Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,926 A→C 9.0% intergenic (+34/+28) tyrP → / ← yecA tyrosine transporter/UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,9260AC 9.0% 319.7 / 20.0 111intergenic (+34/+28)tyrP/yecAtyrosine transporter/UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base A (50/51);  new base C (4/6);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

Only 100 of 114 total aligned reads displayed.

CGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCA  >  NC_000913/1990777‑1991072
                                                                                                                                                     |                                                                                                                                                  
ggggTTACGGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGTTTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGa                                                                                                                                                    <  1:1533972/149‑1 (MQ=255)
cGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGa                                                                                                                                                    >  1:1021291/1‑150 (MQ=255)
 gggTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGaa                                                                                                                                                   <  2:1292025/150‑1 (MQ=255)
      tccGGGTCATAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTTGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                                                                                              <  1:1538/149‑1 (MQ=255)
      aCCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                                                                                              >  2:4843/1‑150 (MQ=255)
       ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACg                                                                                                                                             >  1:1065440/1‑150 (MQ=255)
       ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACg                                                                                                                                             >  1:1575476/1‑150 (MQ=255)
           gTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGa                                                                                                                                         <  1:534005/150‑1 (MQ=255)
            tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                        <  1:31701/150‑1 (MQ=255)
            tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCGGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                        >  2:366335/1‑150 (MQ=255)
                    ggtCGTGCGGCGCTGGTGGGGGAGTTTCTCTGGGGTATTGCTGTGCGTGGCGGGCAATGTTTGATAGCGGCAGGGTTGTTACCAGAAGGGGGGGGATCAGATAGCCTCAAATTCCTTCTTGGGTGTCAGCGTTAATTCTGGCACCCAAta                                                                                                                                <  1:1614133/150‑2 (MQ=255)
                      tcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                              <  1:1064510/150‑4 (MQ=255)
                      gcggccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                              <  1:649022/146‑4 (MQ=255)
                       ggtcCGGCGCTGGTGGTGGTGGTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCGTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaagg                                                                                                                             <  2:475722/149‑5 (MQ=38)
                       cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTgg                                                                                                                             <  1:1483564/150‑1 (MQ=255)
                            ggCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctct                                                                                                                        >  1:1577968/1‑150 (MQ=255)
                            ggCGCTGGTGGTGGGGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTCATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATAAACGCAGACACCCAATTTGGCctct                                                                                                                        <  1:814097/150‑1 (MQ=255)
                              cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                      <  2:638603/150‑1 (MQ=255)
                              cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                      <  2:589759/150‑1 (MQ=255)
                                cTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTACCGCTGCCACCCAATTTGGCCTCTTAAt                                                                                                                    >  1:449143/1‑150 (MQ=255)
                                   gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                 <  2:797182/150‑1 (MQ=255)
                                    tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                <  1:516748/150‑1 (MQ=255)
                                    tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGACCTCTTAATgcag                                                                                                                <  1:656688/150‑1 (MQ=255)
                                    gggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                <  2:1278683/149‑1 (MQ=255)
                                    ggggggggtTGCTCTGTGGAATTGCTGTGATTGCGGTGCAATTTTTTATTGCGGCAGGGTTGTTCCCAGAAGTGGGGGGATCAGATACCCTAAAATACCTTATTGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcagg                                                                                                               <  1:1564752/143‑1 (MQ=255)
                                      gtggtgTTTCTCTGGGGTATTGCTGTGATTGGGGTTCAATTTTTGATTGCGGCAGGGTGGTTACCAGAAGTGGGGTGATCCGATAGCCTCAAATTCCTTATTGGCTGCCTGAATTAACGCTGACACGCAATTTTGCCTCTTAATgcaggc                                                                                                              <  2:1278044/150‑1 (MQ=255)
                                       tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                             >  1:551130/1‑150 (MQ=255)
                                       tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                             >  1:364816/1‑150 (MQ=255)
                                        ggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggcag                                                                                                            >  2:591590/1‑150 (MQ=255)
                                         gtgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagc                                                                                                           <  1:518937/150‑1 (MQ=255)
                                          tgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagca                                                                                                          >  1:446331/1‑150 (MQ=255)
                                           gtTTCTCTGTGGTATCGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAc                                                                                                         <  1:366829/150‑1 (MQ=255)
                                                   gtgGTATTGCTGTCATTGGCGTGCAATTTTTGAATGCGGCAGGGTTGTTACCAGAAGTCGGGTGATCAGATAGCATCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAACCAATTTGGACTCTTAATTCAGTCAGCACTGATTaaa                                                                                                 >  2:316735/1‑150 (MQ=255)
                                                    tgGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGTTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTATGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAt                                                                                                <  1:1122552/150‑1 (MQ=255)
                                                          tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTa                                                                                          <  1:1057083/150‑1 (MQ=255)
                                                            cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                        <  1:989405/150‑1 (MQ=255)
                                                             tgtgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCa                                                                                       <  1:917152/150‑1 (MQ=255)
                                                                    ggCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCCTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGc                                                                                >  1:884469/1‑150 (MQ=255)
                                                                       gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTACTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAGTTCTTACCACTACCGCACg                                                                             >  1:119069/1‑150 (MQ=255)
                                                                         gCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg                                                                           >  1:1434522/1‑150 (MQ=255)
                                                                          cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc                                                                          >  2:82176/1‑150 (MQ=255)
                                                                           aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCa                                                                         <  2:943397/150‑1 (MQ=255)
                                                                            aTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCaa                                                                        >  1:373592/1‑150 (MQ=255)
                                                                             tttttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAg                                                                       >  2:1700708/1‑150 (MQ=255)
                                                                                 tGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATc                                                                   <  2:958343/150‑1 (MQ=255)
                                                                                  gATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCg                                                                  <  1:1518017/150‑1 (MQ=255)
                                                                                     tGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa                                                               <  1:693557/150‑1 (MQ=255)
                                                                                      gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGTTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                              <  1:807281/150‑1 (MQ=255)
                                                                                       cGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGTGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACg                                                             >  2:938677/1‑150 (MQ=255)
                                                                                        ggCAGGGTTGTTACGAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCTGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGc                                                            <  1:439665/150‑1 (MQ=255)
                                                                                           aGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGcccc                                                         <  2:1595180/150‑1 (MQ=255)
                                                                                              gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCgct                                                      >  2:216094/1‑148 (MQ=255)
                                                                                              gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt                                                      >  2:1449904/1‑150 (MQ=255)
                                                                                                 gttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc                                                   >  2:1081317/1‑150 (MQ=255)
                                                                                                   tACCAGAAGTGGGGTGATCAGATAGCCACAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc                                                 >  1:1295384/1‑150 (MQ=255)
                                                                                                    aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                <  2:1405722/150‑1 (MQ=255)
                                                                                                     ccAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                               >  1:424362/1‑150 (MQ=255)
                                                                                                         aaGTGGGGTGATCAGATAGCCTCAAATTCCTTATAGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGc                                           <  2:391933/150‑1 (MQ=255)
                                                                                                          aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTAAAACCACTACCGCACGGGCAAGGATCGTTACTCCCCGGTTACTCTTCTgat                                          >  1:1791454/1‑148 (MQ=255)
                                                                                                          aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCt                                          <  2:446331/150‑1 (MQ=255)
                                                                                                            tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGATTTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTTCGCCCCGGTTTCGGGTCTGCtgt                                        >  2:700735/1‑148 (MQ=255)
                                                                                                            tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                        <  2:1522215/150‑1 (MQ=255)
                                                                                                            tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                        >  1:1267003/1‑150 (MQ=255)
                                                                                                             ggggTGCTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                       <  1:1396143/150‑1 (MQ=255)
                                                                                                             ggggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                       <  1:1700708/150‑1 (MQ=255)
                                                                                                             ggggTGAGCAGATAGCCTCATATTCCTTATTGGGTGCCAGAATTAACGCGGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                       <  1:1747577/150‑1 (MQ=255)
                                                                                                               ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt                                     >  1:1752002/1‑150 (MQ=255)
                                                                                                                gTGATCAGATAGCCTCTAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTTATGGAGGCAGCACTGCTTAAATTTCTTACCACTACGGCACGGGCAAGGATCGTTTCGCCCCGGGTTCTCTTCTGCTTTGATc                                    <  2:1761099/150‑1 (MQ=255)
                                                                                                                 tGATCATATAGCCTCAAATTCCTTATTTGGTGCAAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                   <  2:1531917/150‑1 (MQ=255)
                                                                                                                 tGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                   <  1:366335/150‑1 (MQ=255)
                                                                                                                   aTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt                                 >  1:460438/1‑150 (MQ=255)
                                                                                                                    tCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGtt                                >  1:1392287/1‑150 (MQ=255)
                                                                                                                     cAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTg                               >  2:1525094/1‑150 (MQ=255)
                                                                                                                      aGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGc                              <  2:1049296/150‑1 (MQ=255)
                                                                                                                           gCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAAc                         <  2:1021291/150‑1 (MQ=255)
                                                                                                                             cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg                       <  1:1701834/150‑1 (MQ=255)
                                                                                                                              tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                      >  1:526154/1‑150 (MQ=255)
                                                                                                                              tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                      <  1:4843/150‑1 (MQ=255)
                                                                                                                               cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCt                     >  2:417488/1‑150 (MQ=255)
                                                                                                                                  aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCGCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                  <  2:1577968/150‑1 (MQ=255)
                                                                                                                                  aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                  <  2:1065440/150‑1 (MQ=255)
                                                                                                                                   ggccaaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTTAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCttttt                 >  1:328686/7‑150 (MQ=38)
                                                                                                                                     ccTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGGACAGCTTTTTcc               <  1:804615/150‑1 (MQ=255)
                                                                                                                                      cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt              >  2:1733832/1‑150 (MQ=255)
                                                                                                                                      cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt              >  1:1366878/1‑150 (MQ=255)
                                                                                                                                      cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt              <  1:342353/150‑1 (MQ=255)
                                                                                                                                        tATTGGGTGCCAGAATTAACGCTGACACCCAAATTGTCCTCTTAATGCAGGCAGCACTGCTTAAAATTCTTAACACTACCGCACGGGCAAGGATCGTTACGCCAAGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGc            >  2:739219/1‑150 (MQ=255)
                                                                                                                                         tttGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAATGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg           <  1:453864/149‑1 (MQ=255)
                                                                                                                                         aTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg           >  2:605615/1‑150 (MQ=255)
                                                                                                                                         aTTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg           <  1:969636/150‑1 (MQ=255)
                                                                                                                                            gggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGAt        >  1:786574/1‑150 (MQ=255)
                                                                                                                                             ggTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg       >  1:1449368/1‑150 (MQ=255)
                                                                                                                                             ggTGCCAGAATTAACGCTGACACCCATTGTGGCCTCTTAATGCAGGCCGCACTGCTTAAATTTCTTCCCACTACCGCCCGGGCAAGGATCGTTACGCCCCGGGTTCTCATCTGCTTAGATCGCTTGCTGCACCGCTGTTTCCTGCGAATg       >  1:175496/1‑150 (MQ=255)
                                                                                                                                             ggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg       >  2:1374435/1‑150 (MQ=255)
                                                                                                                                             ggTGCCAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGTCAGCACTGCTTAAATTTCTTAACACTACCTCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg       <  2:1014670/150‑1 (MQ=255)
                                                                                                                                               tGCCAGAATTCACGCGGACACCCAATTTGGCCTCTTAAGGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAGCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg     <  1:77327/150‑1 (MQ=255)
                                                                                                                                               tGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg     >  1:1776714/1‑150 (MQ=255)
                                                                                                                                                gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc    >  1:730373/1‑150 (MQ=255)
                                                                                                                                                 ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGcc   >  1:1590674/1‑150 (MQ=255)
                                                                                                                                                  cAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCa  >  1:841057/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCA  >  NC_000913/1990777‑1991072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: