Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,679,644 A→T 5.3% M30L (ATG→TTG)  ydgI → putative arginine/ornithine antiporter transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,6440AT 5.3% 299.5 / 4.5 104M30L (ATG→TTG) ydgIputative arginine/ornithine antiporter transporter
Reads supporting (aligned to +/- strand):  ref base A (53/45);  new base T (4/2);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 105 total aligned reads displayed.

ATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGG  >  NC_000913/1679496‑1679789
                                                                                                                                                    |                                                                                                                                                 
aTTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAatat                                                                                                                                                  >  1:1116456/1‑150 (MQ=255)
   tttATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATggc                                                                                                                                               <  2:1069843/150‑1 (MQ=255)
    ttATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATggcg                                                                                                                                              >  1:1072387/1‑150 (MQ=255)
      aTGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATggcggc                                                                                                                                            <  2:1473032/150‑1 (MQ=255)
      aTGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATggcggc                                                                                                                                            <  2:408222/150‑1 (MQ=255)
       tGCGTTTTGCCTTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCa                                                                                                                                           >  2:1597509/1‑150 (MQ=255)
          gTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCCATATTTTGCGGCAgac                                                                                                                                        <  2:446668/150‑3 (MQ=255)
           ttttGCCTTAATAATTATTCTACTTATCAAGATTTAATCTCTGCATATCCATGGAATAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGATTTCAGTCTGCCGCAAAATATGGCGGCAGTTg                                                                                                                                       <  2:326318/150‑1 (MQ=255)
           ttttGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTg                                                                                                                                       >  2:1430819/1‑150 (MQ=255)
            tttGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGc                                                                                                                                      <  1:1803240/150‑1 (MQ=255)
                cccTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGc                                                                                                                                  >  1:1747897/1‑150 (MQ=255)
                      tatttattCTTCTTATCACGTTTTAATCACTGGATATCGATGGAACAGAAACTGTGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCGGCAAAATATGGCGGCAGTTGCCAGCCCGgca                                                                                                                            <  2:1468685/150‑1 (MQ=255)
                       atttattCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAACCTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGgcag                                                                                                                           <  2:1140779/150‑1 (MQ=255)
                       atttattCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGAGGCAGTTGCCAGCCCGgcag                                                                                                                           >  2:199817/1‑150 (MQ=255)
                              cttcttATCACGTTTTAATCACTGGATATCGATGGAAAAGAAGCTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCt                                                                                                                    >  2:1348475/1‑150 (MQ=255)
                              cttcttATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCt                                                                                                                    >  1:173791/1‑150 (MQ=255)
                              cttcttATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCt                                                                                                                    >  2:1732341/1‑150 (MQ=255)
                              cttcttATCACGTTATAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCt                                                                                                                    <  2:126459/150‑1 (MQ=255)
                               tacatATCACGTTATAATCACTGGATATCGATGGAAAAGAAACTGGGAATGAGCGCACTCACCGCGCAGTTATTAACCTCAATGATGGGCGCGGGTGTTTACAGTCTCCCGCAAAAAATGGCGGCAGTTGACAGCCCGGCAGCACTGCTc                                                                                                                   >  2:812988/5‑150 (MQ=255)
                                   tATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAACACTGCTCATCg                                                                                                               >  2:1823153/1‑150 (MQ=255)
                                     tCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCggg                                                                                                             >  1:708113/1‑149 (MQ=255)
                                      cACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCt                                                                                                            >  1:403485/1‑150 (MQ=255)
                                        cGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTgg                                                                                                          <  1:546595/150‑1 (MQ=255)
                                         gTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTggg                                                                                                         <  2:1560662/150‑1 (MQ=255)
                                          ttttAATCACTGGATATCTATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTgggg                                                                                                        <  1:764799/150‑1 (MQ=255)
                                          ttttAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTgggg                                                                                                        <  1:522186/150‑1 (MQ=255)
                                           tttAATCACTGGATATCGATGGAAAAGATACTGGGACTGAGCGGACTCACCGCGCTGGGATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGt                                                                                                       <  1:1135612/150‑1 (MQ=255)
                                           tttAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGt                                                                                                       <  2:1332745/150‑1 (MQ=255)
                                                  aCTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTg                                                                                                >  2:1696409/1‑150 (MQ=255)
                                                   cTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTgg                                                                                               >  1:1595721/1‑150 (MQ=255)
                                                       atatCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCt                                                                                           <  2:418985/150‑1 (MQ=255)
                                                       atatCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCt                                                                                           <  1:1012276/150‑1 (MQ=255)
                                                        tatCTATGTAAAAGAAACTGGGACTTAGCGCACTAAGCGCGCTGTTATTAATCTCACTGCTGGGCTCGCGTGTATTCAGTCTGCCGCAAAAAATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGTCGCTg                                                                                          <  2:336163/150‑1 (MQ=255)
                                                        tatCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTg                                                                                          <  1:1234487/150‑1 (MQ=255)
                                                          tCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGc                                                                                        >  1:271792/1‑150 (MQ=255)
                                                           cGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCa                                                                                       >  1:369851/1‑150 (MQ=255)
                                                                gAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGTCATTTTa                                                                                  >  2:211149/1‑150 (MQ=255)
                                                                 aaaaGACACTGGGATTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTAt                                                                                 <  2:645219/150‑1 (MQ=255)
                                                                    aGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATTGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGc                                                                              >  2:655175/1‑150 (MQ=255)
                                                                    aGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGc                                                                              >  1:4287/1‑150 (MQ=255)
                                                                    aGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGc                                                                              >  2:1307873/1‑150 (MQ=255)
                                                                     gAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCt                                                                             >  2:1795479/1‑150 (MQ=255)
                                                                     gAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCt                                                                             >  2:1009668/1‑150 (MQ=255)
                                                                     gAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATAGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCt                                                                             >  2:286833/1‑150 (MQ=255)
                                                                        aCTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGc                                                                          >  2:1135033/1‑150 (MQ=255)
                                                                           gggACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCtt                                                                       <  1:35386/150‑1 (MQ=255)
                                                                              aCTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGc                                                                    <  2:820065/150‑1 (MQ=255)
                                                                               cTGAGCGCACTCTCCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTATCAGTCTGCCGCAAAATAAGGCTGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATAACTGGCGCTGGCATTTTATTGCTGGCCTTTGcc                                                                   <  2:1179541/150‑1 (MQ=255)
                                                                               cTGAGCGCACTCACCGCGCTGGTATTCAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGcc                                                                   <  1:649119/150‑1 (MQ=255)
                                                                               cTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGcc                                                                   <  2:644905/150‑1 (MQ=255)
                                                                               cTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGcc                                                                   >  2:75574/1‑150 (MQ=255)
                                                                                   gcgcACTCACCTCGCTGGTATTAAGCTCAATGATGGGCGCGGGTGTTATCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCAACGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGACAttg                                                               >  1:1654070/1‑148 (MQ=255)
                                                                                   gcgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGc                                                               <  2:1637397/150‑1 (MQ=255)
                                                                                   gcgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGc                                                               >  1:554537/1‑150 (MQ=255)
                                                                                   gcgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGc                                                               <  2:1745938/150‑1 (MQ=255)
                                                                                    cgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCt                                                              >  2:1248387/1‑150 (MQ=255)
                                                                                    cgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCt                                                              <  1:1288212/150‑1 (MQ=255)
                                                                                     gcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTg                                                             <  2:1582413/150‑1 (MQ=255)
                                                                                      cACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGa                                                            >  2:598491/1‑150 (MQ=255)
                                                                                      cACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGa                                                            >  1:247392/1‑150 (MQ=255)
                                                                                        cTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATc                                                          <  2:626522/150‑1 (MQ=255)
                                                                                          ctccGCGCTGGTATTAAGCTCAATGCGGGGCGCGGGTGTTTTCAGTCTGCCGCATATTATGGCGGCAGTTGCCAGCCCGGCAGGACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCTTGCTGATCCt                                                        <  2:147028/148‑1 (MQ=255)
                                                                                            ccGCGCTGGTATTAAGCTCAATGTTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTcc                                                      >  1:796292/1‑149 (MQ=255)
                                                                                               cgcTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCAcgc                                                   <  2:719812/150‑1 (MQ=255)
                                                                                                   ggTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCAt                                               >  2:837664/1‑150 (MQ=255)
                                                                                                   ggTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCAt                                               >  1:1448910/1‑150 (MQ=255)
                                                                                                   ggTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGAAGTTGCCAGCCCGGCAGCCCTGCTCATCTGCTGGGTGATTACTGGCTCTTGCATTTTATTGCTGGCCGTTGCCATGCTGATACTCACGAGCAt                                               >  1:1746714/1‑150 (MQ=255)
                                                                                                    gTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCAtt                                              >  2:415826/1‑150 (MQ=255)
                                                                                                    gTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGATGGCCTATGCGATGCTTATCCTCACGCGCAta                                              >  2:101260/1‑149 (MQ=255)
                                                                                                      aTTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCg                                            >  2:109679/1‑150 (MQ=255)
                                                                                                       ttAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAAAAAGGCGGCAGATGCCCGCCCGCCAGCACTGCTCCTCGGCGGGGGTTTTACTCGCCCTGGCATTTTAAAGCTGGCCATAGCCAAGCTGATCCTCACGCGCATACgg                                           >  2:1755041/1‑149 (MQ=255)
                                                                                                        tAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCGAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTc                                          <  1:178533/150‑1 (MQ=255)
                                                                                                        tAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTc                                          >  2:1596694/1‑150 (MQ=255)
                                                                                                        tAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTc                                          >  1:1823442/1‑150 (MQ=255)
                                                                                                        tAAGCGCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTc                                          <  1:990589/150‑1 (MQ=255)
                                                                                                         aaGCTCAATGCTGGGCGCGGGAGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCATTTGCCATGCTGATCCTCACGCGCATTCGTcc                                         >  1:392846/1‑150 (MQ=255)
                                                                                                         aaGCTAAATGCTGGGCGCGGGTGTTTTCAGTATGGCGCAAAATTTGGCGGCAGTTGCCTGCCCTGCAGCACTGCTCTTGGGGTGGGGTATTACTGGCGCTGTCATTTTATTGCTGGCCTTTGCCATGCTGATGCTCACGCGCATTCGTcc                                         <  1:708652/150‑1 (MQ=255)
                                                                                                          aGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTccc                                        <  1:996296/150‑1 (MQ=255)
                                                                                                            cTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGa                                      >  2:756346/1‑150 (MQ=255)
                                                                                                            cTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGa                                      <  1:1348475/150‑1 (MQ=255)
                                                                                                              cAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAAc                                    >  2:847686/1‑150 (MQ=255)
                                                                                                                aTGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGCTCCTCACGCGCATTCGTCCCGAACtt                                  >  2:1527552/1‑150 (MQ=255)
                                                                                                                    tGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcg                              <  2:1532148/150‑1 (MQ=255)
                                                                                                                     gggCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcgg                             >  1:669609/1‑150 (MQ=255)
                                                                                                                      ggCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggc                            <  1:1281177/150‑1 (MQ=255)
                                                                                                                        cgcgGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggcgg                          <  2:1072387/150‑1 (MQ=255)
                                                                                                                            ggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAACACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATc                      <  1:1823153/150‑1 (MQ=255)
                                                                                                                                ttatCAGTCTGCCGAAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCGGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTa                  <  1:1597509/147‑1 (MQ=255)
                                                                                                                                 tttCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCCCTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGCTCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTAc                 >  1:363897/1‑150 (MQ=255)
                                                                                                                                 tttCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggcgg                          >  1:116488/1‑141 (MQ=255)
                                                                                                                                 tttCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggcgg                          <  2:116488/141‑1 (MQ=255)
                                                                                                                                 ctggcaacTGCCGCCATATTTTGCGGCAGTTGCCCGCCCGGCAGCGCTGCTCATCGGCTGGGGGATTACTGGCGCTGGCATTGTATTGCTGGCCTTTGCCATGCTGATCCTCCCGCGCATTCGTCCCGACCTTGACGGCGGTATCTGTAc                 >  1:260421/8‑150 (MQ=255)
                                                                                                                                     caacTGCCGCCATATTTTGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTAt             >  2:1142864/4‑150 (MQ=255)
                                                                                                                                       acTGCCGCCATATTTTGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCCCGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGc           >  2:1067877/2‑150 (MQ=255)
                                                                                                                                        cTGCCGCCATATTTTGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGcc          >  2:829053/1‑150 (MQ=255)
                                                                                                                                           ccGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCcgc       >  1:606205/1‑150 (MQ=255)
                                                                                                                                           ccGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCAATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCcgc       >  2:1590168/1‑150 (MQ=255)
                                                                                                                                            cGCAAAATAGGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCcgcg      <  2:1772696/150‑1 (MQ=255)
                                                                                                                                               aaaaTATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAg   >  1:551592/1‑150 (MQ=255)
                                                                                                                                                aaaTATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAgg  <  2:1300101/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGG  >  NC_000913/1679496‑1679789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑