Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 7.7% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT 7.7% 328.3 / 12.6 116I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (49/58);  new base T (5/4);  total (54/62)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 120 total aligned reads displayed.

CTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081539‑2081836
                                                                                                                                                     |                                                                                                                                                    
cTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATTTTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                      >  1:140838/1‑150 (MQ=255)
cTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTATTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                      >  1:317680/1‑150 (MQ=255)
 tttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTACCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGCTGCAAAAt                                                                                                                                                     >  1:53888/1‑150 (MQ=255)
  ttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt                                                                                                                                                    >  1:1491949/1‑150 (MQ=255)
    aCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                                                                  <  2:478639/150‑1 (MQ=255)
      cATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                                                                                >  2:1237396/1‑150 (MQ=255)
       aTCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCAAGAACTCTTGTTCCGTTCCAAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTACAGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                                                                               >  1:1141043/1‑150 (MQ=255)
        tCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTTTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAcc                                                                                                                                              >  1:765634/1‑150 (MQ=255)
         cAAGGGTATATTTGGCTATGATATGAGGAATTTCGGATTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTAGATATTATCTTTATCCCCATACCACATGCGTTCCGTTACGCCCTTTTTCCCACGTTGCAAAATTTTCACCg                                                                                                                                             >  1:1079308/1‑150 (MQ=255)
          aaGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgt                                                                                                                                            <  1:1470169/150‑1 (MQ=255)
           aGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTACAAAATTTGCACCgta                                                                                                                                           <  1:1343311/150‑1 (MQ=255)
           aGGGAATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGCTGCGTTCCGTTCCGACCTTTTTCCCACGGGGCAAATTTTGCACCgtg                                                                                                                                           <  1:1754290/150‑2 (MQ=255)
            gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                                                                          <  1:1115940/150‑3 (MQ=255)
             ggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtggg                                                                                                                                         <  2:969278/150‑4 (MQ=255)
             ggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCATGATCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTATTTCCCACGGTGCAAAATTTGCAACgtagt                                                                                                                                         >  1:1377291/1‑150 (MQ=255)
               tataTTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCTTCCAGAACTCTTGTTCAGTTAACATGTAGATATTTTCTTTATCTCAATACCAGATGCGTTCCGTGCCGCCCTTTTTCACACGGTGCAAAATTTGCACCGTAGTaa                                                                                                                                       >  1:111124/1‑150 (MQ=255)
                atatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCATTATCGCCATACCAGATGCGTTCAGTTACGACCTTTTTACCACGGTGCAAAATTTGCACCGTAGTaaa                                                                                                                                      >  2:1402991/1‑150 (MQ=255)
                 tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTATCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAg                                                                                                                                     <  2:589487/150‑1 (MQ=255)
                 tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAg                                                                                                                                     <  1:409183/150‑1 (MQ=255)
                 tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAg                                                                                                                                     <  1:1567355/150‑1 (MQ=255)
                       gCTTTGATATCTGGAATTTCGGCTTTCGGTAGCACCATCCAGATCTCTTGTTCCGTTCCCATGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGAACTTTTCCCTAGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                                                                              <  2:290894/150‑1 (MQ=255)
                        cTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                                                                              <  1:1748004/150‑1 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACGTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                                                                             <  1:242468/150‑1 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                                                                             <  2:1733349/150‑1 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                                                                             <  2:1740312/150‑1 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                                                                             <  1:522825/150‑1 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttggtg                                                                                                                             >  1:1163267/1‑150 (MQ=255)
                                tgtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCaa                                                                                                                      >  1:1166524/1‑150 (MQ=255)
                                 gtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAAc                                                                                                                     >  2:1321723/1‑150 (MQ=255)
                                 gtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAAc                                                                                                                     >  2:917684/1‑150 (MQ=255)
                                     aaTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTGGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAg                                                                                                                 <  2:1145804/150‑1 (MQ=255)
                                     aaTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAGCGCAg                                                                                                                 >  2:664982/1‑150 (MQ=255)
                                       tttCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTATATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCGGTa                                                                                                               <  1:397206/150‑1 (MQ=255)
                                           ggCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAttt                                                                                                           <  2:1508291/150‑1 (MQ=255)
                                              tttCGGTAGCACCATCCAGATCTCTTGTTCCGTTCCCAGGTAGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTGCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                                                                                                        <  2:1568107/150‑1 (MQ=255)
                                               ttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                       <  1:1640529/150‑1 (MQ=255)
                                                tCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                                                                      <  1:841755/150‑1 (MQ=255)
                                                tCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                                                                      <  1:572658/150‑1 (MQ=255)
                                                  ggTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                                                                    <  1:917684/150‑1 (MQ=255)
                                                     aGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                                                                                                 <  2:1643893/150‑1 (MQ=255)
                                                      gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATAGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGAAAAGATATTTTTAAACAAACGCAGTAATTATCTTGcatatt                                                                                                >  2:754223/1‑145 (MQ=255)
                                                            tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCAAACGCAGTAATTTTCTTGCCTCTTCCTCAc                                                                                          <  1:519094/150‑1 (MQ=255)
                                                             ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg                                                                                         >  1:1222637/1‑150 (MQ=255)
                                                             ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg                                                                                         <  1:328325/150‑1 (MQ=255)
                                                                   ctctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt                                                                                   <  2:687276/150‑1 (MQ=255)
                                                                   ctctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTATTGCCTCTTCCTCACGACCttt                                                                                   >  2:982325/1‑150 (MQ=255)
                                                                     ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg                                                                                 >  1:1241752/1‑150 (MQ=255)
                                                                     ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg                                                                                 <  2:754693/150‑1 (MQ=255)
                                                                       tGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                               <  2:1491949/150‑1 (MQ=255)
                                                                        gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTAATCCTCACTACCTTTTCCAc                                                                              >  2:288575/1‑150 (MQ=255)
                                                                         ttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGCCCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGACACCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACt                                                                             >  1:258086/1‑150 (MQ=255)
                                                                          tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTg                                                                            <  2:1282696/150‑1 (MQ=255)
                                                                          tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTg                                                                            <  1:934168/150‑1 (MQ=255)
                                                                            cgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTACGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCAGCTTCCTCACGACATTTTGCACTGTc                                                                          >  1:1083712/1‑150 (MQ=255)
                                                                            cgttccAAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGAAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTTATTTTCTTGCCTCTTCATCACTACCTTTTGCACTGTc                                                                          >  1:1097365/1‑150 (MQ=255)
                                                                              ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                                                                        <  1:815673/150‑1 (MQ=255)
                                                                              ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCATCTTCCTCACGACCTTTTGCACTGTCAg                                                                        >  2:833829/1‑150 (MQ=255)
                                                                                cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                      <  1:366902/150‑1 (MQ=255)
                                                                                 ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTCCCGCTTCATCACGACCTTTTGCACTGTCCGccc                                                                     >  1:724801/1‑150 (MQ=255)
                                                                                  cAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCACAACTCAGTAATATTCTTGCCTCTTCCTCACGACCTTTTGAACTGTCAGccac                                                                    >  2:96472/1‑148 (MQ=255)
                                                                                     gTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt                                                                 <  1:936502/150‑1 (MQ=255)
                                                                                       cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                               >  1:1417703/1‑150 (MQ=255)
                                                                                       cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                               <  2:715532/150‑1 (MQ=255)
                                                                                       cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                               >  2:575992/1‑150 (MQ=255)
                                                                                        gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc                                                              <  2:1373458/150‑1 (MQ=255)
                                                                                         atatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc                                                             <  2:566750/150‑1 (MQ=255)
                                                                                         atatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc                                                             <  1:526678/150‑1 (MQ=255)
                                                                                           atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCAc                                                           <  1:846364/150‑1 (MQ=255)
                                                                                                cTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAACTTTTGCACTGTCAGCCACCATTACCACTGCaa                                                      >  1:1444823/1‑150 (MQ=255)
                                                                                                   tATCGCCATACCAGATGCGTGCCGTACCGCCATTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAGTTTTGTTGCACCCAACGCAGTAATTTTCTTGCCTCTACCTCACGACCTTTTGCCCTGTCAGCCCCCATTACCACTGCAATgag                                                  >  1:978238/1‑150 (MQ=255)
                                                                                                      cGCCATACCAGCTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCACACGACCTTTTGCACTGTAAGCCCCCATTACCACTGCAAAGCgac                                                >  2:408576/1‑150 (MQ=255)
                                                                                                      cGCCATACCAGATGCGGTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgac                                                <  1:1464580/150‑1 (MQ=255)
                                                                                                       gCCATACCAGATGCGTTACGTTCCGAACTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCGCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCATTTGCACTCTCAGCCCCCATTACCACTCCAATGAgacg                                               >  1:545225/1‑150 (MQ=255)
                                                                                                         cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac                                             <  1:1670874/150‑1 (MQ=255)
                                                                                                           tACCATATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTATCTAGCCTCTTCCTCACGCCCTTTTGCACTGTCAGCCCCCATTACGCCTGCAATGAGAAGACGc                                           >  2:593938/1‑150 (MQ=255)
                                                                                                            aCCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCTTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTACTCACGACCTTTTGAACTTTCAGCCCCCATTACCACTTCAATTAGACGActct                                          >  1:264361/1‑150 (MQ=255)
                                                                                                              cAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTGc                                        >  2:1350869/1‑150 (MQ=255)
                                                                                                              cAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGc                                        >  2:1093702/1‑150 (MQ=255)
                                                                                                                   gCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATc                                   <  1:933853/150‑1 (MQ=255)
                                                                                                                          tttccgACCTTTTTCCCACGTTGCAAAATTTGCACCGTAGTAATGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGcc                            <  2:1141043/149‑1 (MQ=255)
                                                                                                                          gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGcc                            <  1:894663/150‑1 (MQ=255)
                                                                                                                           tgccgACCTTTTTCCCACGGGGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTTCAGCCg                           <  1:162946/148‑1 (MQ=255)
                                                                                                                              cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAg                        >  1:773547/1‑150 (MQ=255)
                                                                                                                               gACCTTTTTCCCACGGTGCAAAATTTGCACAGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGc                       <  2:117518/150‑1 (MQ=255)
                                                                                                                                 ccTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTGCCCATCTACAGCCGAAGCaa                     >  1:726838/1‑150 (MQ=255)
                                                                                                                                 ccTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGAAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCACATTACCACTGCAATGAGACTACGCTGCACATCTAAATCCGAAGCaa                     >  2:1016281/1‑150 (MQ=255)
                                                                                                                                    ttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                  <  1:1679571/150‑1 (MQ=255)
                                                                                                                                    ctttactaCGGTGCAAATTTTGCACCGTGGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                  >  2:418230/8‑150 (MQ=255)
                                                                                                                                     tttactaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgag                 >  2:1509316/7‑150 (MQ=255)
                                                                                                                                      ttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgaga                <  1:991960/150‑1 (MQ=255)
                                                                                                                                        cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCACCGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGCTCCGACGCGGCCCCTCGACAGCCGAAGCAATGAGAtt              >  2:1668432/1‑150 (MQ=255)
                                                                                                                                        actaCGGTGCAAATTTTGCACCGTGGGAAAGTTTTGTTGCCACCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCAACAGCCGAAGCAATGAGAtt              >  2:172834/4‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTGGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  1:844274/3‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGCCCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  2:1574327/3‑150 (MQ=255)
                                                                                                                                         ccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAACACTGAAATGAGACGACTCTGACAATCTACAGCCGAAGCAATGAGATTa             >  1:1482990/1‑150 (MQ=255)
                                                                                                                                            cGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAAc          <  1:1321723/150‑1 (MQ=255)
                                                                                                                                               tGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCg       >  1:855373/1‑150 (MQ=255)
                                                                                                                                                gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAGTACCACTCCAAGGCGACGCCGCTGCCCATCTACGGCCGAAGCCATGAGATTTCACCCgg      >  2:1091596/1‑150 (MQ=255)
                                                                                                                                                    aaTTTGCACGGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAcc  <  2:140838/150‑1 (MQ=255)
                                                                                                                                                    aaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCGATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAcc  <  2:317680/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081539‑2081836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: