Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,664 A→G 6.0% intergenic (‑139/+40) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6640AG 6.0% 351.1 / 9.0 117intergenic (‑139/+40)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (57/53);  new base G (5/2);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 121 total aligned reads displayed.

ATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085515‑2085812
                                                                                                                                                     |                                                                                                                                                    
aTTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCa                                                                                                                                                      >  1:1339358/1‑150 (MQ=255)
aTTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCa                                                                                                                                                      >  1:607473/1‑150 (MQ=255)
    aaaaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATg                                                                                                                                                  <  1:1550618/150‑1 (MQ=255)
     aaaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGt                                                                                                                                                 >  1:408542/1‑150 (MQ=255)
      aaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGtt                                                                                                                                                <  2:1228167/150‑1 (MQ=255)
        cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGttaa                                                                                                                                              >  1:1056315/1‑148 (MQ=255)
        cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCa                                                                                                                                              >  2:1285180/1‑150 (MQ=255)
          tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  1:720919/150‑1 (MQ=255)
          tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  2:1589507/150‑1 (MQ=255)
           aaaTTAATTAACCTGATGAATTTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           <  1:1531287/150‑1 (MQ=255)
           aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGGGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           <  2:1576814/150‑1 (MQ=255)
            aattaattAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTcc                                                                                                                                          >  2:1389001/1‑149 (MQ=255)
             attaattaACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGa                                                                                                                                         >  2:321487/1‑150 (MQ=255)
                 attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                     <  1:321487/150‑1 (MQ=255)
                   taaCCAGATGAATGTTAATGAGGATATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTTAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCgg                                                                                                                                   >  2:1731110/1‑150 (MQ=255)
                    aaacAGATGAATGTTAATGAGGAAATTATTAATGACTGGTGGAATGCAGACCAATAACAAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGATCGATACAAAAGAATAAAAAAACAGGAGCCAAATCTTCATTCGACTGCGGt                                                                                                                                  >  1:1194018/4‑150 (MQ=255)
                     aCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGtt                                                                                                                                 <  2:19584/150‑1 (MQ=255)
                         taagAATTTAAATTAGGAAATTATTCATGACTTGTGGAATGCAGACCAATAACCAAATTATCTAAATAAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                             <  1:228661/147‑1 (MQ=255)
                         taTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttt                                                                                                                             <  2:1796277/149‑1 (MQ=38)
                          aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCGGTttattt                                                                                                                            >  2:1778459/1‑149 (MQ=255)
                           tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGCCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                           >  1:902896/1‑150 (MQ=255)
                            gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  1:948113/150‑1 (MQ=255)
                            gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:739621/150‑1 (MQ=255)
                            gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:1629736/150‑1 (MQ=255)
                             aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAACTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:1241560/150‑1 (MQ=255)
                             aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGATTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  2:1532916/150‑1 (MQ=255)
                             aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  2:724604/150‑1 (MQ=255)
                             aaTGTTAATGAGGAAATTATTCATGAATGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGATGAGTTCGATACAAAAGAATAAAAACACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:106785/150‑1 (MQ=255)
                              aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                        <  1:1513538/150‑1 (MQ=255)
                               tGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGa                                                                                                                       >  2:1422741/1‑150 (MQ=255)
                                  tAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACTAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaaa                                                                                                                    >  2:1501090/1‑150 (MQ=255)
                                  tAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaaa                                                                                                                    <  2:1163136/150‑1 (MQ=255)
                                    aTGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAga                                                                                                                  <  2:400549/150‑1 (MQ=255)
                                     tGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgat                                                                                                                 >  2:814685/1‑150 (MQ=255)
                                        ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              <  2:1619625/150‑1 (MQ=255)
                                        ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              >  1:1390558/1‑150 (MQ=255)
                                           aataatTCATGAATGGTTGAATGATTACCAATAACAAAATTCTATAAATTAGAACATATGGTTATTAATGATGAGTTCGATACAAAATAATAATAAAACCGTAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTAt                                                                                                           <  1:192493/150‑1 (MQ=255)
                                              tattCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCt                                                                                                        <  2:1256361/150‑1 (MQ=255)
                                                 tCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACg                                                                                                     <  1:1197568/150‑1 (MQ=255)
                                                  cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGGCCCAATGGTTCATTCGACTCCGGTTTTTTATTATAAAAGATGTTTATggaactg                                                                                                    >  2:795570/1‑144 (MQ=255)
                                                  cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:372376/1‑150 (MQ=255)
                                                  cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:1794667/1‑150 (MQ=255)
                                                   aTGACTGGTGGAATGCAGACCAATAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTc                                                                                                   <  2:789868/150‑1 (MQ=255)
                                                       cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                               >  2:713543/1‑150 (MQ=255)
                                                       cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAAAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAAGTCTTCg                                                                                               >  1:168687/1‑150 (MQ=255)
                                                            ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                          <  1:1600167/150‑1 (MQ=255)
                                                             gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                         >  2:607310/1‑150 (MQ=255)
                                                                   aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAcc                                                                                   <  1:52486/150‑1 (MQ=255)
                                                                           aaCCAAATTCTCTAAATTATAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCAGGTTTATTATTAGAAAAGATGATTATGCTACTTCTTAGTACTGAGGAACCGGATTACg                                                                           >  2:50956/1‑150 (MQ=255)
                                                                           aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           >  2:1444059/1‑150 (MQ=255)
                                                                           aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           <  2:444445/150‑1 (MQ=255)
                                                                           aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           <  2:885774/150‑1 (MQ=255)
                                                                           aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           <  1:414786/150‑1 (MQ=255)
                                                                             ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                         >  1:788905/1‑150 (MQ=255)
                                                                              cAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCCATACAAAAGAATAAAAAAACCGGAGCCAAATGTACATTCGACTCCGGTTAATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGCATTACCGaa                                                                        >  1:1403353/1‑150 (MQ=255)
                                                                              aaaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGaa                                                                        <  1:143755/149‑1 (MQ=255)
                                                                                      ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:393949/1‑150 (MQ=255)
                                                                                      ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  2:1557299/150‑1 (MQ=255)
                                                                                      ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  2:1527886/1‑150 (MQ=255)
                                                                                      ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:578972/1‑150 (MQ=255)
                                                                                      ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               <  1:991978/150‑1 (MQ=255)
                                                                                      atAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  1:1461256/149‑1 (MQ=255)
                                                                                       tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               >  1:1601339/1‑150 (MQ=255)
                                                                                       tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTAATTCGAATCCGGTTTATTATTATAAAATATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACTGAAGCTCTTGGt                                                               >  1:248533/1‑150 (MQ=255)
                                                                                        aaaTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAATAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTg                                                              <  2:1257096/150‑1 (MQ=255)
                                                                                          aTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGAc                                                            >  1:377827/1‑150 (MQ=255)
                                                                                              gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACTCaa                                                        >  1:1663394/1‑150 (MQ=255)
                                                                                               aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGTTGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAg                                                       >  2:342802/1‑150 (MQ=255)
                                                                                                        ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                              >  1:1365650/1‑150 (MQ=255)
                                                                                                          attaATGAGGAGTTCGTTACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATGATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                            <  1:195236/150‑1 (MQ=255)
                                                                                                          attaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                            <  2:1040892/150‑1 (MQ=255)
                                                                                                           ataATGAGGTGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                           <  1:336493/149‑1 (MQ=255)
                                                                                                            taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCTGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCat                                          >  1:704330/1‑149 (MQ=255)
                                                                                                              aTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAc                                        <  1:1285180/150‑1 (MQ=255)
                                                                                                               tGAGGAGTTCGATACAAAATAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  2:697210/150‑1 (MQ=255)
                                                                                                               agaggatTTCGAAACAAAAGAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  1:1605594/149‑1 (MQ=255)
                                                                                                                         gATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGcc                             <  2:381420/150‑1 (MQ=255)
                                                                                                                          atccAAAAGAATAAACAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCTTACTGCGGAACCGGATTACGTAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGccc                            <  1:912629/147‑1 (MQ=255)
                                                                                                                          aTACAAAAGAATTAAAAAACAGGAGCCAAATGTTCATTCGAATCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGccc                            <  1:84195/150‑1 (MQ=255)
                                                                                                                            aCAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTTGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAg                          >  2:1226182/1‑150 (MQ=255)
                                                                                                                             cAAAAGAATAAAAAAACCGGAGCCAAATGTTCATGCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCGTCGTCCTGCGGACCCGGAGTCCGGAGGCTCTTGGTGACGCAAGCCAGGTAACTCAGACGGATAGCAGCCCAGa                         >  1:362039/1‑150 (MQ=255)
                                                                                                                              aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                        >  2:529878/1‑150 (MQ=255)
                                                                                                                               aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGATCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       >  1:1704677/1‑150 (MQ=255)
                                                                                                                               aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTATATCAGACCGATAGCAGCCCAGATc                       >  2:1540475/1‑150 (MQ=255)
                                                                                                                               aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       <  1:284282/150‑1 (MQ=255)
                                                                                                                               aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       <  1:652929/150‑1 (MQ=255)
                                                                                                                               aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATc                       >  1:1705660/1‑150 (MQ=255)
                                                                                                                                 aGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAg                     >  2:1304093/1‑150 (MQ=255)
                                                                                                                                   taataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCACGTAAATCAGACCGATAGCAGCCCAGATCAGAc                   >  2:1622528/8‑150 (MQ=38)
                                                                                                                                    aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                  >  2:1075424/1‑150 (MQ=255)
                                                                                                                                     ataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                 >  1:7568/6‑150 (MQ=38)
                                                                                                                                      aaaaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCa                >  1:563456/1‑150 (MQ=255)
                                                                                                                                        ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGa              >  1:1608786/3‑150 (MQ=38)
                                                                                                                                        aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa              <  2:666939/150‑1 (MQ=255)
                                                                                                                                             ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAt         >  1:25503/1‑150 (MQ=255)
                                                                                                                                              cGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGAACCAtt        >  2:377076/1‑150 (MQ=255)
                                                                                                                                               ggAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTg       >  1:1723525/1‑150 (MQ=255)
                                                                                                                                                  gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc    >  1:661341/1‑150 (MQ=255)
                                                                                                                                                    cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATAAGCCCCAGAACCATTGAGCtt  >  2:560294/1‑150 (MQ=255)
                                                                                                                                                    cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCtt  >  1:564586/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085515‑2085812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: