Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,707,029 A→G 7.6% V37V (GTA→GTG) ‡ rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,0290AG 7.6% 247.5 / 8.6 93V37V (GTA→GTG) ‡rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (37/47);  new base G (4/3);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

TGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGG  >  NC_000913/1706880‑1707177
                                                                                                                                                      |                                                                                                                                                    
tGAACACCATTCCCGTGCGTATCATTCCCGTGGACCCCCATGCTTCAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                                                      >  1:1239453/1‑150 (MQ=255)
 gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGTAc                                                                                                                                                     >  2:1272771/1‑150 (MQ=255)
   acacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc                                                                                                                                                   >  2:98224/1‑150 (MQ=255)
    cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                  <  1:490310/150‑1 (MQ=255)
    cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                  >  1:1436308/1‑150 (MQ=255)
      ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                                                >  2:218955/1‑150 (MQ=255)
      ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCCGCATCCATCCACCGGAGATGAAAACACAGTCCAACGGTACACCCCTCCGCCAGGTACCCCTg                                                                                                                                                >  1:90589/1‑150 (MQ=255)
       cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCCCCCGGTTCCCCggg                                                                                                                                               >  1:1751042/1‑150 (MQ=255)
        aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTGCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                                              <  1:527167/150‑1 (MQ=255)
           cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa                                                                                                                                           >  2:636207/1‑150 (MQ=255)
           cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCa                                                                                                                                           <  2:678561/150‑1 (MQ=255)
             cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                         <  2:416817/150‑1 (MQ=255)
              gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAggg                                                                                                                                        <  1:833558/150‑3 (MQ=255)
              gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAggg                                                                                                                                        <  1:707777/150‑3 (MQ=255)
               tGCGTATCATTCCCGGGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGt                                                                                                                                       <  2:192501/150‑1 (MQ=255)
                gCGTATCCTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCGGCGATTTCTACGGCGGCAGCCATGCTCCGGAGATGAAACCCCAGTCCATCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                                                      <  1:49948/150‑1 (MQ=255)
                 cGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                                                     <  2:1692877/150‑1 (MQ=255)
                   tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                   <  2:1486667/150‑1 (MQ=255)
                   tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTAAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                   <  2:1393265/150‑1 (MQ=255)
                    aTCATTCCCGTGGAACACCATGCTTATGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                  <  2:1225414/150‑1 (MQ=255)
                    aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCAGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                  >  1:969058/1‑150 (MQ=255)
                     tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGtt                                                                                                                                 <  2:1444769/150‑1 (MQ=255)
                       aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                               <  1:1626592/150‑1 (MQ=255)
                       aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCATGGCGGAGCGTGTTGTTAt                                                                                                                               >  2:159610/1‑150 (MQ=255)
                        ttCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAtt                                                                                                                              >  2:21128/1‑150 (MQ=255)
                           ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTccc                                                                                                                           >  1:1217559/1‑149 (MQ=255)
                             gTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACt                                                                                                                         <  1:1194332/150‑1 (MQ=255)
                               ggAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGa                                                                                                                       >  2:1264525/1‑150 (MQ=255)
                               ggAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCACCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGa                                                                                                                       >  1:412812/1‑150 (MQ=255)
                                taaCACCATTCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACTGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaa                                                                                                                      <  2:1453222/149‑1 (MQ=255)
                                gAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAAATAAAATCTGTGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGa                                                                                                                       <  2:874761/150‑1 (MQ=255)
                                     ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc                                                                                                                 >  2:1572166/1‑150 (MQ=255)
                                      cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                                >  1:1070747/1‑150 (MQ=255)
                                            tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                          >  2:37619/1‑150 (MQ=255)
                                            tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTgg                                                                                                          >  1:469417/1‑150 (MQ=255)
                                              aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcg                                                                                                        <  1:378763/150‑1 (MQ=255)
                                               gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCTTCCATCCACCGGAGATGAACCCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                       <  2:78776/150‑1 (MQ=255)
                                                 tATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                                                                     <  1:98224/150‑1 (MQ=255)
                                                     ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGTGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                                                 <  1:1072706/150‑1 (MQ=255)
                                                        tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                                                                              <  1:568720/150‑1 (MQ=255)
                                                         gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                                                                             <  1:869155/150‑1 (MQ=255)
                                                         gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                                                                             <  2:1219022/150‑1 (MQ=255)
                                                           aTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTACAACGGAACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTAATTCCACAGAAACAGCATATTGGCGCTGAAGGTGAGtt                                                                                           >  1:953177/1‑150 (MQ=255)
                                                            ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTCGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtg                                                                                          >  1:1162926/1‑150 (MQ=255)
                                                                 caaaaaaTAAACTCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg                                                                                      <  2:705605/149‑1 (MQ=255)
                                                                    aaaataaaatCTGTGATTTCAACTGCGGCATACATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa                                                                                   <  2:1780893/150‑1 (MQ=255)
                                                                     aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                                                  <  1:1074827/150‑1 (MQ=255)
                                                                         aaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCACGTACCCCTGGCGCACCGTGATCTTATTACACTGAAACAGCATATTGGCCCAGAAGGTCAGTTGTGCGTTAGAGta                                                                              >  1:1819509/1‑149 (MQ=255)
                                                                          aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAGTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGGGTTTGCGTCg                                                                             >  1:1580672/1‑150 (MQ=255)
                                                                            atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                           <  1:1777782/150‑1 (MQ=255)
                                                                            atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                           <  1:1473093/150‑1 (MQ=255)
                                                                               tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGAACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGAGTTAGCGTCGGCGAt                                                                        >  2:217969/1‑150 (MQ=255)
                                                                                    tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                   >  1:1768595/1‑150 (MQ=255)
                                                                                       cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTTTTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTTTGCGTTTGCGTCGGCGATAAAGTAtt                                                                >  2:1248019/1‑150 (MQ=255)
                                                                                            gcggcATCCCTCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGcgcg                                                           <  2:90589/150‑1 (MQ=255)
                                                                                             cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGAAAAAGGATTGCGCgg                                                          >  2:1568559/1‑150 (MQ=255)
                                                                                                    catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGGCGCCGGGCGCCGCGTTTTGGTATTCCCCTGCAACAGCAGAGTGGGGGTGCAGGGGGGTTGGGGGTTGGCGGGGGGGGTCAAGGTTTGGGCGGCccgccg                                                   >  2:787736/1‑150 (MQ=255)
                                                                                                      tccaCCTGAGATGAAAACCCAGTCCAACGGTCCACCCCTGCGCCACGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTTTTGCGCGGCCAGCCGCt                                                 <  2:1579033/150‑1 (MQ=255)
                                                                                                           cGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccc                                            <  2:1441133/150‑1 (MQ=255)
                                                                                                            ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCg                                           <  1:1368008/150‑1 (MQ=255)
                                                                                                              agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                         <  2:950754/150‑1 (MQ=255)
                                                                                                                aTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                       <  2:1288466/150‑1 (MQ=255)
                                                                                                                 tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTTCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGCCGCTTACCCGTGGTc                                      >  1:855709/1‑150 (MQ=255)
                                                                                                                 tGAAAACCCAGTCCAACGGTACACCCCTGCCCCCGGTACCCCTGGCGCCGCGGTTTTTTATTGCCCTGAAACAGCATAATGGCGCTGAAGGTGAGTTGTGCGTTTTCGTCCGCGATAAAGTATTTCGCGGCCACCCGCTTTCCCGTGGta                                      >  2:1370329/1‑149 (MQ=255)
                                                                                                                    aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcgcg                                   <  1:31223/150‑1 (MQ=255)
                                                                                                                     atcccAGTCCAACGGTACACCCCTGCGCCAGGGACCCCTGTCGCAGCGTTTTGTTATTCAACTGAAACAGCATATTGGCGCTGATGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCgg                                  <  2:469417/148‑1 (MQ=255)
                                                                                                                      aCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                 <  1:583721/150‑1 (MQ=255)
                                                                                                                      aCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTTTTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTCTTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                 <  1:1366039/150‑1 (MQ=255)
                                                                                                                      aCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGGCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                 <  1:1358553/150‑1 (MQ=255)
                                                                                                                       cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCa                                >  1:1453992/1‑150 (MQ=255)
                                                                                                                       cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACAGAAACAGCATATTGGCGCAGAAGGTGAGTTGTGCGTTTGCGTCGGCGATAAAGTATTTCGCGGCCAACCGCTTACCCCTGGTCCCGGCa                                >  2:546173/1‑150 (MQ=255)
                                                                                                                         cAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCaaa                              <  1:1453195/150‑1 (MQ=255)
                                                                                                                           gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAt                            <  1:1284329/150‑1 (MQ=255)
                                                                                                                            tCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtg                           <  2:1751042/150‑1 (MQ=255)
                                                                                                                            tCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTTGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTAGCGGCCACCCGCTTACCCGTGGTCGCCGCAAAAtg                           >  2:1314382/1‑150 (MQ=255)
                                                                                                                              cAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgct                         >  2:1210753/1‑150 (MQ=255)
                                                                                                                                aCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctgc                       <  1:1264525/150‑1 (MQ=255)
                                                                                                                                 cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                      <  2:1572324/150‑1 (MQ=255)
                                                                                                                                 cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                      <  1:1572166/150‑1 (MQ=255)
                                                                                                                                 cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCCTCGGCGATAAACTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                      >  2:1688272/1‑150 (MQ=255)
                                                                                                                                   gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTg                    <  2:977029/150‑1 (MQ=255)
                                                                                                                                       acCCCTGCGCCAGGTCCCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCa                <  1:315481/150‑1 (MQ=255)
                                                                                                                                        ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc               >  1:61181/1‑150 (MQ=255)
                                                                                                                                        ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACACGTGGTCGCGGCAAAAAGCTGCCTGTTCAc               >  2:810565/1‑150 (MQ=255)
                                                                                                                                        ccccTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc               <  1:1272771/150‑1 (MQ=255)
                                                                                                                                        acgcTGCGCCAGGGGTACCTGGCGCAGGGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAAGCTGCCTGTTCAc               >  1:1764769/4‑150 (MQ=255)
                                                                                                                                          ccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc             >  2:709420/1‑150 (MQ=255)
                                                                                                                                           cTGCGCCAGGGGTACCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCCGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgcg            >  2:1415716/1‑150 (MQ=255)
                                                                                                                                            tGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc           >  2:930995/1‑150 (MQ=255)
                                                                                                                                             gcgcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGcc          <  1:1825898/150‑1 (MQ=255)
                                                                                                                                                ccAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAc       >  1:697410/1‑150 (MQ=255)
                                                                                                                                                    gTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCg   >  1:288462/1‑150 (MQ=255)
                                                                                                                                                    gTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCg   >  1:813604/1‑150 (MQ=255)
                                                                                                                                                     tACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCTGCAAAATGCTGCCTGTTCACGCGCCCACCTCgg  <  1:1617541/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGG  >  NC_000913/1706880‑1707177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: