Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,982,500 T→C 5.3% intergenic (‑113/+54) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,982,5000TC 5.3% 320.6 / 5.6 112intergenic (‑113/+54)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (50/55);  new base C (4/2);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 113 total aligned reads displayed.

CGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAG  >  NC_000913/1982351‑1982645
                                                                                                                                                     |                                                                                                                                                 
cgGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGt                                                                                                                                                   >  2:1148697/1‑150 (MQ=255)
    tAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATg                                                                                                                                               >  2:1033232/1‑150 (MQ=255)
      gTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCACCCGTTATGGc                                                                                                                                             >  1:1483360/1‑150 (MQ=255)
       ttCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCg                                                                                                                                            >  1:972922/1‑150 (MQ=255)
        tCAGGGGTTTCGGTTAACGGTTCTGTCCCCCGGTTCTCCTCCGTCTCTTTTCTTATTGTGTTGCGCAGCACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCgg                                                                                                                                           >  2:421451/1‑150 (MQ=255)
         cAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGAAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGt                                                                                                                                          <  2:1031568/150‑1 (MQ=255)
           ggggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTg                                                                                                                                        >  1:607548/1‑150 (MQ=255)
             ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGc                                                                                                                                      <  2:929817/150‑1 (MQ=255)
             ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGc                                                                                                                                      <  1:971712/150‑1 (MQ=255)
              gTTTCTGTTTAAGGTTCAGTCTCCCGGGTCTCCTCCTTCTCTTTTCTTATTGTGATCCCCAGAACGCGATTTACAGACATGTAAGTATAGACAGTGTGAATGGGGTCGCCATTTGCTTCGAAGAACTGCCAACCGCCATACCGGTTGGCt                                                                                                                                     <  1:998988/150‑1 (MQ=255)
               tttCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTa                                                                                                                                    <  1:77626/150‑1 (MQ=255)
                ttCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGGAACGAAGAACAGCCAACCGTTTTGGCGGTTGGCTAt                                                                                                                                   >  1:325287/1‑150 (MQ=255)
                 tCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtg                                                                                                                                  <  1:1255829/150‑1 (MQ=255)
                  cGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtgg                                                                                                                                 <  1:1167533/150‑1 (MQ=255)
                   ggTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggt                                                                                                                                >  1:1743605/1‑150 (MQ=255)
                    gTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtg                                                                                                                               >  1:867040/1‑150 (MQ=255)
                    gTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATCTAAGTATAGACAGTGTGACGGGGGTCGCCAATTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtg                                                                                                                               >  1:591560/1‑150 (MQ=255)
                     ttAACGGTTCTGTCACCCGGGTCTCCTCCTTCTCTTTTCTTATTGTGTTCCGCAGAACGCGCTTTACAGACATGAAAGTATAGACAGTGTGACGGGGGGCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtgg                                                                                                                              <  1:348622/150‑1 (MQ=255)
                      tAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCTGAACGCTCTTTACTGACATGTAAGTATAGACAGGGTGACGGGGGTCGCCATTTGGAACGAAGTACAGCCATCCGTTATGGCGGTTGGCTATGGTggg                                                                                                                             <  2:49270/150‑1 (MQ=255)
                      tAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTggg                                                                                                                             <  1:15880/150‑1 (MQ=255)
                          ggTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGaaaa                                                                                                                         <  1:1811775/150‑1 (MQ=255)
                             gcTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGTCGGGGGGCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAAc                                                                                                                      <  2:1743428/149‑1 (MQ=255)
                              cTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACg                                                                                                                     >  2:876606/1‑150 (MQ=255)
                               tGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGc                                                                                                                    >  2:1293958/1‑150 (MQ=255)
                                   aCCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTaaa                                                                                                                >  1:568297/1‑150 (MQ=255)
                                      cGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAAttg                                                                                                             >  2:1495205/1‑150 (MQ=255)
                                        gTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAAttgtt                                                                                                           <  2:1314896/150‑1 (MQ=255)
                                         atctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACTGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAAttgttg                                                                                                          <  2:1466005/149‑1 (MQ=255)
                                          tctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGc                                                                                                         <  2:568297/150‑1 (MQ=255)
                                          tctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGc                                                                                                         <  1:440271/150‑1 (MQ=255)
                                          tctcCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGc                                                                                                         <  1:1404765/150‑1 (MQ=255)
                                          tctcCTCCTTCTCTTTTCTTATTGTGTGGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGc                                                                                                         <  1:862013/150‑1 (MQ=255)
                                            tcctccTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCGTTCCAGACATGTCAGTATAGACCGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCACCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAg                                                                                                       <  1:1125030/150‑1 (MQ=255)
                                            tcctccTTCTCTTTTCTTAGTGTGTTGCGCAGAACGCGCTTTACAGACATGTAACTATAGACACTGTGACTTGGGGCGCCATTAGCACCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAg                                                                                                       <  1:1119038/150‑1 (MQ=255)
                                             cctcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGa                                                                                                      >  1:675542/1‑150 (MQ=255)
                                              ctcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGaa                                                                                                     >  1:1786357/1‑150 (MQ=255)
                                                 ctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGaaaaa                                                                                                  >  2:1034615/1‑150 (MQ=255)
                                                 ctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGaaaaa                                                                                                  <  2:607548/150‑1 (MQ=255)
                                                   tctcTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAg                                                                                                >  1:979808/1‑150 (MQ=255)
                                                     tctTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCa                                                                                              <  1:1148697/150‑1 (MQ=255)
                                                       ttttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTATGTATAGACTGTGTGACGGTGGTAGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTTGCTATGGTGGGAAAAAACTCTAAATTGTTGCAGAAAAAAGCATc                                                                                            <  2:1550302/150‑1 (MQ=255)
                                                       ttttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGCCGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATc                                                                                            <  2:1143289/150‑1 (MQ=255)
                                                       ttttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATc                                                                                            >  2:628165/1‑150 (MQ=255)
                                                         ttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCTACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAg                                                                                          >  1:892206/1‑150 (MQ=255)
                                                                 gtgtTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCg                                                                                  >  2:1071368/1‑150 (MQ=255)
                                                                   gtTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGtt                                                                                >  2:1657345/1‑150 (MQ=255)
                                                                   gtTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGAAACAAAGAACAGCCAACCGTTATGGAGGTTGTCTATTGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGtt                                                                                >  2:342616/1‑150 (MQ=255)
                                                                      gcgcAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCg                                                                             >  2:907573/1‑150 (MQ=255)
                                                                        gcAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCt                                                                           >  1:1716676/1‑150 (MQ=255)
                                                                         cAGAACGCGCTTTACAGACATGTAAGTATAGACCGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCtt                                                                          <  1:1327477/150‑1 (MQ=255)
                                                                         cAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCtt                                                                          <  1:1071368/150‑1 (MQ=255)
                                                                           gAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAAGGAAGAACAGCCAAGCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCtttt                                                                        <  2:1009533/150‑1 (MQ=255)
                                                                            aaCGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCttttt                                                                       <  1:1030002/150‑1 (MQ=255)
                                                                              cgcgCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGc                                                                     >  1:1210479/1‑150 (MQ=255)
                                                                                  cTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGt                                                                 <  1:998090/150‑1 (MQ=255)
                                                                                     tACAGACATGTAAGTATAGACAGTGTGACGGGGGTGGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAAc                                                              <  1:123135/150‑1 (MQ=255)
                                                                                       cAGACATGTATGTATAGTCAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAAGCGTGATGGCGGTTGGCTATGGTGGAAAAAAACGCTAAATTGTTGCAGATAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTATCgg                                                            <  1:1053910/150‑1 (MQ=255)
                                                                                       cAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACgg                                                            >  1:549992/1‑150 (MQ=255)
                                                                                         gACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTc                                                          >  1:1256055/1‑150 (MQ=255)
                                                                                          ataTGTAAGTATTGACAGTGTGACGGGTGTCGCCATTTGAAACGAAGAAAAGCCAACCGTTATGGCTGTTGGCTATGGTTGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGATTTTTGCTTGTAACGGTCg                                                         <  1:200647/148‑1 (MQ=255)
                                                                                          aCATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCg                                                         <  2:1755236/150‑1 (MQ=255)
                                                                                           cATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGACGa                                                        >  1:1096509/1‑150 (MQ=255)
                                                                                            aTGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGaa                                                       >  2:598338/1‑150 (MQ=255)
                                                                                                 aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGGCGAAGATCa                                                  <  1:726382/150‑1 (MQ=255)
                                                                                                  gTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAc                                                 >  1:909506/1‑150 (MQ=255)
                                                                                                  gTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAc                                                 >  1:240565/1‑150 (MQ=255)
                                                                                                   tataGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGGCGAAGATCAcc                                                <  1:1825953/150‑1 (MQ=255)
                                                                                                    aaaGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCg                                               <  1:1197980/148‑1 (MQ=255)
                                                                                                         cAGTGTGACGGGGGGCGCCATTTGCAACGAAGAACAGCCAACCGGTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGcc                                          <  1:1452605/150‑1 (MQ=255)
                                                                                                           gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAATATAACCGCTGCcag                                        >  1:999178/1‑150 (MQ=255)
                                                                                                           gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcag                                        >  2:1558189/1‑150 (MQ=255)
                                                                                                               gACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAgg                                    <  2:831396/150‑1 (MQ=255)
                                                                                                                 cGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAt                                  <  2:867040/150‑1 (MQ=255)
                                                                                                                 cGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAt                                  <  1:1495205/150‑1 (MQ=255)
                                                                                                                    gggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTaa                               <  1:1713846/150‑1 (MQ=255)
                                                                                                                     ggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAg                              >  1:1470798/1‑150 (MQ=255)
                                                                                                                     ggTCGCAATTTGCAACGAAGAACAGCCAACCGTTATGGCGGGTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAg                              <  2:1132540/150‑1 (MQ=255)
                                                                                                                        cGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCcg                           <  2:383410/150‑1 (MQ=255)
                                                                                                                         gCCTTTTGCAACGAATAACTGCCAACCGTTATGGCTTTTGGCTTTGGTGGGTAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCTCTGCCAGCAGGATTAAGCcgc                          <  1:825070/150‑1 (MQ=255)
                                                                                                                           cATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGa                        >  1:70829/1‑150 (MQ=255)
                                                                                                                                 cAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACg                  >  2:191461/1‑150 (MQ=255)
                                                                                                                                  aaCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGt                 <  2:1264345/150‑1 (MQ=255)
                                                                                                                                    cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc               <  2:1273346/150‑1 (MQ=255)
                                                                                                                                    cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTAc               >  1:1003392/1‑150 (MQ=255)
                                                                                                                                      aagaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCTCGAACCACGTACTg             >  1:1471193/1‑150 (MQ=255)
                                                                                                                                      aagaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTg             >  1:1820726/1‑150 (MQ=255)
                                                                                                                                       agaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTgc            <  1:1657345/150‑1 (MQ=255)
                                                                                                                                       agaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTgc            <  1:316111/150‑1 (MQ=255)
                                                                                                                                        gaaCAGCCAACCGCTATGTCGGTTGGCTATGGTGCGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGAGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTgcg           <  2:96687/150‑1 (MQ=255)
                                                                                                                                          ataGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGcgcg         >  1:615452/3‑150 (MQ=255)
                                                                                                                                          ataGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGcgcg         >  2:1786452/3‑150 (MQ=255)
                                                                                                                                           taGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGAAGAAAAAAGCATCAGACAGTGCGTTTCGATTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGa        >  2:1515555/2‑150 (MQ=255)
                                                                                                                                           cAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGa        >  2:836873/1‑150 (MQ=255)
                                                                                                                                           cAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGa        <  1:1034615/150‑1 (MQ=255)
                                                                                                                                           cAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGGAACGGTCGACGCTCACCGCTGCCAGCAGGTTTCAGCCGCGCACCACGTACTGcgcgc        >  2:288307/1‑149 (MQ=255)
                                                                                                                                              ccAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAg     <  2:1081331/150‑1 (MQ=255)
                                                                                                                                              ccAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAg     <  1:1535901/150‑1 (MQ=255)
                                                                                                                                               cAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGAGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTTCTGCGCGAAAgg    >  1:79154/1‑150 (MQ=255)
                                                                                                                                                 aCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag  >  1:1391614/1‑150 (MQ=255)
                                                                                                                                                 aCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag  >  1:1383871/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAG  >  NC_000913/1982351‑1982645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: