Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,807,318 A→G 7.4% intergenic (+19/‑82) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,3180AG 7.4% 313.7 / 12.5 108intergenic (+19/‑82)pfkB/ydiZ6‑phosphofructokinase II/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (54/46);  new base G (3/5);  total (57/51)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

Only 100 of 109 total aligned reads displayed.

AGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAAT  >  NC_000913/1807170‑1807467
                                                                                                                                                    |                                                                                                                                                     
aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAt                                                                                                                                                      >  2:513589/1‑150 (MQ=255)
         ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAat                                                                                                                                             <  1:857634/150‑1 (MQ=255)
          tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatg                                                                                                                                            <  2:1636971/150‑2 (MQ=255)
          tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGAGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatg                                                                                                                                            <  1:558362/150‑2 (MQ=255)
           ctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgt                                                                                                                                           <  1:490364/150‑3 (MQ=255)
              ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgtttt                                                                                                                                        <  2:479684/150‑6 (MQ=255)
               tcaaGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCGGTGCTCCCATGACGATACGCTAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAGTGGGGGAatcatccc                                                                                                                                       >  2:170106/3‑148 (MQ=255)
               tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAc                                                                                                                                       <  1:1298259/150‑1 (MQ=255)
               agAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGACTGTGCTACCAAGACGATACGCAAAAAATTTACGCTTAACTTTCACGATAACAAAAACATTCCCCCAGCAAAGGGGGAATCATCAc                                                                                                                                       >  1:320540/2‑150 (MQ=255)
                gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAcc                                                                                                                                      >  2:1459593/1‑150 (MQ=255)
                    agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  2:1646658/150‑1 (MQ=255)
                    agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  1:1676980/150‑1 (MQ=255)
                        ggTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                                                                                                                              >  1:943575/1‑150 (MQ=255)
                         gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCg                                                                                                                             >  1:782415/1‑150 (MQ=255)
                           tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGc                                                                                                                           <  1:1328864/150‑1 (MQ=255)
                           tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGc                                                                                                                           <  2:638506/150‑1 (MQ=255)
                             gTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACCCGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCaa                                                                                                                         >  1:1420934/1‑150 (MQ=255)
                                ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                      <  2:1359995/150‑1 (MQ=255)
                                 tGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCGACCTGTCGGCAAcgcg                                                                                                                     >  1:1409339/1‑150 (MQ=255)
                                     gTAGCTGCGGGGAGTGCAGCCACACTCTATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtc                                                                                                                 <  2:318745/150‑1 (MQ=255)
                                       aGCTGCGGGGAGTGCCGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTACCAAAAACATTCCCCCCGCATTGGGGGATTCATCACCATCCTGTCGGCAGCGCGTTtctc                                                                                                               >  1:908400/1‑150 (MQ=255)
                                         ctgctgGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                             <  2:71502/150‑1 (MQ=255)
                                         cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                             <  2:1006276/150‑1 (MQ=255)
                                          tGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                                                                                                            >  1:28418/1‑150 (MQ=255)
                                           gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGAc                                                                                                           <  1:1279643/150‑1 (MQ=255)
                                            cGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACt                                                                                                          <  2:1526289/150‑1 (MQ=255)
                                             ggggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTa                                                                                                         >  2:1272045/1‑150 (MQ=255)
                                             ggggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTa                                                                                                         <  2:164135/150‑1 (MQ=255)
                                                  gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                    >  1:329668/1‑150 (MQ=255)
                                                  gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                    >  1:41651/1‑150 (MQ=255)
                                                   tGCAGCCAAACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCATACCTTTCCCGCTACCAAAAACATTCCCCCAGAATTGGGGGAATCATAACCAACATGTCTGCAAAGCGTTTCTCCGACTATGCTCa                                                                                                   >  1:1225764/1‑150 (MQ=255)
                                                    gCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaa                                                                                                  >  2:1019304/1‑150 (MQ=255)
                                                       gCCCCACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCGCCGACTATGCTCAAAAg                                                                                               >  1:922812/1‑150 (MQ=255)
                                                       gCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGACTCATCACCCACCTGTCGGCACCGCGTTTCTCCGGCTAGGCGCAAAAg                                                                                               >  1:922807/1‑150 (MQ=255)
                                                       gCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAg                                                                                               >  2:1520342/1‑150 (MQ=255)
                                                         cacaCTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGATCAAAAGTc                                                                                             >  2:187519/1‑150 (MQ=255)
                                                            acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtg                                                                                          <  1:19034/150‑1 (MQ=255)
                                                            acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtg                                                                                          <  1:1121042/150‑1 (MQ=255)
                                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         >  1:1077488/1‑150 (MQ=255)
                                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         >  1:709177/1‑150 (MQ=255)
                                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         >  1:1021587/1‑150 (MQ=255)
                                                              tCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgtg                                                                                        <  1:465138/150‑1 (MQ=255)
                                                               cAATCAGGGAACACGTCTCTGCTCCCATGACGAAACGCAAAAAATTTAAGCTTAAATTTAACGATAACAAAAACATACCCCCAGAATTTGGGGAATCATAACCAACCTGTCCGCAACGCCTTTCACCGACTATGCTCAAAAGTCAtgtta                                                                                       >  2:1539819/1‑148 (MQ=255)
                                                                      ggAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACaaa                                                                                >  2:1027130/1‑150 (MQ=255)
                                                                       gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACAAACCTGACCGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAg                                                                               >  1:285778/1‑150 (MQ=255)
                                                                        aaCACGTCTGTGCTCCGATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgg                                                                              <  2:187399/150‑1 (MQ=255)
                                                                        aaCACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgg                                                                              >  1:1715238/1‑150 (MQ=255)
                                                                        aaCACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgg                                                                              <  1:1624318/150‑1 (MQ=255)
                                                                          cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgggg                                                                            <  2:794238/150‑1 (MQ=255)
                                                                            cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                          >  1:1063192/1‑150 (MQ=255)
                                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGa                                                                         <  2:504220/150‑1 (MQ=255)
                                                                               cTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAAc                                                                       <  2:1033207/150‑1 (MQ=255)
                                                                                 gtgCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTa                                                                     <  2:1640446/150‑1 (MQ=255)
                                                                                 gtgCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTa                                                                     <  2:391580/150‑1 (MQ=255)
                                                                                   gCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                                   >  1:706344/1‑150 (MQ=255)
                                                                                   gCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                                   >  1:500135/1‑150 (MQ=255)
                                                                                    cTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATgg                                                                  >  1:1598062/1‑150 (MQ=255)
                                                                                     tCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGc                                                                 >  1:760163/1‑150 (MQ=255)
                                                                                      cccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGcc                                                                >  2:463860/1‑150 (MQ=255)
                                                                                      cccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGAAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGcc                                                                >  2:284038/1‑150 (MQ=255)
                                                                                        cATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGTGGGAACTATGGCCAg                                                              <  1:78800/150‑1 (MQ=255)
                                                                                           gACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATACCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGTTGAACTATGGCCAGTgg                                                           >  2:1795360/1‑150 (MQ=255)
                                                                                               aTACGCAGAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                                                       >  2:1546573/1‑150 (MQ=255)
                                                                                                 aCGCAAAAAATTTACGCATACCATTCCCGCAAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTATCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCt                                                     <  1:342272/150‑1 (MQ=255)
                                                                                                    cAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGt                                                  >  1:329265/1‑150 (MQ=255)
                                                                                                       aaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCg                                               <  2:706344/150‑1 (MQ=255)
                                                                                                       aaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCg                                               <  2:943575/150‑1 (MQ=255)
                                                                                                          aTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTa                                            <  1:380760/150‑1 (MQ=255)
                                                                                                           tttACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTAt                                           <  2:782415/150‑1 (MQ=255)
                                                                                                               cccTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCCCCAACCTGTCGGCAACGCGTTTCTCCTACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCa                                       <  2:1645332/148‑1 (MQ=255)
                                                                                                               cGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCa                                       >  2:1252935/1‑150 (MQ=255)
                                                                                                               cGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCa                                       <  1:1520342/150‑1 (MQ=255)
                                                                                                                gCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAt                                      >  2:1714245/1‑150 (MQ=255)
                                                                                                                gCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAt                                      >  1:1731177/1‑150 (MQ=255)
                                                                                                                gCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAt                                      <  1:1038002/150‑1 (MQ=255)
                                                                                                                 cTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCACCCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGACCTATGGCCAGTGGCGATCTTGTCCGTTATGTCATa                                     >  1:333725/1‑150 (MQ=255)
                                                                                                                     ccTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCg                                 <  1:1459593/150‑1 (MQ=255)
                                                                                                                     ccTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCATTATGTCATAACCg                                 <  2:28418/150‑1 (MQ=255)
                                                                                                                       tttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTc                               >  1:1301419/1‑150 (MQ=255)
                                                                                                                            cGCTAACAAAAACATTCACCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTACGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCGAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGtt                          >  1:514741/1‑150 (MQ=255)
                                                                                                                             gCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTg                         >  2:900716/1‑150 (MQ=255)
                                                                                                                                 aCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATg                     <  2:1650432/150‑1 (MQ=255)
                                                                                                                                 aCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATg                     <  2:1409339/150‑1 (MQ=255)
                                                                                                                                  cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAATGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  2:1450453/1‑150 (MQ=255)
                                                                                                                                  cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  1:1017525/1‑150 (MQ=255)
                                                                                                                                  cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAACTGTCGGCAACGAGTATCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  1:274500/1‑150 (MQ=255)
                                                                                                                                   aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                   <  1:1027130/150‑1 (MQ=255)
                                                                                                                                    aaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAgg                  >  2:687618/1‑150 (MQ=255)
                                                                                                                                      aaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCGGACTATGCTCAATAGTCATGTGATAACAAAGGGGTGATCTATGGCCAGTGGCGATCTTGTCCGTTATGACATAACCGTCATGTTGCATGAGGAt                <  2:1678708/150‑1 (MQ=255)
                                                                                                                                       tgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCTGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATa               >  2:778005/3‑150 (MQ=255)
                                                                                                                                        gaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATAc              >  1:1042003/2‑150 (MQ=255)
                                                                                                                                         aTTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGGCATAACCGTCATGTTGCATGAGGATACa             <  2:168263/150‑1 (MQ=255)
                                                                                                                                            cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg          <  1:45170/150‑1 (MQ=255)
                                                                                                                                            cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg          >  2:801446/1‑150 (MQ=255)
                                                                                                                                              cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTTAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc        <  1:602024/150‑1 (MQ=255)
                                                                                                                                              cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc        >  2:839626/1‑150 (MQ=255)
                                                                                                                                              cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc        <  2:213994/150‑1 (MQ=255)
                                                                                                                                               ccAGCATTGTGGGAATGATCACCAACCTGTCGTCAACGCGTTTCTCCGTCTATGCTCAAAACTCATGTGATAACAAAGGTGTGATCTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATTATGATACATTGACt       <  2:1225764/150‑1 (MQ=255)
                                                                                                                                               ccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACt       <  1:513589/150‑1 (MQ=255)
                                                                                                                                                    aTTGGTGTAATCATCACCAACGTGTCGGCAACGCGTTTATCCTACTATGCTCAAAAGTCATGTTATAACAAAGGGGTTAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCTTTACCGTCATGTTGCATGAGGATACATTGACTGAAAt  <  1:284038/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAAT  >  NC_000913/1807170‑1807467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: