Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,999,231 T→A 8.7% T84S (ACT→TCT)  dcyD ← D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,2310TA 8.7% 294.1 / 13.5 111T84S (ACT→TCT) dcyDD‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (55/46);  new base A (8/2);  total (63/48)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 111 total aligned reads displayed.

GTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATT  >  NC_000913/1999083‑1999379
                                                                                                                                                    |                                                                                                                                                    
gTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTc                                                                                                                                                     <  1:1238439/150‑1 (MQ=255)
 tCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCt                                                                                                                                                    <  1:127795/150‑1 (MQ=255)
   aGTGCGTCGCACATTTCAATCTGTGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGc                                                                                                                                                  <  1:1656599/150‑1 (MQ=255)
   aGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGc                                                                                                                                                  <  2:217347/150‑1 (MQ=255)
    gTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCTATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCTCGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGcc                                                                                                                                                 <  1:903044/150‑1 (MQ=255)
      gCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGGTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGc                                                                                                                                               <  1:960902/150‑1 (MQ=255)
         gcGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcaca                                                                                                                                            <  1:319385/149‑1 (MQ=255)
          cGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtc                                                                                                                                           <  1:1606086/150‑4 (MQ=255)
           gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATg                                                                                                                                          >  1:710910/1‑150 (MQ=255)
            cacaTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACCTgg                                                                                                                                         >  1:1228936/1‑150 (MQ=255)
            cacaTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGAGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGATTGCCAGCGACTGCGGCAGTCTGCCGCACATgg                                                                                                                                         >  2:1678889/1‑150 (MQ=255)
              caTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGtt                                                                                                                                       >  2:1037547/1‑150 (MQ=255)
               aTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTa                                                                                                                                      >  1:564080/1‑150 (MQ=255)
                tttCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAg                                                                                                                                     >  2:1054294/1‑150 (MQ=255)
                  tCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGAc                                                                                                                                   >  2:758028/1‑150 (MQ=255)
                   cAATCTGGGTATTGCACAGATCCAGCAACAACCGATTGCCGTTGGTTAAATAGTCTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGAca                                                                                                                                  <  1:354334/150‑2 (MQ=255)
                     aTCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTgg                                                                                                                                <  2:1012518/150‑1 (MQ=255)
                       cTGGGTATTGAACAGATCCAGCAACAAGCGAATGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGAt                                                                                                                              <  1:407241/150‑1 (MQ=255)
                          ggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGc                                                                                                                           <  2:1411376/150‑1 (MQ=255)
                              ttGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCg                                                                                                                       >  2:1565853/1‑150 (MQ=255)
                               tGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCgg                                                                                                                      >  1:457725/1‑150 (MQ=255)
                               agAACAGATCCAGCAAGAAGCGAGTGCCGTTCGTGAAATAGTTTTCAGCGGATGTGCCAATACGATTTTCCAGCAGCGCCACGCAGTGAAGACCGAGTTAGTCAGCGACTGAGGCAGTCTGCCGCACATGGTTACAGTGTATCGcagctg                                                                                                                      >  2:225190/2‑145 (MQ=255)
                                gAACAGATCCTGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc                                                                                                                     >  2:920402/1‑150 (MQ=255)
                                gAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc                                                                                                                     >  2:1659337/1‑150 (MQ=255)
                                  aCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTTCAGACCGAGTTTGGCTGCGACTGAGGTAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAg                                                                                                                   <  1:952247/150‑1 (MQ=255)
                                     gATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCAGGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTTCCGCAAATGGTTAGACTGGTTCGCAGCGGctttaa                                                                                                                >  2:317247/1‑145 (MQ=255)
                                     gATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa                                                                                                                >  1:708893/1‑150 (MQ=255)
                                     gATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa                                                                                                                >  1:639678/1‑150 (MQ=255)
                                      aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAAt                                                                                                               <  1:1530008/150‑1 (MQ=255)
                                       tCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATc                                                                                                              >  1:1720972/1‑150 (MQ=255)
                                       accAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCACCCCGGCAGTAATc                                                                                                              <  2:470134/149‑1 (MQ=255)
                                        ccAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCa                                                                                                             >  1:1259846/1‑150 (MQ=255)
                                           gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCg                                                                                                          <  2:415813/150‑1 (MQ=255)
                                           gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCg                                                                                                          >  1:663459/1‑150 (MQ=255)
                                            caacaaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGt                                                                                                         <  2:27672/150‑1 (MQ=255)
                                            caacaaGCGACTGGCGTTGGTTTAATAGTGTTCTGCGGGTGAGACAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGt                                                                                                         <  2:308521/150‑1 (MQ=255)
                                             aacaaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCCGCGTa                                                                                                        >  1:365410/1‑150 (MQ=255)
                                               caaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATc                                                                                                      >  1:1552771/1‑150 (MQ=255)
                                                aaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGTGTATCg                                                                                                     >  1:1220370/1‑150 (MQ=255)
                                                 aGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCgg                                                                                                    <  2:1177260/150‑1 (MQ=255)
                                                   cGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGcc                                                                                                  >  1:1345056/1‑149 (MQ=255)
                                                     tttGCCGTTGGTTAAATAGTTTTCTGCGTTTGTGCCAATAGGATTTTCCAGCAGAGCCACGCAGTGCAGACCGACTTTGGCAGCGACTGAGGAAGTCTGCCGCACATGGTTAGAGTGGATCGCCCCGGCAGTAATCAGCGTATCGGCAcc                                                                                                <  2:462834/149‑1 (MQ=255)
                                                        gCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTc                                                                                             >  1:828042/1‑150 (MQ=255)
                                                         ccGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCa                                                                                            >  1:1372128/1‑150 (MQ=255)
                                                            ttGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGc                                                                                         <  1:758028/150‑1 (MQ=255)
                                                              ggTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGAACGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCag                                                                                       >  1:819088/1‑150 (MQ=255)
                                                                ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCagag                                                                                     <  1:621214/150‑1 (MQ=255)
                                                                  aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCa                                                                                   >  1:614898/1‑150 (MQ=255)
                                                                  aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGAGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGAGCGCCCCGGCAGTAATCAGCCTTTCGGCACCTTCACGCAGAGCa                                                                                   >  1:775500/1‑150 (MQ=255)
                                                                     tAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACAGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGCCTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCt                                                                                >  2:1139447/1‑150 (MQ=255)
                                                                       gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCCCGCAGAGCATCTGc                                                                              >  2:969403/1‑150 (MQ=255)
                                                                       gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGc                                                                              >  2:250673/1‑150 (MQ=255)
                                                                       gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGc                                                                              >  2:1252240/1‑150 (MQ=255)
                                                                       gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGACCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGc                                                                              >  2:871837/1‑150 (MQ=255)
                                                                        ttttCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGcc                                                                             <  2:710910/150‑1 (MQ=255)
                                                                           tCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCcgcg                                                                          <  1:452822/150‑1 (MQ=255)
                                                                            cTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGAATCTGCCGCga                                                                         >  2:501891/1‑150 (MQ=255)
                                                                                    gtgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTcc                                                                 <  2:26/150‑1 (MQ=255)
                                                                                    gtgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTcc                                                                 >  2:668289/1‑150 (MQ=255)
                                                                                        cAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCt                                                             >  1:1696930/1‑150 (MQ=255)
                                                                                           tAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTAc                                                          <  1:150562/150‑1 (MQ=255)
                                                                                             ggATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCCCCTTCACGCAGAGCATCTGCCGCGAGAAATTCCCGCTTACGt                                                        >  1:1121619/1‑150 (MQ=255)
                                                                                              gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTa                                                       >  1:1583006/1‑150 (MQ=255)
                                                                                                  ttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAAtgt                                                   >  2:770171/1‑148 (MQ=255)
                                                                                                    ccAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCTACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAt                                                 <  2:229160/150‑1 (MQ=255)
                                                                                                     cagcagCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt                                                <  2:1586086/150‑1 (MQ=255)
                                                                                                         agcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgccg                                            <  1:920402/150‑1 (MQ=255)
                                                                                                            gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCCCCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGccc                                         >  2:1224025/1‑150 (MQ=255)
                                                                                                            gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGccc                                         >  1:1419324/1‑150 (MQ=255)
                                                                                                              cACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCAt                                       >  1:821187/1‑150 (MQ=255)
                                                                                                                cGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTg                                     <  1:1054294/150‑1 (MQ=255)
                                                                                                                   aGTGCAGACCGAGTTTGGCAGCGCCTGCGGCAGTCTGCCGCACAGGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCCCGCAGAGCATCTGCCGCGAGAAATTCCCGCTTACGTAATTTATTGCCGCCCATTGCCa                                  >  1:931782/1‑150 (MQ=255)
                                                                                                                       cAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATggg                              <  2:1322687/150‑1 (MQ=255)
                                                                                                                        aGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCCGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg                             <  1:1130323/150‑1 (MQ=255)
                                                                                                                         gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGCAATTCCAGCGTACGTAATTTATTGCCGCCCATTGCCAtggtgg                            >  2:119945/1‑150 (MQ=255)
                                                                                                                         gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATggggg                            <  1:1780311/150‑1 (MQ=255)
                                                                                                                            cGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTgc                         >  2:162521/1‑149 (MQ=255)
                                                                                                                                 ttGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCa                    <  1:1425042/150‑1 (MQ=255)
                                                                                                                                  tGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCAt                   <  2:1720972/150‑1 (MQ=255)
                                                                                                                                   ggCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATc                  <  2:708893/150‑1 (MQ=255)
                                                                                                                                   ggCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATc                  >  1:1797106/1‑150 (MQ=255)
                                                                                                                                    gCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATcc                 >  2:468331/1‑150 (MQ=255)
                                                                                                                                    gCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTAGCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATcc                 <  1:1043510/150‑1 (MQ=255)
                                                                                                                                      accatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTTCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCAT‑GGGGTGACGTCATCCCGt              >  2:904310/7‑150 (MQ=255)
                                                                                                                                      accatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCCTCCCg               >  1:674403/7‑150 (MQ=255)
                                                                                                                                       ccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGt              >  2:499215/6‑150 (MQ=255)
                                                                                                                                        cGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCGCCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTTCGTAAATTATTGCCGACCCTTGCCATGGGGGTGACGTCATCCCGtt             >  1:178155/1‑150 (MQ=255)
                                                                                                                                        cGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt             >  1:1640328/1‑150 (MQ=255)
                                                                                                                                          tgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtg           >  1:1682202/3‑150 (MQ=255)
                                                                                                                                          aCTGCTGCAGTCTGCCTCACATGGTTAGACTGGATCGCCGCGGCAGTAATCAGCGTTTCGGCACGTTCACTCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTTTTTCCGCCCATTGCCATGGTGGTGACGTCATCCCGTTtg           <  2:564080/150‑1 (MQ=255)
                                                                                                                                          aCTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCGTCCCGTTtg           <  2:639678/150‑1 (MQ=255)
                                                                                                                                           gtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCCCCTTCCCGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGAAATTAATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGGTtga          >  1:358135/2‑150 (MQ=255)
                                                                                                                                           gtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTGACGTAATTTATTGCCGCCCCTTGCCATGGGGGTGACGTCATCCCGTTtgt          >  2:103971/2‑149 (MQ=255)
                                                                                                                                            tGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat         >  1:1406298/1‑150 (MQ=255)
                                                                                                                                               ggCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCGTCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaa      <  1:1659337/150‑1 (MQ=255)
                                                                                                                                               ggCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCCGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaa      >  2:1076316/1‑150 (MQ=255)
                                                                                                                                                gCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaaa     <  1:54211/150‑1 (MQ=255)
                                                                                                                                                  aGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCCCGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAt   <  2:127100/150‑1 (MQ=255)
                                                                                                                                                   gTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCCTCCCGTTTGATGAAAAtt  >  2:941095/1‑150 (MQ=255)
                                                                                                                                                   gTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAtt  >  2:560078/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATT  >  NC_000913/1999083‑1999379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑