Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,669 T→A 5.6% intergenic (‑144/+35) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6690TA 5.6% 345.1 / 5.2 113intergenic (‑144/+35)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base T (55/51);  new base A (5/2);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

AAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085520‑2085812
                                                                                                                                                     |                                                                                                                                               
aaaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGt                                                                                                                                                 >  1:408542/1‑150 (MQ=255)
 aaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGtt                                                                                                                                                <  2:1228167/150‑1 (MQ=255)
   cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGttaa                                                                                                                                              >  1:1056315/1‑148 (MQ=255)
   cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCa                                                                                                                                              >  2:1285180/1‑150 (MQ=255)
     tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  1:720919/150‑1 (MQ=255)
     tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  2:1589507/150‑1 (MQ=255)
      aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           >  2:414786/1‑150 (MQ=255)
      aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGGGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           <  2:1576814/150‑1 (MQ=255)
       aattaattAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTcc                                                                                                                                          >  2:1389001/1‑149 (MQ=255)
            attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                     <  1:321487/150‑1 (MQ=255)
              taaCCAGATGAATGTTAATGAGGATATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTTAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCgg                                                                                                                                   >  2:1731110/1‑150 (MQ=255)
               aaacAGATGAATGTTAATGAGGAAATTATTAATGACTGGTGGAATGCAGACCAATAACAAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGATCGATACAAAAGAATAAAAAAACAGGAGCCAAATCTTCATTCGACTGCGGt                                                                                                                                  >  1:1194018/4‑150 (MQ=255)
                aCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGtt                                                                                                                                 <  2:19584/150‑1 (MQ=255)
                   aGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttat                                                                                                                              <  1:1735576/150‑1 (MQ=255)
                    taagAATTTAAATTAGGAAATTATTCATGACTTGTGGAATGCAGACCAATAACCAAATTATCTAAATAAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                             <  1:228661/147‑1 (MQ=255)
                    taTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttt                                                                                                                             <  2:1796277/149‑1 (MQ=38)
                    gattcaTGTTAATGAGGAAATTATTAATGACTGGTGGAATGCAGATAAATAACGAAATTCTCTAAATTATAACAAATGGTTATTAATGATGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                             <  2:780996/145‑1 (MQ=255)
                     aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                            >  1:595162/1‑150 (MQ=255)
                     aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCGGTttattt                                                                                                                            >  2:1778459/1‑149 (MQ=255)
                      tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGCCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                           >  1:902896/1‑150 (MQ=255)
                       gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:1629736/150‑1 (MQ=255)
                       gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  1:948113/150‑1 (MQ=255)
                       gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:739621/150‑1 (MQ=255)
                        aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAACTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:1241560/150‑1 (MQ=255)
                        aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttttatt                                                                                                                         <  2:1723286/150‑5 (MQ=38)
                        aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  2:724604/150‑1 (MQ=255)
                        aaTGTTAATGAGGAAATTATTCATGAATGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGATGAGTTCGATACAAAAGAATAAAAACACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:106785/150‑1 (MQ=255)
                         aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                        <  1:1513538/150‑1 (MQ=255)
                          tGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGa                                                                                                                       >  2:1422741/1‑150 (MQ=255)
                             tAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACTAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaaa                                                                                                                    >  2:1501090/1‑150 (MQ=255)
                             tAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaaa                                                                                                                    <  2:1163136/150‑1 (MQ=255)
                               aTGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAga                                                                                                                  <  2:400549/150‑1 (MQ=255)
                                tGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgat                                                                                                                 >  2:814685/1‑150 (MQ=255)
                                   ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              >  1:1390558/1‑150 (MQ=255)
                                   ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              >  1:1277386/1‑150 (MQ=255)
                                   ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              <  2:1619625/150‑1 (MQ=255)
                                         tattCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCt                                                                                                        <  2:1256361/150‑1 (MQ=255)
                                            tCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACg                                                                                                     <  1:1197568/150‑1 (MQ=255)
                                             cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGGCCCAATGGTTCATTCGACTCCGGTTTTTTATTATAAAAGATGTTTATggaactg                                                                                                    >  2:795570/1‑144 (MQ=255)
                                             cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:1794667/1‑150 (MQ=255)
                                             cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:372376/1‑150 (MQ=255)
                                              aTGACTGGTGGAATGCAGACCAATAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTc                                                                                                   <  2:789868/150‑1 (MQ=255)
                                                  cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAAAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAAGTCTTCg                                                                                               >  1:168687/1‑150 (MQ=255)
                                                    ggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAAGGAGGAGTTCGATTCAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAATATGATATTGCTACGTATTCGTa                                                                                             >  1:362701/1‑150 (MQ=255)
                                                       ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                          <  1:1600167/150‑1 (MQ=255)
                                                        gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                         >  2:607310/1‑150 (MQ=255)
                                                            gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                     >  2:1493414/1‑150 (MQ=255)
                                                              aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAcc                                                                                   <  1:52486/150‑1 (MQ=255)
                                                                      aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           <  2:444445/150‑1 (MQ=255)
                                                                      aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           >  2:1444059/1‑150 (MQ=255)
                                                                        ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                         >  1:788905/1‑150 (MQ=255)
                                                                         cAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCCATACAAAAGAATAAAAAAACCGGAGCCAAATGTACATTCGACTCCGGTTAATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGCATTACCGaa                                                                        >  1:1403353/1‑150 (MQ=255)
                                                                         aaaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGaa                                                                        <  1:143755/149‑1 (MQ=255)
                                                                                 ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  2:1557299/150‑1 (MQ=255)
                                                                                 ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  2:1527886/1‑150 (MQ=255)
                                                                                 ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:578972/1‑150 (MQ=255)
                                                                                 ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:393949/1‑150 (MQ=255)
                                                                                 ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               <  1:991978/150‑1 (MQ=255)
                                                                                 atAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  1:1461256/149‑1 (MQ=255)
                                                                                  tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               >  1:1601339/1‑150 (MQ=255)
                                                                                  tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTAATTCGAATCCGGTTTATTATTATAAAATATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACTGAAGCTCTTGGt                                                               >  1:248533/1‑150 (MQ=255)
                                                                                   aaaTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAATAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTg                                                              <  2:1257096/150‑1 (MQ=255)
                                                                                   aaaTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTg                                                              >  2:1560855/1‑150 (MQ=255)
                                                                                     aTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGAc                                                            >  1:377827/1‑150 (MQ=255)
                                                                                         gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACTCaa                                                        >  1:1663394/1‑150 (MQ=255)
                                                                                          aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGTTGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAg                                                       >  2:342802/1‑150 (MQ=255)
                                                                                           aCAAATGGTTTTTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGc                                                      >  1:253832/1‑150 (MQ=255)
                                                                                              aaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAg                                                   <  1:1419624/150‑1 (MQ=255)
                                                                                                     attaATGAGGAGTTCGTTACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATGATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                            <  1:195236/150‑1 (MQ=255)
                                                                                                     attaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                            <  2:1040892/150‑1 (MQ=255)
                                                                                                       taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCTGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCat                                          >  1:704330/1‑149 (MQ=255)
                                                                                                         aTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAc                                        <  1:417172/150‑1 (MQ=255)
                                                                                                         aTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAc                                        <  1:1285180/150‑1 (MQ=255)
                                                                                                          tGAGGAGTTCGATACAAAATAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  2:697210/150‑1 (MQ=255)
                                                                                                          tGAGGAGTTCGAAACAAAAGTATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCGCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  1:1598497/150‑1 (MQ=255)
                                                                                                          agaggatTTCGAAACAAAAGAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  1:1605594/149‑1 (MQ=255)
                                                                                                           gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCg                                      <  1:745126/150‑1 (MQ=255)
                                                                                                                    gATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGcc                             <  2:381420/150‑1 (MQ=255)
                                                                                                                     atccAAAAGAATAAACAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCTTACTGCGGAACCGGATTACGTAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGccc                            <  1:912629/147‑1 (MQ=255)
                                                                                                                       aCAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTTGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAg                          >  2:1226182/1‑150 (MQ=255)
                                                                                                                        cAAAAGAATAAAAAAACCGGAGCCAAATGTTCATGCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCGTCGTCCTGCGGACCCGGAGTCCGGAGGCTCTTGGTGACGCAAGCCAGGTAACTCAGACGGATAGCAGCCCAGa                         >  1:362039/1‑150 (MQ=255)
                                                                                                                         aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                        >  2:529878/1‑150 (MQ=255)
                                                                                                                          aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGATCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       >  1:1704677/1‑150 (MQ=255)
                                                                                                                          aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTATATCAGACCGATAGCAGCCCAGATc                       >  2:1540475/1‑150 (MQ=255)
                                                                                                                          aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       <  1:284282/150‑1 (MQ=255)
                                                                                                                          aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATc                       >  1:1705660/1‑150 (MQ=255)
                                                                                                                               aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                  >  2:1075424/1‑150 (MQ=255)
                                                                                                                               aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                  >  2:611352/1‑150 (MQ=255)
                                                                                                                                ataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                 >  1:7568/6‑150 (MQ=38)
                                                                                                                                 aaaaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCa                >  1:563456/1‑150 (MQ=255)
                                                                                                                                   ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGa              >  1:1608786/3‑150 (MQ=38)
                                                                                                                                   aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTTCGGAACCGGATTACGGAAGCTCATGGTGACTCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa              >  2:203803/1‑150 (MQ=255)
                                                                                                                                   aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa              <  2:666939/150‑1 (MQ=255)
                                                                                                                                        ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAt         >  1:25503/1‑150 (MQ=255)
                                                                                                                                         cGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGAACCAtt        >  2:377076/1‑150 (MQ=255)
                                                                                                                                         cGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAtt        <  2:1277386/150‑1 (MQ=255)
                                                                                                                                          ggAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTg       >  1:1723525/1‑150 (MQ=255)
                                                                                                                                             gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc    >  1:661341/1‑150 (MQ=255)
                                                                                                                                               cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATAAGCCCCAGAACCATTGAGCtt  >  2:560294/1‑150 (MQ=255)
                                                                                                                                               cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCtt  >  1:564586/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
AAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085520‑2085812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: