Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,933 G→T 8.5% intergenic (+41/+21) tyrP → / ← yecA tyrosine transporter/UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,9330GT 8.5% 290.4 / 13.5 108intergenic (+41/+21)tyrP/yecAtyrosine transporter/UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base G (48/50);  new base T (4/6);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 110 total aligned reads displayed.

CCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990784‑1991080
                                                                                                                                                     |                                                                                                                                                   
ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACg                                                                                                                                                     >  1:1065440/1‑150 (MQ=255)
ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACg                                                                                                                                                     >  1:1575476/1‑150 (MQ=255)
    gTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGa                                                                                                                                                 <  1:534005/150‑1 (MQ=255)
     tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                <  1:31701/150‑1 (MQ=255)
     tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCGGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                >  2:366335/1‑150 (MQ=255)
         aGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCAccc                                                                                                                                            <  1:852174/150‑1 (MQ=255)
             ggtCGTGCGGCGCTGGTGGGGGAGTTTCTCTGGGGTATTGCTGTGCGTGGCGGGCAATGTTTGATAGCGGCAGGGTTGTTACCAGAAGGGGGGGGATCAGATAGCCTCAAATTCCTTCTTGGGTGTCAGCGTTAATTCTGGCACCCAAta                                                                                                                                        <  1:1614133/150‑2 (MQ=255)
               tcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:1064510/150‑4 (MQ=255)
               gcggccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:649022/146‑4 (MQ=255)
                ggtcCGGCGCTGGTGGTGGTGGTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCGTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaagg                                                                                                                                     <  2:475722/149‑5 (MQ=38)
                cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTgg                                                                                                                                     <  1:1483564/150‑1 (MQ=255)
                     ggCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctct                                                                                                                                >  1:1577968/1‑150 (MQ=255)
                     ggCGCTGGTGGTGGGGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTCATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATAAACGCAGACACCCAATTTGGCctct                                                                                                                                <  1:814097/150‑1 (MQ=255)
                       cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                              <  2:638603/150‑1 (MQ=255)
                         cTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTACCGCTGCCACCCAATTTGGCCTCTTAAt                                                                                                                            >  1:449143/1‑150 (MQ=255)
                            gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         >  1:1304079/1‑150 (MQ=255)
                            gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         <  2:797182/150‑1 (MQ=255)
                             tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  1:516748/150‑1 (MQ=255)
                             tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGACCTCTTAATgcag                                                                                                                        <  1:656688/150‑1 (MQ=255)
                             gggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  2:1278683/149‑1 (MQ=255)
                             ggggggggtTGCTCTGTGGAATTGCTGTGATTGCGGTGCAATTTTTTATTGCGGCAGGGTTGTTCCCAGAAGTGGGGGGATCAGATACCCTAAAATACCTTATTGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcagg                                                                                                                       <  1:1564752/143‑1 (MQ=255)
                               gtggtgTTTCTCTGGGGTATTGCTGTGATTGGGGTTCAATTTTTGATTGCGGCAGGGTGGTTACCAGAAGTGGGGTGATCCGATAGCCTCAAATTCCTTATTGGCTGCCTGAATTAACGCTGACACGCAATTTTGCCTCTTAATgcaggc                                                                                                                      <  2:1278044/150‑1 (MQ=255)
                                tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:364816/1‑150 (MQ=255)
                                tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:551130/1‑150 (MQ=255)
                                 ggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggcag                                                                                                                    >  2:591590/1‑150 (MQ=255)
                                  gtgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagc                                                                                                                   <  1:518937/150‑1 (MQ=255)
                                   tgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagca                                                                                                                  >  1:446331/1‑150 (MQ=255)
                                    gtTTCTCTGTGGTATCGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAc                                                                                                                 <  1:366829/150‑1 (MQ=255)
                                            gtgGTATTGCTGTCATTGGCGTGCAATTTTTGAATGCGGCAGGGTTGTTACCAGAAGTCGGGTGATCAGATAGCATCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAACCAATTTGGACTCTTAATTCAGTCAGCACTGATTaaa                                                                                                         >  2:316735/1‑150 (MQ=255)
                                             tgGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGTTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTATGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAt                                                                                                        <  1:1122552/150‑1 (MQ=255)
                                                  ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                                                                                                   <  1:42173/150‑1 (MQ=255)
                                                   tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTa                                                                                                  <  1:1057083/150‑1 (MQ=255)
                                                     cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                                <  1:989405/150‑1 (MQ=255)
                                                             ggCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCCTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGc                                                                                        >  1:884469/1‑150 (MQ=255)
                                                                gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTACTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAGTTCTTACCACTACCGCACg                                                                                     >  1:119069/1‑150 (MQ=255)
                                                                  gCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg                                                                                   >  1:1434522/1‑150 (MQ=255)
                                                                   cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc                                                                                  >  2:82176/1‑150 (MQ=255)
                                                                    aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCa                                                                                 <  2:943397/150‑1 (MQ=255)
                                                                     aTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCaa                                                                                >  1:373592/1‑150 (MQ=255)
                                                                      tttttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAg                                                                               >  2:1700708/1‑150 (MQ=255)
                                                                           gATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCg                                                                          <  1:1518017/150‑1 (MQ=255)
                                                                              tGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa                                                                       <  1:693557/150‑1 (MQ=255)
                                                                               gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGTTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      <  1:807281/150‑1 (MQ=255)
                                                                               gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      >  1:511375/1‑150 (MQ=255)
                                                                                cGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGTGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACg                                                                     >  2:938677/1‑150 (MQ=255)
                                                                                 ggCAGGGTTGTTACGAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCTGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGc                                                                    <  1:439665/150‑1 (MQ=255)
                                                                                    aGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGcccc                                                                 <  2:1595180/150‑1 (MQ=255)
                                                                                       gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCgct                                                              >  2:216094/1‑148 (MQ=255)
                                                                                       gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt                                                              >  2:1449904/1‑150 (MQ=255)
                                                                                          gttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc                                                           >  2:1081317/1‑150 (MQ=255)
                                                                                            tACCAGAAGTGGGGTGATCAGATAGCCACAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc                                                         >  1:1295384/1‑150 (MQ=255)
                                                                                             aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACCCCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        >  2:1055966/1‑150 (MQ=255)
                                                                                             aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        <  2:1405722/150‑1 (MQ=255)
                                                                                              ccAGAAGTGGGGTGTTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  2:422816/1‑150 (MQ=255)
                                                                                              ccAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  1:424362/1‑150 (MQ=255)
                                                                                                  aaGTGGGGTGATCAGATAGCCTCAAATTCCTTATAGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGc                                                   <  2:391933/150‑1 (MQ=255)
                                                                                                   aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTAAAACCACTACCGCACGGGCAAGGATCGTTACTCCCCGGTTACTCTTCTgat                                                  >  1:1791454/1‑148 (MQ=255)
                                                                                                   aGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCt                                                  <  2:446331/150‑1 (MQ=255)
                                                                                                     tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGATTTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTTCGCCCCGGTTTCGGGTCTGCtgt                                                >  2:700735/1‑148 (MQ=255)
                                                                                                     tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                <  2:1522215/150‑1 (MQ=255)
                                                                                                     tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                >  1:1267003/1‑150 (MQ=255)
                                                                                                      ggggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1700708/150‑1 (MQ=255)
                                                                                                      ggggTGAGCAGATAGCCTCATATTCCTTATTGGGTGCCAGAATTAACGCGGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1747577/150‑1 (MQ=255)
                                                                                                        ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt                                             >  1:1752002/1‑150 (MQ=255)
                                                                                                         gTGATCAGATAGCCTCTAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTTATGGAGGCAGCACTGCTTAAATTTCTTACCACTACGGCACGGGCAAGGATCGTTTCGCCCCGGGTTCTCTTCTGCTTTGATc                                            <  2:1761099/150‑1 (MQ=255)
                                                                                                          tGATCATATAGCCTCAAATTCCTTATTTGGTGCAAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  2:1531917/150‑1 (MQ=255)
                                                                                                          tGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  1:366335/150‑1 (MQ=255)
                                                                                                            aTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt                                         >  1:460438/1‑150 (MQ=255)
                                                                                                             tCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGtt                                        >  1:1392287/1‑150 (MQ=255)
                                                                                                              cAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTg                                       >  2:1525094/1‑150 (MQ=255)
                                                                                                               aGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGc                                      <  2:1049296/150‑1 (MQ=255)
                                                                                                                    gCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAAc                                 <  2:1021291/150‑1 (MQ=255)
                                                                                                                      cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg                               <  1:1701834/150‑1 (MQ=255)
                                                                                                                      cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTGTGATCGGTTGCTGAACAg                               >  1:1532469/1‑150 (MQ=255)
                                                                                                                       tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                              >  1:526154/1‑150 (MQ=255)
                                                                                                                       tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                              <  1:4843/150‑1 (MQ=255)
                                                                                                                        cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCt                             >  2:417488/1‑150 (MQ=255)
                                                                                                                           aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCGCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1577968/150‑1 (MQ=255)
                                                                                                                           aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1065440/150‑1 (MQ=255)
                                                                                                                            ggccaaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTTAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCttttt                         >  1:328686/7‑150 (MQ=38)
                                                                                                                            ggccaaaTTGGGTGTCAGCGTTAATTCAGGCACCCAATTTGGCGTCTTAATGCAGGCAGCACTGCTGAACTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCGTTGATCGGTTGCTGAACAGCttttt                         >  1:292681/7‑150 (MQ=255)
                                                                                                                              ccTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGGACAGCTTTTTcc                       <  1:804615/150‑1 (MQ=255)
                                                                                                                               cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  1:1366878/1‑150 (MQ=255)
                                                                                                                               cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  2:1733832/1‑150 (MQ=255)
                                                                                                                                 tATTGGGTGCCAGAATTAACGCTGACACCCAAATTGTCCTCTTAATGCAGGCAGCACTGCTTAAAATTCTTAACACTACCGCACGGGCAAGGATCGTTACGCCAAGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGc                    >  2:739219/1‑150 (MQ=255)
                                                                                                                                  aTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   >  2:605615/1‑150 (MQ=255)
                                                                                                                                  aTTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   <  1:969636/150‑1 (MQ=255)
                                                                                                                                     gggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGAt                >  1:786574/1‑150 (MQ=255)
                                                                                                                                      ggTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               >  1:1449368/1‑150 (MQ=255)
                                                                                                                                      ggTGCCAGAATTAACGCTGACACCCATTGTGGCCTCTTAATGCAGGCCGCACTGCTTAAATTTCTTCCCACTACCGCCCGGGCAAGGATCGTTACGCCCCGGGTTCTCATCTGCTTAGATCGCTTGCTGCACCGCTGTTTCCTGCGAATg               >  1:175496/1‑150 (MQ=255)
                                                                                                                                      ggTGCCAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGTCAGCACTGCTTAAATTTCTTAACACTACCTCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               <  2:1014670/150‑1 (MQ=255)
                                                                                                                                        tGCCAGAATTCACGCGGACACCCAATTTGGCCTCTTAAGGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAGCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             <  1:77327/150‑1 (MQ=255)
                                                                                                                                        tGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             >  1:1776714/1‑150 (MQ=255)
                                                                                                                                         gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  1:730373/1‑150 (MQ=255)
                                                                                                                                         gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCATAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTATGATCGGTTGCTGAACAGCTTTATCCTGCGGATgcgc            >  2:1406890/1‑150 (MQ=255)
                                                                                                                                         gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAATGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  2:65715/1‑150 (MQ=255)
                                                                                                                                          ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGcc           >  1:1590674/1‑150 (MQ=255)
                                                                                                                                           cAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCa          >  1:841057/1‑150 (MQ=255)
                                                                                                                                                  aaCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGATTAAATTTCTTACAACTACCGCACGGGCAAGGATCGTTACGCCCCGGATTATATTATGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCCGTa   >  2:142070/1‑150 (MQ=255)
                                                                                                                                                   aCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTAGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAc  >  1:379144/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990784‑1991080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: