Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 5.6% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 5.6% 321.3 / 4.8 108intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (56/44);  new base A (2/4);  total (59/49)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 115 total aligned reads displayed.

GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907
                                                                                                                                                     |                                                                                                                                                     
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                     >  2:312962/1‑150 (MQ=255)
   atGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTTACCAAAGAGAAACCTGCACATCTGGCGCAATCAATTCGCGATGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGCTGc                                                                                                                                                    >  1:238510/1‑150 (MQ=255)
   atGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGc                                                                                                                                                    <  1:1646813/150‑1 (MQ=255)
     gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                  >  1:803920/1‑150 (MQ=255)
     gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                  >  1:19056/1‑150 (MQ=255)
       cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                <  1:901685/150‑1 (MQ=255)
            gatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATcc                                                                                                                                           >  2:1486284/1‑150 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                        <  1:940702/150‑1 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                                                                        <  1:262570/150‑1 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                                                                        <  1:241164/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  2:971129/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       >  1:536515/1‑150 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATACATCCGGca                                                                                                                                       >  1:1722021/1‑150 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:1794233/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:1672526/150‑1 (MQ=255)
                   aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                    <  2:520135/150‑1 (MQ=255)
                   aCAAAACATTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                   <  1:491417/150‑1 (MQ=255)
                    cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                   >  1:1164986/1‑150 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 >  2:1393150/1‑150 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 >  2:21776/1‑150 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACacaac                                                                                                                                 <  1:873767/150‑1 (MQ=255)
                         cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAtt                                                                                                                              <  1:241765/150‑2 (MQ=255)
                              gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACTTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                         >  1:317103/1‑150 (MQ=255)
                              gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCAATCGCGTGGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                         <  1:1015404/150‑1 (MQ=255)
                                 tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                      >  2:929864/1‑150 (MQ=255)
                                 tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATTCCCGTTGCGAAACGTTAAATTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                      <  1:157190/150‑1 (MQ=255)
                                  gtgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                                                                     >  2:1255289/1‑150 (MQ=255)
                                   tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc                                                                                                                    >  2:1606693/1‑150 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATTCATCCGGCACACAACAACACACCATATcc                                                                                                                   >  2:919488/1‑150 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAAATCAAACGGCCCACAACATCACACCatatac                                                                                                                   >  1:1386288/1‑148 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                   <  1:1288703/150‑1 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                   <  1:1266637/150‑1 (MQ=255)
                                      aGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGCCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAg                                                                                                                 >  2:226901/1‑150 (MQ=255)
                                      aGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAg                                                                                                                 >  2:1223602/1‑150 (MQ=255)
                                      aGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACCCCACATCACACCATATACac                                                                                                                 >  1:1688204/1‑149 (MQ=255)
                                       gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                >  1:486560/1‑150 (MQ=255)
                                        aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                               >  2:1685593/1‑150 (MQ=255)
                                           cGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCAGCGCAg                                                                                                            >  2:1733899/1‑150 (MQ=255)
                                            ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCTCAATACATTGGGGTTGGGAAACGTTATTTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGt                                                                                                           <  2:1742945/150‑1 (MQ=255)
                                                  cGCTTCAGAAACGCAAAAAGTGACCACAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  2:1252915/150‑1 (MQ=255)
                                                  cGCTTCAGAAACGAAAAATGTTACCAAATAGAAACCTGCACATCTGGCGCAATCAATTCGCGTTGCTAAACTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  1:466185/150‑1 (MQ=255)
                                                  cGCTTCACAAACGCAAAATGTGACCAAAGAGAACCGTGCACTTCGGGCGCAATCCATTCGCGTTGCTAAACTTTAATTTACGTTAATTTGGTGTGCCGGGTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  2:1756196/150‑1 (MQ=255)
                                                   gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCTATCCATTCGCCTTGAGAAACGTTAATTTACGTTAATGTTGTGACCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACCAGCCCAGTTTTTcca                                                                                                    >  1:221210/1‑149 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   >  1:226055/1‑150 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCAACCCGCCCACAACCTAACACCATATACAGCGCACTTTTTACtattt                                                                                                >  1:1640155/1‑149 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                <  1:31493/150‑1 (MQ=255)
                                                        aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgg                                                                                               <  2:879742/150‑1 (MQ=255)
                                                         gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              <  2:1239904/150‑1 (MQ=255)
                                                         gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              >  2:829293/1‑150 (MQ=255)
                                                           aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                            >  2:255120/1‑150 (MQ=255)
                                                             cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCCCAACATCCCCCCCTATACCGCGCAGTTTTTCGTTTTGGTGCCg                                                                                          >  2:465621/1‑150 (MQ=255)
                                                             cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                          >  2:155349/1‑150 (MQ=255)
                                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCAGGCACACAAAATCACACCATATCCAGCGGAGTTTTTCCATTTGGTGCCgg                                                                                         >  1:1345341/1‑150 (MQ=255)
                                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATCCATCCGGCAGACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                         >  2:1724287/1‑150 (MQ=255)
                                                               cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCAAGAGAAGTTTTTCCTTTTGGTGCCGGa                                                                                        >  1:156002/1‑150 (MQ=255)
                                                               cAAAAAGAGCCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGATGCGAAACGTTAATATACGTTAATGTTGTGTGCCGGGTTCAAAGCAACCCGCACACAACATAACACCATAACCACCCCAGTTTTTACTTTTGCTGCCCGa                                                                                        >  1:378131/1‑150 (MQ=255)
                                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGCCGCAATCCATTCTCGTTGCGAAACGTTAATTTACGTTAATGGTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                       <  1:121256/150‑1 (MQ=255)
                                                                    aGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                   >  2:40154/1‑150 (MQ=255)
                                                                        aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATACGCGAGGCGAAACGTTAATTTAGGTAAATCTTGTGTGCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                               <  2:164979/150‑1 (MQ=255)
                                                                         ccAAAGAGAAACCTGCACATCTTGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCAGCGGAGTTTTTGATTTTGGTGCCGGATATGCCTTAt                                                                              >  1:113080/1‑150 (MQ=255)
                                                                           aaaGAGATACCTGCACATCTGGCGCAATCCATTGGCGTTGCGAATCGTTAATTTTCGTTAATGTTGCGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTATGCTTTTGGTGCCGGATATGCCTTATcc                                                                            >  1:1033821/1‑150 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            <  2:174812/150‑1 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTAACGTAAATGTTTGGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            <  1:1607376/150‑1 (MQ=255)
                                                                               agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                        <  2:1227617/150‑1 (MQ=255)
                                                                               agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAATTCATCCGGCACACAACATAACCCCATATACAGCGCAGTTTTTACTTTTGGTGCCGGATATTCCTTTTACAGCa                                                                        >  2:1347572/1‑150 (MQ=255)
                                                                                   aCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                                                                    <  1:616286/150‑1 (MQ=255)
                                                                                    ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                   >  1:1448728/1‑150 (MQ=255)
                                                                                    ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                   <  2:1598400/150‑1 (MQ=255)
                                                                                    ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCTTATACAGCATAGCt                                                                   >  2:1826506/1‑150 (MQ=255)
                                                                                       gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCCGGTGCAATGCAACCGGCACACAACATCACACCAAATACCACGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                >  2:556223/1‑150 (MQ=255)
                                                                                            cctgccccAAGCCATCCCCGGTGCGAAACGGAAATTTACGTAAATCGGTCGTGCCCGGTCCAAGGCAACCGGCACACAACATCACACCATATCCACCGCAGTTTTTCCATATGCTGCCGGATAAGCCTTATCCAGCATAGCTAATACCGc                                                           <  2:683603/143‑1 (MQ=255)
                                                                                             gtGGCGCAATCCATTCGCGTTGCGAAACTTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGAATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          <  1:1663265/149‑1 (MQ=255)
                                                                                              tGGCGAAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                         <  1:360695/150‑1 (MQ=255)
                                                                                               ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCAGGGTGCAATGCATCCGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCgagga                                                        >  2:1739839/1‑146 (MQ=255)
                                                                                                gctcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                       <  1:890781/147‑1 (MQ=255)
                                                                                                gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                       >  2:1140324/1‑150 (MQ=255)
                                                                                                gcgaAATCCATTCGCGTTGCTAACCTTTATTTTACGTAATTGTGGTGGGCCGGGTCAAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                       <  1:1370672/150‑1 (MQ=255)
                                                                                                 cgaaaTCCATTCGGGTGGCGAAAGGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                      <  2:803920/147‑1 (MQ=255)
                                                                                                  gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTTGTGCCGGATATGCCTTATACAGCATAGCTAATTCCGCTgaaga                                                     >  2:1662915/1‑150 (MQ=255)
                                                                                                    aaTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                   >  2:1682370/1‑150 (MQ=255)
                                                                                                        cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAAAGCAAACGGCACACAACAAAACAACAAATACACCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAAATACGCTGAAGCAAGTTc                                               >  2:1318359/1‑150 (MQ=255)
                                                                                                         aTTCGCGTTGCTAAACTTTAATTTAGTTTATTGTTGTGTGCCGGTTTCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                              <  2:1459081/150‑1 (MQ=255)
                                                                                                          ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                             <  1:34360/150‑1 (MQ=255)
                                                                                                           tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAc                                            <  2:34327/150‑1 (MQ=255)
                                                                                                               gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                        >  1:1783087/1‑150 (MQ=255)
                                                                                                                       tggTTAATTAACGTTATTGTTGTGTGCCGGGTGAAATTCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGc                                <  1:919488/148‑1 (MQ=255)
                                                                                                                        cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                               >  1:698013/1‑150 (MQ=255)
                                                                                                                                    ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                   <  1:117351/150‑1 (MQ=255)
                                                                                                                                           tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATggg            >  2:149571/1‑150 (MQ=255)
                                                                                                                                           tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg            <  1:963214/150‑1 (MQ=255)
                                                                                                                                           tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg            <  1:477308/150‑1 (MQ=255)
                                                                                                                                              gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  1:1046417/1‑150 (MQ=255)
                                                                                                                                              gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  2:130765/1‑150 (MQ=255)
                                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  2:65300/1‑150 (MQ=255)
                                                                                                                                                   gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg    <  2:19056/150‑1 (MQ=255)
                                                                                                                                                    ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg   >  1:1339741/1‑150 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  2:465906/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  1:1682370/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  1:21776/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  1:1347572/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: