Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,917 C→G 5.2% intergenic (‑171/+34) fadD ← / ← yeaY acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,9170CG 5.2% 352.9 / 9.8 116intergenic (‑171/+34)fadD/yeaYacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base C (45/65);  new base G (2/4);  total (47/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-01

Only 100 of 118 total aligned reads displayed.

GTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889768‑1890062
                                                                                                                                                     |                                                                                                                                                 
gtTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAcc                                                                                                                                                   <  1:445821/150‑1 (MQ=255)
gtTCGGCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAcc                                                                                                                                                   <  2:76648/150‑1 (MQ=255)
      cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                             >  1:1763683/1‑150 (MQ=255)
       gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            >  1:1524426/1‑150 (MQ=255)
       gtaaTCTCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGATTTATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            <  1:160147/147‑1 (MQ=255)
            cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGCAACAGCGGCTGGTCCGCTGtttc                                                                                                                                       <  1:1437763/150‑1 (MQ=255)
            cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGGCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttc                                                                                                                                       <  1:1584079/150‑1 (MQ=255)
             gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttct                                                                                                                                      >  2:1756766/1‑150 (MQ=255)
              cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctt                                                                                                                                     >  1:1161682/1‑150 (MQ=255)
               aaCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctgc                                                                                                                                    <  2:1493776/150‑3 (MQ=255)
                aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  1:1774150/1‑150 (MQ=255)
                aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  2:1311598/1‑150 (MQ=255)
                 ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                  <  1:369550/150‑1 (MQ=255)
                 ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttattttt                                                                                                                                  >  1:69604/1‑150 (MQ=255)
                 ccccAAATGATATACATGCAGTTAACATAATATAAACTCATCATACCAGCTTGATAATTACCCAACTTAAAGGGTGCGAAGCGCGTCACTATTTATTTTAATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCTGTGtttcttttt                                                                                                                                  <  1:816197/150‑1 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1790321/150‑1 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 >  2:2056/1‑150 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  2:855569/150‑1 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1049995/150‑1 (MQ=255)
                     aaaTGATATACATGCCGTTAACATTATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  2:277518/150‑1 (MQ=255)
                     aaaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  1:524478/150‑1 (MQ=255)
                      aaTTATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1234389/150‑1 (MQ=255)
                      aaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1725251/150‑1 (MQ=255)
                       aTGATATACATGCAGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaa                                                                                                                            <  1:251054/150‑1 (MQ=255)
                        tGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaa                                                                                                                           <  2:983193/150‑1 (MQ=255)
                                aTGCCTTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGGCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCa                                                                                                                   <  1:1219330/150‑1 (MQ=255)
                                  tcagTTAACATAATATTAACTAATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCTAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAat                                                                                                                 <  2:1149062/147‑1 (MQ=255)
                                          cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGt                                                                                                         >  1:548395/1‑150 (MQ=255)
                                          cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGt                                                                                                         >  1:401541/1‑150 (MQ=255)
                                          cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGATTCTTATTCTTTAAAAACAAGCAATTATTCAGt                                                                                                         >  2:126922/1‑150 (MQ=255)
                                           ataataTTAACTCATCATTCCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAAGTATTCAGtt                                                                                                        >  2:149267/1‑150 (MQ=255)
                                           ataataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGtt                                                                                                        >  2:438927/1‑150 (MQ=255)
                                            taataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTa                                                                                                       <  2:1175713/150‑1 (MQ=255)
                                               tatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACaa                                                                                                    >  1:665117/1‑150 (MQ=255)
                                                atTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAATAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAAc                                                                                                   <  2:321226/150‑1 (MQ=255)
                                                   aaCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGt                                                                                                <  1:187253/150‑1 (MQ=255)
                                                    aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                               >  1:597821/1‑150 (MQ=255)
                                                    aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                               >  1:395352/1‑150 (MQ=255)
                                                     cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:1119713/150‑1 (MQ=255)
                                                     cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:981660/150‑1 (MQ=255)
                                                       catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCCGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGt                                                                                            >  2:61034/1‑150 (MQ=255)
                                                        atcatACCAGCTTGATAATTAACCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTa                                                                                           <  1:252613/150‑1 (MQ=255)
                                                           atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTActc                                                                                        >  2:1454376/1‑150 (MQ=255)
                                                              ccAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcg                                                                                     <  1:1290168/150‑1 (MQ=255)
                                                                  cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 <  2:661986/150‑1 (MQ=255)
                                                                  cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 >  1:1570306/1‑150 (MQ=255)
                                                                    tGATAATTACCCAACGAAACGGTTGCGAAGCGCTGCACTATTTATTTTTATCTTTCCGGTATGAATGGAGACACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                               <  2:963068/150‑1 (MQ=255)
                                                                      aTAATTACCCAACGAAAAGTTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCg                                                                             <  2:183074/150‑1 (MQ=255)
                                                                        aaTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                           <  2:610917/150‑1 (MQ=255)
                                                                         aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                          <  2:14024/150‑1 (MQ=255)
                                                                          ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                         <  2:948526/150‑1 (MQ=255)
                                                                           tACCCAACGAACAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTCAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  2:272951/150‑1 (MQ=255)
                                                                           tACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  1:618851/150‑1 (MQ=255)
                                                                            aCCCAACGAAATGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                       <  2:1084361/150‑1 (MQ=255)
                                                                            aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCAGTTTATTTTTCTTTAAAAACAACCAATTATTCAGTGACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                       >  1:686864/1‑150 (MQ=255)
                                                                              ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtata                                                                     >  1:901302/1‑150 (MQ=255)
                                                                               cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                    <  1:373212/150‑1 (MQ=255)
                                                                                    aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTAAGTTACAACTGTTTGTACTCTCGCGGGGCAGGGATTAAACCAGcc                                                               >  2:1341374/1‑150 (MQ=255)
                                                                                     aaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGccc                                                              >  1:1246562/1‑150 (MQ=255)
                                                                                       aGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCa                                                            <  2:17568/150‑1 (MQ=255)
                                                                                        ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATTCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGATACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           <  1:1237293/150‑1 (MQ=255)
                                                                                        ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           >  2:1702742/1‑150 (MQ=255)
                                                                                        ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           >  1:851050/1‑150 (MQ=255)
                                                                                           tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        >  1:849962/1‑150 (MQ=255)
                                                                                           tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        <  2:1208930/150‑1 (MQ=255)
                                                                                              gAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgccg                                                     >  1:1792135/1‑150 (MQ=255)
                                                                                                  cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTATCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGc                                                 <  2:704013/150‑1 (MQ=255)
                                                                                                   gcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCATTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGACGTAGcc                                                <  2:1161682/150‑1 (MQ=255)
                                                                                                     gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  1:1465003/150‑1 (MQ=255)
                                                                                                     gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  2:1549999/150‑1 (MQ=255)
                                                                                                        aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAgg                                           <  1:1238139/150‑1 (MQ=255)
                                                                                                          tatttattTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgc                                         <  1:250823/150‑1 (MQ=255)
                                                                                                            tttatttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcca                                       <  1:1251119/150‑1 (MQ=255)
                                                                                                                tttttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcca                                   <  2:232172/150‑1 (MQ=255)
                                                                                                                      tCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                             <  1:1562424/150‑1 (MQ=255)
                                                                                                                      tCTTAACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGATTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                             <  1:456267/150‑1 (MQ=255)
                                                                                                                       cTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                            >  1:1222361/1‑150 (MQ=255)
                                                                                                                        tttACCGTAAGAATGCAGAAACAGCGTACCAGCCGCTGTTTCTTTTTATTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATATGGCCAGCCACGACCGcc                           <  2:1646453/150‑1 (MQ=255)
                                                                                                                        tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTGTTTAAAAACAATAAAATATTCAGTTAAAACTGTTTGTTCTCTCGCGGGGCCGGGATTATTCCAGCACCATCCGCCGTAGCCATAGGGCCACCCACGACCGcc                           >  2:1800615/1‑150 (MQ=255)
                                                                                                                          tACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAAATATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCAt                         <  2:1637497/150‑1 (MQ=255)
                                                                                                                           aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                        >  2:1412388/1‑150 (MQ=255)
                                                                                                                               tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACCACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:1446176/1‑150 (MQ=255)
                                                                                                                               tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  2:401541/150‑1 (MQ=255)
                                                                                                                               tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:808317/150‑1 (MQ=255)
                                                                                                                               tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:2056/150‑1 (MQ=255)
                                                                                                                               tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:164597/1‑150 (MQ=255)
                                                                                                                                  gAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                 >  1:357541/1‑150 (MQ=255)
                                                                                                                                  agaaaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                 >  1:1172008/7‑150 (MQ=255)
                                                                                                                                    aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATgg               <  1:1129034/150‑1 (MQ=255)
                                                                                                                                    aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGGTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTCGCCAGAGGGCCAGCCCCGCCCGCCATATAGCCATgg               >  1:1719274/1‑150 (MQ=255)
                                                                                                                                     aaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCCCGACCGCCATAACACCATggt              >  2:906295/4‑149 (MQ=255)
                                                                                                                                       cAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATc            <  1:1454376/150‑1 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     >  2:223352/1‑150 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  1:45605/150‑1 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  1:1756766/150‑1 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  2:972943/150‑1 (MQ=255)
                                                                                                                                              aGCGGACCAGACGCTGTTTCGTTTTCTTTAAAAAAAAGCTATTATTCAGTTACAACTGTTTGTACTCTCGACGGGCCGGGATTATACAACACCCATCGGCCGTATCCATAGGGCCCGCCACGCCCGCCAGAAACACATGGAGAAATAGGc     >  2:1586472/1‑150 (MQ=255)
                                                                                                                                               gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:501470/1‑150 (MQ=255)
                                                                                                                                               gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:1074230/1‑150 (MQ=255)
                                                                                                                                                 ggACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCACCCCCATACGCCGTAGCCATAGGGCCCGCCACGACCGCCATAAAACCATGGATCAATCGGCTGa  >  2:189931/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889768‑1890062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: