255)
  tAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTc                                                                                                                                                >  2:1145720/1‑150 (MQ=255)
   aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCa                                                                                                                                               <  1:1323293/150‑1 (MQ=255)
       ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                           <  1:1787836/150‑1 (MQ=255)
         aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGATCCTTCCCGCTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCTACCTTCGGCTATTTTGATATACTGAAGGCTGCCGGCCTGCAGGAGgt                                                                                                                                         >  1:71116/1‑150 (MQ=255)
         aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgt                                                                                                                                         >  1:1677131/1‑150 (MQ=255)
          ttGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtg                                                                                                                                        <  1:1515471/150‑1 (MQ=255)
            gCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGATGCCTGCCGTCAGGAGGtgtg                                                                                                                                      >  2:611198/1‑150 (MQ=255)
             ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACAGCAACTGTTTGGTTTCCATTACAAATCCCGTAGTCACTCGACCTACGGCAATATTGATCTCCTGAAGGATGCATGCCGTAAGGAGGTGTgg                                                                                                                                     >  2:1471863/1‑150 (MQ=255)
               cgTTTATGCCTCAACCTTTGCAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACTAATCCCGTACGCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                                   <  1:362276/149‑1 (MQ=255)
               aGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt                                                                                                                                   <  2:580732/150‑1 (MQ=255)
                gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc                                                                                                                                  <  2:1388407/150‑1 (MQ=255)
                  ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                                >  2:646068/1‑150 (MQ=255)
                  ttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCaa                                                                                                                                <  1:1790196/150‑1 (MQ=255)
                    aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAcc                                                                                                                              <  1:387368/150‑1 (MQ=255)
                     tGCCTTAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAATACCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAccc                                                                                                                             <  1:334618/150‑1 (MQ=255)
                      gCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCAATACAAATACCGTACTCACTCGACCTACGGCAATATAGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAccca                                                                                                                            >  2:646820/1‑149 (MQ=255)
                       ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                           >  1:568078/1‑150 (MQ=255)
                       ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                           >  2:1465422/1‑150 (MQ=255)
                       ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                           <  1:118826/150‑1 (MQ=255)
                         tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGACCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                                         <  2:963552/150‑1 (MQ=255)
                         tCAACCTTTGAAGGCTGGAACTCCCATGAACGTAGAACCTTCACACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAAGATTGATCTCATGAAGGCTGACTGCCTTCAGGAGGTGTGGATCAACCCtata                                                                                                                         >  1:1395437/1‑150 (MQ=255)
                            aCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGAt                                                                                                                      >  2:246852/1‑150 (MQ=255)
                             ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCACCTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTTCGGCAATATTTATCTCCTGAAGGCTGCCTGCAGTCAGGAGGTGTGGATCAACCCTTTAGATg                                                                                                                     >  2:1329068/1‑150 (MQ=255)
                                  gAAGGCTGGACCTACCATGAACGTAGAACCTTCCCACTGCAACTGGTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                                <  1:164527/150‑1 (MQ=255)
                                  gAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                                >  1:323832/1‑150 (MQ=255)
                                     ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCTATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                             <  1:1615358/150‑1 (MQ=255)
                                     ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGCTGCGCAGaaa                                                                                                             >  1:583118/1‑150 (MQ=255)
                                     ggCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATAACAAAGCCCGTACTCCCTCGACCTACGGCAATATAGATCTCCGGCAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGaaa                                                                                                             >  2:118066/1‑150 (MQ=255)
                                      gCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAAc                                                                                                            >  2:1133250/1‑150 (MQ=255)
                                       cTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACg                                                                                                           >  2:1219354/1‑150 (MQ=255)
                                              tCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtt                                                                                                    >  1:1527102/1‑150 (MQ=255)
                                               ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGCCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                   >  1:1427906/1‑150 (MQ=255)
                                               ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAAAATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtag                                                                                                   >  2:1132593/1‑148 (MQ=255)
                                                cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                                  >  2:516263/1‑150 (MQ=255)
                                                 ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTTCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                                 <  1:986581/150‑1 (MQ=255)
                                                  cTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcaa                                                                                                <  1:778544/150‑3 (MQ=255)
                                                    gAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAAc                                                                                              >  1:817645/1‑150 (MQ=255)
                                                       cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                           <  1:519863/150‑1 (MQ=255)
                                                        gTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCg                                                                                          <  2:694202/150‑1 (MQ=255)
                                                           gAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                                       >  2:742324/1‑150 (MQ=255)
                                                              ccTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATgg                                                                                    <  1:405129/150‑1 (MQ=255)
                                                                 tCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCAATCGACCTACGGAAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGc                                                                                 <  2:371072/150‑1 (MQ=255)
                                                                  cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                                >  2:1295475/1‑150 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                          >  1:467511/1‑150 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                          <  2:68167/150‑1 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCAATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                          >  2:1627202/1‑150 (MQ=255)
                                                                        gCAACTGTTTGGCTTCCATTAGATCTCGCGTTCTCACTCGACCTCCGGCAATTTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCGCTATAGATTCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                          <  1:274676/150‑1 (MQ=255)
                                                                                     ttCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGGCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGc                                                             <  2:608960/150‑1 (MQ=255)
                                                                                      tCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATGAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTTACCATCGTggag                                                            >  2:776632/1‑148 (MQ=255)
                                                                                      tCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCg                                                            <  2:323832/150‑1 (MQ=255)
                                                                                       ccATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGa                                                           <  2:20971/150‑1 (MQ=255)
                                                                                        cATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGaa                                                          <  1:729125/150‑1 (MQ=255)
                                                                                         aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                         >  2:436322/1‑150 (MQ=255)
                                                                                             cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                                     >  2:401147/1‑150 (MQ=255)
                                                                                             cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                                     >  2:649328/1‑150 (MQ=255)
                                                                                             cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATAAACCCTATAGATGCGCAGAAACGTCGGATTGCCAACGGCGATATCGTGCGGGTGTTTAACCATAGTGGCGTAGTTCg                                                     >  1:147903/1‑150 (MQ=255)
                                                                                             cAAATCCCGTACTCACTCGACCTACGGCAAAATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATAGTGGCGAAGTTCg                                                     >  2:323178/1‑150 (MQ=255)
                                                                                              aaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCgg                                                    <  2:1814541/150‑1 (MQ=255)
                                                                                              aaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCgg                                                    >  2:1350450/1‑150 (MQ=255)
                                                                                                  cccGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTAc                                                >  1:555055/1‑150 (MQ=255)
                                                                                                       actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATATATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                           <  2:1548010/150‑1 (MQ=255)
                                                                                                       actcactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                           >  2:1078411/1‑150 (MQ=255)
                                                                                                         tcactcGACCTACGGCAATCTTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGACACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGGTCGGCTACCAGCCaa                                         <  1:1140240/150‑1 (MQ=255)
                                                                                                          cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                                        >  2:1263398/1‑150 (MQ=255)
                                                                                                            ctcGACCTACGGCAATATTGATCTCCTGCAGGCTGCCTGCCGTCAGGAGGTGGGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGt                                      <  2:1661072/150‑1 (MQ=255)
                                                                                                               gACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                   <  2:1527102/150‑1 (MQ=255)
                                                                                                               gACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                   <  1:804373/150‑1 (MQ=255)
                                                                                                                 ccTCCGGCAATATTGATCTCCTGAAGGCTGCCGGCCGTCAGGAGGTGTGGATCAACCCTAAAGATGCGCAGAAACGCGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                                 <  1:132227/150‑1 (MQ=255)
                                                                                                                 ccTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAacac                                 >  1:540092/1‑150 (MQ=255)
                                                                                                                   tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                               <  1:1465422/150‑1 (MQ=255)
                                                                                                                     cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                             >  2:701507/1‑150 (MQ=255)
                                                                                                                       gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                           <  1:742324/150‑1 (MQ=255)
                                                                                                                       gCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGGTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTa                           <  2:3470/150‑1 (MQ=255)
                                                                                                                              tGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAGCAACCCTATAGAAGCGCATAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTccc                    <  1:898505/150‑1 (MQ=255)
                                                                                                                                aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                  <  2:580615/150‑1 (MQ=255)
                                                                                                                                aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                  >  1:963913/1‑150 (MQ=255)
                                                                                                                                 cctcCTGACGGCAGGCAGCCTTCAGGAGATGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg                 >  1:485597/2‑150 (MQ=255)
                                                                                                                                   tcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg               >  2:910970/1‑150 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg               <  1:1750528/150‑1 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg               <  1:1133250/150‑1 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg               <  1:646068/150‑1 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGCGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg               <  1:1242708/150‑1 (MQ=255)
                                                                                                                                    ccTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACACTATAGATGAGAAGAAACGTGGAATTGCAAAAGTCGATATTGTGCGTATATTTAAAAATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt              >  2:280584/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGTGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt              >  2:393929/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt              >  1:154050/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt              >  2:1670512/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt              >  2:616863/1‑150 (MQ=255)
                                                                                                                                     cTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGtt             >  1:1124481/1‑150 (MQ=255)
                                                                                                                                     cTGAACGCTGCCTGACGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGATACGGGGGATTGCGAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCTAAGTAACACCACGTATTCTCCGTGGAGtt             <  2:1815306/150‑1 (MQ=255)
                                                                                                                                      tGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTa            >  2:1151108/1‑150 (MQ=255)
                                                                                                                                        atggCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTTTAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTGGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc          <  2:408435/148‑1 (MQ=255)
                                                                                                                                        aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc          <  1:1145720/150‑1 (MQ=255)
                                                                                                                                        aaGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAgc          <  1:516263/150‑1 (MQ=255)
                                                                                                                                          ggCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTCTTCTCCCTGGAGTTAgcgc        >  1:260360/1‑150 (MQ=255)
                                                                                                                                             tgcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAt     >  2:226902/1‑150 (MQ=255)
                                                                                                                                             tgcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACAAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAt     >  1:1573536/1‑150 (MQ=255)
                                                                                                                                               cctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATgg   >  2:618132/1‑150 (MQ=255)
                                                                                                                                                ctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATggg  >  2:863443/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGG  >  NC_000913/1660057‑1660350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: