Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,932,095 T→A 6.2% intergenic (+36/+20) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,0950TA 6.2% 348.6 / 7.8 120intergenic (+36/+20)purT/edaphosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
Reads supporting (aligned to +/- strand):  ref base T (52/58);  new base A (6/2);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 121 total aligned reads displayed.

GGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTAG  >  NC_000913/1931948‑1932244
                                                                                                                                                   |                                                                                                                                                     
ggAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCg                                                                                                                                                     <  1:34330/150‑1 (MQ=255)
    aTTGATCGCCGCCGTCGGCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCg                                                                                                                                                 <  2:1711497/150‑1 (MQ=255)
      tGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGTGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCggg                                                                                                                                               <  2:1675637/150‑1 (MQ=255)
      tGATGGCAGCCGACGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCggg                                                                                                                                               <  2:1787624/150‑1 (MQ=255)
       gATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                                                                              <  1:1211964/150‑1 (MQ=255)
       gATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                                                                              <  1:873016/150‑1 (MQ=255)
        aTGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                                                                                                             <  1:1538440/150‑1 (MQ=255)
         tGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGATCGCGCGAAGCACGCCGCCGGACAGGTTAAAGTACAGGGTTAAACCCGGCAAATAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAt                                                                                                                                            >  2:1565658/1‑150 (MQ=255)
         tGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAt                                                                                                                                            <  1:1125943/150‑1 (MQ=255)
          ggCAGCCGTCGTCGGGGGGGGGCACTGGCTACTGCTGAGAGGGTTGTTGACGCAATTGAACGCGAGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                                                                                                           <  2:931398/150‑1 (MQ=255)
          ggCAGCCGGCGGCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                                                                                                           <  2:1547337/150‑1 (MQ=255)
             aGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                                                                                                                        <  2:449884/150‑1 (MQ=255)
              gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa                                                                                                                                       >  1:1628675/1‑150 (MQ=255)
               ccGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAc                                                                                                                                      >  2:1447192/1‑150 (MQ=255)
                cgtcgtCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACt                                                                                                                                     <  1:1341438/150‑1 (MQ=255)
                 gtcgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGCCGCCATTGCACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCATAAAAACGCTACAAAAATGCCCGCTCCTCGCACGGCCATTTTGACtt                                                                                                                                    >  2:101950/1‑150 (MQ=255)
                 gtcggcGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                                                                                                                    <  1:1120745/150‑1 (MQ=255)
                  gcggcTGGGGGGGGCACTGGCTACTGCAGAGAGGGTGGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACttt                                                                                                                                   <  1:1716524/146‑1 (MQ=255)
                   cgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                                                                                                                  >  1:176960/1‑150 (MQ=255)
                    gtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                                                                                                                 >  2:37543/1‑150 (MQ=255)
                      cTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                                                                                                               <  1:857561/150‑1 (MQ=255)
                      cTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGATGACGCCATTGAACGCGCGAAGCACGCCGCCGTACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                                                                                                               >  1:1466308/1‑150 (MQ=255)
                       tGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAg                                                                                                                              <  1:953635/150‑1 (MQ=255)
                           ggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                                                                                                          <  1:1042264/150‑1 (MQ=255)
                                      cTACTGCTGAGAGTGTTGTTGTCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACTGGGTTATACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCAAc                                                                                                               <  2:604031/150‑1 (MQ=255)
                                      cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAc                                                                                                               <  2:611781/150‑1 (MQ=255)
                                      cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAc                                                                                                               <  2:401717/150‑1 (MQ=255)
                                         cTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTACACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCGCGCTCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                                                                                                            >  2:1360878/1‑150 (MQ=255)
                                           gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                                                                                                          <  1:1764997/150‑1 (MQ=255)
                                            cAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACATGGTTAAACCCGGAATAAAAACGATACAAAAATGCCCTATCCTCGATCGGGCATTTTGAGTTTTACAGCTTAGCGCCTTCTACCGCTTc                                                                                                         >  2:335034/1‑150 (MQ=255)
                                            cAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                                                                                                         >  1:32190/1‑150 (MQ=255)
                                             agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                                                                                                        >  1:1031450/1‑150 (MQ=255)
                                              tagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAc                                                                                                       <  1:1517907/149‑1 (MQ=255)
                                                gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCTGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc                                                                                                     >  2:411183/1‑150 (MQ=255)
                                                gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCTGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc                                                                                                     >  2:1749928/1‑150 (MQ=255)
                                                  gtgtGGTGGACGCCAGTGAACGCGCGAAGCACGACGCCGGACAGGTAAAAGTACAGGGTTATACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACgcgc                                                                                                   <  2:857852/150‑1 (MQ=255)
                                                   tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc                                                                                                  <  2:1570817/150‑1 (MQ=255)
                                                       gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                              <  2:274778/150‑1 (MQ=255)
                                                       gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                              <  2:737036/150‑1 (MQ=255)
                                                       gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                              >  1:983671/1‑150 (MQ=255)
                                                        ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                             <  1:1384725/150‑1 (MQ=255)
                                                         tgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa                                                                                            >  1:370149/1‑150 (MQ=255)
                                                           aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGt                                                                                          <  1:1035594/150‑1 (MQ=255)
                                                               cATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATg                                                                                      >  1:1478326/1‑150 (MQ=255)
                                                                 tttaaCTCGCGAAGCACGCCGGCGGACAGGTAAAAGTACACGGTTCAACCCGGCAAAAAAAAGATACAAAAATGCCCGATCCTCGCTCGGGCATTTTGACTTTTACCGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCg                                                                                    <  2:75979/147‑1 (MQ=255)
                                                                   gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                                                                                  >  1:816989/1‑150 (MQ=255)
                                                                     aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                                <  2:45508/150‑1 (MQ=255)
                                                                     aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                                >  2:1530620/1‑150 (MQ=255)
                                                                      cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTTCAAAAATGCCTGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                               >  2:370522/1‑150 (MQ=255)
                                                                      agcgcgAAGCACGCCGCCGGACAGGTAAAAGTACATGGTTAAACCCGGCAAAAAAACGCTACAAAAATCCCCGATCATCGATCGGTCATTTTGACTTTTACAGCTTATCGCCTTCTACAGCAAAACCAGCCAGCTTAGTAATCCGGTCga                                                                               >  2:914017/2‑149 (MQ=255)
                                                                        cgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTGGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTaa                                                                             >  2:47755/1‑150 (MQ=255)
                                                                        cgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTAACCGCTTAGCGCCTTCTACAGCTTCACGCGCCCGCTTAGTAATGCGGTCGTaa                                                                             >  1:1350546/1‑150 (MQ=255)
                                                                         gcgAAGCACGCCGCCGGACAGGTAAAAGTACTGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                            >  1:150935/1‑150 (MQ=255)
                                                                         gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAATAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                            <  1:380708/150‑1 (MQ=255)
                                                                           gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCg                                                                          <  2:1416975/150‑1 (MQ=255)
                                                                             aGCACGCCGCCGGACAGGTATAAGTACAGGGTTAAACCCGGCAAAAAAACGCAACAAAAATGCCCGATCCTCGTGCGGGCATTTTGACTTTTACAGCTTACCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                                                                        <  1:622833/150‑1 (MQ=255)
                                                                             aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                                                                        <  1:514672/150‑1 (MQ=255)
                                                                             aGCACCCCGCCGGACAGGTAAAAGTACAGTGTTAAACCCGTCAAAAAAACTCTACAAAAATGCCCGATCCTCCCTCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGTTCGTAATCTcc                                                                        <  2:115903/150‑1 (MQ=255)
                                                                              gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGATTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                                                                       >  1:1136116/1‑150 (MQ=255)
                                                                              gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCGCGATCGGGCATTTTGCCTTTGACAGCTTAACGCCTTCTACAGCTTCACGCGCCAGCTTAGAAATGCGGACGTAAACcccc                                                                       >  1:253781/1‑150 (MQ=255)
                                                                                 cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCt                                                                    >  2:817716/1‑150 (MQ=255)
                                                                                 cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCt                                                                    >  2:1811251/1‑150 (MQ=255)
                                                                                   ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTc                                                                  >  1:1139632/1‑150 (MQ=255)
                                                                                   ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTc                                                                  >  2:1007977/1‑150 (MQ=255)
                                                                                    cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTcc                                                                 <  1:969971/150‑1 (MQ=255)
                                                                                       cgGACAGGTAAAAGTACAGGGTTAAACCCGGAAAAAACACGCACCAAAAATCCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc                                                              <  1:1134582/150‑1 (MQ=255)
                                                                                         gACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgcgc                                                            <  2:796765/150‑1 (MQ=255)
                                                                                          aCAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCa                                                           <  2:1021624/150‑1 (MQ=255)
                                                                                             ggTAAAAGTACCGGGTTAAACCCGGCAAAAAAAAGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTCATCGCCCGCTTCCAGCGCATCt                                                        >  1:756081/1‑150 (MQ=255)
                                                                                             ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                        >  2:1077319/1‑150 (MQ=255)
                                                                                             ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                        >  2:1760347/1‑150 (MQ=255)
                                                                                               tAAAAGTACTGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGc                                                      >  2:1107737/1‑150 (MQ=255)
                                                                                               tAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGc                                                      <  1:1179734/150‑1 (MQ=255)
                                                                                                aaaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc                                                     >  1:1570876/1‑150 (MQ=255)
                                                                                                aaaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc                                                     >  2:155970/1‑150 (MQ=255)
                                                                                                   aGTACAGGGTTAAACCCGGCAAAAAAACGCTGCAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGa                                                  <  2:1381661/150‑1 (MQ=255)
                                                                                                    gTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGaa                                                 <  1:1442892/150‑1 (MQ=255)
                                                                                                      aCAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGATTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAcc                                               >  2:1043886/1‑150 (MQ=255)
                                                                                                          ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTCCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                           <  1:968690/150‑1 (MQ=255)
                                                                                                                aCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAac                                     >  2:1114676/1‑150 (MQ=255)
                                                                                                                 cccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAacc                                    >  1:841947/1‑150 (MQ=255)
                                                                                                                   cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccac                                  <  1:1447192/150‑1 (MQ=255)
                                                                                                                   cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccac                                  >  1:1430587/1‑150 (MQ=255)
                                                                                                                    ggCAAAAAAACGCTACAATAATGCCCGATCCTCGATCTGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGTAACCAGCCAGGAacgacg                                 <  2:194670/150‑2 (MQ=255)
                                                                                                                    ggCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccacc                                 <  2:370149/150‑1 (MQ=255)
                                                                                                                            aaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcaca                         <  2:380838/150‑1 (MQ=255)
                                                                                                                              cGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGc                       >  2:1294647/1‑150 (MQ=255)
                                                                                                                                 tACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACg                    >  1:1056509/1‑150 (MQ=255)
                                                                                                                                 agtcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGGGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAGCCCGCCAGGAACCACCGATGCACAGCACg                    >  1:1201992/5‑150 (MQ=255)
                                                                                                                                 agtcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCCCCGATGCACAGCACg                    >  1:1316980/5‑150 (MQ=255)
                                                                                                                                   cAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTACTCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCt                  <  2:1122489/150‑1 (MQ=255)
                                                                                                                                       aaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTc              >  1:954240/1‑150 (MQ=255)
                                                                                                                                        aTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCa             >  1:713708/1‑150 (MQ=255)
                                                                                                                                         tGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCTCTGCACAGCACGCTTTTCAg            >  1:1363277/1‑150 (MQ=255)
                                                                                                                                          gCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgc           >  1:889769/1‑150 (MQ=255)
                                                                                                                                          gCCCGATCCTCGATCGGGCATTTTGACATTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgc           >  1:1548146/1‑150 (MQ=255)
                                                                                                                                            ccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgcgc         >  1:1271415/1‑150 (MQ=255)
                                                                                                                                             cGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGACCCACCGATGCACAGCACGCTTTTCAGCGcc        >  1:1338277/1‑150 (MQ=255)
                                                                                                                                               aTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCCCCGATGCACAGCCCGCTTTTCAGCGCCAg      >  1:1150720/1‑150 (MQ=255)
                                                                                                                                                  cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTa   >  2:171935/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTAG  >  NC_000913/1931948‑1932244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: