Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,849 C→G 6.8% S16S (TCG→TCC) ‡ wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8490CG 6.8% 434.3 / 18.1 132S16S (TCG→TCC) ‡wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (64/59);  new base G (3/6);  total (67/65)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 134 total aligned reads displayed.

CAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115702‑2115997
                                                                                                                                                   |                                                                                                                                                    
cAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGGGAAAGGGGTATGGAGGAAGGCAAGCGCCGa                                                                                                                                                    >  1:1101635/1‑150 (MQ=255)
 aaTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGAGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGCAGGCAAGCGCCgcg                                                                                                                                                   >  2:1057925/1‑148 (MQ=255)
  aTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGc                                                                                                                                                  <  1:1143241/150‑1 (MQ=255)
        caggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGAGCCAGTCGTGTGGGTGGTAGTCGCTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCg                                                                                                                                            <  1:100232/150‑1 (MQ=255)
         aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                           <  1:1576312/150‑3 (MQ=255)
         aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGt                                                                                                                                           <  1:40077/150‑1 (MQ=255)
          ggcaCGACGTCTGGTTGCCCTGCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                          <  2:1089512/150‑1 (MQ=255)
          ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                          >  1:196537/1‑150 (MQ=255)
           gcaCGACGAGTGTTTGCCCTTCGCTGATGGATTGTTTAACTGAGGCTATCCAGTCGTGTGGGTTGTAGTCGTTTATCTTAAGGCTTTGTCGGGCGGGAAGCGCGCGGGCGTAAGGGGTATGGAGGAAGGCATGCTCGGCGCTTGCCTTcc                                                                                                                                         <  2:753768/150‑2 (MQ=255)
           gcaCGACGACTGTTTGCCCTTCTCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcc                                                                                                                                         <  1:1255540/150‑2 (MQ=255)
           gcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCa                                                                                                                                         <  2:200151/150‑1 (MQ=255)
            caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                        <  1:971314/150‑1 (MQ=255)
                acgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATTGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAAc                                                                                                                                    >  1:1147317/1‑150 (MQ=255)
                acgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGGTTTTTCGGGCGGGAAGCGCGCCGGCGAAAGTGGTATTGAGGAAGGGAATCGCCGAGATTTCCGTAAGGaaa                                                                                                                                    >  1:1333671/1‑149 (MQ=255)
                 cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCATTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                   >  2:1691855/1‑150 (MQ=255)
                     tGTTTGCCCTTCGCTGAAGGCTTGTTTAATTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTACTGTTAAGGCTTTGTCGGGCGGGAAGCGCGCGTGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCg                                                                                                                               <  1:270901/150‑1 (MQ=255)
                       tttGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACATGCGTc                                                                                                                             <  2:551462/150‑1 (MQ=255)
                          gCCCTTCGCTTAAGTCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCggg                                                                                                                          <  2:13339/150‑1 (MQ=255)
                          gCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCggg                                                                                                                          <  1:374097/150‑1 (MQ=255)
                           cccTTCGCTGACGGCTTGTTTAAATGAGCCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGga                                                                                                                         <  1:1278494/150‑1 (MQ=255)
                             cTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCATTGTCGGGCCGTAAGCGCTCGGGCGAAAGTGGTATGGAGGAAGGCAAGCGCCGAGCTTTCCGTCAGGAACGTGAGTCGGgagt                                                                                                                       >  2:120566/1‑149 (MQ=255)
                                    gAAGGCTTGTTTAAATGAGGCGATCCAGGCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                                <  1:318346/150‑1 (MQ=255)
                                         cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                           >  1:35079/1‑150 (MQ=255)
                                           tGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGc                                                                                                         <  2:532560/150‑1 (MQ=255)
                                            gTTTAAATGAGGCGATCCAGTCGTGTGGGTAGTAGTCGTTAATGTTTACGCTTTGTCGGGCGTGAATCGCGCGGGCGTAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                        <  2:348076/150‑1 (MQ=255)
                                             tttAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTGGGTTATGTTAACGCGTTGTGGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAATCGCCGAGCTTGCCGTGAGGGGCGGGCGGGGGGGGAGTTTTTTAAATGGcct                                                                                                       >  2:1581685/1‑148 (MQ=255)
                                                aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                    >  2:713855/1‑150 (MQ=255)
                                                      ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGTTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                              <  1:935894/150‑1 (MQ=255)
                                                      ggCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                              <  2:1532904/150‑1 (MQ=255)
                                                       gCGAACCAGTCGTGGGGGGTGTAGTCGGTAATGTTTACGCGGTGTCGGGGGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCCAGCGCCGCGCTTGGCGGCCGGAACGGGCGTGGGGTGAGTTTTTTAAATGGCAAGGGTTgtccg                                                                                             >  2:1749418/1‑149 (MQ=255)
                                                         gATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCctct                                                                                           >  1:1010680/1‑150 (MQ=255)
                                                          aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                          >  2:41487/1‑150 (MQ=255)
                                                          aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACCCTTTGTAGGGCGGGAAGCCCGCGGGCGAAAAGGGTATTGAGGAAGGCAAACGCCGAGCATGCCGTAAAGAACGTGCGTCGGGAGAGTTTTTTAAAATGCATGCGGTCTACTCta                                                                                          >  1:1375224/1‑150 (MQ=255)
                                                          aTCCAGTCGTGGGGGTTGTAGTCGGTAATGTTCCCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                          <  2:249588/150‑1 (MQ=255)
                                                           tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                         <  1:1713211/150‑1 (MQ=255)
                                                           tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                         <  1:640157/150‑1 (MQ=255)
                                                           tCCAGGCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                         <  1:349943/150‑1 (MQ=255)
                                                                 cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                   <  1:1276872/150‑1 (MQ=255)
                                                                 cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                   >  1:479997/1‑150 (MQ=255)
                                                                 cGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGAGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                   <  2:870621/150‑1 (MQ=255)
                                                                    gtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                >  1:312875/1‑150 (MQ=255)
                                                                     tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1070032/150‑1 (MQ=255)
                                                                     tgGGTTGTAGTCGATAATGTTAACGCTGTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1114057/150‑1 (MQ=255)
                                                                      gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGACCGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                              >  2:1169048/1‑150 (MQ=255)
                                                                       ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                             <  2:1466926/150‑1 (MQ=255)
                                                                           gTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGc                                                                         >  2:1131910/1‑150 (MQ=255)
                                                                           gTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGc                                                                         >  2:835718/1‑150 (MQ=255)
                                                                            tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCt                                                                        >  1:1444455/1‑150 (MQ=255)
                                                                            tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCt                                                                        <  2:196537/150‑1 (MQ=255)
                                                                             aGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTg                                                                       <  2:210310/150‑1 (MQ=255)
                                                                                cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    <  1:1411464/150‑1 (MQ=255)
                                                                                cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    <  1:1503761/150‑1 (MQ=255)
                                                                                cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    <  1:645238/150‑1 (MQ=255)
                                                                                 gTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATTGAGGAAGGCAAGCGCCGAGCTTGCAGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGa                                                                   >  1:900695/1‑150 (MQ=255)
                                                                                  ttAATTTTACCGCTGTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGATTTTATTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                  <  1:1793812/150‑1 (MQ=255)
                                                                                  ttAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                  <  1:1016296/150‑1 (MQ=255)
                                                                                     aTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtc                                                               >  1:1635616/1‑150 (MQ=255)
                                                                                      tGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                              >  1:353834/1‑150 (MQ=255)
                                                                                       gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGGCTGCAGCAAGCTGGAGAGTtcta                                                             >  1:110733/1‑149 (MQ=255)
                                                                                       gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                             <  1:1298117/150‑1 (MQ=255)
                                                                                             gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                       >  2:1277043/1‑150 (MQ=255)
                                                                                               tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTGTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAta                                                     >  1:288956/1‑149 (MQ=255)
                                                                                                  gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCAGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCAcc                                                  >  2:1199985/1‑150 (MQ=255)
                                                                                                      ggcgggAAGCGTGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGAGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgcg                                              >  2:347838/1‑149 (MQ=255)
                                                                                                      ggcgggAAGCGTGCGGGCGAAAGGGGTATCGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGAGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgct                                              >  2:485766/1‑150 (MQ=255)
                                                                                                      ggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgct                                              >  2:431329/1‑150 (MQ=255)
                                                                                                        cgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTgg                                            <  2:100553/150‑1 (MQ=255)
                                                                                                         gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                           >  1:413419/1‑150 (MQ=255)
                                                                                                         gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                           >  2:800948/1‑150 (MQ=255)
                                                                                                          ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                          >  2:29449/1‑150 (MQ=255)
                                                                                                          ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGAAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                          >  1:1078378/1‑150 (MQ=255)
                                                                                                             aGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACATGCTGGTTaaa                                       >  2:860430/1‑150 (MQ=255)
                                                                                                               cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAt                                     <  2:646462/150‑1 (MQ=255)
                                                                                                               cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCGTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAt                                     <  2:1147317/150‑1 (MQ=255)
                                                                                                                   cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                 >  2:344041/1‑150 (MQ=255)
                                                                                                                       cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  2:296913/1‑150 (MQ=255)
                                                                                                                       cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  2:26390/1‑150 (MQ=255)
                                                                                                                            ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        >  1:538822/1‑150 (MQ=255)
                                                                                                                            ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        >  1:531888/1‑150 (MQ=255)
                                                                                                                            ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        >  1:29190/1‑150 (MQ=255)
                                                                                                                            ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        >  2:1523574/1‑150 (MQ=255)
                                                                                                                              ggTATGGAGGAAGGCCAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                      <  1:181594/150‑1 (MQ=255)
                                                                                                                                 aTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgc                   >  1:1458480/1‑150 (MQ=255)
                                                                                                                                  tGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCAGGTTAAAATCGTGTTCAACTATTTcgcg                  >  1:1204151/1‑150 (MQ=255)
                                                                                                                                  tGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGATTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcg                  >  2:187129/1‑150 (MQ=255)
                                                                                                                                   ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                 <  2:46379/150‑1 (MQ=255)
                                                                                                                                   ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                 <  1:296913/150‑1 (MQ=255)
                                                                                                                                    gaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCATCTTTTTcgcgcg                >  2:1751235/1‑150 (MQ=255)
                                                                                                                                    cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCAGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTTTTCAAATTTTTcgcgcg                >  2:1490789/7‑150 (MQ=255)
                                                                                                                                         aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt           >  2:1635668/1‑150 (MQ=255)
                                                                                                                                         aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt           >  2:1628407/1‑150 (MQ=255)
                                                                                                                                         aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt           >  2:572264/1‑150 (MQ=255)
                                                                                                                                            cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg        <  1:765515/150‑1 (MQ=255)
                                                                                                                                            cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg        >  1:1753535/1‑150 (MQ=255)
                                                                                                                                            cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGGTCTCGGCTATAAAGCCTGCAGCACGCTGGCGAGTTCTGGATTGATCACCTGCGGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGGTTg        >  2:276480/1‑150 (MQ=255)
                                                                                                                                              aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgac      >  2:47569/1‑150 (MQ=255)
                                                                                                                                                 gcCGAGCTTGCCGTCATGAAAGTGCGTCGGGAGAGTTTATATATTGGGATGCTTTCTCCTCTATAAAGCCTGAAGCACGCTGGCGAGTTCTCGATTGATCTCCTTCTGGTTAAAAACGTGTTCTACTTTTTCGCGCGCGCGTTTgacgac   <  2:1664110/150‑1 (MQ=255)
                                                                                                                                                 gcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgac   >  2:607511/1‑150 (MQ=255)
                                                                                                                                                  ccGATCTTTCCGTCAGGAACGTGCGTCGGGAGAGTTTTTGAAATGGCATGCGTTCTCCTCTATAAACCCTGCAGCAAGATGGCGTTTGATCGATTGATCACCTGCTGGTTAAAATCGTGTTCAAATTTTACGCGCGCGCGTTTGACGAcc  >  2:428930/1‑150 (MQ=255)
                                                                                                                                                  ccGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAcc  >  1:1536892/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115702‑2115997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: