Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,670 T→A 6.6% intergenic (‑145/+34) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6700TA 6.6% 323.2 / 6.5 113intergenic (‑145/+34)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base T (54/51);  new base A (6/2);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085521‑2085812
                                                                                                                                                     |                                                                                                                                              
aaCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGtt                                                                                                                                                <  2:1228167/150‑1 (MQ=255)
  cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGttaa                                                                                                                                              >  1:1056315/1‑148 (MQ=255)
  cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCa                                                                                                                                              >  2:1285180/1‑150 (MQ=255)
    tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  1:720919/150‑1 (MQ=255)
    tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                            <  2:1589507/150‑1 (MQ=255)
     aaaTTAATTAACCTGATGAATTTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           <  1:1531287/150‑1 (MQ=255)
     aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           >  2:414786/1‑150 (MQ=255)
     aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGGGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                           <  2:1576814/150‑1 (MQ=255)
      aattaattAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTcc                                                                                                                                          >  2:1389001/1‑149 (MQ=255)
       attaattaACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGa                                                                                                                                         >  2:321487/1‑150 (MQ=255)
           attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                     <  1:321487/150‑1 (MQ=255)
             taaCCAGATGAATGTTAATGAGGATATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTTAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCgg                                                                                                                                   >  2:1731110/1‑150 (MQ=255)
              aaacAGATGAATGTTAATGAGGAAATTATTAATGACTGGTGGAATGCAGACCAATAACAAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGATCGATACAAAAGAATAAAAAAACAGGAGCCAAATCTTCATTCGACTGCGGt                                                                                                                                  >  1:1194018/4‑150 (MQ=255)
                  aGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttat                                                                                                                              <  1:1735576/150‑1 (MQ=255)
                   taTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttt                                                                                                                             <  2:1796277/149‑1 (MQ=38)
                   gattcaTGTTAATGAGGAAATTATTAATGACTGGTGGAATGCAGATAAATAACGAAATTCTCTAAATTATAACAAATGGTTATTAATGATGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                             <  2:780996/145‑1 (MQ=255)
                   gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                             >  2:1197568/1‑150 (MQ=255)
                    aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                            >  1:595162/1‑150 (MQ=255)
                    aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTCATTCGACTCCGGTttattt                                                                                                                            >  2:1778459/1‑149 (MQ=255)
                     tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGCCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                           >  1:902896/1‑150 (MQ=255)
                      gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:1629736/150‑1 (MQ=255)
                      gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  2:739621/150‑1 (MQ=255)
                      gAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                          <  1:948113/150‑1 (MQ=255)
                       aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAACTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:1241560/150‑1 (MQ=255)
                       aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGATTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  2:1532916/150‑1 (MQ=255)
                       aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttttatt                                                                                                                         <  2:1723286/150‑5 (MQ=38)
                       aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  2:724604/150‑1 (MQ=255)
                       aaTGTTAATGAGGAAATTATTCATGAATGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGATGAGTTCGATACAAAAGAATAAAAACACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                         <  1:106785/150‑1 (MQ=255)
                        aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                        <  1:1513538/150‑1 (MQ=255)
                         tGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGa                                                                                                                       >  2:1422741/1‑150 (MQ=255)
                            tAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaaa                                                                                                                    <  2:1163136/150‑1 (MQ=255)
                              aTGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAga                                                                                                                  <  2:400549/150‑1 (MQ=255)
                               tGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgat                                                                                                                 >  2:814685/1‑150 (MQ=255)
                                  ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              >  1:1277386/1‑150 (MQ=255)
                                  ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                              >  1:1390558/1‑150 (MQ=255)
                                     aataatTCATGAATGGTTGAATGATTACCAATAACAAAATTCTATAAATTAGAACATATGGTTATTAATGATGAGTTCGATACAAAATAATAATAAAACCGTAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTAt                                                                                                           <  1:192493/150‑1 (MQ=255)
                                        tattCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCt                                                                                                        <  2:1256361/150‑1 (MQ=255)
                                           tCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACg                                                                                                     <  1:1197568/150‑1 (MQ=255)
                                            cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGGCCCAATGGTTCATTCGACTCCGGTTTTTTATTATAAAAGATGTTTATggaactg                                                                                                    >  2:795570/1‑144 (MQ=255)
                                            cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:372376/1‑150 (MQ=255)
                                            cATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGt                                                                                                    >  1:1794667/1‑150 (MQ=255)
                                             aTGACTGGTGGAATGCAGACCAATAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTc                                                                                                   <  2:789868/150‑1 (MQ=255)
                                                      ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                          <  1:1600167/150‑1 (MQ=255)
                                                       gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                         >  2:607310/1‑150 (MQ=255)
                                                           gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                     >  2:1493414/1‑150 (MQ=255)
                                                             aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAcc                                                                                   <  1:52486/150‑1 (MQ=255)
                                                                  aaTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGAtt                                                                              >  1:1670086/1‑150 (MQ=255)
                                                                     aaCCAAATTCTCTAAATTATAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCAGGTTTATTATTAGAAAAGATGATTATGCTACTTCTTAGTACTGAGGAACCGGATTACg                                                                           >  2:50956/1‑150 (MQ=255)
                                                                     aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           >  2:1444059/1‑150 (MQ=255)
                                                                     aaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACg                                                                           <  1:414786/150‑1 (MQ=255)
                                                                       ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                         >  1:788905/1‑150 (MQ=255)
                                                                        cAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGAGTTCCATACAAAAGAATAAAAAAACCGGAGCCAAATGTACATTCGACTCCGGTTAATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGCATTACCGaa                                                                        >  1:1403353/1‑150 (MQ=255)
                                                                        aaaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGaa                                                                        <  1:143755/149‑1 (MQ=255)
                                                                                ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:393949/1‑150 (MQ=255)
                                                                                ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  2:1557299/150‑1 (MQ=255)
                                                                                ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  1:578972/1‑150 (MQ=255)
                                                                                ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                >  2:1527886/1‑150 (MQ=255)
                                                                                ctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               <  1:991978/150‑1 (MQ=255)
                                                                                atAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTgg                                                                <  1:1461256/149‑1 (MQ=255)
                                                                                 tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                               >  1:1601339/1‑150 (MQ=255)
                                                                                 tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTAATTCGAATCCGGTTTATTATTATAAAATATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACTGAAGCTCTTGGt                                                               >  1:248533/1‑150 (MQ=255)
                                                                                  aaaTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTg                                                              >  2:1560855/1‑150 (MQ=255)
                                                                                    aTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGAc                                                            >  1:377827/1‑150 (MQ=255)
                                                                                        gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACTCaa                                                        >  1:1663394/1‑150 (MQ=255)
                                                                                         aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGTTGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAg                                                       >  2:342802/1‑150 (MQ=255)
                                                                                          aCAAATGGTTTTTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGc                                                      >  1:253832/1‑150 (MQ=255)
                                                                                             aaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAg                                                   <  1:1419624/150‑1 (MQ=255)
                                                                                                    attaATGAGGAGTTCGTTACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATGATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                            <  1:195236/150‑1 (MQ=255)
                                                                                                     ataATGAGGTGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                           <  1:336493/149‑1 (MQ=255)
                                                                                                      taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCTGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCat                                          >  1:704330/1‑149 (MQ=255)
                                                                                                        aTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAc                                        <  1:1285180/150‑1 (MQ=255)
                                                                                                        aTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAc                                        <  1:417172/150‑1 (MQ=255)
                                                                                                         tGAGGAGTTCGATACAAAATAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  2:697210/150‑1 (MQ=255)
                                                                                                         agaggatTTCGAAACAAAAGAATAAAAAACCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                       <  1:1605594/149‑1 (MQ=255)
                                                                                                                   gATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGcc                             <  2:381420/150‑1 (MQ=255)
                                                                                                                    atccAAAAGAATAAACAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCTTACTGCGGAACCGGATTACGTAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGccc                            <  1:912629/147‑1 (MQ=255)
                                                                                                                    aTACAAAAGAATTAAAAAACAGGAGCCAAATGTTCATTCGAATCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGccc                            <  1:84195/150‑1 (MQ=255)
                                                                                                                      aCAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTTGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAg                          >  2:1226182/1‑150 (MQ=255)
                                                                                                                       cAAAAGAATAAAAAAACCGGAGCCAAATGTTCATGCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCGTCGTCCTGCGGACCCGGAGTCCGGAGGCTCTTGGTGACGCAAGCCAGGTAACTCAGACGGATAGCAGCCCAGa                         >  1:362039/1‑150 (MQ=255)
                                                                                                                        aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                        >  2:529878/1‑150 (MQ=255)
                                                                                                                         aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGATCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       >  1:1704677/1‑150 (MQ=255)
                                                                                                                         aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTATATCAGACCGATAGCAGCCCAGATc                       >  2:1540475/1‑150 (MQ=255)
                                                                                                                         aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       <  1:652929/150‑1 (MQ=255)
                                                                                                                         aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                       <  1:284282/150‑1 (MQ=255)
                                                                                                                         aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATc                       >  1:1705660/1‑150 (MQ=255)
                                                                                                                           aGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAg                     >  2:1304093/1‑150 (MQ=255)
                                                                                                                             taataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCACGTAAATCAGACCGATAGCAGCCCAGATCAGAc                   >  2:1622528/8‑150 (MQ=38)
                                                                                                                              aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                  >  2:611352/1‑150 (MQ=255)
                                                                                                                              aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                  >  2:1075424/1‑150 (MQ=255)
                                                                                                                               ataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                 >  1:7568/6‑150 (MQ=38)
                                                                                                                                  ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGa              >  1:1608786/3‑150 (MQ=38)
                                                                                                                                  aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTTCGGAACCGGATTACGGAAGCTCATGGTGACTCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa              >  2:203803/1‑150 (MQ=255)
                                                                                                                                  aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa              <  2:666939/150‑1 (MQ=255)
                                                                                                                                       ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAt         >  1:25503/1‑150 (MQ=255)
                                                                                                                                        cGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGCCCCAGAACCAtt        >  2:377076/1‑150 (MQ=255)
                                                                                                                                        cGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAtt        <  2:1277386/150‑1 (MQ=255)
                                                                                                                                         ggAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTg       >  1:1723525/1‑150 (MQ=255)
                                                                                                                                            gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc    >  1:661341/1‑150 (MQ=255)
                                                                                                                                              cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATAAGCCCCAGAACCATTGAGCtt  >  2:560294/1‑150 (MQ=255)
                                                                                                                                              cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACAGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCtt  >  1:564586/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
AACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTT  >  NC_000913/2085521‑2085812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: