Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,822,073 T→C 9.5% I62V (ATC→GTC) ‡ osmE ← osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0730TC 9.5% 279.9 / 18.1 106I62V (ATC→GTC) ‡osmEosmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (49/45);  new base C (5/5);  total (54/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 108 total aligned reads displayed.

CGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCG  >  NC_000913/1821924‑1822222
                                                                                                                                                     |                                                                                                                                                     
cGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGAGAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt                                                                                                                                                       <  2:1210302/150‑1 (MQ=255)
  cAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                                                                                                                     >  2:1389384/1‑150 (MQ=255)
  cAGCCTGTGGAGCAGTGTCGTATTCAGGACAGGTCTGATAACCTGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCCCGTAGAGCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                                                                                                                     <  1:882956/150‑1 (MQ=255)
   aGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACAATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCAt                                                                                                                                                    >  1:1670230/1‑150 (MQ=255)
    gCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCAGGGATCagg                                                                                                                                                   >  1:918777/1‑148 (MQ=255)
     ccTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                                                                                                                  >  2:854703/1‑150 (MQ=255)
     ccTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                                                                                                                  <  1:1512473/150‑1 (MQ=255)
     ccTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                                                                                                                  <  1:589020/150‑1 (MQ=255)
        ttgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcg                                                                                                                                               <  2:505696/149‑2 (MQ=255)
        gtgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGAAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCa                                                                                                                                               >  2:325507/1‑150 (MQ=255)
         tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                                                                                                              <  1:1733052/150‑1 (MQ=255)
         tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgc                                                                                                                                              <  2:13295/150‑3 (MQ=255)
          ggATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                                                                                                             >  2:1352953/1‑150 (MQ=255)
           gATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGGCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgcgg                                                                                                                                            <  1:45363/150‑5 (MQ=255)
           gATCAGTGTCGTAATCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgcgg                                                                                                                                            <  1:467331/150‑5 (MQ=255)
             tCAGTGTCGTTTTCAGCACAGGTCTGATAAGCGGAGTTGATGACATGTCCGGTATCTTCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                                                                                                          <  1:5933/150‑1 (MQ=255)
              cAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTATCGCGACAAAGTAGGTTTCTGCTTTAACATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAg                                                                                                                                         <  2:1658859/150‑1 (MQ=255)
                  gtCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGa                                                                                                                                     >  2:635853/1‑150 (MQ=255)
                        ttCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACTAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt                                                                                                                               >  2:1191376/1‑150 (MQ=255)
                        ttCAGCACAGGTCTGATAACCGGAGTGGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTTCCATCACGGTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt                                                                                                                               <  2:1764607/150‑1 (MQ=255)
                           aGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                                                                                                            <  1:1810175/150‑1 (MQ=255)
                            gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAccc                                                                                                                           <  1:1612577/150‑1 (MQ=255)
                              acaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGc                                                                                                                         >  2:833971/1‑150 (MQ=255)
                                 ggTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCaaa                                                                                                                      >  1:143066/1‑149 (MQ=255)
                                    cTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtg                                                                                                                   >  2:595331/1‑150 (MQ=255)
                                      gATAACCGGAGTTGATGACATGTCCGGTATTATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCTCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                                                                                                                 >  1:1298162/1‑150 (MQ=255)
                                      gATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                                                                                                                 <  2:758582/150‑1 (MQ=255)
                                       aTAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGc                                                                                                                <  1:45460/150‑1 (MQ=255)
                                           ccGGAGTTGATGACATGTCCGGTATCATCAAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGAAAGTACCGCGAGCAAGGATCATGCTCACTTCAGACGAACGTTTACCCGCAATTTGAGCAAcc                                                                                                            >  1:955063/1‑150 (MQ=255)
                                                gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcgc                                                                                                       >  1:72359/1‑150 (MQ=255)
                                                     tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGc                                                                                                  <  1:165405/150‑1 (MQ=255)
                                                     tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATAACGTTGACCCAGGATGTACGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGc                                                                                                  >  1:718683/1‑150 (MQ=255)
                                                       aCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc                                                                                                <  1:1076991/150‑1 (MQ=255)
                                                         aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt                                                                                              <  1:1572488/150‑1 (MQ=255)
                                                            tCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGcc                                                                                           >  1:1303959/1‑150 (MQ=255)
                                                               ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttt                                                                                        >  2:716278/1‑150 (MQ=255)
                                                                gTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttt                                                                                       >  1:17341/1‑150 (MQ=255)
                                                                 tATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttttt                                                                                      <  2:830957/150‑1 (MQ=255)
                                                                  atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttttt                                                                                     <  1:1250517/150‑1 (MQ=255)
                                                                  atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttttt                                                                                     >  2:674658/1‑150 (MQ=255)
                                                                  atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTCCCCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttttt                                                                                     >  1:1632737/1‑150 (MQ=255)
                                                                    catcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGa                                                                                   <  1:325507/150‑1 (MQ=255)
                                                                      tcTAACGCGACAAAGTAGGATTCTGCTATACCATCACGTTGACCCCGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACg                                                                                 <  1:1119662/150‑1 (MQ=255)
                                                                       cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGt                                                                                >  1:892656/1‑150 (MQ=255)
                                                                        tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTc                                                                               <  2:764599/150‑1 (MQ=255)
                                                                         aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCt                                                                              >  1:1220485/1‑150 (MQ=255)
                                                                           cgcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCttt                                                                            >  1:595455/1‑150 (MQ=255)
                                                                           cgcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCttt                                                                            >  1:809277/1‑150 (MQ=255)
                                                                            gcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTc                                                                           <  2:1047229/150‑1 (MQ=255)
                                                                                 aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCTCAAATTATTCAACCTGCGCCCGGCTCATGCCTATTTTGACGTCTTTcaccaa                                                                      >  2:266568/1‑149 (MQ=255)
                                                                                     tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGc                                                                  <  1:716278/150‑1 (MQ=255)
                                                                                      cggTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGca                                                                 <  2:1352434/149‑2 (MQ=255)
                                                                                         tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGCAGGTTTACCCGCAATTTGTGCCACCGGCGCCCGGCTCATGCCTTTTTTGCCGTCTTTCACCACAGGCGGTa                                                              >  1:1824185/1‑150 (MQ=255)
                                                                                         tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTa                                                              <  1:1157923/150‑1 (MQ=255)
                                                                                                 ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTgg                                                      <  2:1756066/150‑1 (MQ=255)
                                                                                                  tACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGt                                                     <  2:479773/150‑1 (MQ=255)
                                                                                                        ctcGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGTCGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTGTTTGACGTCTTTCAGCACAGGCTGTACAATCTGGTCTTTgg                                               <  2:384105/148‑1 (MQ=255)
                                                                                                        cACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCTCTTCAGACGAAGGTTTACCCGCATTTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTgg                                               <  2:1670230/150‑1 (MQ=255)
                                                                                                         aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGt                                              >  2:484807/1‑150 (MQ=255)
                                                                                                          cGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCGTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTa                                             >  1:1180182/1‑150 (MQ=255)
                                                                                                           gTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTAc                                            <  2:1262072/150‑1 (MQ=255)
                                                                                                               aCCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATc                                        >  2:208561/1‑150 (MQ=255)
                                                                                                                cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCa                                       >  1:1352166/1‑150 (MQ=255)
                                                                                                                cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCa                                       >  1:600753/1‑150 (MQ=255)
                                                                                                                cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCa                                       <  1:531215/150‑1 (MQ=255)
                                                                                                                cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGAGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCa                                       >  1:1160024/1‑150 (MQ=255)
                                                                                                                cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTATACCCGCAATTTGTGCATCCTGCGCCCGGCTCAAGCCTTTTTTGACTTCTTTCACCACAGGCTGTACATACTGGTCTGTGGTTCGATCa                                       >  1:286810/1‑150 (MQ=255)
                                                                                                                cccAGGATGTAGGGCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGTTTTACCCGCAATTTCTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCa                                       <  1:292516/150‑1 (MQ=255)
                                                                                                                  cAGGATGAAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACATCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATa                                     >  2:225136/1‑150 (MQ=255)
                                                                                                                    ggATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAg                                   <  2:454400/150‑1 (MQ=255)
                                                                                                                     gATGGAGGTCTGGCAAGGACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCGCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGc                                  <  1:1593795/150‑1 (MQ=255)
                                                                                                                      aTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGcc                                 >  2:1435319/1‑150 (MQ=255)
                                                                                                                       tGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCg                                <  2:809277/150‑1 (MQ=255)
                                                                                                                        gTAGGTCTGGCTAGTACCGCGAGCATTGATCATGCTCACTTCAGACGATGGTTTTGCCGCAATTTGTGCAACCTGCTCCCGGCTGATGCCTTTTTTTTCGTCTTTCTCCACTGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGt                               <  1:1638741/150‑1 (MQ=255)
                                                                                                                         tAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTa                              <  1:595331/150‑1 (MQ=255)
                                                                                                                           ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACa                            >  2:1624803/1‑150 (MQ=255)
                                                                                                                            gTCTGGCAAGTACCGCGAGCATGGATCAAGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCCCAGGCTGTACACACTGCTCTTTGTTACGATCATAAGCCGTActc                           >  2:1798601/1‑148 (MQ=255)
                                                                                                                              cTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAAcc                         <  2:163818/150‑1 (MQ=255)
                                                                                                                              cTGGCAAGGACCGCGAGCATGGAGCATTCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCGCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAAct                         <  2:277720/150‑2 (MQ=255)
                                                                                                                                 gCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGc                      >  2:1071496/1‑150 (MQ=255)
                                                                                                                                  cAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGcc                     <  2:1144039/150‑1 (MQ=255)
                                                                                                                                   cacgaCCGCGAGCATGGAGACAGCGCACTTCAGACGAAGTTTTCCCCGCAATTTCGGCACCCTGCGCCAGGCTCATGCCTTCTTTCACGTCTATCACCACAGGCTGTACAACCTGGTCTTTGGTACGATCATAATCCGTACAACCCGCCa                    <  2:1173845/146‑1 (MQ=255)
                                                                                                                                   aaGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCa                    <  1:854703/150‑1 (MQ=255)
                                                                                                                                    aGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAg                   >  2:1676443/1‑150 (MQ=255)
                                                                                                                                    aGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCAGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAg                   >  1:907931/1‑150 (MQ=255)
                                                                                                                                     ctgaagtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGc                  >  1:1570657/8‑150 (MQ=255)
                                                                                                                                      tgaagtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCa                 >  1:155398/7‑150 (MQ=255)
                                                                                                                                      tACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCa                 <  2:72359/150‑1 (MQ=255)
                                                                                                                                        ccGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATg               >  1:1175683/1‑150 (MQ=255)
                                                                                                                                        ccGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATg               >  2:1553650/1‑150 (MQ=255)
                                                                                                                                          gtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCAGCCAGCATGGt             >  1:1349183/3‑150 (MQ=255)
                                                                                                                                          gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt             <  1:1191376/150‑1 (MQ=255)
                                                                                                                                          gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt             >  1:1499833/1‑150 (MQ=255)
                                                                                                                                          gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt             >  1:248967/1‑150 (MQ=255)
                                                                                                                                          gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCGTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt             >  1:684777/1‑150 (MQ=255)
                                                                                                                                           cgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGtt            <  2:1542440/150‑1 (MQ=255)
                                                                                                                                            gAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGAGCCCGGATCATGCCTTTTTTGACGTCTTTCACCAGAGTCTGTTCATAGTGGTCTTTGGTCCGATCATAAGCCGTACAACGCGCCAGCTAGGTTa           >  2:748792/1‑150 (MQ=255)
                                                                                                                                              gCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAAt         <  1:547109/150‑1 (MQ=255)
                                                                                                                                                 tGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATAcc      >  1:305148/1‑150 (MQ=255)
                                                                                                                                                     tCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATAccgccg  <  1:1802990/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCG  >  NC_000913/1821924‑1822222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: