Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,733 T→C 6.0% intergenic (+109/‑205) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,7330TC 6.0% 345.7 / 9.4 118intergenic (+109/‑205)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (56/53);  new base C (4/3);  total (61/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

Only 100 of 119 total aligned reads displayed.

TAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACA  >  NC_000913/2044584‑2044880
                                                                                                                                                     |                                                                                                                                                   
tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGATCATTGTTTTTTAACGGATAGCGGGTTTTTACCATCTTAAGCGCCCTCGCCCTTTATGGtt                                                                                                                                                     >  2:1392011/1‑150 (MQ=255)
 aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTg                                                                                                                                                    <  2:1249905/150‑1 (MQ=255)
 aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTg                                                                                                                                                    >  2:804801/1‑150 (MQ=255)
   tCCGTATGTCACTGGTTCGAGTCGAGTCAGAGGAGCCATATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAGATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAg                                                                                                                                                  <  2:594089/150‑1 (MQ=255)
    ccGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCATATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                                 <  1:1257492/150‑1 (MQ=255)
    ccGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                                 >  1:1251394/1‑150 (MQ=255)
     cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                                <  1:565658/150‑1 (MQ=38)
     cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                                <  1:1403141/150‑1 (MQ=38)
       tATGTGACTGGTTCGAGTCCAGTCAGAGGAGCCTATTTCTTAAAATTCGCTTTTTTAGCGCAATGTCAGTGACCTTAGTTGTACATTGTTTTTTAACGGTTAGCGGGTTTTTATCATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGCg                                                                                                                                              <  1:1214928/150‑1 (MQ=255)
            cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCTCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCTCCCTCGACCTTTATGGTTGAGGGCGttttt                                                                                                                                         >  1:1519547/1‑149 (MQ=255)
            cACTGGTGCGAGTCCAGTCAGAGGAGCCAAATGCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGCCCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                                         <  2:1728842/150‑1 (MQ=255)
             aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGc                                                                                                                                        <  2:441764/150‑1 (MQ=255)
                ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                                     >  1:1598913/1‑150 (MQ=255)
                 gtgtgAGTCCAGTCAGCGGTCCCAAATTCTAAAAATTGGCTTTTTTATCGCATTGTAACTGACCTTAGTTGAACATTGTTTTTTAACGGATCGCGGGTTTTTACCTTGTTAACCGGCCTCGTCTTTTATGGTTGAGGGCGTTTTGCTATg                                                                                                                                    <  2:1702639/146‑1 (MQ=255)
                 gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATg                                                                                                                                    <  1:1575927/150‑1 (MQ=255)
                         ccAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                            >  1:408499/1‑150 (MQ=255)
                            gTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc                                                                                                                         >  1:1520564/1‑150 (MQ=255)
                              cAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCa                                                                                                                       >  1:1545901/1‑150 (MQ=255)
                                  ggagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCTCCAtttt                                                                                                                   >  1:1452205/1‑150 (MQ=255)
                                   gagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTc                                                                                                                  <  2:185272/150‑1 (MQ=255)
                                    agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcc                                                                                                                 <  1:1385985/150‑1 (MQ=255)
                                    agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcc                                                                                                                 >  2:1327561/1‑150 (MQ=255)
                                    agCCAAATTCTAAAAATTCGCTATTTTAGCTCAATTTCACTTACCTTAGTTGAAAATTGTTTAATAACGGATAGCGTGTTTTTAACATCTTAAGCGCCCTCGACATTTATCCTAGAGGCCGTTATGCTATTCACGCCATCACCATTTTcc                                                                                                                 >  2:1327557/1‑150 (MQ=255)
                                     gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                                <  2:1744347/150‑1 (MQ=255)
                                     gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                                >  2:1119846/1‑150 (MQ=255)
                                     gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                                >  2:1176791/1‑150 (MQ=255)
                                       cAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCACCt                                                                                                              >  1:195326/1‑150 (MQ=255)
                                           ttCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTATTTGAACATTGTCTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGGTTTGCTATGAACGCCATCACCATTTTCCCCTCGAt                                                                                                          <  1:922050/150‑1 (MQ=255)
                                             cTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATta                                                                                                        >  2:663048/1‑150 (MQ=255)
                                              tAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                       <  1:551206/150‑1 (MQ=255)
                                                 aaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTTGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaa                                                                                                    <  1:1802490/150‑1 (MQ=255)
                                                    ttCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACt                                                                                                 >  1:1059357/1‑150 (MQ=255)
                                                        cTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                             <  2:870544/150‑1 (MQ=255)
                                                        cTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                             >  2:228163/1‑150 (MQ=255)
                                                        cTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                             <  2:1072763/150‑1 (MQ=255)
                                                        cTTTTTTAGCGCAATGTCACTGACCGTAGTTGAACATTGTTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGACCGCCATCACCCTTTTCCCCTCGCTTATAAAACTTGAg                                                                                             <  2:1595824/150‑1 (MQ=255)
                                                            tttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAt                                                                                         <  1:820156/150‑1 (MQ=255)
                                                              tatcgcAATGTCACTGACCTAAGATGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                       <  2:866974/147‑1 (MQ=255)
                                                              tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                       <  2:1457348/150‑1 (MQ=255)
                                                               aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCa                                                                                      >  1:1010171/1‑150 (MQ=255)
                                                               aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCa                                                                                      >  1:754176/1‑150 (MQ=255)
                                                                gcgcAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGAAAGCGGCTTATTAACATCTTAAGCGCCATCGACCTTTATGGTTGAGGGCTTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCat                                                                                     >  1:981900/1‑149 (MQ=255)
                                                                     aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTAGATTATAAAACTTGAGTTATTCAGTAGtc                                                                                >  1:1361930/1‑150 (MQ=255)
                                                                        tCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcc                                                                             >  2:1798221/1‑150 (MQ=255)
                                                                         cACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCACCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                                                            >  2:111929/1‑150 (MQ=255)
                                                                          aCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcccc                                                                           >  1:1115988/1‑150 (MQ=255)
                                                                             gaacTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGGTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctct                                                                        <  2:1405069/147‑1 (MQ=255)
                                                                             gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctct                                                                        >  1:1193277/1‑150 (MQ=255)
                                                                               ccTTAGTTGAACATTGTTATTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTg                                                                      >  2:1175039/1‑150 (MQ=255)
                                                                                cTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGc                                                                     <  1:709675/150‑1 (MQ=255)
                                                                                cTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGc                                                                     >  1:646651/1‑150 (MQ=255)
                                                                                      tGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTGATTCAGTAGTCTCCCCTCTTGCAACTcc                                                               >  2:1704817/1‑149 (MQ=255)
                                                                                       gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcac                                                              >  2:39150/1‑150 (MQ=255)
                                                                                          cATTGTTTTTTAACTGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                           <  1:1481729/150‑1 (MQ=255)
                                                                                              gTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAACaaaa                                                       >  2:224049/1‑150 (MQ=255)
                                                                                                tttttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAAc                                                     <  1:1545980/150‑1 (MQ=255)
                                                                                                 ttttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACt                                                    <  2:1783217/150‑1 (MQ=255)
                                                                                                  tttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTg                                                   >  1:970868/1‑150 (MQ=255)
                                                                                                   ttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                                  >  2:767489/1‑150 (MQ=255)
                                                                                                   ttAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGACATCACCTTTTTCACCTCGATTTTAAAAGTTGAGTTATTCAGTAGTCTCCCATCTTGCAACTCACACCGAAAACTGc                                                  >  1:335938/1‑150 (MQ=255)
                                                                                                    tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGTGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGcc                                                 <  1:1163821/150‑1 (MQ=255)
                                                                                                      aCGGATAGCGGGTTTTTAACATCTTAAGTGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTa                                               >  1:1797277/1‑150 (MQ=255)
                                                                                                               gggTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGt                                      <  2:140461/150‑1 (MQ=255)
                                                                                                                 ttttttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtta                                    <  2:1623992/149‑1 (MQ=255)
                                                                                                                  tttttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttat                                   <  2:686416/150‑1 (MQ=255)
                                                                                                                    tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                                 <  1:663048/150‑1 (MQ=255)
                                                                                                                     ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttattta                                >  2:1801542/1‑148 (MQ=255)
                                                                                                                     ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTaa                                >  1:748540/1‑150 (MQ=255)
                                                                                                                      tAACATCTTAAGCGCCCTCGCCCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAt                               >  2:1577435/1‑150 (MQ=255)
                                                                                                                       aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAAGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAtt                              >  2:1204875/1‑150 (MQ=255)
                                                                                                                        aCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGACCGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCACCTCACACCCAAAACTGCCTAACGAAAAGTTATTAAttt                             >  1:1298587/1‑150 (MQ=255)
                                                                                                                          aTCTTAAGCTCCCTCGAACTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTTTAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTc                           <  1:1235271/150‑1 (MQ=255)
                                                                                                                          aTCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTc                           <  1:742074/150‑1 (MQ=255)
                                                                                                                           tCTTAATCGCCCTCGCCCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAACCTGCCTAACGAAAAGTTATTAATTTTCa                          >  1:1621882/1‑150 (MQ=255)
                                                                                                                           tCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGATATTCAGTAGTCTCCCCTCTTTCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCa                          <  2:1154218/150‑1 (MQ=255)
                                                                                                                           tCTTAAGCGCCCTCGACCTTTATGGTGGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCa                          <  1:546100/150‑1 (MQ=255)
                                                                                                                            cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCaa                         <  1:1119846/150‑1 (MQ=255)
                                                                                                                            cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAACATTTTCACCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCaa                         >  2:1181735/1‑150 (MQ=255)
                                                                                                                             ttAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAAt                        >  1:965375/1‑150 (MQ=255)
                                                                                                                              tAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATc                       >  2:985829/1‑150 (MQ=255)
                                                                                                                               aaGCGCCCTCGAACTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCACTGGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGAAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCa                      <  2:704786/150‑1 (MQ=255)
                                                                                                                                aacgcCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACACAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCat                     >  1:1446859/3‑150 (MQ=255)
                                                                                                                                aGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCat                     >  1:1264158/1‑150 (MQ=255)
                                                                                                                                  cgcCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCatat                   >  2:1494130/1‑150 (MQ=255)
                                                                                                                                   gcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt                  <  2:834041/150‑1 (MQ=255)
                                                                                                                                   gcCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCACCTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt                  >  1:513482/1‑150 (MQ=255)
                                                                                                                                      cTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCt               >  2:941442/1‑150 (MQ=255)
                                                                                                                                       tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa              <  1:228163/150‑1 (MQ=255)
                                                                                                                                       tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa              >  1:1597047/1‑150 (MQ=255)
                                                                                                                                       tCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTa              <  2:1086579/150‑1 (MQ=255)
                                                                                                                                        cGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTTACGAAAAGTTATTAATTTTCAATCATATTGCTAt             >  1:55272/1‑150 (MQ=255)
                                                                                                                                           ccTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAg          <  2:1595884/150‑1 (MQ=255)
                                                                                                                                            cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt         <  2:408499/150‑1 (MQ=255)
                                                                                                                                            cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt         <  2:1115988/150‑1 (MQ=255)
                                                                                                                                             ttttTGGTTGATGGCGTTTTGCTATGTACTCCATCACCATTTTCCCATCGATTATAAAACTTGAGTTATTCAGTAGCCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTa        <  1:1327557/150‑1 (MQ=255)
                                                                                                                                             tttATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTa        <  1:1327561/150‑1 (MQ=255)
                                                                                                                                               tATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTAtt      >  1:943456/1‑150 (MQ=255)
                                                                                                                                                 tGGTTGAGGGCGTTTTGCTATGAACGCCATCCCCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTa    >  1:29164/1‑150 (MQ=255)
                                                                                                                                                  ggTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTTTTAATTTTCAATCATATTGCTATCAGTATTTAc   >  2:234990/1‑150 (MQ=255)
                                                                                                                                                   gTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTAAGTAGTCTCCCCTCTTGCAACTCACCCCCAAAACTGCATAACGAAAAGTAATTAAATTTCAATCATAATGCAATCACTATTTACa  >  1:351124/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACA  >  NC_000913/2044584‑2044880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: