Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,937 A→G 9.6% intergenic (+45/+17) tyrP → / ← yecA tyrosine transporter/UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,9370AG 9.6% 280.2 / 17.3 105intergenic (+45/+17)tyrP/yecAtyrosine transporter/UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base A (46/48);  new base G (4/6);  total (51/54)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 108 total aligned reads displayed.

GTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990788‑1991080
                                                                                                                                                     |                                                                                                                                               
gTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGa                                                                                                                                                 <  1:534005/150‑1 (MQ=255)
 tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                <  1:31701/150‑1 (MQ=255)
 tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCGGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                >  2:366335/1‑150 (MQ=255)
     aGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCAccc                                                                                                                                            <  1:852174/150‑1 (MQ=255)
         ggtCGTGCGGCGCTGGTGGGGGAGTTTCTCTGGGGTATTGCTGTGCGTGGCGGGCAATGTTTGATAGCGGCAGGGTTGTTACCAGAAGGGGGGGGATCAGATAGCCTCAAATTCCTTCTTGGGTGTCAGCGTTAATTCTGGCACCCAAta                                                                                                                                        <  1:1614133/150‑2 (MQ=255)
          gtcgtcCGGCGCTGGTGGTCGTGTTTCTCTGTGGTTTTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaa                                                                                                                                       <  1:1643518/150‑3 (MQ=255)
           tcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:1064510/150‑4 (MQ=255)
           gcggccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                      <  1:649022/146‑4 (MQ=255)
            ggtcCGGCGCTGGTGGTGGTGGTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCGTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaagg                                                                                                                                     <  2:475722/149‑5 (MQ=38)
            cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTgg                                                                                                                                     <  1:1483564/150‑1 (MQ=255)
                 ggCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctct                                                                                                                                >  1:1577968/1‑150 (MQ=255)
                 ggCGCTGGTGGTGGGGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTCATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATAAACGCAGACACCCAATTTGGCctct                                                                                                                                <  1:814097/150‑1 (MQ=255)
                   cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                              <  2:638603/150‑1 (MQ=255)
                   cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                                                                                              <  2:589759/150‑1 (MQ=255)
                        gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         <  2:797182/150‑1 (MQ=255)
                        gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                         >  1:1304079/1‑150 (MQ=255)
                         tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  1:516748/150‑1 (MQ=255)
                         tggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGACCTCTTAATgcag                                                                                                                        <  1:656688/150‑1 (MQ=255)
                         gggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                                                                                                        <  2:1278683/149‑1 (MQ=255)
                         ggggggggtTGCTCTGTGGAATTGCTGTGATTGCGGTGCAATTTTTTATTGCGGCAGGGTTGTTCCCAGAAGTGGGGGGATCAGATACCCTAAAATACCTTATTGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcagg                                                                                                                       <  1:1564752/143‑1 (MQ=255)
                           gtggtgTTTCTCTGGGGTATTGCTGTGATTGGGGTTCAATTTTTGATTGCGGCAGGGTGGTTACCAGAAGTGGGGTGATCCGATAGCCTCAAATTCCTTATTGGCTGCCTGAATTAACGCTGACACGCAATTTTGCCTCTTAATgcaggc                                                                                                                      <  2:1278044/150‑1 (MQ=255)
                            tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:551130/1‑150 (MQ=255)
                            tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                                                                                                     >  1:364816/1‑150 (MQ=255)
                             ggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggcag                                                                                                                    >  2:591590/1‑150 (MQ=255)
                              gtgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagc                                                                                                                   <  1:518937/150‑1 (MQ=255)
                               tgtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGgcagca                                                                                                                  >  1:446331/1‑150 (MQ=255)
                                gtTTCTCTGTGGTATCGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAc                                                                                                                 <  1:366829/150‑1 (MQ=255)
                                        gtgGTATTGCTGTCATTGGCGTGCAATTTTTGAATGCGGCAGGGTTGTTACCAGAAGTCGGGTGATCAGATAGCATCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAACCAATTTGGACTCTTAATTCAGTCAGCACTGATTaaa                                                                                                         >  2:316735/1‑150 (MQ=255)
                                         tgGTATTGCTGTGATTGTCGTGCAATTTTTGATTGCGGCAGGTTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTATGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAt                                                                                                        <  1:1122552/150‑1 (MQ=255)
                                              ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                                                                                                   <  1:673901/150‑1 (MQ=255)
                                              ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                                                                                                   <  1:42173/150‑1 (MQ=255)
                                               tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTa                                                                                                  <  1:1057083/150‑1 (MQ=255)
                                                 cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                                <  1:989405/150‑1 (MQ=255)
                                                  tgtgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCa                                                                                               <  1:917152/150‑1 (MQ=255)
                                                         ggCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCCTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGc                                                                                        >  1:884469/1‑150 (MQ=255)
                                                            gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTACTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAGTTCTTACCACTACCGCACg                                                                                     >  1:119069/1‑150 (MQ=255)
                                                              gCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg                                                                                   >  1:1434522/1‑150 (MQ=255)
                                                               cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc                                                                                  >  2:82176/1‑150 (MQ=255)
                                                                aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCa                                                                                 <  2:943397/150‑1 (MQ=255)
                                                                 aTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCaa                                                                                >  1:373592/1‑150 (MQ=255)
                                                                  tttttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAg                                                                               >  2:1700708/1‑150 (MQ=255)
                                                                           gCGGCAGGGTTGTTACCAGAATTGGGGTGATCAGATAGCCTCACATTGCTTATTGGGTGGCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGGACGGGCAAGGAGCGTTAc                                                                      <  2:1710746/150‑1 (MQ=255)
                                                                           gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGTTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      <  1:807281/150‑1 (MQ=255)
                                                                           gCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTAc                                                                      >  1:511375/1‑150 (MQ=255)
                                                                            cGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGTGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACg                                                                     >  2:938677/1‑150 (MQ=255)
                                                                             ggCAGGGTTGTTACGAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCTGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGc                                                                    <  1:439665/150‑1 (MQ=255)
                                                                                aGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGcccc                                                                 <  2:1595180/150‑1 (MQ=255)
                                                                                   gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCgct                                                              >  2:216094/1‑148 (MQ=255)
                                                                                   gttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt                                                              >  2:1449904/1‑150 (MQ=255)
                                                                                      gttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc                                                           >  2:1081317/1‑150 (MQ=255)
                                                                                        tACCAGAAGTGGGGTGATCAGATAGCCACAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc                                                         >  1:1295384/1‑150 (MQ=255)
                                                                                         aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACCCCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        >  2:1055966/1‑150 (MQ=255)
                                                                                         aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                        <  2:1405722/150‑1 (MQ=255)
                                                                                          ccAGAAGTGGGGTGTTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  2:422816/1‑150 (MQ=255)
                                                                                          ccAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                       >  1:424362/1‑150 (MQ=255)
                                                                                              aaGTGGGGTGATCAGATAGCCTCAAATTCCTTATAGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGc                                                   <  2:391933/150‑1 (MQ=255)
                                                                                                 tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGATTTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTTCGCCCCGGTTTCGGGTCTGCtgt                                                >  2:700735/1‑148 (MQ=255)
                                                                                                 tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                <  2:1522215/150‑1 (MQ=255)
                                                                                                 tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                >  1:1267003/1‑150 (MQ=255)
                                                                                                  ggggTGCTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1396143/150‑1 (MQ=255)
                                                                                                  ggggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1700708/150‑1 (MQ=255)
                                                                                                  ggggTGAGCAGATAGCCTCATATTCCTTATTGGGTGCCAGAATTAACGCGGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTg                                               <  1:1747577/150‑1 (MQ=255)
                                                                                                    ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt                                             >  1:1752002/1‑150 (MQ=255)
                                                                                                     gTGATCAGATAGCCTCTAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTTATGGAGGCAGCACTGCTTAAATTTCTTACCACTACGGCACGGGCAAGGATCGTTTCGCCCCGGGTTCTCTTCTGCTTTGATc                                            <  2:1761099/150‑1 (MQ=255)
                                                                                                      tGATCATATAGCCTCAAATTCCTTATTTGGTGCAAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGGCAGCACTTCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  2:1531917/150‑1 (MQ=255)
                                                                                                      tGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg                                           <  1:366335/150‑1 (MQ=255)
                                                                                                        aTCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt                                         >  1:460438/1‑150 (MQ=255)
                                                                                                         tCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGtt                                        >  1:1392287/1‑150 (MQ=255)
                                                                                                           aGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGc                                      <  2:1049296/150‑1 (MQ=255)
                                                                                                                  cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg                               <  1:1701834/150‑1 (MQ=255)
                                                                                                                  cTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTGTGATCGGTTGCTGAACAg                               >  1:1532469/1‑150 (MQ=255)
                                                                                                                   tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                              <  1:4843/150‑1 (MQ=255)
                                                                                                                   tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                              >  1:526154/1‑150 (MQ=255)
                                                                                                                    cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCt                             >  2:417488/1‑150 (MQ=255)
                                                                                                                       aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCGCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1577968/150‑1 (MQ=255)
                                                                                                                       aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                          <  2:1065440/150‑1 (MQ=255)
                                                                                                                        ggccaaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTTAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCttttt                         >  1:328686/7‑150 (MQ=38)
                                                                                                                        ggccaaaTTGGGTGTCAGCGTTAATTCAGGCACCCAATTTGGCGTCTTAATGCAGGCAGCACTGCTGAACTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCGTTGATCGGTTGCTGAACAGCttttt                         >  1:292681/7‑150 (MQ=255)
                                                                                                                          ccTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGGACAGCTTTTTcc                       <  1:804615/150‑1 (MQ=255)
                                                                                                                           cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  1:1366878/1‑150 (MQ=255)
                                                                                                                           cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      >  2:1733832/1‑150 (MQ=255)
                                                                                                                           cTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCt                      <  1:342353/150‑1 (MQ=255)
                                                                                                                             tATTGGGTGCCAGAATTAACGCTGACACCCAAATTGTCCTCTTAATGCAGGCAGCACTGCTTAAAATTCTTAACACTACCGCACGGGCAAGGATCGTTACGCCAAGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGc                    >  2:739219/1‑150 (MQ=255)
                                                                                                                              tttGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAATGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   <  1:453864/149‑1 (MQ=255)
                                                                                                                              aTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   >  2:605615/1‑150 (MQ=255)
                                                                                                                              aTTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                   <  1:969636/150‑1 (MQ=255)
                                                                                                                                 gggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGAt                >  1:786574/1‑150 (MQ=255)
                                                                                                                                  ggTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               >  1:1449368/1‑150 (MQ=255)
                                                                                                                                  ggTGCCAGAATTAACGCTGACACCCATTGTGGCCTCTTAATGCAGGCCGCACTGCTTAAATTTCTTCCCACTACCGCCCGGGCAAGGATCGTTACGCCCCGGGTTCTCATCTGCTTAGATCGCTTGCTGCACCGCTGTTTCCTGCGAATg               >  1:175496/1‑150 (MQ=255)
                                                                                                                                  ggTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               >  2:1374435/1‑150 (MQ=255)
                                                                                                                                  ggTGCCAGAATTAACGCTGAAACCCAATTTGGCCTCTTAATGCAGTCAGCACTGCTTAAATTTCTTAACACTACCTCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg               <  2:1014670/150‑1 (MQ=255)
                                                                                                                                    tGCCAGAATTCACGCGGACACCCAATTTGGCCTCTTAAGGCAGGCAGCACAGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAGCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             <  1:77327/150‑1 (MQ=255)
                                                                                                                                    tGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg             >  1:1776714/1‑150 (MQ=255)
                                                                                                                                     gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  1:730373/1‑150 (MQ=255)
                                                                                                                                     gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCATAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTATGATCGGTTGCTGAACAGCTTTATCCTGCGGATgcgc            >  2:1406890/1‑150 (MQ=255)
                                                                                                                                     gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAATGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc            >  2:65715/1‑150 (MQ=255)
                                                                                                                                      ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGcc           >  1:1590674/1‑150 (MQ=255)
                                                                                                                                       cAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCa          >  1:841057/1‑150 (MQ=255)
                                                                                                                                              aaCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGATTAAATTTCTTACAACTACCGCACGGGCAAGGATCGTTACGCCCCGGATTATATTATGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCCGTa   >  2:142070/1‑150 (MQ=255)
                                                                                                                                               aCGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTAGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAc  >  1:379144/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTAC  >  NC_000913/1990788‑1991080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: