Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,764,694 G→C 9.2% intergenic (‑308/+19) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,6940GC 9.2% 286.3 / 17.3 96intergenic (‑308/+19)sufA/rydBFe‑S cluster assembly protein/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (43/44);  new base C (2/7);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

GAATTAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATAT  >  NC_000913/1764545‑1764841
                                                                                                                                                     |                                                                                                                                                   
gAATTAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCg                                                                                                                                                     >  1:1451190/1‑150 (MQ=255)
   ttAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGTAGAATTATGGCTGTGATGATAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGaca                                                                                                                                                  >  1:889483/1‑150 (MQ=255)
      ggTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGTTAAAAAAGTTCAGCGTTCAGAAATAAGAAAACCCTTAAGTATGTGCGACACAg                                                                                                                                               >  2:890444/1‑150 (MQ=255)
      ggTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAg                                                                                                                                               >  1:1354867/1‑150 (MQ=255)
        tATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGc                                                                                                                                             <  2:1043873/150‑1 (MQ=255)
           ctcATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTa                                                                                                                                          >  2:1823125/1‑150 (MQ=255)
           ctcATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTa                                                                                                                                          <  2:476999/150‑1 (MQ=255)
            tgctccttAGATAATAACCATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTaa                                                                                                                                         >  2:1778708/7‑150 (MQ=255)
            tcATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTaa                                                                                                                                         >  2:731284/1‑150 (MQ=255)
            tcATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTaa                                                                                                                                         >  2:730681/1‑150 (MQ=255)
             cATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAg                                                                                                                                        <  2:1473988/150‑1 (MQ=255)
              cttGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTTTGATGAAAAATAATAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCATCTTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                                                                       <  2:1130641/149‑1 (MQ=255)
              ctccttAGATAATAACCATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                                                                       >  2:382013/5‑150 (MQ=255)
              ctccttAGATAATAACCATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                                                                       >  2:1084646/5‑150 (MQ=255)
              aTTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                                                                       >  2:597192/1‑150 (MQ=255)
               ttGTTAGATAATGTTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                                                                      <  1:507942/150‑1 (MQ=255)
               ttGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                                                                      <  1:445482/150‑1 (MQ=255)
               ttGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                                                                      <  2:1581583/150‑1 (MQ=255)
                     gATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTa                                                                                                                                >  1:72187/1‑150 (MQ=255)
                     gATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGttttct                                                                                                                                <  2:815624/150‑4 (MQ=39)
                      aTAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAc                                                                                                                               >  2:1615876/1‑150 (MQ=255)
                       tattGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                                                                              <  2:76437/147‑1 (MQ=255)
                       tAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                                                                              <  1:1071063/150‑1 (MQ=255)
                       tAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                                                                              >  1:1250008/1‑150 (MQ=255)
                         aTGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcccc                                                                                                                            >  2:1585092/1‑150 (MQ=255)
                          tGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                                                                           <  1:734845/150‑1 (MQ=255)
                           ggTTATTATCTAAGGAGCATTAAGGCTTTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                                                                          <  2:1287056/150‑1 (MQ=255)
                           agTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                                                                          >  1:821149/2‑150 (MQ=255)
                                ttatCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgc                                                                                                                     >  1:1391622/1‑150 (MQ=255)
                                 tatCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgcg                                                                                                                    <  1:619094/150‑1 (MQ=255)
                                  atCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgcgc                                                                                                                   <  1:652716/150‑1 (MQ=255)
                                  atCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgcgc                                                                                                                   <  1:1107219/150‑1 (MQ=255)
                                   tCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCt                                                                                                                  >  2:1717432/1‑150 (MQ=255)
                                       aGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                                                                              >  1:1420567/1‑150 (MQ=255)
                                        ggAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtct                                                                                                             >  2:394739/1‑150 (MQ=255)
                                          aGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                                                                           >  2:1044686/1‑150 (MQ=255)
                                            cATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCgg                                                                                                         <  2:421343/150‑1 (MQ=255)
                                             aTTAAGGCTGTTATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATTAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGTGTTTCTACCCCATCCGGCGCTTATCTCCGGc                                                                                                        <  1:1011182/150‑1 (MQ=255)
                                                aaGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAct                                                                                                     <  2:22398/150‑1 (MQ=255)
                                                    cTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGAGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGAGACACAGTCTTAAGTGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                                                                                 >  2:129713/1‑150 (MQ=255)
                                                         atgaAAAAGAAAATGTTGTATTGAAAATGACTATTTAAGAGATAGGTTAATAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACTCAGGATTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCTGCACTATCAGTGGc                                                                                            <  2:1775634/150‑1 (MQ=255)
                                                            aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTa                                                                                         >  1:737200/1‑150 (MQ=255)
                                                               aagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAACAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGAGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGct                                                                                      <  2:1158390/150‑1 (MQ=255)
                                                                agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGAGCTTATCTCCGGCACTCTCAGTGGCTTAGcta                                                                                     >  2:506813/1‑149 (MQ=255)
                                                                 gaaaaagTTGAATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctct                                                                                    <  2:132283/150‑1 (MQ=255)
                                                                     aagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGaa                                                                                <  2:72187/150‑1 (MQ=255)
                                                                       gTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGAAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCACATCCGGCGATTATCTCCGGCACTCTCAGTGGCTTAGCTCTTTAAgg                                                                              >  1:582577/1‑150 (MQ=255)
                                                                           tATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCg                                                                          <  2:816471/150‑1 (MQ=255)
                                                                            aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACACCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGa                                                                         >  1:440037/1‑150 (MQ=255)
                                                                              tcaaaaTGACTATTTAAGATATTGGTAAAAAAGTGCACCGTTCTTAAATAATAAAACCCTTAAGTCTGTGCCACACTGGCTTATGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                                                                       <  2:258648/148‑1 (MQ=255)
                                                                              tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                                                                       >  1:1097800/1‑150 (MQ=255)
                                                                               gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATaa                                                                      <  2:1107311/150‑1 (MQ=255)
                                                                                aaaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAg                                                                     <  1:1479124/150‑1 (MQ=255)
                                                                                aaaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAg                                                                     <  1:1486554/150‑1 (MQ=255)
                                                                                   aTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat                                                                  >  1:18041/1‑150 (MQ=255)
                                                                                     gACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa                                                                <  1:399917/150‑1 (MQ=255)
                                                                                      aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataat                                                               >  1:832141/1‑150 (MQ=255)
                                                                                         aTTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGTGCGATAAGAATAAtcta                                                            >  2:1124903/1‑149 (MQ=255)
                                                                                            tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATa                                                         <  1:753910/150‑1 (MQ=255)
                                                                                             aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCATTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGTATAATCTCATaa                                                        >  2:1163389/1‑150 (MQ=255)
                                                                                              agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaaa                                                       >  1:218918/1‑150 (MQ=255)
                                                                                                    aGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAc                                                 <  1:1721271/150‑1 (MQ=255)
                                                                                                        aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgc                                             <  2:1250008/150‑1 (MQ=255)
                                                                                                           aaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                          <  2:1586822/150‑1 (MQ=255)
                                                                                                           aaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                          <  2:57980/150‑1 (MQ=255)
                                                                                                              gTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGtttt                                       <  1:1142896/150‑1 (MQ=255)
                                                                                                               tGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGGAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTa                                      <  2:889483/150‑1 (MQ=255)
                                                                                                                gCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTTTCTCCGGCACTCTCAGTGGCGTAGCTCTTGAAGGGGCGATAAGAATACTCTCATAAAGCTAACCCGCCGTTTTaa                                     >  1:1647527/1‑150 (MQ=255)
                                                                                                                 cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                    <  1:1403980/150‑1 (MQ=255)
                                                                                                                 cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                    <  2:821149/150‑1 (MQ=255)
                                                                                                                 cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                    <  2:185578/150‑1 (MQ=255)
                                                                                                                      ttCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACaaa                               >  2:1632488/1‑150 (MQ=255)
                                                                                                                       tCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGATTAATCTCATAAAGCTAACCCGCCGTTTTAACACAAAc                              >  2:1076139/1‑150 (MQ=255)
                                                                                                                        ctgAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACt                             >  2:17144/3‑150 (MQ=255)
                                                                                                                        cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACaaaat                             >  2:986912/1‑148 (MQ=255)
                                                                                                                         aGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCGTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTg                            >  1:189824/1‑150 (MQ=255)
                                                                                                                          gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGc                           >  2:1563963/1‑150 (MQ=255)
                                                                                                                              tAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                       <  2:424512/150‑1 (MQ=255)
                                                                                                                              tAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                       <  2:911198/150‑1 (MQ=255)
                                                                                                                              tAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                       >  1:955058/1‑150 (MQ=255)
                                                                                                                              tAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCACATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                       <  1:627719/150‑1 (MQ=255)
                                                                                                                                aGAAAACCCTTCAGTCTGAGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTAATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAg                     <  2:412868/150‑1 (MQ=255)
                                                                                                                                 taaaaCCCTTAAGTCTGGGCGACACCGGCTTATGGGTTTATACCCCATCCGGCCGTTATCTCCGGCACTCTAAGTGGCTTAGCTCTTGAAGGGGCGATAAGATTAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGt                    <  1:1778708/149‑1 (MQ=255)
                                                                                                                                     aCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtatta                >  1:575539/1‑150 (MQ=255)
                                                                                                                                      cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtagtat               >  2:1191403/1‑150 (MQ=255)
                                                                                                                                        cTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttt             >  1:431376/1‑150 (MQ=255)
                                                                                                                                          tAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGTTGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttttt           <  1:890444/150‑1 (MQ=255)
                                                                                                                                          tAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTTACACAAACTGCGATTAGTATTAttttt           >  2:866269/1‑150 (MQ=255)
                                                                                                                                           aaGTCTGTGCGACTCAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGTCGATAAGAATAATCTGATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg          <  1:1399809/150‑1 (MQ=255)
                                                                                                                                           aaGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGTCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg          <  1:1773524/150‑1 (MQ=255)
                                                                                                                                           aaGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg          <  1:761492/150‑1 (MQ=255)
                                                                                                                                           aaGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg          <  2:1600067/150‑1 (MQ=255)
                                                                                                                                            aGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGa         >  2:549513/1‑150 (MQ=255)
                                                                                                                                              tCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAAc       <  1:731284/150‑1 (MQ=255)
                                                                                                                                                  tgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAata   <  1:1657768/150‑1 (MQ=255)
                                                                                                                                                  tgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAata   <  1:1498455/150‑1 (MQ=255)
                                                                                                                                                   gCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTACCTCTTGAAGGGGCGATAAGAATAATCTCTTAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTTTTTTTGAACAatat  >  1:1669406/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAATTAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATAT  >  NC_000913/1764545‑1764841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: