Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,916 C→T 5.1% intergenic (‑170/+35) fadD ← / ← yeaY acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,9160CT 5.1% 375.3 / 4.8 116intergenic (‑170/+35)fadD/yeaYacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base C (45/65);  new base T (2/4);  total (47/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889767‑1890062
                                                                                                                                                     |                                                                                                                                                  
tgtTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAc                                                                                                                                                    >  1:983193/1‑150 (MQ=255)
tgtTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAc                                                                                                                                                    >  2:1291416/1‑150 (MQ=255)
 gtTCGGCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAcc                                                                                                                                                   <  2:76648/150‑1 (MQ=255)
     gtcgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGcc                                                                                                                                               <  2:1046196/150‑1 (MQ=255)
       cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                             >  1:1763683/1‑150 (MQ=255)
        gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            >  1:1524426/1‑150 (MQ=255)
        gtaaTCTCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGATTTATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            <  1:160147/147‑1 (MQ=255)
             cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGCAACAGCGGCTGGTCCGCTGtttc                                                                                                                                       <  1:1437763/150‑1 (MQ=255)
             cGCAACCCCAAATGATATACAAGCCGTTAACATAATATTAACTCATCATACCAGCTTGCTAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttc                                                                                                                                       <  2:902329/150‑1 (MQ=255)
              gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttct                                                                                                                                      <  1:438713/150‑1 (MQ=255)
              gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttct                                                                                                                                      >  2:1756766/1‑150 (MQ=255)
               cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctt                                                                                                                                     >  1:1161682/1‑150 (MQ=255)
                aaCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctgc                                                                                                                                    <  2:1493776/150‑3 (MQ=255)
                 aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  2:1311598/1‑150 (MQ=255)
                 aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  1:1774150/1‑150 (MQ=255)
                  ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                  <  1:369550/150‑1 (MQ=255)
                  ccccAAATGATATACATGCAGTTAACATAATATAAACTCATCATACCAGCTTGATAATTACCCAACTTAAAGGGTGCGAAGCGCGTCACTATTTATTTTAATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCTGTGtttcttttt                                                                                                                                  <  1:816197/150‑1 (MQ=255)
                   cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1790321/150‑1 (MQ=255)
                   cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  2:855569/150‑1 (MQ=255)
                   cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1049995/150‑1 (MQ=255)
                     cAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttctt                                                                                                                               >  2:1251119/1‑150 (MQ=255)
                      aaaTGATATACATGCCGTTAACATTATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  2:277518/150‑1 (MQ=255)
                      aaaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  1:524478/150‑1 (MQ=255)
                       aaTTATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1234389/150‑1 (MQ=255)
                       aaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1725251/150‑1 (MQ=255)
                        aTGATATACATGCAGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaa                                                                                                                            <  1:251054/150‑1 (MQ=255)
                         tGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaa                                                                                                                           <  2:983193/150‑1 (MQ=255)
                         tGAAATACATGCCGTAAACATAATATTAACTCATCATACCAGCTTGAAAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTAAACCCTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaa                                                                                                                           <  1:1210884/150‑1 (MQ=255)
                              taCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACaa                                                                                                                      >  2:250823/1‑150 (MQ=255)
                                 aTGCCTTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGGCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCa                                                                                                                   <  1:1219330/150‑1 (MQ=255)
                                   tcagTTAACATAATATTAACTAATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCTAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAat                                                                                                                 <  2:1149062/147‑1 (MQ=255)
                                          aCATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAg                                                                                                          <  1:771163/150‑1 (MQ=255)
                                           cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGt                                                                                                         >  1:401541/1‑150 (MQ=255)
                                            ataataTTAACTCATCATTCCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAAGTATTCAGtt                                                                                                        >  2:149267/1‑150 (MQ=255)
                                            ataataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGtt                                                                                                        >  2:438927/1‑150 (MQ=255)
                                             taataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTa                                                                                                       <  2:1175713/150‑1 (MQ=255)
                                                tatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACaa                                                                                                    >  1:665117/1‑150 (MQ=255)
                                                     aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                               >  1:395352/1‑150 (MQ=255)
                                                     aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                               >  1:597821/1‑150 (MQ=255)
                                                      cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:1119713/150‑1 (MQ=255)
                                                      cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  2:1761310/150‑1 (MQ=255)
                                                      cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:981660/150‑1 (MQ=255)
                                                        catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCCGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGt                                                                                            >  2:61034/1‑150 (MQ=255)
                                                         atcatACCAGCTTGATAATTAACCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTa                                                                                           <  1:252613/150‑1 (MQ=255)
                                                            atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCTCGTCACAATATAAATTTATCTTAACCGTAAGAATTCAGAAACACCGGACCAGCCGCTGATTCTTTATATGTAAAAACAAGCAAATATACAGTTAAAAAAGTTTGTtctc                                                                                        >  1:1267641/1‑150 (MQ=255)
                                                            atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTActc                                                                                        >  2:1454376/1‑150 (MQ=255)
                                                               ccAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcg                                                                                     <  1:1290168/150‑1 (MQ=255)
                                                                   cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 >  1:1570306/1‑150 (MQ=255)
                                                                   cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 <  2:661986/150‑1 (MQ=255)
                                                                     tGATAATTACCCAACGAAACGGTTGCGAAGCGCTGCACTATTTATTTTTATCTTTCCGGTATGAATGGAGACACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                               <  2:963068/150‑1 (MQ=255)
                                                                       aTAATTACCCAACGAAAAGTTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCg                                                                             <  2:183074/150‑1 (MQ=255)
                                                                         aaTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                           <  2:610917/150‑1 (MQ=255)
                                                                          aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                          <  2:14024/150‑1 (MQ=255)
                                                                           ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                         <  1:586411/150‑1 (MQ=255)
                                                                           ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                         <  2:948526/150‑1 (MQ=255)
                                                                            tACCCAACGAACAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTCAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  2:272951/150‑1 (MQ=255)
                                                                            tACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  1:618851/150‑1 (MQ=255)
                                                                             aCCCAACGAAATGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                       <  2:1084361/150‑1 (MQ=255)
                                                                             aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCAGTTTATTTTTCTTTAAAAACAACCAATTATTCAGTGACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                       >  1:686864/1‑150 (MQ=255)
                                                                               ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtata                                                                     >  1:1514582/1‑150 (MQ=255)
                                                                                cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                    <  1:373212/150‑1 (MQ=255)
                                                                                     aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTAAGTTACAACTGTTTGTACTCTCGCGGGGCAGGGATTAAACCAGcc                                                               >  2:1341374/1‑150 (MQ=255)
                                                                                         ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATTCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGATACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           <  1:1237293/150‑1 (MQ=255)
                                                                                         ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           >  1:851050/1‑150 (MQ=255)
                                                                                         ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           >  2:1702742/1‑150 (MQ=255)
                                                                                            tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        >  1:849962/1‑150 (MQ=255)
                                                                                            tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        <  2:1208930/150‑1 (MQ=255)
                                                                                             gCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgc                                                       <  1:1792036/150‑1 (MQ=255)
                                                                                              cGAAGCGCGTCACTATTTATTTTTTTCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgcc                                                      <  1:309384/150‑1 (MQ=255)
                                                                                               gAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgccg                                                     >  1:1792135/1‑150 (MQ=255)
                                                                                                   cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTATCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGc                                                 <  2:704013/150‑1 (MQ=255)
                                                                                                      gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  1:1465003/150‑1 (MQ=255)
                                                                                                      gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  2:1549999/150‑1 (MQ=255)
                                                                                                         aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAgg                                           <  1:1238139/150‑1 (MQ=255)
                                                                                                           tatttattTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgc                                         <  1:250823/150‑1 (MQ=255)
                                                                                                             tttatttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcca                                       <  1:1251119/150‑1 (MQ=255)
                                                                                                                 tttttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcca                                   <  2:232172/150‑1 (MQ=255)
                                                                                                                       tCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                             <  1:1562424/150‑1 (MQ=255)
                                                                                                                       tCTTAACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGATTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                             <  1:456267/150‑1 (MQ=255)
                                                                                                                        cTTTACCGTAAGAATGCAGAAACCGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAACCAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                            <  1:1646543/150‑1 (MQ=255)
                                                                                                                        cTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                            >  1:1222361/1‑150 (MQ=255)
                                                                                                                         tttACCGTAAGAATGCAGAAACAGCGTACCAGCCGCTGTTTCTTTTTATTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATATGGCCAGCCACGACCGcc                           <  2:1646453/150‑1 (MQ=255)
                                                                                                                         tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTGTTTAAAAACAATAAAATATTCAGTTAAAACTGTTTGTTCTCTCGCGGGGCCGGGATTATTCCAGCACCATCCGCCGTAGCCATAGGGCCACCCACGACCGcc                           >  2:1800615/1‑150 (MQ=255)
                                                                                                                            aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                        >  2:463630/1‑150 (MQ=255)
                                                                                                                            aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                        >  2:1412388/1‑150 (MQ=255)
                                                                                                                                tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACCACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:1446176/1‑150 (MQ=255)
                                                                                                                                tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  2:401541/150‑1 (MQ=255)
                                                                                                                                tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:808317/150‑1 (MQ=255)
                                                                                                                                tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:164597/1‑150 (MQ=255)
                                                                                                                                tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:2056/150‑1 (MQ=255)
                                                                                                                                     aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATgg               <  1:1129034/150‑1 (MQ=255)
                                                                                                                                     aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGGTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTCGCCAGAGGGCCAGCCCCGCCCGCCATATAGCCATgg               >  1:1719274/1‑150 (MQ=255)
                                                                                                                                             aCAGCGCACCTGCCGCAGTTTCGTTTTCTTTAATAACAAGCATTTTTTCTGTTTCAACTGTGTGTTCTCTCGCGGGGCGGGGATTATACCAGCCCCATCCGCCGTAGCCATTGGGCGAGCCACGACCGCCATAAAACCATGGATCAATCg       <  2:1640356/150‑1 (MQ=255)
                                                                                                                                               aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     >  2:223352/1‑150 (MQ=255)
                                                                                                                                               aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  1:45605/150‑1 (MQ=255)
                                                                                                                                               aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  2:972943/150‑1 (MQ=255)
                                                                                                                                               aGCGGACCAGACGCTGTTTCGTTTTCTTTAAAAAAAAGCTATTATTCAGTTACAACTGTTTGTACTCTCGACGGGCCGGGATTATACAACACCCATCGGCCGTATCCATAGGGCCCGCCACGCCCGCCAGAAACACATGGAGAAATAGGc     >  2:1586472/1‑150 (MQ=255)
                                                                                                                                                gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:501470/1‑150 (MQ=255)
                                                                                                                                                gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:1074230/1‑150 (MQ=255)
                                                                                                                                                  ggACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCACCCCCATACGCCGTAGCCATAGGGCCCGCCACGACCGCCATAAAACCATGGATCAATCGGCTGa  >  2:189931/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889767‑1890062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: