Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,795,227 A→T 9.5% intergenic (‑75/+26) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,795,2270AT 9.5% 295.5 / 19.4 116intergenic (‑75/+26)btuC/ihfAvitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (47/58);  new base T (5/6);  total (52/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 119 total aligned reads displayed.

CAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCC  >  NC_000913/1795078‑1795369
                                                                                                                                                     |                                                                                                                                              
cagcagCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGGCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCa                                                                                                                                                <  1:1437333/150‑1 (MQ=255)
  gcagcaTCAAAACTGACAGGCATAATAACCAGCGTATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCATCGACAGTGAAAAGAAAAAAGGCCGCaga                                                                                                                                              >  2:142268/1‑150 (MQ=255)
   cagcgTCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCCGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCagcg                                                                                                                                             >  1:730830/1‑148 (MQ=255)
      caTCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCgg                                                                                                                                          <  1:226973/150‑1 (MQ=255)
      caTCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCgg                                                                                                                                          <  1:1565924/150‑1 (MQ=255)
         cAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTAATGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCt                                                                                                                                       >  2:1177271/1‑150 (MQ=255)
           aaaCTGACAGGCATAATACCCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGAATCCGTTCTGCTGATGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttt                                                                                                                                     <  2:650313/150‑1 (MQ=255)
            aaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCtttt                                                                                                                                    >  2:1080666/1‑150 (MQ=255)
             aCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAGAAGGCCGCAGAGCGGCCttttt                                                                                                                                   <  2:1097990/150‑1 (MQ=255)
             aCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttt                                                                                                                                   <  2:355671/150‑1 (MQ=255)
             aCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttttt                                                                                                                                   <  2:3984/150‑1 (MQ=255)
              cTGACAGGCATAAAAACCAGAGAATATTTTGTCGCTGTTGTTGGCGGGAAAGTGTAAGAATGGTATCCGTTCTGCTGAAGGGTCATGGCGGTGATTTTACTGTGACTCTTCGCCAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTtta                                                                                                                                  <  2:1095173/150‑1 (MQ=255)
               tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttttc                                                                                                                                 <  1:13233/150‑3 (MQ=255)
               tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttag                                                                                                                                 <  1:11007/150‑1 (MQ=255)
               tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttag                                                                                                                                 >  1:737790/1‑150 (MQ=255)
               tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTGCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttttc                                                                                                                                 <  2:929384/150‑3 (MQ=255)
                gACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttttct                                                                                                                                <  1:1805108/150‑4 (MQ=255)
                 aCAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtt                                                                                                                               <  2:29731/150‑1 (MQ=255)
                  cAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtta                                                                                                                              <  2:39964/150‑1 (MQ=255)
                  cAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGATGGCGGGCAAGTGTCAGCATGGAATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGTTGACTCTTCGACAGTGAAAAGAAAAAAGTACGCAGACCGGCCTTTttagtta                                                                                                                              >  1:1674692/1‑150 (MQ=255)
                   aGGCATAATACCCCGCGAATATTATGTCGCTGTTGTAGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagttag                                                                                                                             <  1:347671/150‑1 (MQ=255)
                   aGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagttag                                                                                                                             >  2:1531892/1‑150 (MQ=255)
                    ggCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCttttttcttttc                                                                                                                            <  2:219803/150‑8 (MQ=255)
                    ggCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGa                                                                                                                            >  1:1402258/1‑150 (MQ=255)
                     gCATAATTACGACCGAATATTTTGTCGCTGTTGTTGGAGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTTATTATACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGAt                                                                                                                           <  2:1633580/150‑1 (MQ=255)
                      cATAATAACCAGCGATTATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAACGGCCGCAGAGCGGCCTTTTTAGTTAGATc                                                                                                                          <  1:445074/150‑1 (MQ=255)
                      cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATc                                                                                                                          <  2:278247/150‑1 (MQ=255)
                      cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATc                                                                                                                          >  2:149787/1‑150 (MQ=255)
                      cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATc                                                                                                                          <  1:1443249/150‑1 (MQ=255)
                       ataataACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGACATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCa                                                                                                                         <  1:80796/150‑1 (MQ=255)
                        taataaGCAGCTTATATTTTGTTGCTGTTGTTGTCGGGTAATTTTTATCCAGGTATCCCTTCTTCTGAAGAGTCACGGCGTTGTATTTACGGGGACTCATCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAg                                                                                                                        <  1:110146/150‑1 (MQ=255)
                          ataaCCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGAt                                                                                                                      <  2:483668/150‑1 (MQ=255)
                          ataaCCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGAt                                                                                                                      <  1:905295/150‑1 (MQ=255)
                                aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCg                                                                                                                >  2:523261/1‑150 (MQ=255)
                                 gCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGt                                                                                                               >  1:42308/1‑150 (MQ=255)
                                       atTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTgg                                                                                                         >  2:1234227/1‑150 (MQ=255)
                                        ttttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTTATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTggg                                                                                                        <  2:1081696/150‑1 (MQ=255)
                                        ttttGGCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTggg                                                                                                        <  1:739947/150‑1 (MQ=255)
                                          ttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCg                                                                                                      <  2:910762/150‑1 (MQ=255)
                                          ttGTCGCTGGTGTCGGCGCGCAAGTGTCCGCACGGCATCCCTGCTGCCGAAGCGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGACACGAAAAAAGGCCGAAGAGCGGCCTTTTTAGTTAGCTCAGATTACTCGTCTTTGGGCg                                                                                                      <  2:267948/150‑1 (MQ=255)
                                           tGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCGGATTACTCGTCTTTGGGCGa                                                                                                     <  1:874739/150‑1 (MQ=255)
                                               gCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGGCATGGCGTTGAGTTTACGGGGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCg                                                                                                 <  1:251322/150‑1 (MQ=255)
                                                    tgttgGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTc                                                                                            >  1:1582159/1‑150 (MQ=255)
                                                    tgttgGCGGGCAAGTGTCAGCATGGTATCCGTTATGCTGAAGTGTCATGTCGTTGATTTTACGGAGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGCCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTc                                                                                            >  2:1591287/1‑150 (MQ=255)
                                                     gttgGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCg                                                                                           <  1:1569866/150‑1 (MQ=255)
                                                     gttgGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCg                                                                                           <  1:578878/150‑1 (MQ=255)
                                                     gttgGAGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGCCATGGCGTAGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTGTTTTGTTGGATCAGATTACTCGTGTTTGGGCGAAGCGTTTTCg                                                                                           >  2:21253/1‑150 (MQ=255)
                                                        ggCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAcc                                                                                        >  1:954674/1‑150 (MQ=255)
                                                           gggCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCgg                                                                                     <  1:609314/150‑1 (MQ=255)
                                                               aaGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGGGACTCTTCGACAGTGAAAAGAAAACAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCttt                                                                                 <  1:142268/150‑1 (MQ=255)
                                                                  tgtCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTa                                                                              <  2:1556482/150‑1 (MQ=255)
                                                                   gtCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTAGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTaa                                                                             <  1:905368/150‑1 (MQ=255)
                                                                     cAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACt                                                                           <  1:486889/150‑1 (MQ=255)
                                                                       gCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACtta                                                                         >  2:557321/1‑149 (MQ=255)
                                                                        cATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCt                                                                        >  1:1318701/1‑150 (MQ=255)
                                                                        atgggTTTCCGTTCTGGGTATGTGTCTTGGCGTTGATTTTACGGTTACTCTTCTACAGTGAAAATAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCt                                                                        <  2:754348/147‑1 (MQ=255)
                                                                         aTGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTg                                                                       <  1:839755/150‑1 (MQ=255)
                                                                         aTGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTg                                                                       <  1:1718257/150‑1 (MQ=255)
                                                                              aTCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCgg                                                                  >  2:1699717/1‑150 (MQ=255)
                                                                               tCCGTTCTGCTGAAGTGTCATGGCGTGGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGAGCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCggg                                                                 <  2:223219/150‑1 (MQ=255)
                                                                               tCCCTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCggg                                                                 <  2:774970/150‑1 (MQ=255)
                                                                                      tgctgAAGTGTCATGGCGTTGATTTTCCGGTGACTCTTCGACAGTGAAAACAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTGTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAg                                                          <  1:345751/150‑1 (MQ=255)
                                                                                      tgctgAAGTGTCATGGCGTTGATTTTAGGGTGACTCTTCGACAGTGAAATGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAg                                                          <  1:120850/150‑1 (MQ=255)
                                                                                        ctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGTGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGt                                                        >  2:183370/1‑150 (MQ=255)
                                                                                         tgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGta                                                       >  1:308277/1‑149 (MQ=255)
                                                                                         tgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTc                                                       <  1:687627/150‑1 (MQ=255)
                                                                                               gtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcg                                                 >  1:1164747/1‑150 (MQ=255)
                                                                                                         tGATTTTACGGGGACTCATCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTg                                       <  1:1495963/150‑1 (MQ=255)
                                                                                                             tttACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGGGCTGTAAt                                   >  1:641776/1‑150 (MQ=255)
                                                                                                              ttACGGGGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGGCACCACGCGCCGGGCTGTAATg                                  <  2:1113908/150‑1 (MQ=255)
                                                                                                               tACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATgg                                 <  1:1152681/150‑1 (MQ=255)
                                                                                                                aCGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCAACCCGGCTTTTAAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATggg                                <  2:411311/150‑1 (MQ=255)
                                                                                                                aCGGCGACTCTTCGACAGTGAAAAGAAAAAAGCCCCCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCGGTAATggg                                <  2:270498/150‑1 (MQ=255)
                                                                                                                 cGGTGCCTCTGCGACAGTGAACAGACAAACGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTCACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGa                               <  1:259960/150‑1 (MQ=255)
                                                                                                                  ggTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGaa                              <  2:594787/150‑1 (MQ=255)
                                                                                                                  ggTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGaa                              <  2:1164747/150‑1 (MQ=255)
                                                                                                                  ggTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCATGCTGTAATGGGaa                              <  2:1470777/150‑1 (MQ=255)
                                                                                                                   gTGACTCATCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGTCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGGTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAat                             <  2:1582507/150‑1 (MQ=255)
                                                                                                                     gACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAatat                           >  2:737023/1‑150 (MQ=255)
                                                                                                                       ctctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGTTCACCACGCGCCGTGATGTAATGGGAATATcc                         >  1:143133/1‑150 (MQ=255)
                                                                                                                         ctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTc                       >  2:6619/1‑150 (MQ=255)
                                                                                                                            cGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGcc                    <  2:365887/150‑1 (MQ=255)
                                                                                                                                 gTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAACCGGCTTTTTAACTTCTGCCCTGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGttt               >  1:1567516/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCgg           >  2:188978/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCgg           >  2:67244/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGACGTGCTGTAATGGGAATATCCTCTCCCGTTTTCgg           >  2:287563/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaAACGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTACgg           >  2:551286/1‑150 (MQ=255)
                                                                                                                                      aactaaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACGCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCggg          >  1:265666/5‑150 (MQ=255)
                                                                                                                                       agaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCCCGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt         >  2:1696029/1‑150 (MQ=255)
                                                                                                                                       agaaaaAAGGCCGCAGAGCGGCCGTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCAGGCTTTTTAACTTCTGACCGGGTCGGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt         >  2:747811/1‑150 (MQ=255)
                                                                                                                                       actaaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt         >  2:1657136/4‑150 (MQ=255)
                                                                                                                                        gaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt        >  1:1055345/1‑150 (MQ=255)
                                                                                                                                        gaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt        >  2:383468/1‑150 (MQ=255)
                                                                                                                                        gaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt        <  2:42308/150‑1 (MQ=255)
                                                                                                                                         aaaaaaGGCCGCAGCGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGATTATCCTCGCCCGTTTTCGGGTTa       >  1:533716/1‑150 (MQ=255)
                                                                                                                                         aaaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGGGCCGTGCTGTAATGGGAATATGCTCGCCCGTTTTCGGGGTa       >  1:1383819/1‑150 (MQ=255)
                                                                                                                                         aaaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTa       >  2:1775500/1‑150 (MQ=255)
                                                                                                                                          aaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGCATCGTTTTCGACCCGGCTTGTTAACTTCTGCCCGGGTGTGAAGGTCACCACGCGCCGTGCGGTATTGGGTATCTCGTCGCCGGTGTTCGGGttgc      >  1:299641/1‑148 (MQ=255)
                                                                                                                                            aaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGACCGGGTCTGAAGGTCACCACTCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGt    >  1:314209/1‑150 (MQ=255)
                                                                                                                                              aGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCACGTTTTCGGGTTACGTcc  >  1:1253063/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCC  >  NC_000913/1795078‑1795369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: