Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,932 C→T 9.0% intergenic (+40/+22) tyrP → / ← yecA tyrosine transporter/UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,9320CT 9.0% 327.0 / 14.7 110intergenic (+40/+22)tyrP/yecAtyrosine transporter/UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base C (50/50);  new base T (4/6);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 112 total aligned reads displayed.

ACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990783‑1991080
                                                                                                                                                     |                                                                                                                                                    
tccGGGTCATAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTTGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                                                                                                      <  1:1538/149‑1 (MQ=255)
aCCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                                                                                                      >  2:4843/1‑150 (MQ=255)
 ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACg                                                                                                                                                     >  1:1065440/1‑150 (MQ=255)
     gTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGa                                                                                                                                                 <  1:534005/150‑1 (MQ=255)
      tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                <  1:31701/150‑1 (MQ=255)
          aGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCAccc                                                                                                                                            <  1:852174/150‑1 (MQ=255)
              ggtCGTGCGGCGCTGGTGGGGGAGTTTCTCTGGGGTATTGCTGTGCGTGGCGGGCAATGTTTGATAGCGGCAGGGTTGTTACCAGAAGGGGGGGGATCAGATAGCCTCAAATTCCTTCTTGGGTGTCAGCGTTAATTCTGGCACCCAAta                                                                                                                                        <  1:1614133/150‑2 (MQ=255)
               gtcgtcCGGCGCTGGTGGTCGTGTTTCTCTGTGGTTTTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaa                                                                                                                                       <  1:1643518/150‑3 (MQ=255)
                tcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:1064510/150‑4 (MQ=255)
                gcggccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:649022/146‑4 (MQ=255)
                 ggtcCGGCGCTGGTGGTGGTGGTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCGTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaagg                                                                                                                                     <  2:475722/149‑5 (MQ=38)
                 cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTgg                                                                                                                                     <  1:1483564/150‑1 (MQ=255)
                      ggCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctct                                                                                                                                >  1:1577968/1‑150 (MQ=255)
                      ggCGCTGGTGGTGGGGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTCATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATAAACGCAGACACCCAATTTGGCctct                                                                                                                                <  1:814097/150‑1 (MQ=255)
                        cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                              <  2:589759/150‑1 (MQ=255)
                        cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                              <  2:638603/150‑1 (MQ=255)
                          cTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTACCGCTGCCACCCAATTTGGCCTCTTAAt                                                                                                                            >  1:449143/1‑150 (MQ=255)
                             gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         <  2:797182/150‑1 (MQ=255)
                             gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         >  1:1304079/1‑150 (MQ=255)
                              tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  1:516748/150‑1 (MQ=255)
                              tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGACCTCTTAATgcag                                                                                                                        <  1:656688/150‑1 (MQ=255)
                              gggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  2:1278683/149‑1 (MQ=255)
                              ggggggggtTGCTCTGTGGAATTGCTGTGATTGCGGTGCAATTTTTTATTGCGGCAGGGTTGTTCCCAGAAGTGGGGGGATCAGATACCCTAAAATACCTTATTGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcagg                                                                                                                       <  1:1564752/143‑1 (MQ=255)
                                gtggtgTTTCTCTGGGGTATTGCTGTGATTGGGGTTCAATTTTTGATTGCGGCAGGGTGGTTACCAGAAGTGGGGTGATCCGATAGCCTCAAATTCCTTATTGGCTGCCTGAATTAACGCTGACACGCAATTTTGCCTCTTAATgcaggc                                                                                                                      <  2:1278044/150‑1 (MQ=255)
                                 tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:364816/1‑150 (MQ=255)
                                 tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:551130/1‑150 (MQ=255)
                                  ggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggcag                                                                                                                    >  2:591590/1‑150 (MQ=255)
                                   gtgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagc                                                                                                                   <  1:518937/150‑1 (MQ=255)
                                    tgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagca                                                                                                                  >  1:446331/1‑150 (MQ=255)
                                     gtTTCTCTGTGGTATCGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAc                                                                                                                 <  1:366829/150‑1 (MQ=255)
                                             gtgGTATTGCTGTCATTGGCGTGCAATTTTTGAATGCGGCAGGGTTGTTACCAGAAGTCGGGTGATCAGATAGCATCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAACCAATTTGGACTCTTAATTCAGTCAGCACTGATTaaa                                                                                                         >  2:316735/1‑150 (MQ=255)
                                              tgGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGTTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTATGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAt                                                                                                        <  1:1122552/150‑1 (MQ=255)
                                                   ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                                                                                                   <  1:42173/150‑1 (MQ=255)
                                                    tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTa                                                                                                  <  1:1057083/150‑1 (MQ=255)
                                                      cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                                <  1:989405/150‑1 (MQ=255)
                                                       tgtgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCa                                                                                               <  1:917152/150‑1 (MQ=255)
                                                                 gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTACTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAGTTCTTACCACTACCGCACg                                                                                     >  1:119069/1‑150 (MQ=255)
                                                                   gCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg                                                                                   >  1:1434522/1‑150 (MQ=255)
                                                                    cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc                                                                                  >  2:82176/1‑150 (MQ=255)
                                                                     aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCa                                                                                 <  2:943397/150‑1 (MQ=255)
                                                                       tttttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAg                                                                               >  2:1700708/1‑150 (MQ=255)
                                                                            gATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCg                                                                          <  1:1518017/150‑1 (MQ=255)
                                                                               tGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa                                                                       <  1:693557/150‑1 (MQ=255)
                                                                                gCGGCAGGGTTGTTACCAGAATTGGGGTGATCAGATAGCCTCACATTGCTTATTGGGTGGCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGGACGGGCAAGGAGCGTTAc                                                                      <  2:1710746/150‑1 (MQ=255)
                                                                                gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGTTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      <  1:807281/150‑1 (MQ=255)
                                                                                gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      >  1:511375/1‑150 (MQ=255)
                                                                                 cGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGTGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACg                                                                     >  2:938677/1‑150 (MQ=255)
                                                                                  ggCAGGGTTGTTACGAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCTGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGc                                                                    <  1:439665/150‑1 (MQ=255)
                                                                                     aGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGcccc                                                                 <  2:1595180/150‑1 (MQ=255)
                                                                                        gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCgct                                                              >  2:216094/1‑148 (MQ=255)
                                                                                        gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt                                                              >  2:1449904/1‑150 (MQ=255)
                                                                                             tACCAGAAGTGGGGTGATCAGATAGCCACAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc                                                         >  1:1295384/1‑150 (MQ=255)
                                                                                              aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACCCCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        >  2:1055966/1‑150 (MQ=255)
                                                                                              aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        <  2:1405722/150‑1 (MQ=255)
                                                                                               ccAGAAGTGGGGTGTTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  2:422816/1‑150 (MQ=255)
                                                                                               ccAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  1:424362/1‑150 (MQ=255)
                                                                                                   aaGTGGGGTGATCAGATAGCCTCAAATTCCTTATAGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGc                                                   <  2:391933/150‑1 (MQ=255)
                                                                                                    aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTAAAACCACTACCGCACGGGCAAGGATCGTTACTCCCCGGTTACTCTTCTgat                                                  >  1:1791454/1‑148 (MQ=255)
                                                                                                    aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCt                                                  <  2:446331/150‑1 (MQ=255)
                                                                                                      tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGATTTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTTCGCCCCGGTTTCGGGTCTGCtgt                                                >  2:700735/1‑148 (MQ=255)
                                                                                                      tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                <  2:1522215/150‑1 (MQ=255)
                                                                                                      tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                >  1:1267003/1‑150 (MQ=255)
                                                                                                       ggggTGCTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1396143/150‑1 (MQ=255)
                                                                                                       ggggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1700708/150‑1 (MQ=255)
                                                                                                       ggggTGAGCAGATAGCCTCATATTCCTTATTGGGTGCCAGAATTAACGCGGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1747577/150‑1 (MQ=255)
                                                                                                         ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt                                             >  1:1752002/1‑150 (MQ=255)
                                                                                                          gTGATCAGATAGCCTCTAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTTATGGAGGCAGCACTGCTTAAATTTCTTACCACTACGGCACGGGCAAGGATCGTTTCGCCCCGGGTTCTCTTCTGCTTTGATc                                            <  2:1761099/150‑1 (MQ=255)
                                                                                                           tGATCATATAGCCTCAAATTCCTTATTTGGTGCAAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  2:1531917/150‑1 (MQ=255)
                                                                                                           tGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  1:366335/150‑1 (MQ=255)
                                                                                                             aTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt                                         >  1:460438/1‑150 (MQ=255)
                                                                                                                aGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGc                                      <  2:1049296/150‑1 (MQ=255)
                                                                                                                     gCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAAc                                 <  2:1021291/150‑1 (MQ=255)
                                                                                                                       cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg                               <  1:1701834/150‑1 (MQ=255)
                                                                                                                       cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTGTGATCGGTTGCTGAACAg                               >  1:1532469/1‑150 (MQ=255)
                                                                                                                        tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                              >  1:526154/1‑150 (MQ=255)
                                                                                                                         cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCt                             >  2:417488/1‑150 (MQ=255)
                                                                                                                            aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCGCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1577968/150‑1 (MQ=255)
                                                                                                                            aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1065440/150‑1 (MQ=255)
                                                                                                                             ggccaaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTTAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCttttt                         >  1:328686/7‑150 (MQ=38)
                                                                                                                             ggccaaaTTGGGTGTCAGCGTTAATTCAGGCACCCAATTTGGCGTCTTAATGCAGGCAGCACTGCTGAACTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCGTTGATCGGTTGCTGAACAGCttttt                         >  1:292681/7‑150 (MQ=255)
                                                                                                                               ccTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGGACAGCTTTTTcc                       <  1:804615/150‑1 (MQ=255)
                                                                                                                                cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  1:1366878/1‑150 (MQ=255)
                                                                                                                                cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  2:1733832/1‑150 (MQ=255)
                                                                                                                                cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      <  1:342353/150‑1 (MQ=255)
                                                                                                                                  tATTGGGTGCCAGAATTAACGCTGACACCCAAATTGTCCTCTTAATGCAGGCAGCACTGCTTAAAATTCTTAACACTACCGCACGGGCAAGGATCGTTACGCCAAGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGc                    >  2:739219/1‑150 (MQ=255)
                                                                                                                                   tttGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAATGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   <  1:453864/149‑1 (MQ=255)
                                                                                                                                   aTTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   <  1:969636/150‑1 (MQ=255)
                                                                                                                                      gggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGAt                >  1:786574/1‑150 (MQ=255)
                                                                                                                                       ggTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               >  1:1449368/1‑150 (MQ=255)
                                                                                                                                       ggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               >  2:1374435/1‑150 (MQ=255)
                                                                                                                                       ggTGCCAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGTCAGCACTGCTTAAATTTCTTAACACTACCTCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               <  2:1014670/150‑1 (MQ=255)
                                                                                                                                         tGCCAGAATTCACGCGGACACCCAATTTGGCCTCTTAAGGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAGCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             <  1:77327/150‑1 (MQ=255)
                                                                                                                                         tGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             >  1:1776714/1‑150 (MQ=255)
                                                                                                                                          gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  1:730373/1‑150 (MQ=255)
                                                                                                                                          gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCATAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTATGATCGGTTGCTGAACAGCTTTATCCTGCGGATgcgc            >  2:1406890/1‑150 (MQ=255)
                                                                                                                                          gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAATGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  2:65715/1‑150 (MQ=255)
                                                                                                                                           ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGcc           >  1:1590674/1‑150 (MQ=255)
                                                                                                                                            cAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCa          >  1:841057/1‑150 (MQ=255)
                                                                                                                                                   aaCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGATTAAATTTCTTACAACTACCGCACGGGCAAGGATCGTTACGCCCCGGATTATATTATGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCCGTa   >  2:142070/1‑150 (MQ=255)
                                                                                                                                                    aCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTAGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAc  >  1:379144/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990783‑1991080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: