Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 285,202 T→A 5.1% intergenic (+34/‑193) yagF → / → yagG CP4‑6 prophage; dehydratase family protein/CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913285,2020TA 5.1% 338.8 / 3.4 118intergenic (+34/‑193)yagF/yagGCP4‑6 prophage; dehydratase family protein/CP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base T (42/69);  new base A (3/4);  total (45/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACTCCA  >  NC_000913/285053‑285347
                                                                                                                                                     |                                                                                                                                                 
cccGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATAtt                                                                                                                                                   >  1:1421588/1‑150 (MQ=255)
 ccGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGCCGGTATTTATGACCCCGATAAAATTATCGAGGTAATTAACGCCGGTAAAACCGCGCTCGGAATTTAATTATTTGAAGAGATAAAACCGTCTGCGGAATAttt                                                                                                                                                  >  1:388162/1‑150 (MQ=255)
     cTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCg                                                                                                                                              <  1:1775629/150‑1 (MQ=255)
     cTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCg                                                                                                                                              <  1:510490/150‑1 (MQ=255)
       gtgGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTTACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCa                                                                                                                                            >  1:1102773/1‑150 (MQ=255)
       gtgGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCa                                                                                                                                            >  1:1281577/1‑150 (MQ=255)
          ggcggtACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGAc                                                                                                                                         <  1:825436/150‑1 (MQ=255)
          ggcggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGAc                                                                                                                                         <  2:1567044/150‑1 (MQ=255)
          ggcggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTAATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGAc                                                                                                                                         <  1:1586766/150‑1 (MQ=255)
           gcggcACTGCAGGCGGGGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACg                                                                                                                                        <  2:952951/150‑1 (MQ=255)
                    cAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTttgttg                                                                                                                               >  2:690729/1‑150 (MQ=255)
                    cAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTttgttg                                                                                                                               <  2:510529/150‑1 (MQ=255)
                        cGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGtttt                                                                                                                           <  1:1336225/150‑1 (MQ=255)
                           tGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGa                                                                                                                        >  2:550604/1‑150 (MQ=255)
                           tGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGa                                                                                                                        <  2:1661874/150‑1 (MQ=255)
                            gAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGaa                                                                                                                       <  2:18563/150‑1 (MQ=255)
                             aGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGATTTTAATTATTTTAAGAGATAAAACAGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGaaa                                                                                                                      >  2:885291/1‑150 (MQ=255)
                             aGCGGCGGCACCTGGAAAGGCTGTATTTATGAAACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAAGTTAATTATTTTAAGAGATAAAACCGGCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGaaa                                                                                                                      <  1:859126/150‑1 (MQ=255)
                              gcggcgGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAAt                                                                                                                     <  1:700811/150‑1 (MQ=255)
                               cggcggCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAAtt                                                                                                                    <  2:1564189/150‑1 (MQ=255)
                                ggcggcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAAttt                                                                                                                   <  2:1421588/150‑1 (MQ=255)
                                 gcggcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATtta                                                                                                                  <  2:547011/150‑1 (MQ=255)
                                   ggcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATttatt                                                                                                                <  2:133793/150‑1 (MQ=255)
                                   ggcACATGGAAATGCTGTATTTATGACACCGATTTAATTATCGAGGTAATTAACGCCGGTATAAATGCGGTCGGTATTTTTTTATTTTAAGAGATAAAACCGTCTTCGCAATATTTCCCGCAGACTGCTTTGTTTTTTTTGAAATttatt                                                                                                                <  2:1226792/150‑1 (MQ=255)
                                    gcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATttatta                                                                                                               <  2:1411472/150‑1 (MQ=255)
                                     cACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTaa                                                                                                              >  1:1613592/1‑150 (MQ=255)
                                       ccTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAAtt                                                                                                            >  2:1319901/1‑150 (MQ=255)
                                        attgAAAGGCTGTATTTATGACACAGATAAAATTATCGAGGTAATTAACGCCGGTAAAATAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTGTTGAAATTTATTAAttt                                                                                                           <  1:78963/147‑1 (MQ=255)
                                           gAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                        <  2:1524199/150‑1 (MQ=255)
                                            aaaGGCTGTATTTATGACCCCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAAc                                                                                                       <  2:944454/150‑1 (MQ=255)
                                             aaGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACa                                                                                                      <  2:1288503/150‑1 (MQ=255)
                                                gCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAAtt                                                                                                   <  1:1218723/150‑1 (MQ=255)
                                                gCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAAtt                                                                                                   <  2:1378809/150‑1 (MQ=255)
                                                   ttATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGt                                                                                                <  1:784843/149‑1 (MQ=255)
                                                     aTTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTg                                                                                              <  1:1005189/150‑1 (MQ=255)
                                                       ttATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTgag                                                                                            <  1:420231/150‑1 (MQ=255)
                                                          tGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATATAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGata                                                                                         <  2:1085526/150‑1 (MQ=255)
                                                          tGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGata                                                                                         <  2:708515/150‑1 (MQ=255)
                                                           gACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatat                                                                                        >  2:491957/1‑150 (MQ=255)
                                                           gACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatat                                                                                        >  1:681227/1‑150 (MQ=255)
                                                             cacCGATAAAATTATCGAGGTAATTAACGCCGGTAAAACAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatatat                                                                                      <  2:141030/150‑1 (MQ=255)
                                                               cctaTAAAATTATCGAGGTAATTAACGCCTGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCg                                                                                    <  1:102837/147‑1 (MQ=255)
                                                               ccGATAAAATTATCGATGTAATTAACGCCTGTAAAAAAGCTCTCGGAATTTTATTATTTTAAGAGATAAAACCGTCTGAGGAATAGTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTAATTAATTTAAACAATTAGTTGAGATATATCg                                                                                    <  1:274239/150‑1 (MQ=255)
                                                               ccGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCg                                                                                    <  1:977942/150‑1 (MQ=255)
                                                                 gATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATATCCCTCAGACCGCTGTGTTGTTTTAGAAATTTAGTAATTTAAACAATAAGTTGAGATATATCGtt                                                                                  >  1:358178/1‑150 (MQ=255)
                                                                     aaaTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATAGTTGGCg                                                                              >  1:1042238/1‑150 (MQ=255)
                                                                      aaTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGt                                                                             <  2:390162/150‑1 (MQ=255)
                                                                         tataGAGTTAATTAACGAATGTAAAAAATCGATCGGAATTTAATTATTTTAATAGATAAAACCTTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATAAATCGTTGGCGTcac                                                                          <  1:871123/150‑1 (MQ=255)
                                                                         tATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTcac                                                                          <  2:1035081/150‑1 (MQ=255)
                                                                             gAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaaaa                                                                      >  2:1486301/1‑150 (MQ=255)
                                                                              agttAATTAACGCCGGTAAAAAAGCTCTCTGAATTTAATTATTTTAATATATAAAACCGTCTGCGGAATATATCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAg                                                                     <  1:204192/147‑1 (MQ=255)
                                                                              aGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTAATTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTAGAGATATATCGTTGGCGTCACAAAAg                                                                     <  2:936475/150‑1 (MQ=255)
                                                                               ggTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGc                                                                    <  2:276157/150‑1 (MQ=255)
                                                                                 tAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCaa                                                                  <  1:1486301/150‑1 (MQ=255)
                                                                                   aTTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCaaaa                                                                <  2:1225683/150‑1 (MQ=255)
                                                                                     tAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGCCGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATa                                                              >  1:1726913/1‑150 (MQ=255)
                                                                                       aCGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAAc                                                            >  2:697996/1‑150 (MQ=255)
                                                                                          ccGGTAAAAAAGCGCTCGGAATTAAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTAATAATTTAAACAATTAGTTGAGATATATCGCTGGCGTCACAAAAGCAAAAAAACGTa                                                         <  2:592189/150‑1 (MQ=255)
                                                                                            ggTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTTTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAAt                                                       <  1:1677208/150‑1 (MQ=255)
                                                                                              tAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGAAAAATAACGTAAtta                                                     >  1:1522554/1‑149 (MQ=255)
                                                                                               aaaaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCg                                                    >  2:348565/1‑150 (MQ=255)
                                                                                                aaaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCgg                                                   <  2:887781/150‑1 (MQ=255)
                                                                                                 aaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGa                                                  >  2:1706746/1‑150 (MQ=255)
                                                                                                 aaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGa                                                  <  2:36301/150‑1 (MQ=255)
                                                                                                 aaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGa                                                  <  2:1036377/150‑1 (MQ=255)
                                                                                                 aaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGAAAAATAACGTAATTCGGa                                                  >  1:754909/1‑150 (MQ=255)
                                                                                                   aaGCTCTCGGAATTTAATTATTTTATGAGATAAAAACGTCTGCGGAATATTTCCCGGATACGGCTTTGTTGTTTTTGAAATTAAATAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGaaa                                                <  1:171783/150‑1 (MQ=255)
                                                                                                      cgcTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTTGCGTAAAAAAACAAAAATAAAATAATTCGGAAATaa                                             >  1:1298638/1‑150 (MQ=255)
                                                                                                      cgcTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCTGAAATaa                                             >  2:808928/1‑150 (MQ=255)
                                                                                                      cgcTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGAGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATaa                                             <  2:1613592/150‑1 (MQ=255)
                                                                                                           ggAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGatat                                        >  1:985012/1‑150 (MQ=255)
                                                                                                             aaTTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGa                                      <  2:664458/150‑1 (MQ=255)
                                                                                                              aTTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGAc                                     <  1:1586488/150‑1 (MQ=255)
                                                                                                              aTTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGAc                                     <  2:809184/150‑1 (MQ=255)
                                                                                                                 tAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCAt                                  >  2:1755736/1‑150 (MQ=255)
                                                                                                                   attattTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTGACAAAAGGATAATAACGTAATTCGGATATTAGTTTTGAcgtttg                                >  1:395899/1‑145 (MQ=255)
                                                                                                                   attattTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTAAAAAAAGAAAAATAACGTAATTAGGATATAAGATATGACCTTTg                                >  1:1731773/1‑150 (MQ=255)
                                                                                                                    ttattTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGc                               <  1:1319901/150‑1 (MQ=255)
                                                                                                                     tattTTAAGAGATAAAACCTTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCt                              <  1:1431081/150‑1 (MQ=255)
                                                                                                                     tattTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCt                              >  1:1431028/1‑150 (MQ=255)
                                                                                                                      attTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTg                             >  1:1456135/1‑150 (MQ=255)
                                                                                                                         ttAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATACGGAAATAAGATATGACCATTGCTGGtt                          <  2:757750/150‑1 (MQ=255)
                                                                                                                          tAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCATAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTa                         >  2:975496/1‑150 (MQ=255)
                                                                                                                          tAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTa                         <  1:941324/150‑1 (MQ=255)
                                                                                                                             gagaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTGACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAAtt                      >  1:469580/1‑150 (MQ=255)
                                                                                                                             gagaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAAtt                      <  1:491957/150‑1 (MQ=255)
                                                                                                                              agaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTg                     <  1:470317/150‑1 (MQ=255)
                                                                                                                              agaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTg                     <  1:1055402/150‑1 (MQ=255)
                                                                                                                              agaTAAAACATTATGAGGAATATTTCCTTCAGACGGATTTGTTGTTTTTGATATTTATTAATTTAAACAATTATTTGTGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTg                     <  1:97180/150‑1 (MQ=255)
                                                                                                                                  aaagCCGTCTGCGGGAAATATTCCGCAGACGGTTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACAATTGCTGGTTAATTGAATa                 >  2:1319501/1‑150 (MQ=39)
                                                                                                                                    aaCCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGc               >  2:1567578/1‑150 (MQ=255)
                                                                                                                                      ccGTCTGCCGAATATATCCCGCAGACGGCTTTGTTGTTTTTGAAATATATTAATTTAAACAATTAGTTGAGAAATATCGTTGGCGTCACAAAAGCAAAATCACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTc             <  2:19645/150‑1 (MQ=255)
                                                                                                                                       cGTCTGCAGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCa            >  2:1411507/1‑150 (MQ=255)
                                                                                                                                         tCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACTTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCAtt          >  1:1432795/1‑150 (MQ=255)
                                                                                                                                          attatgAATATTTCCCTCAGACTTATTTGTTGTTTTTGAAATTTATTAATTTAAAAAATTAGTTGAGATATATCGTTGGCGTCTCAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTa         <  1:268080/145‑1 (MQ=255)
                                                                                                                                             cGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTacac      <  2:1102773/150‑1 (MQ=255)
                                                                                                                                              ggAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAAAGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACt     >  1:770114/1‑150 (MQ=255)
                                                                                                                                                aaTATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACTcc   <  2:1187807/150‑1 (MQ=255)
                                                                                                                                                aaTATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACTcc   <  1:550604/150‑1 (MQ=255)
                                                                                                                                                 atatTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACTCCa  >  2:844251/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACTCCA  >  NC_000913/285053‑285347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: