Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,725 T→A 5.9% intergenic (+101/‑213) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,7250TA 5.9% 339.3 / 7.1 115intergenic (+101/‑213)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (55/53);  new base A (4/3);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 117 total aligned reads displayed.

TGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTA  >  NC_000913/2044581‑2044874
                                                                                                                                                |                                                                                                                                                     
tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAATATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                  <  2:540260/150‑1 (MQ=255)
  ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGt                                                                                                                                                >  1:834041/1‑150 (MQ=255)
   tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGATCATTGTTTTTTAACGGATAGCGGGTTTTTACCATCTTAAGCGCCCTCGCCCTTTATGGtt                                                                                                                                               >  2:1392011/1‑150 (MQ=255)
    aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTg                                                                                                                                              <  2:1249905/150‑1 (MQ=255)
    aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTg                                                                                                                                              >  2:804801/1‑150 (MQ=255)
      tCCGTATGTAACTGGTTCGAGTCCAGTAAGAGGAGCCAAATTCTAAAAAATCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGAGGGTTTTTAACATCTTAAGAGCCCTCGACCTTTATGGTTGAg                                                                                                                                            >  2:1421535/1‑150 (MQ=255)
       ccGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCATATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                           <  1:1257492/150‑1 (MQ=255)
       ccGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                           >  1:1251394/1‑150 (MQ=255)
        cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                          <  1:1403141/150‑1 (MQ=38)
        cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                          <  1:565658/150‑1 (MQ=38)
          tATGTGACTGGTTCGAGTCCAGTCAGAGGAGCCTATTTCTTAAAATTCGCTTTTTTAGCGCAATGTCAGTGACCTTAGTTGTACATTGTTTTTTAACGGTTAGCGGGTTTTTATCATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGCg                                                                                                                                        <  1:1214928/150‑1 (MQ=255)
               cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCTCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCTCCCTCGACCTTTATGGTTGAGGGCGttttt                                                                                                                                   >  1:1519547/1‑149 (MQ=255)
                aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGc                                                                                                                                  <  2:441764/150‑1 (MQ=255)
                   ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                               >  1:1598913/1‑150 (MQ=255)
                    gtgtgAGTCCAGTCAGCGGTCCCAAATTCTAAAAATTGGCTTTTTTATCGCATTGTAACTGACCTTAGTTGAACATTGTTTTTTAACGGATCGCGGGTTTTTACCTTGTTAACCGGCCTCGTCTTTTATGGTTGAGGGCGTTTTGCTATg                                                                                                                              <  2:1702639/146‑1 (MQ=255)
                    gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATg                                                                                                                              <  1:1575927/150‑1 (MQ=255)
                            ccAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                      >  1:408499/1‑150 (MQ=255)
                               gTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc                                                                                                                   >  1:1520564/1‑150 (MQ=255)
                                 cAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCa                                                                                                                 >  1:1545901/1‑150 (MQ=255)
                                     ggagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCTCCAtttt                                                                                                             >  1:1452205/1‑150 (MQ=255)
                                      gagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTc                                                                                                            <  2:185272/150‑1 (MQ=255)
                                       agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcc                                                                                                           <  1:1385985/150‑1 (MQ=255)
                                       agCCAAATTCTAAAAATTCGCTATTTTAGCTCAATTTCACTTACCTTAGTTGAAAATTGTTTAATAACGGATAGCGTGTTTTTAACATCTTAAGCGCCCTCGACATTTATCCTAGAGGCCGTTATGCTATTCACGCCATCACCATTTTcc                                                                                                           >  2:1327557/1‑150 (MQ=255)
                                        gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                          <  2:1744347/150‑1 (MQ=255)
                                        gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                          >  2:1119846/1‑150 (MQ=255)
                                        gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                          >  2:1176791/1‑150 (MQ=255)
                                          cAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCACCt                                                                                                        >  1:195326/1‑150 (MQ=255)
                                              ttCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTATTTGAACATTGTCTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGGTTTGCTATGAACGCCATCACCATTTTCCCCTCGAt                                                                                                    <  1:922050/150‑1 (MQ=255)
                                              ttCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGAt                                                                                                    <  2:1621990/150‑1 (MQ=255)
                                                cTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATta                                                                                                  >  2:663048/1‑150 (MQ=255)
                                                 tAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                 <  1:551206/150‑1 (MQ=255)
                                                       ttCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACt                                                                                           >  1:1059357/1‑150 (MQ=255)
                                                           cTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                       >  2:228163/1‑150 (MQ=255)
                                                           cTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                       <  2:870544/150‑1 (MQ=255)
                                                           cTTTTTTAGCGCAATGTCACTGACCGTAGTTGAACATTGTTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGACCGCCATCACCCTTTTCCCCTCGCTTATAAAACTTGAg                                                                                       <  2:1595824/150‑1 (MQ=255)
                                                              ttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTa                                                                                    >  2:234640/1‑150 (MQ=255)
                                                               tttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAt                                                                                   <  1:820156/150‑1 (MQ=255)
                                                                 tatcgcAATGTCACTGACCTAAGATGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                 <  2:866974/147‑1 (MQ=255)
                                                                 tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                 <  2:1457348/150‑1 (MQ=255)
                                                                  aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCa                                                                                >  1:754176/1‑150 (MQ=255)
                                                                  aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCa                                                                                >  1:1010171/1‑150 (MQ=255)
                                                                   gcgcAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGAAAGCGGCTTATTAACATCTTAAGCGCCATCGACCTTTATGGTTGAGGGCTTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCat                                                                               >  1:981900/1‑149 (MQ=255)
                                                                      cAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagtag                                                                            >  2:629642/1‑150 (MQ=255)
                                                                        aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTAGATTATAAAACTTGAGTTATTCAGTAGtc                                                                          >  1:1361930/1‑150 (MQ=255)
                                                                         tGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtct                                                                         <  1:1401831/150‑1 (MQ=255)
                                                                           tCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcc                                                                       >  2:1798221/1‑150 (MQ=255)
                                                                            cACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCACCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                                                      >  2:111929/1‑150 (MQ=255)
                                                                             aCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcccc                                                                     >  1:1115988/1‑150 (MQ=255)
                                                                                gaacTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGGTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctct                                                                  <  2:1405069/147‑1 (MQ=255)
                                                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctct                                                                  >  1:1193277/1‑150 (MQ=255)
                                                                                   cTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGc                                                               <  1:709675/150‑1 (MQ=255)
                                                                                   cTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGc                                                               >  1:646651/1‑150 (MQ=255)
                                                                                         tGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTGATTCAGTAGTCTCCCCTCTTGCAACTcc                                                         >  2:1704817/1‑149 (MQ=255)
                                                                                          gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcac                                                        >  2:39150/1‑150 (MQ=255)
                                                                                             cATTGTTTTTTAACTGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                     <  1:1481729/150‑1 (MQ=255)
                                                                                                   tttttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAAc                                               <  1:1538778/150‑1 (MQ=255)
                                                                                                    ttttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACt                                              <  2:1783217/150‑1 (MQ=255)
                                                                                                     tttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTg                                             >  1:970868/1‑150 (MQ=255)
                                                                                                      ttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                            >  2:767489/1‑150 (MQ=255)
                                                                                                      ttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGACATCACCTTTTTCACCTCGATTTTAAAAGTTGAGTTATTCAGTAGTCTCCCATCTTGCAACTCACACCGAAAACTGc                                            >  1:335938/1‑150 (MQ=255)
                                                                                                       tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGTGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGcc                                           <  1:1163821/150‑1 (MQ=255)
                                                                                                         aCGGATAGCGGGTTTTTAACATCTTAAGTGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTa                                         >  1:1797277/1‑150 (MQ=255)
                                                                                                                  gggTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAGTCAGTAGTCTCCCCTCTTGCAACTAACACCCCAAAGTGCCTATCGAATAGt                                >  1:249798/1‑150 (MQ=255)
                                                                                                                  gggTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGt                                <  2:140461/150‑1 (MQ=255)
                                                                                                                    ttttttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtta                              <  2:1623992/149‑1 (MQ=255)
                                                                                                                    gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtta                              <  2:1059357/150‑1 (MQ=255)
                                                                                                                     tttttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttat                             <  2:686416/150‑1 (MQ=255)
                                                                                                                       tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                           <  2:1251394/150‑1 (MQ=255)
                                                                                                                       tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                           <  1:663048/150‑1 (MQ=255)
                                                                                                                        ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttattta                          >  2:1801542/1‑148 (MQ=255)
                                                                                                                        ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTaa                          >  1:748540/1‑150 (MQ=255)
                                                                                                                          aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAAGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAtt                        >  2:1204875/1‑150 (MQ=255)
                                                                                                                             aTCTTAAGCTCCCTCGAACTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTTTAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTc                     <  1:1235271/150‑1 (MQ=255)
                                                                                                                             aTCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTc                     <  1:742074/150‑1 (MQ=255)
                                                                                                                              tCTTAATCGCCCTCGCCCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAACCTGCCTAACGAAAAGTTATTAATTTTCa                    >  1:1621882/1‑150 (MQ=255)
                                                                                                                              tCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGATATTCAGTAGTCTCCCCTCTTTCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCa                    <  2:1154218/150‑1 (MQ=255)
                                                                                                                              tCTTAAGCGCCCTCGACCTTTATGGTGGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCa                    <  1:546100/150‑1 (MQ=255)
                                                                                                                               cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCaa                   <  1:1119846/150‑1 (MQ=255)
                                                                                                                               cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAACATTTTCACCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCaa                   >  2:1181735/1‑150 (MQ=255)
                                                                                                                                ttAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAAt                  >  1:965375/1‑150 (MQ=255)
                                                                                                                                ttAAGCGCCCTCGAACTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTATCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAGCTAACACACAAAAATGCCTAACGAAAAGTTATAAATTTTCaaa                  >  1:1620786/1‑149 (MQ=255)
                                                                                                                                 tAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATc                 >  2:985829/1‑150 (MQ=255)
                                                                                                                                  aaGCGCCCTCGAACTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCACTGGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGAAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCa                <  2:704786/150‑1 (MQ=255)
                                                                                                                                   aGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCat               >  1:1264158/1‑150 (MQ=255)
                                                                                                                                    gcgcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCata              >  2:1745787/1‑150 (MQ=255)
                                                                                                                                     cgcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCatat             <  2:1127492/150‑1 (MQ=255)
                                                                                                                                     cgcCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCatat             >  2:1494130/1‑150 (MQ=255)
                                                                                                                                      gcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt            <  2:834041/150‑1 (MQ=255)
                                                                                                                                      gcCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCACCTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt            >  1:513482/1‑150 (MQ=255)
                                                                                                                                         cTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCt         >  2:941442/1‑150 (MQ=255)
                                                                                                                                          tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa        <  1:1280202/150‑1 (MQ=255)
                                                                                                                                          tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa        >  1:1597047/1‑150 (MQ=255)
                                                                                                                                          tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa        <  2:1086579/150‑1 (MQ=255)
                                                                                                                                          tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa        <  1:228163/150‑1 (MQ=255)
                                                                                                                                           cGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTTACGAAAAGTTATTAATTTTCAATCATATTGCTAt       >  1:55272/1‑150 (MQ=255)
                                                                                                                                              ccTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAg    <  2:1595884/150‑1 (MQ=255)
                                                                                                                                               cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt   <  2:408499/150‑1 (MQ=255)
                                                                                                                                               cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt   <  2:1115988/150‑1 (MQ=255)
                                                                                                                                                ttttTGGTTGATGGCGTTTTGCTATGTACTCCATCACCATTTTCCCATCGATTATAAAACTTGAGTTATTCAGTAGCCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTa  <  1:1327557/150‑1 (MQ=255)
                                                                                                                                                tttATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTa  <  1:1327561/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTA  >  NC_000913/2044581‑2044874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: