Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,847 G→T 7.7% A17E (GCG→GAG)  wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8470GT 7.7% 363.2 / 9.8 130A17E (GCG→GAG) wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (62/56);  new base T (4/8);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

AACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAC  >  NC_000913/2115699‑2115996
                                                                                                                                                    |                                                                                                                                                     
aaCCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAgcgc                                                                                                                                                      <  1:1757595/150‑1 (MQ=255)
   cAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGGGAAAGGGGTATGGAGGAAGGCAAGCGCCGa                                                                                                                                                   >  1:1101635/1‑150 (MQ=255)
   cAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGa                                                                                                                                                   >  2:40077/1‑150 (MQ=255)
    aaTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGAGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGCAGGCAAGCGCCgcg                                                                                                                                                  >  2:1057925/1‑148 (MQ=255)
        cGGCAGGCACGACGACTGTTTGCCCTTCGGTGAAGGCTTGTTTAAATGAGGCGATCCAGGCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTg                                                                                                                                              <  2:3063/150‑1 (MQ=255)
         ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                             >  2:1185859/1‑150 (MQ=255)
           caggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGAGCCAGTCGTGTGGGTGGTAGTCGCTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCg                                                                                                                                           <  1:100232/150‑1 (MQ=255)
            aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                          <  2:1283563/150‑3 (MQ=255)
            aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                          <  1:1576312/150‑3 (MQ=255)
            aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGt                                                                                                                                          <  1:40077/150‑1 (MQ=255)
             ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                         >  1:196537/1‑150 (MQ=255)
              gcaCGACGAGTGTTTGCCCTTCGCTGATGGATTGTTTAACTGAGGCTATCCAGTCGTGTGGGTTGTAGTCGTTTATCTTAAGGCTTTGTCGGGCGGGAAGCGCGCGGGCGTAAGGGGTATGGAGGAAGGCATGCTCGGCGCTTGCCTTcc                                                                                                                                        <  2:753768/150‑2 (MQ=255)
              gcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcc                                                                                                                                        <  1:1395393/150‑2 (MQ=255)
              gcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCa                                                                                                                                        <  2:200151/150‑1 (MQ=255)
               caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                       <  1:971314/150‑1 (MQ=255)
               caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTGAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGATAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                       <  2:53013/150‑1 (MQ=255)
               caCGACGACTGGTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcct                                                                                                                                       <  2:1503880/150‑3 (MQ=255)
                   acgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATTGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAAc                                                                                                                                   >  1:1147317/1‑150 (MQ=255)
                   acgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGGTTTTTCGGGCGGGAAGCGCGCCGGCGAAAGTGGTATTGAGGAAGGGAATCGCCGAGATTTCCGTAAGGaaa                                                                                                                                   >  1:1333671/1‑149 (MQ=255)
                    cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                  >  1:46379/1‑150 (MQ=255)
                    cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCATTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                  >  2:1691855/1‑150 (MQ=255)
                      acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                <  1:1120918/150‑1 (MQ=255)
                          tttGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACATGCGTc                                                                                                                            <  2:551462/150‑1 (MQ=255)
                            tGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCATTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTAgg                                                                                                                          >  1:162247/1‑150 (MQ=255)
                             gCCCTTCGCTTAAGTCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCggg                                                                                                                         <  2:13339/150‑1 (MQ=255)
                             gCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCggg                                                                                                                         <  2:207975/150‑1 (MQ=255)
                             gCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTTTAGGCGGTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCggg                                                                                                                         <  1:1463269/150‑1 (MQ=255)
                              cccTTCGCTGACGGCTTGTTTAAATGAGCCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGga                                                                                                                        <  1:1278494/150‑1 (MQ=255)
                                  tCGCTGAAGGCTTGTGTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGt                                                                                                                    <  1:1019568/150‑1 (MQ=255)
                                    gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                  <  1:253368/150‑1 (MQ=255)
                                      tgcaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                <  1:370424/147‑1 (MQ=255)
                                      tGAAGGCTTGTTTAAATGTGGCGATCCTGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGGGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                <  2:845089/150‑1 (MQ=255)
                                      tGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                <  2:587758/150‑1 (MQ=255)
                                       gAAGGCTTGTTTAAATGAGGCGATCCAGGCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                               <  1:318346/150‑1 (MQ=255)
                                        caGGGTTGTTTAAATGAGGCGAGCCAGGCGTGTGGGGGGTTGTGGTTATGGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                              <  1:295584/149‑1 (MQ=255)
                                            cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGATATGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                          >  2:2728/1‑150 (MQ=255)
                                            cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                          >  1:35079/1‑150 (MQ=255)
                                              tGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGc                                                                                                        <  2:532560/150‑1 (MQ=255)
                                               gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTAGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGTAAGGAAAGCGCCGAGCTTGCCGTAAGGAACGTGCGTCGGTAGAGTTTTTTAAATGGCa                                                                                                       >  1:1281289/1‑150 (MQ=255)
                                               gTTTAAATGAGGCGATCCAGTCGTGTGGGTAGTAGTCGTTAATGTTTACGCTTTGTCGGGCGTGAATCGCGCGGGCGTAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       <  2:348076/150‑1 (MQ=255)
                                                tttAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTGGGTTATGTTAACGCGTTGTGGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAATCGCCGAGCTTGCCGTGAGGGGCGGGCGGGGGGGGAGTTTTTTAAATGGcct                                                                                                      >  2:1581685/1‑148 (MQ=255)
                                                     aTGAGGCGATCCAGTCGGGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGtt                                                                                                 <  2:436263/150‑1 (MQ=255)
                                                         ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGTTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                             <  1:935894/150‑1 (MQ=255)
                                                            gATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCctct                                                                                          >  1:1010680/1‑150 (MQ=255)
                                                             aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                         >  2:41487/1‑150 (MQ=255)
                                                             aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACCCTTTGTAGGGCGGGAAGCCCGCGGGCGAAAAGGGTATTGAGGAAGGCAAACGCCGAGCATGCCGTAAAGAACGTGCGTCGGGAGAGTTTTTTAAAATGCATGCGGTCTACTCta                                                                                         >  1:1375224/1‑150 (MQ=255)
                                                              tCCAGTCGTGTGGGTTGTATTCGTTACTGTTAACGATTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                        <  1:341591/150‑1 (MQ=255)
                                                                    cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                  <  1:1276872/150‑1 (MQ=255)
                                                                    cGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGAGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                  <  2:870621/150‑1 (MQ=255)
                                                                       gtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                               >  1:312875/1‑150 (MQ=255)
                                                                        tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                              <  2:1070032/150‑1 (MQ=255)
                                                                        tgGGTTGTAGTCGATAATGTTAACGCTGTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                              <  2:1114057/150‑1 (MQ=255)
                                                                         gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGACCGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                             >  2:1169048/1‑150 (MQ=255)
                                                                              gTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGc                                                                        >  2:1131910/1‑150 (MQ=255)
                                                                               tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCt                                                                       >  1:1444455/1‑150 (MQ=255)
                                                                                aGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTg                                                                      <  2:210310/150‑1 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   <  1:645238/150‑1 (MQ=255)
                                                                                     ttAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                 <  1:1016296/150‑1 (MQ=255)
                                                                                     ttAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                 >  1:606296/1‑150 (MQ=255)
                                                                                        aTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtc                                                              >  1:1635616/1‑150 (MQ=255)
                                                                                         tGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                             >  1:353834/1‑150 (MQ=255)
                                                                                          gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGACCGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                            >  1:987310/1‑150 (MQ=255)
                                                                                          gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGGCTGCAGCAAGCTGGAGAGTtcta                                                            >  1:110733/1‑149 (MQ=255)
                                                                                           ttAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCg                                                           >  1:1778320/1‑150 (MQ=255)
                                                                                           ttAACGCGTTGTCGGGCGGTAATCGCGCGGGCGGAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGTAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCg                                                           <  2:1676485/150‑1 (MQ=255)
                                                                                                gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                      >  2:1277043/1‑150 (MQ=255)
                                                                                                 cTTTGTCGGGCGGGAAGCTCGCGTGCGAAAGGGGTTTGGTCGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGGTTTTTAAATGGCATGCGGTCTCCGCTATAAATCCTGCAGCATGCTGGCGAGTTCTCGATTGAt                                                     <  1:120566/150‑1 (MQ=255)
                                                                                                  tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTGTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAta                                                    >  1:288956/1‑149 (MQ=255)
                                                                                                  tttGTCGGGAGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                    >  2:1066369/1‑150 (MQ=255)
                                                                                                   ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCa                                                   <  2:162247/150‑1 (MQ=255)
                                                                                                     gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCAGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCAcc                                                 >  2:1199985/1‑150 (MQ=255)
                                                                                                           cgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTgg                                           <  1:848053/150‑1 (MQ=255)
                                                                                                            gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                          >  2:800948/1‑150 (MQ=255)
                                                                                                            gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                          >  1:413419/1‑150 (MQ=255)
                                                                                                              gAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                        >  2:1774120/1‑150 (MQ=255)
                                                                                                                aGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACATGCTGGTTaaa                                      >  2:860430/1‑150 (MQ=255)
                                                                                                                  cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAt                                    <  2:646462/150‑1 (MQ=255)
                                                                                                                      cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                >  2:344041/1‑150 (MQ=255)
                                                                                                                          cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                            >  2:26390/1‑150 (MQ=255)
                                                                                                                          cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                            >  2:296913/1‑150 (MQ=255)
                                                                                                                               ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                       >  1:29190/1‑150 (MQ=255)
                                                                                                                               ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                       >  2:1523574/1‑150 (MQ=255)
                                                                                                                               ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                       >  1:538822/1‑150 (MQ=255)
                                                                                                                               ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                       >  1:531888/1‑150 (MQ=255)
                                                                                                                                 ggTATGGAGGAAGGCCAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                     <  1:181594/150‑1 (MQ=255)
                                                                                                                                    aTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgc                  >  1:1458480/1‑150 (MQ=255)
                                                                                                                                     tGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCAGGTTAAAATCGTGTTCAACTATTTcgcg                 >  1:1204151/1‑150 (MQ=255)
                                                                                                                                     tGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGATTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcg                 >  2:187129/1‑150 (MQ=255)
                                                                                                                                      ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                <  2:46379/150‑1 (MQ=255)
                                                                                                                                       gaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCATCTTTTTcgcgcg               >  2:1751235/1‑150 (MQ=255)
                                                                                                                                       cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCAGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTTTTCAAATTTTTcgcgcg               >  2:1490789/7‑150 (MQ=255)
                                                                                                                                        ctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc              >  2:1120790/6‑150 (MQ=255)
                                                                                                                                           acggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCACCTCTATAAAGCCTGCAGCAAGTTGGCGAGTTCTCGATTGAGCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg           >  2:742812/3‑150 (MQ=255)
                                                                                                                                            aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt          >  2:1628407/1‑150 (MQ=255)
                                                                                                                                               cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg       <  1:765515/150‑1 (MQ=255)
                                                                                                                                               cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg       >  1:1753535/1‑150 (MQ=255)
                                                                                                                                               cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGGTCTCGGCTATAAAGCCTGCAGCACGCTGGCGAGTTCTGGATTGATCACCTGCGGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGGTTg       >  2:276480/1‑150 (MQ=255)
                                                                                                                                                 aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgac     >  2:47569/1‑150 (MQ=255)
                                                                                                                                                    gcCGAGCTTGCCGTCATGAAAGTGCGTCGGGAGAGTTTATATATTGGGATGCTTTCTCCTCTATAAAGCCTGAAGCACGCTGGCGAGTTCTCGATTGATCTCCTTCTGGTTAAAAACGTGTTCTACTTTTTCGCGCGCGCGTTTgacgac  <  2:1664110/150‑1 (MQ=255)
                                                                                                                                                    gcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgac  >  2:607511/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAC  >  NC_000913/2115699‑2115996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: