Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,918 A→G 5.2% intergenic (‑172/+33) fadD ← / ← yeaY acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,9180AG 5.2% 343.6 / 7.1 116intergenic (‑172/+33)fadD/yeaYacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base A (44/64);  new base G (2/4);  total (47/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

Only 100 of 116 total aligned reads displayed.

GTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889772‑1890062
                                                                                                                                                  |                                                                                                                                                
gtcgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGcc                                                                                                                                               <  2:1046196/150‑1 (MQ=255)
  cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                             >  1:1763683/1‑150 (MQ=255)
   gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            >  1:1524426/1‑150 (MQ=255)
   gtaaTCTCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGATTTATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                            <  1:160147/147‑1 (MQ=255)
       tCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGttt                                                                                                                                        >  2:1421613/1‑150 (MQ=255)
        cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGCAACAGCGGCTGGTCCGCTGtttc                                                                                                                                       <  1:1437763/150‑1 (MQ=255)
        cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGGCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttc                                                                                                                                       <  1:1584079/150‑1 (MQ=255)
        cGCAACCCCAAATGATATACAAGCCGTTAACATAATATTAACTCATCATACCAGCTTGCTAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttc                                                                                                                                       <  2:902329/150‑1 (MQ=255)
         gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttct                                                                                                                                      <  1:438713/150‑1 (MQ=255)
         gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttct                                                                                                                                      >  2:1756766/1‑150 (MQ=255)
          cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctt                                                                                                                                     >  1:1161682/1‑150 (MQ=255)
           aaCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctgc                                                                                                                                    <  2:1493776/150‑3 (MQ=255)
            aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  1:1774150/1‑150 (MQ=255)
            aCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttt                                                                                                                                   >  2:1311598/1‑150 (MQ=255)
             ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                  <  1:369550/150‑1 (MQ=255)
             ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttattttt                                                                                                                                  >  1:69604/1‑150 (MQ=255)
             ccccAAATGATATACATGCAGTTAACATAATATAAACTCATCATACCAGCTTGATAATTACCCAACTTAAAGGGTGCGAAGCGCGTCACTATTTATTTTAATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCTGTGtttcttttt                                                                                                                                  <  1:816197/150‑1 (MQ=255)
              cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1049995/150‑1 (MQ=255)
              cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                 <  1:1790321/150‑1 (MQ=255)
                cAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttctt                                                                                                                               >  2:1251119/1‑150 (MQ=255)
                 aaaTGATATACATGCCGTTAACATTATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  2:277518/150‑1 (MQ=255)
                 aaaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                              <  1:524478/150‑1 (MQ=255)
                  aaTTATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1234389/150‑1 (MQ=255)
                  aaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                             <  2:1725251/150‑1 (MQ=255)
                   aTGATATACATGCAGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaa                                                                                                                            <  1:251054/150‑1 (MQ=255)
                    tGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaa                                                                                                                           <  2:983193/150‑1 (MQ=255)
                    tGAAATACATGCCGTAAACATAATATTAACTCATCATACCAGCTTGAAAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTAAACCCTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaa                                                                                                                           <  1:1210884/150‑1 (MQ=255)
                         taCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACaa                                                                                                                      >  2:250823/1‑150 (MQ=255)
                              tcagTTAACATAATATTAACTAATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCTAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAat                                                                                                                 <  2:1149062/147‑1 (MQ=255)
                                      cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGt                                                                                                         >  1:401541/1‑150 (MQ=255)
                                       ataataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGtt                                                                                                        >  2:438927/1‑150 (MQ=255)
                                        taataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTa                                                                                                       <  2:1175713/150‑1 (MQ=255)
                                           tatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACaa                                                                                                    >  1:665117/1‑150 (MQ=255)
                                            atTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAATAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAAc                                                                                                   <  2:321226/150‑1 (MQ=255)
                                               aaCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGt                                                                                                <  1:187253/150‑1 (MQ=255)
                                                aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                               >  1:597821/1‑150 (MQ=255)
                                                 cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:981660/150‑1 (MQ=255)
                                                 cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  2:1761310/150‑1 (MQ=255)
                                                 cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                              <  1:1119713/150‑1 (MQ=255)
                                                   catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCCGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGt                                                                                            >  2:61034/1‑150 (MQ=255)
                                                    atcatACCAGCTTGATAATTAACCAACGAAAAGGTTGCGAAGCGCGTCACTATTTTTTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTa                                                                                           <  1:252613/150‑1 (MQ=255)
                                                       atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCTCGTCACAATATAAATTTATCTTAACCGTAAGAATTCAGAAACACCGGACCAGCCGCTGATTCTTTATATGTAAAAACAAGCAAATATACAGTTAAAAAAGTTTGTtctc                                                                                        >  1:1267641/1‑150 (MQ=255)
                                                       atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTActc                                                                                        >  2:1454376/1‑150 (MQ=255)
                                                              cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 >  1:1570306/1‑150 (MQ=255)
                                                              cTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCggg                                                                                 <  2:661986/150‑1 (MQ=255)
                                                                tGATAATTACCCAACGAAACGGTTGCGAAGCGCTGCACTATTTATTTTTATCTTTCCGGTATGAATGGAGACACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                               <  2:963068/150‑1 (MQ=255)
                                                                  aTAATTACCCAACGAAAAGTTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCg                                                                             <  2:183074/150‑1 (MQ=255)
                                                                    aaTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                           <  2:610917/150‑1 (MQ=255)
                                                                     aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                          <  2:14024/150‑1 (MQ=255)
                                                                      ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                         <  2:948526/150‑1 (MQ=255)
                                                                      ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                         <  1:586411/150‑1 (MQ=255)
                                                                       tACCCAACGAACAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTCAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  2:272951/150‑1 (MQ=255)
                                                                       tACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                        <  1:618851/150‑1 (MQ=255)
                                                                        aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCAGTTTATTTTTCTTTAAAAACAACCAATTATTCAGTGACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                       >  1:686864/1‑150 (MQ=255)
                                                                          ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtata                                                                     >  1:1514582/1‑150 (MQ=255)
                                                                          ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtata                                                                     >  1:901302/1‑150 (MQ=255)
                                                                           cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                    <  1:373212/150‑1 (MQ=255)
                                                                                aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTAAGTTACAACTGTTTGTACTCTCGCGGGGCAGGGATTAAACCAGcc                                                               >  2:1341374/1‑150 (MQ=255)
                                                                                 aaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGccc                                                              >  1:1246562/1‑150 (MQ=255)
                                                                                   aGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCa                                                            <  2:17568/150‑1 (MQ=255)
                                                                                    ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATTCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGATACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           <  1:1237293/150‑1 (MQ=255)
                                                                                    ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCAt                                                           >  1:851050/1‑150 (MQ=255)
                                                                                       tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        >  1:849962/1‑150 (MQ=255)
                                                                                       tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                        <  2:1208930/150‑1 (MQ=255)
                                                                                        gCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgc                                                       <  1:1792036/150‑1 (MQ=255)
                                                                                         cGAAGCGCGTCACTATTTATTTTTTTCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgcc                                                      <  1:309384/150‑1 (MQ=255)
                                                                                          gAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgccg                                                     >  1:1792135/1‑150 (MQ=255)
                                                                                              cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTATCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGc                                                 <  2:704013/150‑1 (MQ=255)
                                                                                               gcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCATTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGACGTAGcc                                                <  2:1161682/150‑1 (MQ=255)
                                                                                                 gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  2:1549999/150‑1 (MQ=255)
                                                                                                 gTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCAt                                              <  1:1465003/150‑1 (MQ=255)
                                                                                                    aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAgg                                           <  1:1238139/150‑1 (MQ=255)
                                                                                                        tttatttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcca                                       <  1:1251119/150‑1 (MQ=255)
                                                                                                            tttttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcca                                   <  2:232172/150‑1 (MQ=255)
                                                                                                                  tCTTAACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGATTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCg                             <  1:456267/150‑1 (MQ=255)
                                                                                                                   cTTTACCGTAAGAATGCAGAAACCGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAACCAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                            <  1:1646543/150‑1 (MQ=255)
                                                                                                                   cTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                            >  1:1222361/1‑150 (MQ=255)
                                                                                                                    tttACCGTAAGAATGCAGAAACAGCGTACCAGCCGCTGTTTCTTTTTATTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATATGGCCAGCCACGACCGcc                           <  2:1646453/150‑1 (MQ=255)
                                                                                                                    tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTGTTTAAAAACAATAAAATATTCAGTTAAAACTGTTTGTTCTCTCGCGGGGCCGGGATTATTCCAGCACCATCCGCCGTAGCCATAGGGCCACCCACGACCGcc                           >  2:1800615/1‑150 (MQ=255)
                                                                                                                      tACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAAATATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCAt                         <  2:1637497/150‑1 (MQ=255)
                                                                                                                       aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                        >  2:1412388/1‑150 (MQ=255)
                                                                                                                       aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                        >  2:463630/1‑150 (MQ=255)
                                                                                                                           tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACCACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:1446176/1‑150 (MQ=255)
                                                                                                                           tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:808317/150‑1 (MQ=255)
                                                                                                                           tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    <  1:2056/150‑1 (MQ=255)
                                                                                                                           tAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                    >  1:164597/1‑150 (MQ=255)
                                                                                                                              gAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                 >  1:357541/1‑150 (MQ=255)
                                                                                                                              agaaaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                 >  1:1172008/7‑150 (MQ=255)
                                                                                                                                aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATgg               <  1:1129034/150‑1 (MQ=255)
                                                                                                                                aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGGTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTCGCCAGAGGGCCAGCCCCGCCCGCCATATAGCCATgg               >  1:1719274/1‑150 (MQ=255)
                                                                                                                                 aaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCCCGACCGCCATAACACCATggt              >  2:906295/4‑149 (MQ=255)
                                                                                                                                   cAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATc            <  1:1454376/150‑1 (MQ=255)
                                                                                                                                        aCAGCGCACCTGCCGCAGTTTCGTTTTCTTTAATAACAAGCATTTTTTCTGTTTCAACTGTGTGTTCTCTCGCGGGGCGGGGATTATACCAGCCCCATCCGCCGTAGCCATTGGGCGAGCCACGACCGCCATAAAACCATGGATCAATCg       <  2:1640356/150‑1 (MQ=255)
                                                                                                                                          aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     >  2:223352/1‑150 (MQ=255)
                                                                                                                                          aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  1:45605/150‑1 (MQ=255)
                                                                                                                                          aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc     <  2:972943/150‑1 (MQ=255)
                                                                                                                                          aGCGGACCAGACGCTGTTTCGTTTTCTTTAAAAAAAAGCTATTATTCAGTTACAACTGTTTGTACTCTCGACGGGCCGGGATTATACAACACCCATCGGCCGTATCCATAGGGCCCGCCACGCCCGCCAGAAACACATGGAGAAATAGGc     >  2:1586472/1‑150 (MQ=255)
                                                                                                                                           gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:501470/1‑150 (MQ=255)
                                                                                                                                           gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt    >  1:1074230/1‑150 (MQ=255)
                                                                                                                                             ggACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCACCCCCATACGCCGTAGCCATAGGGCCCGCCACGACCGCCATAAAACCATGGATCAATCGGCTGa  >  2:189931/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGA  >  NC_000913/1889772‑1890062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: