Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,666,470 C→A 5.1% L386I (CTA→ATA)  clcB → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,4700CA 5.1% 362.4 / 7.3 118L386I (CTA→ATA) clcBH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (52/60);  new base A (4/2);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.17e-01

Only 100 of 121 total aligned reads displayed.

ATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAGG  >  NC_000913/1666321‑1666619
                                                                                                                                                     |                                                                                                                                                     
aTGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGACGATTATGTCGACGTTGATGATATGTGAAATGACCGGGTAGTATCAGc                                                                                                                                                       <  2:812986/150‑1 (MQ=255)
aTGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTAACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCAAGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGc                                                                                                                                                       <  1:1569522/150‑1 (MQ=255)
 tGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGCCCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCt                                                                                                                                                      <  2:1575600/150‑1 (MQ=255)
  ggTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAATTAACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCTGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTa                                                                                                                                                     >  1:40604/1‑150 (MQ=255)
         gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg                                                                                                                                              <  1:600554/150‑1 (MQ=255)
         cccTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCg                                                                                                                                              <  1:763368/149‑1 (MQ=255)
          ccTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCTACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                                                                             <  1:1291164/150‑1 (MQ=255)
          ccTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                                                                             <  1:615873/150‑1 (MQ=255)
           cTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                                                                            >  1:569616/1‑150 (MQ=255)
            tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                                                                           >  1:1642012/1‑150 (MQ=255)
             gggATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGttt                                                                                                                                          <  2:1033309/150‑1 (MQ=255)
                 ttATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattg                                                                                                                                      <  2:1309638/150‑1 (MQ=255)
                 ttATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattg                                                                                                                                      <  2:178951/150‑1 (MQ=255)
                 ataaggTACCCTGATGGCGAAGAAATTACACAATTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattg                                                                                                                                      <  2:456724/146‑1 (MQ=255)
                  tATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattga                                                                                                                                     <  2:1733499/150‑1 (MQ=255)
                  tATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattga                                                                                                                                     >  1:1679433/1‑150 (MQ=255)
                   aTGGTTCCCTCATGGCGACGAAATTACACTTTAACTCGGATTGAGCGGGATGGCGACACTGTTGGGGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgat                                                                                                                                    <  1:1640187/150‑1 (MQ=255)
                    tGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgatt                                                                                                                                   <  1:1756770/150‑1 (MQ=255)
                      gTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGc                                                                                                                                 <  2:1344986/150‑1 (MQ=255)
                       ttCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGCCCGGGAGGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGcc                                                                                                                                <  1:1418854/150‑1 (MQ=255)
                         cccTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTg                                                                                                                              <  1:1680092/150‑1 (MQ=255)
                           cTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCCGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                                                                            >  1:1193992/1‑150 (MQ=255)
                           cTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                                                                            <  2:13808/150‑1 (MQ=255)
                            tGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTTGTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGGGACATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGt                                                                                                                           <  1:76117/150‑1 (MQ=255)
                                 gCGAAGAATTTACACTTTTACTCGGATAGACCAGGATGGCGTCACTGTTGGCGGCGACCACGCACGCGCCGATTATGTAGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCAGGTTTATTGATTGCCTGCGTAATTg                                                                                                                      <  2:250174/150‑1 (MQ=255)
                                  cGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGc                                                                                                                     <  1:1530326/150‑1 (MQ=255)
                                  cGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGc                                                                                                                     >  1:1764359/1‑150 (MQ=255)
                                     agaaATTAAAATTTTACTAGTATTGACCTGGATGGAGAAAATGTTTGCTTCGAACACTAAATCTCCTATTATTTATACGTTTATTATATGTTAAATGACCTGTGAGTATCATCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCTTc                                                                                                                  <  1:1281847/150‑1 (MQ=255)
                                       aaaTTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCgg                                                                                                                <  1:424723/150‑1 (MQ=255)
                                           tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTTCGTAATTGCGTCGGTAAt                                                                                                            <  1:1581461/150‑1 (MQ=255)
                                            acacTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAtt                                                                                                           >  2:953274/1‑150 (MQ=255)
                                             cacTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAttt                                                                                                          >  1:1430640/1‑150 (MQ=255)
                                             cacTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTAGTGATTGCCTGCGTAAGTGCGTCGGTAAttt                                                                                                          >  2:275123/1‑150 (MQ=255)
                                               cTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                        >  1:1037889/1‑150 (MQ=255)
                                               cTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                        >  1:1074834/1‑150 (MQ=255)
                                               cTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                        >  1:1033179/1‑150 (MQ=255)
                                               cTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                        >  2:1650267/1‑150 (MQ=255)
                                                ttttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                                                                       <  1:100182/150‑1 (MQ=255)
                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                                                                      <  1:1008106/150‑1 (MQ=255)
                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                                                                      <  1:1455877/150‑1 (MQ=255)
                                                     cTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGAGATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATAGCGTCGGTAATTTCGCGGACg                                                                                                  >  2:168865/1‑150 (MQ=255)
                                                        ggATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTa                                                                                               <  2:1732625/150‑1 (MQ=255)
                                                         gATTGTCCGTGATGGCGTCACTGTTGGCGGGGACGACGCACGCGCCGAATATGTCGACGTTGATGATATGTGAAATGACGGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTac                                                                                              <  2:1013663/150‑1 (MQ=255)
                                                         gATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTac                                                                                              <  2:815059/150‑1 (MQ=255)
                                                             gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCg                                                                                          >  1:1682016/1‑150 (MQ=255)
                                                               ccGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTg                                                                                        <  1:953274/150‑1 (MQ=255)
                                                                     tGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtcta                                                                                  <  1:826661/150‑1 (MQ=255)
                                                                     tGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGACTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtcta                                                                                  >  1:1623461/1‑150 (MQ=255)
                                                                      ggCGACACTGTTGGCGGCGACCACGCACGCGCCGTTTATGTCGACGTTGATGATATGTGAAATGCCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGCGACtctat                                                                                 <  1:148687/150‑1 (MQ=255)
                                                                        cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGTTTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                               >  2:645484/1‑150 (MQ=255)
                                                                        cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                               >  1:208892/1‑150 (MQ=255)
                                                                        cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                               >  2:916451/1‑150 (MQ=255)
                                                                         gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatcta                                                                              <  1:1196154/150‑1 (MQ=255)
                                                                           cacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAcc                                                                            <  1:1219595/150‑1 (MQ=255)
                                                                           cacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTACCCGGTTGATTGCTTGCCTGCGTAATTGCGTCGGGAATTTCGCGGCCGTTACACCGTGACTCTATCTAcc                                                                            >  1:913079/1‑150 (MQ=255)
                                                                             cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATTATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGCCTCGATCTAACGc                                                                          >  1:861186/1‑150 (MQ=255)
                                                                                tttgcggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAg                                                                       <  1:606960/147‑1 (MQ=255)
                                                                                     ggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac                                                                  >  1:504899/1‑150 (MQ=255)
                                                                                         aCCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcg                                                              <  2:1642012/150‑1 (MQ=255)
                                                                                          ccACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGTGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc                                                             <  1:316291/150‑1 (MQ=255)
                                                                                          ccACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc                                                             >  2:854693/1‑150 (MQ=255)
                                                                                          ccACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGGTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc                                                             >  1:809784/1‑150 (MQ=255)
                                                                                          ccACGCACGCGCCAATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc                                                             >  1:813374/1‑150 (MQ=255)
                                                                                           cacgcacgCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgca                                                            >  1:613194/1‑150 (MQ=255)
                                                                                             cgcacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagc                                                          <  2:433285/150‑1 (MQ=255)
                                                                                                acgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATa                                                       <  2:1430640/150‑1 (MQ=255)
                                                                                                   cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTTCTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCt                                                    <  1:676754/150‑1 (MQ=255)
                                                                                                            tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCAATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATg                                           >  1:314675/1‑150 (MQ=255)
                                                                                                             gTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCTCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGt                                          >  1:550336/1‑150 (MQ=255)
                                                                                                             gTCGACGTTGATGATAAGTGAAATGACCGCGGAGTATCATCTACTCCCCGGTTTATTGATTGCCTGCTTCATTGCGACCGAAATATCGAGTAAGTTACACCGTAACTAAATCTTCCGCCATCTCACTGCGCAGCATATCTAAGCGTATGt                                          >  2:1102018/1‑150 (MQ=255)
                                                                                                              tCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACCCCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGAGGATGtt                                         >  2:970031/1‑149 (MQ=255)
                                                                                                              gcGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTa                                         <  2:1567359/149‑1 (MQ=255)
                                                                                                               cGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTAt                                        >  1:1345350/1‑150 (MQ=255)
                                                                                                                 aCGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTg                                      <  2:936463/150‑1 (MQ=255)
                                                                                                                  cGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGc                                     <  1:446462/150‑1 (MQ=255)
                                                                                                                  cGTTGATGATATGTGAAATGACCGGCGAGTTTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACTCCGTGACTCTATCTACCGCCAGCACACTGCGCAGCAGAGCTAAGCGGATGTATTGc                                     >  1:1100753/1‑150 (MQ=255)
                                                                                                                  cGTTGATGATATGTGAAAGGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGc                                     <  2:237308/150‑1 (MQ=255)
                                                                                                                       atgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAg                                <  1:843364/150‑1 (MQ=255)
                                                                                                                          atttgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTc                             <  1:1650156/147‑1 (MQ=255)
                                                                                                                           tatGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCa                            >  1:1643750/1‑150 (MQ=255)
                                                                                                                            ttgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc                           <  1:1423051/149‑1 (MQ=255)
                                                                                                                            atGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCTGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc                           <  1:1270477/150‑1 (MQ=255)
                                                                                                                             tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg                          <  2:813374/150‑1 (MQ=255)
                                                                                                                              ttgAAATGACCGGGGAGTATCAGCTACTCCCCAGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGc                         <  2:1792264/149‑1 (MQ=255)
                                                                                                                              gtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTTGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGc                         >  1:562314/1‑150 (MQ=255)
                                                                                                                              gtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGc                         <  1:561978/150‑1 (MQ=255)
                                                                                                                                    tGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCg                   <  1:202211/150‑1 (MQ=255)
                                                                                                                                     aaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTATGCGGATGTATTGCCCCAGTTCACGCTGTTCgg                  >  1:921724/2‑150 (MQ=255)
                                                                                                                                      aCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgc                 >  1:552521/1‑150 (MQ=255)
                                                                                                                                      aCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgc                 >  2:1455840/1‑150 (MQ=255)
                                                                                                                                       ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcg                >  1:1342382/1‑150 (MQ=255)
                                                                                                                                       ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcg                <  2:1074834/150‑1 (MQ=255)
                                                                                                                                         ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcgcg              >  1:1492492/1‑150 (MQ=255)
                                                                                                                                         ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcgcg              >  2:661186/1‑150 (MQ=255)
                                                                                                                                         ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCAAAGCGGTTGTATTGCCCCAGTTCACGCTGTTCGgcgcg              >  2:40973/1‑150 (MQ=255)
                                                                                                                                              gTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCa         >  2:1711290/1‑150 (MQ=255)
                                                                                                                                              gTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCa         <  1:1374990/150‑1 (MQ=255)
                                                                                                                                                aTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAGTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGa       >  2:1560807/1‑150 (MQ=255)
                                                                                                                                                  cAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATa     >  2:934436/1‑150 (MQ=255)
                                                                                                                                                     cTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAgg  >  1:235857/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAGG  >  NC_000913/1666321‑1666619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: